| 质粒名称: | lenti MS2-P65-HSF1_Hygro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 61426 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | plenti |
| 载体抗性: | 氨苄霉素 |
| 载体类型: | 哺乳细胞表达,慢病毒 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MS2-P65-HSF1_2A_Hygro |
| 物种来源: | H. sapiens (human), Synthetic |
| 基因突变: | N55K in MS2 |
| 启动子 | EF1A |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BsiWI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | GTT TGG ATC TTG GTT CAT TCT CAA GCC TCA G |
| 3' 测序引物 | cacatagcgtaaaaggagcaacatag |
|
文献引用方法 |
|
| 材料和方法部分: | lenti MS2-P65-HSF1_Hygro was a gift from Feng Zhang (Addgene plasmid # 61426) |
| 参考文献部分: | Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS lenti MS2-P65-HSF1_Hygro Sequencing Result 11736 bp DNA SYN
DEFINITION lenti MS2-P65-HSF1_Hygro Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..11736
/organism="lenti MS2-P65-HSF1_Hygro Sequencing Result"
/mol_type="other DNA"
promoter 239..815
/label="CMV_immearly_promoter"
misc_feature 318..605
/label="CAG_enhancer"
misc_feature 772..792
/label="CMV_fwd_primer"
misc_feature 835..1015
/label="truncHIV-1_3_LTR"
misc_feature 835..1015
/label="HIV-1_5_LTR"
misc_feature 1126..1170
/label="HIV-1_psi_pack"
CDS 1558..2445
/label="ORF frame 1"
misc_feature 1680..1913
/label="RRE"
misc_feature 2444..2459
/label="cPPT"
promoter 2694..3865
/label="EF1a_promoter"
CDS complement(2985..3605)
/label="ORF frame 2"
misc_feature 3813..3833
/label="EF1a_fwd_primer"
CDS 3878..6400
/label="ORF frame 2"
misc_feature 4322..4342
/label="SV40 NLS"
misc_feature 4328..4342
/label="NLS"
CDS complement(5330..6127)
/label="ORF frame 3"
gene 5396..6355
/label="hygroB (36 - 995)"
/gene="hygroB (36 - 995)"
misc_feature 6430..7017
/label="WPRE"
CDS 6518..7567
/label="ORF frame 2"
CDS 6531..7043
/label="ORF frame 3"
misc_feature complement(7020..7036)
/label="pBluescriptKS_primer"
misc_feature 7204..7225
/label="U3PPT"
misc_feature 7204..7219
/label="cPPT"
misc_feature 7541..7721
/label="HIV-1_5_LTR"
misc_feature 7541..7721
/label="truncHIV-1_3_LTR"
misc_feature complement(7747..7764)
/label="BGH_rev_primer"
terminator 7750..7977
/label="bGH_PA_terminator"
rep_origin 8040..8346
/label="f1_origin"
misc_feature complement(8460..8480)
/label="pBABE_3_primer"
misc_feature complement(8466..8681)
/label="SV40_enhancer"
promoter 8478..8746
/label="SV40_promoter"
rep_origin 8645..8722
/label="SV40_origin"
misc_feature 8707..8726
/label="SV40pro_F_primer"
promoter 8840..8907
/label="EM7_promoter"
misc_feature 8908..9282
/label="sh_ble"
gene 8908..9279
/label="bleo"
/gene="bleo"
terminator 9415..9534
/label="SV40_PA_terminator"
misc_feature 9503..9522
/label="EBV_rev_primer"
promoter complement(9578..9596)
/label="M13_reverse_primer"
misc_feature complement(9595..9617)
/label="M13_pUC_rev_primer"
promoter complement(9631..9660)
/label="lac_promoter"
gene complement(10743..11603)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(10743..11603)
/label="ORF frame 2"
promoter complement(11645..11673)
/label="AmpR_promoter"
ORIGIN
1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC
241 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
301 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
361 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
421 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
481 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
541 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
601 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT
661 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC
721 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG
781 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT
841 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT
901 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC
961 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC
1021 GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC
1081 GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA
1141 TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG
1201 GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA
1261 AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC
1321 TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA
1381 CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC
1441 AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA
1501 AAAGTAAGAC CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG
1561 AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA
1621 GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA
1681 GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG
1741 ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG
1801 CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG
1861 CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT
1921 TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT
1981 AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT
2041 AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG
2101 AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA
2161 ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA
2221 AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA
2281 TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA
2341 GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC GGCACTGCGT
2401 GCGCCAATTC TGCAGACAAA TGGCAGTATT CATCCACAAT TTTAAAAGAA AAGGGGGGAT
2461 TGGGGGGTAC AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA
2521 AGAATTACAA AAACAAATTA CAAAAATTCA AAATTTTCGG GTTTATTACA GGGACAGCAG
2581 AGATCCAGTT TGGTTAATTA GCTAGCTGCA AAGATGGATA AAGTTTTAAA CAGAGAGGAA
2641 TCTTTGCAGC TAATGGACCT TCTAGGTCTT GAAAGGAGTG GGAATTGGCT CCGGTGCCCG
2701 TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA
2761 TTGAACCGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG
2821 GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA
2881 CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGGTA AGTGCCGTGT GTGGTTCCCG
2941 CGGGCCTGGC CTCTTTACGG GTTATGGCCC TTGCGTGCCT TGAATTACTT CCACCTGGCT
3001 GCAGTACGTG ATTCTTGATC CCGAGCTTCG GGTTGGAAGT GGGTGGGAGA GTTCGAGGCC
3061 TTGCGCTTAA GGAGCCCCTT CGCCTCGTGC TTGAGTTGAG GCCTGGCCTG GGCGCTGGGG
3121 CCGCCGCGTG CGAATCTGGT GGCACCTTCG CGCCTGTCTC GCTGCTTTCG ATAAGTCTCT
3181 AGCCATTTAA AATTTTTGAT GACCTGCTGC GACGCTTTTT TTCTGGCAAG ATAGTCTTGT
3241 AAATGCGGGC CAAGATCTGC ACACTGGTAT TTCGGTTTTT GGGGCCGCGG GCGGCGACGG
3301 GGCCCGTGCG TCCCAGCGCA CATGTTCGGC GAGGCGGGGC CTGCGAGCGC GGCCACCGAG
3361 AATCGGACGG GGGTAGTCTC AAGCTGGCCG GCCTGCTCTG GTGCCTGGCC TCGCGCCGCC
3421 GTGTATCGCC CCGCCCTGGG CGGCAAGGCT GGCCCGGTCG GCACCAGTTG CGTGAGCGGA
3481 AAGATGGCCG CTTCCCGGCC CTGCTGCAGG GAGCTCAAAA TGAAGGACGC GGCGCTCGGG
3541 AGAGCGGGCG GGTGAGTCAC CCACACAAAG GAAAAGGGCC TTTCCGTCCT CAGCCGTCGC
3601 TTCATGTGAC TCCACGGAGT ACCGGGCGCC GTCCAGGCAC CTCGATTAGT TCTCGAGCTT
3661 TTGGAGTACG TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC
3721 TGAGTGGGTG GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT
3781 TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT
3841 TTTTCTTCCA TTTCAGGTGT CGTGACGTAC GGCCACCATG GCTTCAAACT TTACTCAGTT
3901 CGTGCTCGTG GACAATGGTG GGACAGGGGA TGTGACAGTG GCTCCTTCTA ATTTCGCTAA
3961 TGGGGTGGCA GAGTGGATCA GCTCCAACTC ACGGAGCCAG GCCTACAAGG TGACATGCAG
4021 CGTCAGGCAG TCTAGTGCCC AGAAGAGAAA GTATACCATC AAGGTGGAGG TCCCCAAAGT
4081 GGCTACCCAG ACAGTGGGCG GAGTCGAACT GCCTGTCGCC GCTTGGAGGT CCTACCTGAA
4141 CATGGAGCTC ACTATCCCAA TTTTCGCTAC CAATTCTGAC TGTGAACTCA TCGTGAAGGC
4201 AATGCAGGGG CTCCTCAAAG ACGGTAATCC TATCCCTTCC GCCATCGCCG CTAACTCAGG
4261 TATCTACAGC GCTGGAGGAG GTGGAAGCGG AGGAGGAGGA AGCGGAGGAG GAGGTAGCGG
4321 ACCTAAGAAA AAGAGGAAGG TGGCGGCCGC TGGATCCCCT TCAGGGCAGA TCAGCAACCA
4381 GGCCCTGGCT CTGGCCCCTA GCTCCGCTCC AGTGCTGGCC CAGACTATGG TGCCCTCTAG
4441 TGCTATGGTG CCTCTGGCCC AGCCACCTGC TCCAGCCCCT GTGCTGACCC CAGGACCACC
4501 CCAGTCACTG AGCGCTCCAG TGCCCAAGTC TACACAGGCC GGCGAGGGGA CTCTGAGTGA
4561 AGCTCTGCTG CACCTGCAGT TCGACGCTGA TGAGGACCTG GGAGCTCTGC TGGGGAACAG
4621 CACCGATCCC GGAGTGTTCA CAGATCTGGC CTCCGTGGAC AACTCTGAGT TTCAGCAGCT
4681 GCTGAATCAG GGCGTGTCCA TGTCTCATAG TACAGCCGAA CCAATGCTGA TGGAGTACCC
4741 CGAAGCCATT ACCCGGCTGG TGACCGGCAG CCAGCGGCCC CCCGACCCCG CTCCAACTCC
4801 CCTGGGAACC AGCGGCCTGC CTAATGGGCT GTCCGGAGAT GAAGACTTCT CAAGCATCGC
4861 TGATATGGAC TTTAGTGCCC TGCTGTCACA GATTTCCTCT AGTGGGCAGG GAGGAGGTGG
4921 AAGCGGCTTC AGCGTGGACA CCAGTGCCCT GCTGGACCTG TTCAGCCCCT CGGTGACCGT
4981 GCCCGACATG AGCCTGCCTG ACCTTGACAG CAGCCTGGCC AGTATCCAAG AGCTCCTGTC
5041 TCCCCAGGAG CCCCCCAGGC CTCCCGAGGC AGAGAACAGC AGCCCGGATT CAGGGAAGCA
5101 GCTGGTGCAC TACACAGCGC AGCCGCTGTT CCTGCTGGAC CCCGGCTCCG TGGACACCGG
5161 GAGCAACGAC CTGCCGGTGC TGTTTGAGCT GGGAGAGGGC TCCTACTTCT CCGAAGGGGA
5221 CGGCTTCGCC GAGGACCCCA CCATCTCCCT GCTGACAGGC TCGGAGCCTC CCAAAGCCAA
5281 GGACCCCACT GTCTCCTGTA CAGGCAGTGG AGAGGGCAGA GGAAGTCTGC TAACATGCGG
5341 TGACGTCGAG GAGAATCCTG GCCCAACCAT GAAAAAGCCT GAACTCACCG CTACCTCTGT
5401 CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC TCTCCGAGGG
5461 CGAAGAATCT CGGGCTTTCA GCTTCGATGT GGGAGGGCGT GGATATGTCC TGCGGGTGAA
5521 TAGCTGCGCC GATGGTTTCT ACAAAGATCG CTATGTTTAT CGGCACTTTG CATCCGCCGC
5581 TCTCCCTATT CCCGAAGTGC TTGACATTGG GGAGTTCAGC GAGAGCCTGA CCTATTGCAT
5641 CTCCCGCCGT GCACAGGGTG TCACCTTGCA AGACCTGCCT GAAACCGAAC TGCCCGCTGT
5701 TCTCCAGCCC GTCGCCGAGG CCATGGATGC CATCGCTGCC GCCGATCTTA GCCAGACCAG
5761 CGGGTTCGGC CCATTCGGAC CTCAAGGAAT CGGTCAATAC ACTACATGGC GCGATTTCAT
5821 CTGCGCTATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG ACACCGTCAG
5881 TGCCTCCGTC GCCCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT GCCCCGAAGT
5941 CCGGCACCTC GTGCACGCCG ATTTCGGCTC CAACAATGTC CTGACCGACA ATGGCCGCAT
6001 AACAGCCGTC ATTGACTGGA GCGAGGCCAT GTTCGGGGAT TCCCAATACG AGGTCGCCAA
6061 CATCTTCTTC TGGAGGCCCT GGTTGGCTTG TATGGAGCAG CAGACCCGCT ACTTCGAGCG
6121 GAGGCATCCC GAGCTTGCAG GATCTCCTCG GCTCCGGGCT TATATGCTCC GCATTGGTCT
6181 TGACCAACTC TATCAGAGCT TGGTTGACGG CAATTTCGAT GATGCAGCTT GGGCTCAGGG
6241 TCGCTGCGAC GCAATCGTCC GGTCCGGAGC CGGGACTGTC GGGCGTACAC AAATCGCCCG
6301 CAGAAGCGCT GCCGTCTGGA CCGATGGCTG TGTGGAAGTG CTCGCCGATA GTGGAAACAG
6361 ACGCCCCAGC ACTCGTCCTA GGGCAAAGGA TCTGCAGTAA TGAGAATTCG ATATCAAGCT
6421 TATCGGTAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA
6481 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC
6541 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA
6601 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC
6661 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC
6721 CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC
6781 TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG
6841 GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC
6901 GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC
6961 GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG
7021 ATACCGTCGA CCTCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC
7081 TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT
7141 CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT
7201 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT
7261 TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG
7321 GCCAGGGATC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA
7381 AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT
7441 GCATGGGATG GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA GCCGCCTAGC
7501 ATTTCATCAC ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT GGTTAGACCA
7561 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG
7621 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG
7681 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGGGCCCGTT TAAACCCGCT
7741 GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC
7801 CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG
7861 CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA
7921 AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT
7981 CTGAGGCGGA AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG
8041 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC
8101 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC
8161 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG
8221 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG
8281 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG
8341 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT
8401 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG
8461 GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA
8521 AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG
8581 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC
8641 GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT
8701 TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG
8761 AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT
8821 TTTCGGATCT GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA
8881 TAATACGACA AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC
8941 ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG
9001 GACTTCGTGG AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC
9061 GCGGTCCAGG ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG
9121 GACGAGCTGT ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG
9181 CCGGCCATGA CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG
9241 GCCGGCAACT GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC
9301 GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC
9361 TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT
9421 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA
9481 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC
9541 TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG
9601 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA
9661 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT
9721 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG cattaatgaa tcggccaacg cgcggggaga
9781 ggcggtttgc gtattgggcg ctCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
9841 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
9901 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
9961 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
10021 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
10081 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
10141 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
10201 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
10261 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
10321 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
10381 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
10441 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
10501 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
10561 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
10621 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
10681 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
10741 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
10801 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
10861 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
10921 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
10981 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
11041 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
11101 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
11161 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
11221 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
11281 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
11341 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
11401 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
11461 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
11521 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
11581 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
11641 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
11701 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
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