| 出品公司: | ATCC |
|---|---|
| 载体名称: | YEplac195 |
| 质粒类型: | 酿酒酵母表达载体；2μ质粒型载体；酵母附加体型载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lacZ |
| 载体大小: | 5241 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | URA3 |
| 表达菌株: | 酿酒酵母、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | #87589 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
YEplac195载体质粒基本信息
YEplac195质粒图谱载体图谱和YEplac195载体序列质粒序列多克隆位点信息
YEplac195质粒载体简介
YEplac195质粒序列载体序列
LOCUS YEplac195 5241 bp DNA SYN
DEFINITION YEplac195
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5241
/organism="YEplac195"
/mol_type="other DNA"
promoter 142..171
/label="lac_promoter"
misc_feature 185..207
/label="M13_pUC_rev_primer"
promoter 206..224
/label="M13_reverse_primer"
misc_feature 287..442
/label="lacZ_a"
promoter complement(290..306)
/label="M13_forward20_primer"
misc_feature complement(299..321)
/label="M13_pUC_fwd_primer"
rep_origin complement(743..1907)
/label="2micron2_origin"
CDS complement(2200..3003)
/label="ORF frame 1"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
gene complement(2203..3003)
/label="URA3"
/gene="URA3"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
CDS 2492..2968
/label="ORF frame 2"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
promoter 3370..3398
/label="AmpR_promoter"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
gene 3440..4300
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
CDS 3440..4300
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 4455..5074
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca
541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
2101 aagctgtcaa acatgagaat tgggtaataa ctgatataat taaattgaag ctctaatttg
2161 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
2221 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
2281 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
2341 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
2401 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
2461 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
2521 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
2581 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
2641 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
2701 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
2761 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
2821 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
2881 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
2941 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
3001 catgatttat cttcgtttcc tgcatgtttt tgttctgtgc agttgggtta agaatactgg
3061 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
3121 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg
3181 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagcta attcttgaag
3241 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
3301 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
3361 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
3421 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
3481 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
3541 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
3601 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
3661 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
3721 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
3781 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
3841 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
3901 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
3961 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
4021 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
4081 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
4141 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
4201 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
4261 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
4321 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
4381 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
4441 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
4501 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
4561 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
4621 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
4681 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
4741 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
4801 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
4861 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
4921 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
4981 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
5041 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
5101 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
5161 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
5221 agtgagcgag gaagcggaag a
//
