| 出品公司: | ATCC |
|---|---|
| 载体名称: | YIplac128 |
| 质粒类型: | 酿酒酵母表达载体；酵母整合型载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lacZ |
| 载体大小: | 4302 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | LEU2 |
| 表达菌株: | 酿酒酵母、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | #87592 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
YIplac128载体质粒基本信息
YIplac128质粒图谱载体图谱和YIplac128载体序列质粒序列多克隆位点信息
YIplac128质粒载体简介
YIplac128质粒序列载体序列
LOCUS YIplac128 4302 bp DNA SYN
DEFINITION YIplac128
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4302
/organism="YIplac128"
/mol_type="other DNA"
promoter 142..171
/label="lac_promoter"
misc_feature 185..207
/label="M13_pUC_rev_primer"
promoter 206..224
/label="M13_reverse_primer"
misc_feature 287..442
/label="lacZ_a"
promoter complement(290..306)
/label="M13_forward20_primer"
misc_feature complement(299..321)
/label="M13_pUC_fwd_primer"
misc_feature 634..656
/label="pGEX_3_primer"
gene complement(797..1891)
/label="LEU2 (variant)"
/gene="LEU2 (variant)"
CDS complement(797..1903)
/label="ORF frame 3"
/translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
promoter 2431..2459
/label="AmpR_promoter"
/translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
gene 2501..3361
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSAPMSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFD
FENHLIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRK
ELQLYANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVA
WDSEQYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEF
PTLKVQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLA
SLPDKNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVK
KVLDAGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
CDS 2501..3361
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 3516..4135
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
661 caccgtcatc accgaaacgc gcgagacgaa agggcctacc ctatgaacat attccatttt
721 gtaatttcgt gtcgtttcta ttatgaattt catttataaa gtttatgtac aaatatcata
781 aaaaaagaga atctttttaa gcaaggattt tcttaacttc ttcggcgaca gcatcaccga
841 cttcggtggt actgttggaa ccacctaaat caccagttct gatacctgca tccaaaacct
901 ttttaactgc atcttcaatg gccttacctt cttcaggcaa gttcaatgac aatttcaaca
961 tcattgcagc agacaagata gtggcgatag ggtcaacctt attctttggc aaatctggag
1021 cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca
1081 aggacgcaga tggcaacaaa cccaaggaac ctgggataac ggaggcttca tcggagatga
1141 tatcaccaaa catgttgctg gtgattataa taccatttag gtgggttggg ttcttaacta
1201 ggatcatggc ggcagaatca atcaattgat gttgaacctt caatgtagga aattcgttct
1261 tgatggtttc ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat
1321 ccaaggacca aataggcaat ggtggctcat gttgtagggc catgaaagcg gccattcttg
1381 tgattctttg cacttctgga acggtgtatt gttcactatc ccaagcgaca ccatcaccat
1441 cgtcttcctt tctcttacca aagtaaatac ctcccactaa ttctctgaca acaacgaagt
1501 cagtaccttt agcaaattgt ggcttgattg gagataagtc taaaagagag tcggatgcaa
1561 agttacatgg tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt
1621 gttcaggtct aacactacct gtaccccatt taggaccacc cacagcacct aacaaaacgg
1681 catcaacctt cttggaggct tccagcgcct catctggaag tgggacacct gtagcatcga
1741 tagcagcacc accaattaaa tgattttcga aatcgaactt gacattggaa cgaacatcag
1801 aaatagcttt aagaacctta atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg
1861 gcaaaacgac gatcttctta ggggcagaca taggggcaga cattagaatg gtatatcctt
1921 gaaatatata tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat
1981 tgctaaccac ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat
2041 tgtgatgcaa gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc
2101 ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc gtcaggcgac
2161 ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat agcgcccctg
2221 tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga actcttgcat
2281 cttacgatac ctgagtattc ccacagttgg cctcgtgata cgcctatttt tataggttaa
2341 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
2401 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
2461 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
2521 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
2581 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
2641 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
2701 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
2761 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
2821 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
2881 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
2941 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
3001 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
3061 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
3121 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
3181 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
3241 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
3301 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
3361 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
3421 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
3481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
3541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
3601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
3661 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
3721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
3781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
3841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
3901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
3961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
4021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
4081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
4141 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
4201 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
4261 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//
