| 出品公司: | CAMBIA |
|---|---|
| 载体名称: | pCAMBIA3200 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 8581 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 氯霉素 |
| 筛选标记: | 草丁膦（磷丝菌素）phosphinothricin |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCAMBIA3200载体质粒基本信息
pCAMBIA3200质粒图谱载体图谱和pCAMBIA3200载体序列质粒序列多克隆位点信息
pCAMBIA3200质粒载体简介
pCAMBIA3200质粒序列载体序列
LOCUS pCAMBIA3200 8581 bp ds-DNA circular SYN 25-JUL-2016
DEFINITION Agrobacterium binary vector for plant transformation, with
bialophos/phosphinothricin resistance and chloramphenicol resistance
genes.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8581)
AUTHORS Cambia
TITLE Direct Submission
JOURNAL Exported Monday, July 25, 2016 from SnapGene Viewer 3.1.4 to Vector
NTI(R) format
http://www.snapgene.com
COMMENT VNTDATE|758505600|
COMMENT VNTDBDATE|776304000|
COMMENT VNTNAME|pCAMBIA3200|
COMMENT VNTAUTHORNAME|Cambia|
FEATURES Location/Qualifiers
CDS 1219..1848
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label=pVS1 StaA
CDS 2277..3350
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 3416..3610
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 3954..4094
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(4280..4868)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5139..5798)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
misc_feature 6326..6350
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 6428..6602
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(6609..7160)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase 草丁膦（磷丝菌素）
乙酰转移酶"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
promoter complement(7205..7882)
/label=35S promoter (enhanced)
/note="CaMV 35S promoter (enhanced)。cauliflower mosaic
virus 35S promoter with a duplicated enhancer region"
promoter 8109..8139
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8147..8163
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8171..8187
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 8183..8416
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
misc_feature 8197..8253
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(8257..8273)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 8476..8500
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
2281 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
2341 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
2401 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
2461 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
2521 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
2581 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
2641 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
2701 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
2761 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
2821 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
2881 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
2941 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
3001 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
3061 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
3121 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
3181 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
3241 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
3301 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
3361 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
3421 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
3481 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
3541 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
3601 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
3661 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
3721 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
3781 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
3841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
3901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
3961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
4021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
4081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
4141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
4201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
4261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
4321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
4381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
4441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
4501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
4561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
4621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
4681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
4741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
4801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
4861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
4921 cacgttaagg gattttggtc atgcatgata tatctcccaa tttgtgtagg gcttattatg
4981 cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag caattatgtg
5041 cttagtgcat ctaatcgctt gagttaacgc cggcgaagcg gcgtcggctt gaacgaattt
5101 ctagctagag gatcgcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca
5161 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
5221 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
5281 attgtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
5341 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
5401 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
5461 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
5521 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
5581 aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
5641 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
5701 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
5761 atatcaacgg tggtatatcc agtgattttt ttctccatga tgtttaactt tgttttaggg
5821 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
5881 taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acatgtcata
5941 acaagaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
6001 ggaccagttg cgtgacggca gttacgctac ttgcattaca gcttacgaac cgaacgaggc
6061 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
6121 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
6181 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
6241 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
6301 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
6361 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
6421 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
6481 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
6541 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
6601 aactcgagtc aaatctcggt gacgggcagg accggacggg gcggtaccgg caggctgaag
6661 tccagctgcc agaaacccac gtcatgccag ttcccgtgct tgaagccggc cgcccgcagc
6721 atgccgcggg gggcatatcc gagcgcctcg tgcatgcgca cgctcgggtc gttgggcagc
6781 ccgatgacag cgaccacgct cttgaagccc tgtgcctcca gggacttcag caggtgggtg
6841 tagagcgtgg agcccagtcc cgtccgctgg tggcgggggg agacgtacac ggtcgactcg
6901 gccgtccagt cgtaggcgtt gcgtgccttc caggggcccg cgtaggcgat gccggcgacc
6961 tcgccgtcca cctcggcgac gagccaggga tagcgctccc gcagacggac gaggtcgtcc
7021 gtccactcct gcggttcctg cggctcggta cggaagttga ccgtgcttgt ctcgatgtag
7081 tggttgacga tggtgcagac cgccggcatg tccgcctcgg tggcacggcg gatgtcggcc
7141 gggcgtcgtt ctgggctcat ggtagactcg agagagatag atttgtagag agagactggt
7201 gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga
7261 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
7321 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
7381 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
7441 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
7501 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
7561 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
7621 caccatgtta tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
7681 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
7741 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
7801 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
7861 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
7921 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
7981 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
8041 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
8101 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
8161 caatttcaca caggaaacag ctatgaccat gattacgaat tcgagctcgg tacccgggga
8221 tcctctagag tcgacctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg
8281 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
8341 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
8401 gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt
8461 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
8521 ttagaataac ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
8581 g
//
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