| 出品公司: | ATCC |
|---|---|
| 载体名称: | YIplac204 |
| 质粒类型: | 酿酒酵母表达载体；酵母整合型载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lacZ |
| 载体大小: | 3545 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | TRP1 |
| 表达菌株: | 酿酒酵母、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | #87591 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
YIplac204载体质粒基本信息
YIplac204质粒图谱载体图谱和YIplac204载体序列质粒序列多克隆位点信息
YIplac204质粒载体简介
YIplac204质粒序列载体序列
LOCUS YIplac204 3545 bp DNA SYN
DEFINITION YIplac204
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3545
/organism="YIplac204"
/mol_type="other DNA"
promoter 142..171
/label="lac_promoter"
misc_feature 185..207
/label="M13_pUC_rev_primer"
promoter 206..224
/label="M13_reverse_primer"
promoter complement(290..306)
/label="M13_forward20_primer"
misc_feature complement(299..321)
/label="M13_pUC_fwd_primer"
misc_feature 634..656
/label="pGEX_3_primer"
gene complement(775..1449)
/label="TRP1"
/gene="TRP1"
CDS complement(775..1449)
/label="ORF frame 3"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
promoter 1674..1702
/label="AmpR_promoter"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
gene 1744..2604
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
CDS 1744..2604
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 2759..3378
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
661 caccgtcatc accgaaacgc gcgagacgaa agggcgatct tttatgcttg cttttcaaaa
721 ggcttgcagg caagtgcaca aacaatactt aaataaatac tactcagtaa taacctattt
781 cttagcattt ttgacgaaat ttgctatttt gttagagtct tttacaccat ttgtctccac
841 acctccgctt acatcaacac caataacgcc atttaatcta agcgcatcac caacattttc
901 tggcgtcagt ccaccagcta acataaaatg taagctctcg gggctctctt gccttccaac
961 ccagtcagaa atcgagttcc aatccaaaag ttcacctgtc ccacctgctt ctgaatcaaa
1021 caagggaata aacgaatgag gtttctgtga agctgcactg agtagtatgt tgcagtcttt
1081 tggaaatacg agtcttttaa taactggcaa accgaggaac tcttggtatt cttgccacga
1141 ctcatctcca tgcagttgga cgatatcaat gccgtaatca ttgaccagag ccaaaacatc
1201 ctccttaggt tgattacgaa acacgccaac caagtatttc ggagtgcctg aactattttt
1261 atatgctttt acaagacttg aaattttcct tgcaataacc gggtcaattg ttctctttct
1321 attgggcaca catataatac ccagcaagtc agcatcggaa tctagtgcac attctgcggc
1381 ctctgtgctc tgcaagccgc aaactttcac caatggacca gaactacctg tgaaattaat
1441 aacagacata ctccaagctg cctttgtgtg cttaatcacg tatactcacg tgctcaatag
1501 tcaccaatgc cctccctctt ggccctctcc ttttcttttt tcgaccgaat tggcctcgtg
1561 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
1621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
1681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
1741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
1801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
1861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
1921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
1981 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
2041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
2101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
2161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
2221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
2281 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
2341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
2401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
2461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
2521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
2581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
2641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
2701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
2761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
2821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
2881 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
2941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
3001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
3061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
3121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
3181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
3241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
3301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
3361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
3421 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
3481 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
3541 gaaga
//
