| 质粒名称: | pSMT3-M |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26589 |
| 存储实验室: | Tanya Parish |
|
载体骨架基本信息 |
|
| 载体名称: | pSMT3 |
| 载体出品公司: | -- |
| 载体抗性: | 潮霉素 |
| 空载体大小: | 5708 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 通过去除pAL5000复制子（A364）中364位的精氨酸残基构建的pSMT3的高拷贝数衍生物。 |
| 载体类型: | 分枝杆菌穿梭载体 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSMT3-M was a gift from Tanya Parish (Addgene plasmid # 26589 ; http://n2t.net/addgene:26589 ; RRID:Addgene_26589) |
| 参考文献部分: | Sensitive detection of gene expression in mycobacteria under replicating and non-replicating conditions using optimized far-red reporters. Carroll P, Schreuder LJ, Muwanguzi-Karugaba J, Wiles S, Robertson BD, Ripoll J, Ward TH, Bancroft GJ, Schaible UE, Parish T. PLoS One. 2010 . 5(3):e9823. 10.1371/journal.pone.0009823 PubMed 20352111 |
买家导航
pSMT3M载体基本信息
pSMT3M载体图谱质粒图谱和多克隆位点信息
pSMT3M载体简介
pSMT3M载体序列
LOCUS pSMT3-M 5708 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSMT3-M
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5708)
AUTHORS Carroll P, Schreuder LJ, Muwanguzi-Karugaba J, Wiles S, Robertson
BD, Ripoll J, Ward TH, Bancroft GJ, Schaible UE, Parish T
TITLE Sensitive detection of gene expression in mycobacteria under
replicating and non-replicating conditions using optimized far-red
reporters.
JOURNAL PLoS One. 2010 . 5(3):e9823.
PUBMED 20352111
REFERENCE 2 (bases 1 to 5708)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5708
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(436..452)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(437..453)
/label=pBluescriptKS
/note="For pBluescript vector"
rep_origin 4672..5260
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5161..5180
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5414..5431
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5548..5569
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5584..5614
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5622..5638
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5627..5649
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 5646..5662
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5646..5662
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
ORIGIN
1 ctagaggtga ccacaacgac gcgcccgctt tgatcgggga cgtctgcggc cgaccattta
61 cgggtcttgt tgtcgttggc ggtcatgggc cgaacatact cacccggatc ggagggccga
121 ggacaaggtc gaacgagggg catgacccgg tgcggggctt cttgcactcg gcataggcga
181 gtgctaagaa taacgttggc actcgcgacc ggtgagtcgt aggtcgggac ggtgaggcca
241 ggcccgtcgt cgcagcgagt ggcagcgagg acaacttgag ccgtccgtcg cgggcactgc
301 gcccggccag cgtaagtagc ggggttgccg tcacccggtg acccccggtt tcatccccga
361 tccggaggaa tcacttcgca atggccaaga caattgcgga tcccccgggc tgcaggaatt
421 cgatatcaag cttatcgata ccgtcgacct cgaggggggg cccggtacgt acccggggat
481 catcgagccg agaacgttat cgaagttggt catgtgtaat cccctcgttt gaactttgga
541 ttaagcgtag atacaccctt ggacaagcca gttggattcg gagacaagca aattcagcct
601 taaaaagggc gaggcctgcg gtggtggaac accgcagggc ctctaaccgc tcgacgcgct
661 gcaccaacca gcccgcgaac ggctggcagc cagcgtaagg cgcggctcat cgggcggcgt
721 tcgccacgat gtcctgcact tcgagccaag cctcgaacac ctgctggtgt gcacgactca
781 cccggttgtt gacaccgcgc gcggccgtgc gggctcggtg gggcggctgt gtcgcccttg
841 ccagcgtgag tagcgcgtac ctcacctcgc ccaacaggtc gcacacagcc gattcgtacg
901 ccataaagcc aggtgagccc accagctccg taagttcggg cgctgtgtgg ctcgtacccg
961 cgcattcagg cggcaggggg tctaacgggt ctaaggcggc gtgtacggcc gccacagcgg
1021 ctctcagcgg cccggaaacg tcctcgaaac gacgcatgtg ttcctcctgg ttggtacagg
1081 tggttggggg tgctcggctg tcgctggtgt tccaccacca gggctcgacg ggagagcggg
1141 ggagtgtgca gttgtggggt ggcccctcag cgaaatatct gacttggagc tcgtgtcgga
1201 ccatacaccg gtgattaatc gtggtctact accaagcgtg agccacgtcg ccgacgaatt
1261 tgagcagctc tggctgccgt actggccgct ggcaagcgac gatctgctcg aggggatcta
1321 ccgccaaagc cgcgcgtcgg ccctaggccg ccggtacatc gaggcgaacc caacagcgct
1381 ggcaaacctg ctggtcgtgg acgtagacca tccagacgca gcgctccgag cgctcagcgc
1441 ccgggggtcc catccgctgc ccaacgcgat cgtgggcaat cgcgccaacg gccacgcaca
1501 cgcagtgtgg gcactcaacg cccctgttcc acgcaccgaa tacgcgcggc gtaagccgct
1561 cgcatacatg gcggcgtgcg ccgaaggcct tcggcgcgcc gtcgacggcg accgcagtta
1621 ctcaggcctc atgaccaaaa accccggcca catcgcctgg gaaacggaat ggctccactc
1681 agatctctac acactcagcc acatcgaggc cgagctcggc gcgaacatgc caccgccgcg
1741 ctggcgtcag cagaccacgt acaaagcggc tccgacgccg ctagggcgga attgcgcact
1801 gttcgattcc gtcaggttgt gggcctatcg tcccgccctc atgcggatct acctgccgac
1861 ccggaacgtg gacggactcg gccgcgcgat ctatgccgag tgccacgcgc gaaacgccga
1921 attcccgtgc aacgacgtgt gtcccggacc gctaccggac agcgaggtcc gcgccatcgc
1981 caacagcatt tggcgttgga tcacaaccaa gtcgcgcatt tgggcggacg ggatcgtggt
2041 ctacgaggcc acactcagtg cgcgccagtc ggccatctcg cggaagggag cagcgcgcac
2101 ggcggcgagc acagttgcgc ggcgcgcaaa gtccgcgtca gccatggagg cattgctatg
2161 agcgacggct acagcgacgg ctacagcgac ggctacaacc ggcagccgac tgtccgcaaa
2221 aagcggcgcg tgaccgccgc cgaaggcgct cgaatcaccg gactatccga acgccacgtc
2281 gtccggctcg tggcgcagga acgcagcgag tggctcgccg agcaggctgc acgccgcgaa
2341 cgcatccgcg cctatcacga cgacgagggc cactcttggc cgcaaacggc caaacatttc
2401 gggctgcatc tggacaccgt taagcgactc ggctatcggg cgaggaaaga gcgtgcggca
2461 gaacaggaag cggctcaaaa ggcccacaac gaagccgaca atccaccgct gttctaacgc
2521 aattggggag cgggtgtcgc gggggttccg tggggggttc cgttgcaacg ggtcggacag
2581 gtaaaagtcc tggtagacgc tagttttctg gtttgggcca tgcctgtctc gttgcgtgtt
2641 tcgttgcgcc cgttttgaat accagccaga cgagacgggg ttctacgaat cttggtcgat
2701 accaagccat ttccgctgaa tatcggggag ctcaccgcca gaatcggtgg ttgtggtgat
2761 gtacgtggcg aactccgttg tagtgcctgt ggtggcatcc gtggccactc tcgttgcacg
2821 gttcgttgtg ccgttacagg ccccgttgac agctcaccga acgtagttaa aacatgctgg
2881 tcaaactagg tttaccaacg atacgagtca gctcatctag ggccagttct aggcgttgtt
2941 cgttgcgcgg ttcgttgcgc atgtttcgtg tggttgctag atggctccgc aaccacacgc
3001 ttcgaggttg agtgcttcca gcacgggcgc gatccagaag aacttcgtcg tgcgactgtc
3061 ctcgttgatc cttgccgagc tgggatggaa gctcggccga ccaccctgga ggagatgatc
3121 gaggatgcca gggcctttca cgcccgccgc tgctgagcgt ccgccgccgg gcccgcaccg
3181 ccgtcggccg gcccgctccg ggctcgcagc agcgggcttc ggcgcgggcc cggggctccc
3241 gagcgcgggc ggggctccgg gcggccgccg ggggccgggg gcggcgccgg gcggcccggg
3301 gcgtcaggcg ccgggggcgg tgtccggcgg cccccagagg aactgcgcca gttcctccgg
3361 atcggtgaag ccggagagat ccagcggggt ctcctcgaac acctcgaagt cgtgcaggaa
3421 ggtgaaggcg agcagttcgc gggcgaagtc ctcggtccgc ttccactgcg ccccgtcgag
3481 cagcgcggcc aggatctcgc ggtcgccccg gaaggcgttg agatgcagtt gcaccaggct
3541 gtagcgggag tctcccgcat agacgtcggt gaagtcgacg atcccggtga cctcggtcgc
3601 ggccaggtcc acgaagatgt tggtcccgtg caggtcgccg tggacgaacc ggggttcgcg
3661 gccggccagc agcgtgtcca cgtccggcag ccagtcctcc aggcggtcca gcagccgggg
3721 cgagaggtag ccccacccgc ggtggtcctc gacggtcgcc gcgcggcgtt cccgcagcag
3781 ttccgggaag acctcggaat ggggggtgag cacggtgttc ccggtcagcg gcaccctgtg
3841 cagccggccg agcacccggc cgagttcgcg ggccagggcg agcagcgcgt tccggtcggt
3901 cgtgccgtcc atcgcggacc gccaggtggt gccggtcatc cggctcatca ccaggtaggg
3961 ccacggccag gctccggtgc cgggccgcag ctcgccgcgg ccgaggaggc ggggcaccgg
4021 caccggggcg tccgccagga ccgcgtacgc ctccgactcc gacgcgaggc tctccggacc
4081 gcaccagtgc tcgccgaaca gcttgatcac cgggtcgggc tcgccgacca gtacggggtt
4141 ggtgctctcg ccgggcaccc gcagcaccgg cggcaccggc agcccgagct cctccagggc
4201 tcggcgggcc agcggctccc agaattcctg gtcgttccgc aggctcgcgt aggaatcatc
4261 cgaatcaata cggtcgagaa gtaacaggga ttcttgtgtc acagcggacc tctattcaca
4321 gggtacgggc cggcttaatt ccgcacggcc ggtcgcgaca cggcctgtcc gcaccgcgga
4381 tcaggcgttg acgatgacgg gctggtcggc cacgtcgggg acgacgggga gtcaggcaac
4441 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
4501 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
4561 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
4621 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
4681 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
4741 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
4801 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
4861 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
4921 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
4981 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
5041 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
5101 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
5161 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
5221 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
5281 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
5341 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
5401 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
5461 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
5521 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
5581 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
5641 tcacacagga aacagctatg accatgatta ccagatctgg ctcgagctcc accgcggtgg
5701 cggccgct
//
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