| 质粒名称: | LbCpf1-2NLS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 102566 |
| 存储实验室: | Jennifer Doudna |
|
载体骨架基本信息 |
|
| 载体名称: | pET |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 10500 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LbCpf1 |
| 物种来源: | Synthetic |
| 插入大小: | 5000 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | T7 |
| 标签/融合蛋白: | MBP（插入件上的N端子） |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 Pro |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | LbCpf1-2NLS was a gift from Jennifer Doudna (Addgene plasmid # 102566 ; http://n2t.net/addgene:102566 ; RRID:Addgene_102566) |
| 参考文献部分: | CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ. Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2. 10.1038/s41467-017-01836-2 [pii] PubMed 29222508 |
买家导航
LbCpf12NLS载体基本信息
LbCpf12NLS载体图谱质粒图谱和多克隆位点信息
LbCpf12NLS载体简介
LbCpf12NLS载体序列
LOCUS LbCpf1-2NLS 10514 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION bacterial expression of LbCpf1-2NLS.
ACCESSION .
VERSION .
KEYWORDS LbCpf1-2NLS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10514)
AUTHORS Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E,
Khokha MK, Doudna JA, Giraldez AJ
TITLE CRISPR-Cpf1 mediates efficient homology-directed repair and
temperature-controlled genome editing.
JOURNAL Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2.
PUBMED 29222508
REFERENCE 2 (bases 1 to 10514)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10514
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 506..525
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 609..628
/label=T7
/note="T7 promoter, forward primer"
promoter 609..627
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 716..738
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 758..775
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 785..1885
/codon_start=1
/gene="malE (mutated)"
/product="maltose binding protein from E. coli"
/label=MBP
/note="This version of the gene does not encode a signal
sequence, so MBP will remain in the cytosol."
/translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
WYAVRTAVINAASGRQTVDEALKDAQT"
primer_bind 1859..1882
/label=MBP-F
/note="Maltose binding protein, forward primer"
CDS 1940..1960
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
CDS 1970..5653
/codon_start=1
/product="CRISPR-associated protein from Lachnospiraceae
bacterium ND2006 (Zetsche et al., 2015)"
/label=LbCpf1
/translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
VKH"
CDS 5657..5683
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 5702..5722
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5729..5749
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5789..6493
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind complement(5931..5949)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 6199..6218
/label=mCherry-F
/note="mCherry, forward primer"
CDS 6500..6520
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(6652..6670)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 6666..6713
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(7079..7107)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(7134..7152)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7220..7324
/gene="bla"
/label=AmpR promoter
CDS 7325..8185
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7543..7562)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8356..8944
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8845..8864
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9098..9115
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 9130..9269
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(9195..9214)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 9355..9377
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(9371..9562)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
ORIGIN
1 agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac
61 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg
121 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc
181 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg
241 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
301 gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
361 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
421 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
481 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
541 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
601 cgcgaaatta atacgactca ctatagggag accacaacgg tttccctcta gtgccggctc
661 cggagagctc tttaattaag cggccgccct gcaggactcg agttctagaa ataattttgt
721 ttaactttaa gaaggagata tacatatgaa atcttctcac catcaccatc accatggttc
781 ttctatgaaa atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa
841 cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga
901 gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga
961 cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga
1021 aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg
1081 ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa
1141 caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga
1201 actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg
1261 gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat
1321 taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct
1381 gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa
1441 taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag
1501 caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt
1561 cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga
1621 gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc
1681 gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc
1741 cgccactatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc
1801 tttctggtat gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga
1861 tgaagccctg aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa
1921 caacaacctc gggatcgagg aaaacctgta cttccaatcc aatgccacca tgagcaaact
1981 ggagaagttc acgaactgtt attctctgtc gaaaacctta cgttttaagg ccattccggt
2041 aggtaaaacg caagaaaata tcgacaataa acgtctcctg gttgaagatg aaaagcgtgc
2101 cgaagattat aaaggtgtta aaaagctgct tgatcgctat tacctgtctt ttatcaacga
2161 tgttctgcat tccatcaaat tgaaaaactt gaataattac atctcgttat ttcgcaaaaa
2221 aacccgtacc gaaaaagaaa acaaggagct ggaaaacctt gaaattaatc tgcgtaaaga
2281 aatcgcgaaa gcatttaaag gtaatgaagg atataaatct ctgtttaaaa aagacattat
2341 tgaaaccatc ctgcctgagt tcttggatga taaagatgaa attgccctgg ttaatagctt
2401 taatggcttt acgactgcat ttaccggctt ttttgataac cgtgaaaaca tgttcagcga
2461 agaagcgaaa tcgacttcca ttgccttccg ttgcattaat gaaaacctga cccgctacat
2521 ttctaatatg gacatctttg aaaaagttga tgccattttc gacaaacatg aagttcaaga
2581 aatcaaagaa aagattttaa actccgatta tgatgttgaa gatttctttg agggagaatt
2641 tttcaatttt gttctcaccc aagagggtat tgatgtctat aacgccatta tcggaggatt
2701 tgttacggaa tcaggcgaaa agattaaagg actgaatgaa tacattaatt tatacaatca
2761 gaaaaccaaa cagaaattgc caaaattcaa accactgtat aaacaggtat taagcgaccg
2821 tgaatccttg tccttttatg gcgaaggcta tacaagcgat gaagaggtgc tcgaagtatt
2881 ccgtaatact ttgaacaaaa actcggaaat cttctccagc attaaaaaac tggaaaaatt
2941 gtttaaaaat ttcgatgaat atagtagcgc aggcatcttc gtcaaaaatg gccctgcgat
3001 ttcaaccatt tctaaagaca ttttcggtga atggaacgtg atccgcgata aatggaacgc
3061 ggagtatgac gacattcatc tgaaaaaaaa agccgttgtt accgaaaagt atgaagatga
3121 ccgccgcaag agctttaaaa aaattggtag tttctcactg gaacagttac aagaatatgc
3181 agacgccgat ctgtcggttg tggaaaagtt aaaagagatt attatccaga aagtcgatga
3241 aatttataaa gtgtacggct ccagcgaaaa gctctttgat gccgactttg ttttagaaaa
3301 atcgctgaaa aaaaacgatg ccgtggtggc catcatgaaa gatctgttgg attcggtcaa
3361 atcgttcgaa aactatatca aggcgttctt tggcgaaggg aaagagacca atcgtgacga
3421 atcgttctat ggcgattttg tgttggcgta tgatattctt ctgaaagtag atcacatcta
3481 tgatgctatc cgcaactacg tgacccagaa accgtattcg aaggataaat ttaagctgta
3541 ttttcaaaac cctcagttta tgggtggctg ggataaagat aaagaaaccg actaccgcgc
3601 gacgatcttg cgctatggct ctaagtacta tttagccatc atggataaaa agtatgcaaa
3661 atgcctccag aaaatcgata aggatgatgt caacgggaac tacgaaaaaa tcaactacaa
3721 actcttgcct ggcccgaata aaatgctgcc gaaagtcttt ttttccaaaa aatggatggc
3781 ctattataac ccttccgaag atattcagaa gatttacaag aacggtacct ttaaaaaagg
3841 tgacatgttt aatctgaatg actgtcacaa gcttattgat ttcttcaaag atagtatttc
3901 tcgctaccct aaatggtcaa acgcgtacga ttttaatttc tcagaaacag agaaatacaa
3961 agatattgcg gggttttatc gcgaagtcga agaacaaggg tataaggtgt cgttcgaatc
4021 ggcttccaaa aaggaagtgg ataaacttgt tgaagaaggc aaactctata tgttccagat
4081 ctacaacaaa gatttctccg acaagagcca tggcacgccg aacttacata ccatgtactt
4141 taaattactg ttcgatgaaa ataatcatgg tcagattcgc ttgagcggtg gcgccgaact
4201 gtttatgcgc cgcgcatcat tgaaaaagga agaacttgtt gtgcacccag caaacagtcc
4261 gattgcaaac aaaaatccgg ataacccgaa gaagactacc accttaagct atgatgtata
4321 taaagataaa cgttttagcg aagatcagta tgaactccac attccgatcg cgattaacaa
4381 atgtcctaag aacatcttta aaatcaatac cgaggttcgt gtactgctca aacacgacga
4441 caacccctat gttattggca ttgaccgcgg cgaacgcaac ctgctgtata ttgtcgttgt
4501 ggatggcaaa ggtaacattg ttgaacagta tagccttaat gaaatcatca ataatttcaa
4561 cggcattcgt attaaaacgg actatcatag cctgctggac aaaaaagaga aagaacgctt
4621 cgaagcccgt cagaattgga cctctatcga aaatatcaag gaactgaaag ctgggtacat
4681 ttctcaagtg gtccacaaga tttgcgaact ggtggaaaag tatgacgcgg tgatcgcact
4741 ggaggatctg aattcgggct ttaaaaatag ccgtgtgaag gtggagaaac aggtttacca
4801 aaagttcgaa aaaatgctga ttgacaaact taattacatg gtggacaaaa aatctaatcc
4861 atgtgcgacc ggcggggctc tcaaaggcta tcagattacg aataaattcg aaagcttcaa
4921 atccatgtct acccagaacg gatttatctt ttacatcccc gcctggctga cttccaaaat
4981 tgatccgagt accggttttg ttaatctgct gaaaaccaaa tatacgtcga tcgccgattc
5041 aaaaaaattc attagcagct tcgaccgtat tatgtacgtc ccggaagaag acctgttcga
5101 attcgccctt gactataaaa atttctcgcg tacggatgcc gattatatca aaaaatggaa
5161 actgtacagc tacggcaacc gcatccgcat ttttcgcaac ccaaagaaga acaacgtatt
5221 tgactgggag gaagtttgct tgacgagcgc ttacaaagag ctctttaaca aatatgggat
5281 caattatcag caaggggaca tccgtgcgtt gctgtgtgag cagtcagata aagcgttcta
5341 cagctctttc atggctttaa tgtcgctgat gttgcagatg cgcaattcga tcactggccg
5401 caccgatgtg gatttcctga ttagcccggt caaaaatagc gatggcattt tttacgattc
5461 acgtaactat gaagcccagg aaaacgctat tctgccgaag aacgctgatg cgaacggcgc
5521 gtacaatatt gcccgtaagg tactgtgggc aattggtcag ttcaagaaag cggaagatga
5581 aaagttggat aaggtcaaaa ttgcgatctc gaataaagaa tggctggaat atgcgcagac
5641 cagcgtgaaa catgcctatc cctatgacgt gcccgattat gccagcctgg gcagcggctc
5701 ccccaagaaa aaacgcaagg tggaagatcc taagaaaaag cggaaagtgg actaaattgg
5761 tagtgggagc aacggcagca gcggatccgt gagcaagggc gaggaggata acatggccat
5821 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
5881 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
5941 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
6001 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
6061 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
6121 gacccaggac tcctccctcc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
6181 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
6241 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
6301 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
6361 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
6421 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
6481 cgagctgtac aaggggtcac ctaagaaaaa gcgaaaagtt gagtaataac attggaagtg
6541 gataacggat ccgcgatcgc ggcgcgccac ctggtggccg gccggtacca cgcgtgcgcg
6601 ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
6661 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
6721 gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata
6781 gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct
6841 ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag
6901 tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata
6961 accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg
7021 ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat
7081 taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt
7141 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
7201 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
7261 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataacatt gaaaaaggaa
7321 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
7381 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
7441 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
7501 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
7561 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
7621 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
7681 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
7741 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
7801 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
7861 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
7921 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
7981 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
8041 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
8101 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
8161 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
8221 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
8281 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
8341 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
8401 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
8461 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
8521 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
8581 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
8641 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
8701 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
8761 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
8821 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
8881 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
8941 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
9001 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
9061 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
9121 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
9181 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
9241 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
9301 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
9361 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
9421 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
9481 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
9541 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
9601 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
9661 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
9721 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
9781 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
9841 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
9901 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
9961 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
10021 gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg
10081 cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga
10141 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat
10201 tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
10261 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
10321 cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
10381 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
10441 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
10501 cgcgaacgcc agca
//
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