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pCMV-3Tag-1A载体质粒基本信息
pCMV-3Tag-1A质粒图谱载体图谱和pCMV-3Tag-1A载体序列质粒序列多克隆位点信息
pCMV-3Tag-1A质粒载体简介
pCMV-3Tag-1A质粒序列载体序列
LOCUS pCMV-3Tag-1A 4219 bp ds-DNA circular SYN 28-NOV-2013
DEFINITION Mammalian expression vector for tagging proteins with an N-terminal
3xFLAG epitope. For other reading frames, use pCMV-3Tag-1B or
pCMV-3Tag-1C.
ACCESSION .
VERSION .
KEYWORDS pCMV-3Tag-1A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4219)
AUTHORS Agilent Technologies
TITLE Direct Submission
JOURNAL Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4219
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 66..370
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 371..574
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 620..638
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 679..681
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 682..753
/codon_start=1
/product="three tandem FLAG epitope tags"
/note="3xFLAG"
/translation="DYKDDDDKDYKDDDDKDYKDDDDK"
misc_feature 754..828
/note="MCS"
/note="multiple cloning site"
misc_feature 836..846
/note="stop codons"
/note="stop codons in all three reading frames"
promoter complement(874..892)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 927..1134
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(1141..1596)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1623..1725
/gene="bla"
/note="AmpR promoter"
promoter 1729..2086
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 2121..2915
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 3147..3194
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 3523..4111
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataaggacta taaggacgat
721 gatgacaagg actacaaaga tgatgacgat aaagcccggg cgggatcccc cgggctgcag
781 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccttaatt
841 aattaaggta ccaggtaagt gtacccaatt cgccctatag tgagtcgtat tacaattcac
901 tcgatcggct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
961 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
1021 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
1081 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggaacgcgt
1141 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
1201 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
1261 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
1321 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
1381 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
1441 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
1501 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
1561 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
1621 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
1681 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagaatcct gaggcggaaa
1741 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
1801 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
1861 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
1921 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
1981 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
2041 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
2101 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
2161 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
2221 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
2281 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
2341 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
2401 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
2461 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
2521 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
2581 atcaggatga tctggacgaa gaacatcagg ggctcgcgcc agccgaactg ttcgccaggc
2641 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
2701 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
2761 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gaacttggcg
2821 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
2881 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
2941 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
3001 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
3061 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
3121 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
3181 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
3241 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
3301 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
3361 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
3421 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
3481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
3541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
3601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
3661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
3721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
3781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
3841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
3901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
3961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
4021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
4081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
4141 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
4201 ggataaccgt attaccgcc
//
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pCMV-3Tag-1A质粒载体应用举例
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