| 出品公司: | ATCC |
|---|---|
| 载体名称: | YEplac181 |
| 质粒类型: | 酿酒酵母表达载体；2μ质粒型载体；酵母附加体型载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lacZ |
| 载体大小: | 5741 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | LEU2 |
| 表达菌株: | 酿酒酵母、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | 87588 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
YEplac181载体质粒基本信息
YEplac181质粒图谱载体图谱和YEplac181载体序列质粒序列多克隆位点信息
YEplac181质粒载体简介
YEplac181质粒序列载体序列
LOCUS YEplac181 5741 bp DNA SYN
DEFINITION YEplac181
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5741
/organism="YEplac181"
/mol_type="other DNA"
promoter 142..171
/label="lac_promoter"
misc_feature 185..207
/label="M13_pUC_rev_primer"
promoter 206..224
/label="M13_reverse_primer"
misc_feature 287..442
/label="lacZ_a"
promoter complement(290..306)
/label="M13_forward20_primer"
misc_feature complement(299..321)
/label="M13_pUC_fwd_primer"
rep_origin complement(743..1907)
/label="2micron2_origin"
gene complement(2227..3319)
/label="LEU2 (variant)"
/gene="LEU2 (variant)"
CDS complement(2227..2805)
/label="ORF frame 3"
/translation="MAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPT
LKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASL
PDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKV
LDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
promoter 3870..3898
/label="AmpR_promoter"
/translation="MAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPT
LKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASL
PDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKV
LDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
gene 3940..4800
/label="Ampicillin"
/gene="Ampicillin"
/translation="MAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPT
LKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASL
PDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKV
LDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
CDS 3940..4800
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 4955..5574
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca
541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
2101 aagctgtcaa acatgagaat taattctacc ctatgaacat attccatttt gtaatttcgt
2161 gtcgtttcta ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga
2221 atctttttaa gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt
2281 actgttggaa ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc
2341 atcttcaatg gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc
2401 agacaagata gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccgtg
2461 gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga
2521 tggcaacaaa cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa
2581 catgttgctg gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc
2641 ggcagaatca atcaattgat gttgaacctt caatgtagga aattcgttct tgatggtttc
2701 ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca
2761 aataggcaat ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg
2821 cacttctgga acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt
2881 tctcttacca aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt
2941 agcaaattgt ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg
3001 tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct
3061 aacactacct gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt
3121 cttggaggct tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc
3181 accaattaaa tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt
3241 aagaacctta atggcttcgg ctgtgatctt gaccaacgtg gtcacctggc aaaacgacga
3301 tcttcttagg ggcagacata ggggcagaca ttagaatgta tatccttgaa atatatatat
3361 atattgctga aatgtaaaag gtaagaaaag ttagaataag acgattgcta accacctatt
3421 ggaaaaaaca ataggtcctt aaataatatt gtcaacttca agtattgtga tgcaagcatt
3481 tagtcatgaa cgcttctcta ttctatatga aaagccggtt ccggcctctc acctttcctt
3541 tttctcccaa tttttcagtt gaaaaaggta tatgcgcggc gacctctgaa attaacaaaa
3601 aatttccagt catcgaattt gtctgtgcga tagcgcccct gtgtgttctc gttatgttga
3661 ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata cctgagtatt
3721 cccacagtta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
3781 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
3841 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
3901 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
3961 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
4021 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
4081 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
4141 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
4201 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
4261 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
4321 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
4381 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
4441 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
4501 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
4561 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
4621 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
4681 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
4741 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
4801 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
4861 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
4921 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
4981 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
5041 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
5101 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
5161 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
5221 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
5281 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
5341 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
5401 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
5461 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
5521 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
5581 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
5641 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
5701 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag a
//
