| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 141367 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-nsp1 |
| 物种来源: | SARS-CoV-2 |
| 插入大小: | -- |
| 基因突变: | human codon optimized |
| GenBank编号 | -- |
| 启动子 | EF1alpha |
| 标签/融合蛋白: | 2xStrep（插入件上的C端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141367 ; http://n2t.net/addgene:141367 ; RRID:Addgene_141367) |
| 参考文献部分: | A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug Repurposing. Gordon DE, et al.. BioRxiv March 2020 10.1101/2020.03.22.002386 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro 9453 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9453)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9453)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9453
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1182
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 231..1173
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1130..1150
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 1207..1216
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1771..1794
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 1831..1854
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 1866..2439
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(2033..2050)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 2260..2279
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 2472..3071
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2472..2491)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2968..2988
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 3085..3673
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3138..3158)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3556..3567)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3880..4513
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4641..4657)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4641..4657)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4654..4676)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4665..4681
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4689..4719)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4734..4755
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4872..4889)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5043..5631)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5123..5142)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5802..6662)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6425..6444
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6663..6767)
/gene="bla"
/label=AmpR promoter
polyA_signal 6815..6949
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6852..6871)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6906..6925
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 7117..7750
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 7797..7922
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8419..8652
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8837..8881
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 9144..9259
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
ORIGIN
1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
1201 ttcgccgcca ccatggagag tcttgtaccc ggctttaacg agaaaacaca tgttcaattg
1261 tcattgcctg tgctgcaagt ccgggacgta ctggttaggg ggttcggaga ttcagtagaa
1321 gaggtgctgt ctgaggcgag gcaacatttg aaagacggga cgtgcgggct ggttgaggtg
1381 gaaaagggag tacttccaca gttggaacaa ccgtatgttt ttattaagcg gagtgatgcc
1441 aggactgcgc cgcacggtca tgtcatggtt gagcttgtcg cagagcttga aggcattcaa
1501 tatgggcgct ccggtgaaac tcttggagta cttgtgccgc acgtaggcga aattccagtg
1561 gcatatcgga aggtgctgtt gagaaaaaat gggaacaagg gcgcaggggg ccacagttat
1621 ggagccgacc tcaaatcctt tgaccttggc gacgagttgg ggacagatcc ctacgaggat
1681 ttccaggaaa actggaatac gaaacatagc agtggcgtta ctcgcgagtt gatgcgcgaa
1741 ctgaacggcg gcctcgaagg cggcggggga tggagccatc cacaattcga gaaaggcggt
1801 ggttcaggag gaggtagcgg gggtggatca tggtcacatc cgcagtttga aaagtaagga
1861 tcccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
1921 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
1981 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
2041 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
2101 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
2161 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
2221 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
2281 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
2341 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
2401 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga
2461 gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
2521 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
2581 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
2641 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
2701 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
2761 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
2821 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
2881 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
2941 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
3001 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
3061 ccggtgccta gacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
3121 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
3181 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
3241 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
3301 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
3361 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
3421 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
3481 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
3541 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
3601 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
3661 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
3721 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
3781 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
3841 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
3901 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
3961 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
4021 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
4081 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
4141 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
4201 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
4261 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
4321 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
4381 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
4441 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
4501 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
4561 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
4621 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
4681 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
4741 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
4801 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
4861 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
4921 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
4981 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
5041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
5101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
5161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
5221 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
5281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
5341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
5401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
5461 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
5521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
5581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
5641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
5701 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
5761 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
5821 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
5881 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
5941 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
6001 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
6061 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
6121 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
6181 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
6241 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
6301 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
6361 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
6421 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
6481 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
6541 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
6601 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
6661 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
6721 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
6781 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
6841 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
6901 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
6961 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
7021 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
7081 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
7141 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
7201 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
7261 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
7321 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
7381 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
7441 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
7501 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
7561 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
7621 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
7681 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
7741 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
7801 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
7861 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
7921 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
7981 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
8041 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
8101 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
8161 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
8221 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
8281 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
8341 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
8401 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
8461 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
8521 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
8581 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
8641 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
8701 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
8761 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
8821 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
8881 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
8941 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
9001 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
9061 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
9121 gaacggatct cgacggtatc gcctttaaaa gaaaaggggg gattgggggg tacagtgcag
9181 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
9241 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttatcgat
9301 gagtaattca tacaaaagga ctcgcccctg ccttggggaa tcccagggac cgtcgttaaa
9361 ctcccactaa cgtagaaccc agagatcgct gcgttcccgc cccctcaccc gcccgctctc
9421 gtcatcactg aggtggagaa gagcatgcgt gag
//
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