| 出品公司: | ATCC |
|---|---|
| 载体名称: | YIplac211 |
| 质粒类型: | 酿酒酵母表达载体；酵母整合型载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lacZ |
| 载体大小: | 3797 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | URA3 |
| 表达菌株: | 酿酒酵母、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | #87591 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
YIplac211载体质粒基本信息
YIplac211质粒图谱载体图谱和YIplac211载体序列质粒序列多克隆位点信息
YIplac211质粒载体简介
YIplac211质粒序列载体序列
LOCUS YIplac211 3797 bp DNA SYN
DEFINITION YIplac211
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3797
/organism="YIplac211"
/mol_type="other DNA"
promoter 142..171
/label="lac_promoter"
misc_feature 185..207
/label="M13_pUC_rev_primer"
promoter 206..224
/label="M13_reverse_primer"
promoter complement(290..306)
/label="M13_forward20_primer"
misc_feature complement(299..321)
/label="M13_pUC_fwd_primer"
misc_feature 634..656
/label="pGEX_3_primer"
CDS complement(774..1577)
/label="ORF frame 1"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
gene complement(777..1577)
/label="URA3"
/gene="URA3"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
CDS 1066..1542
/label="ORF frame 1"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
promoter 1926..1954
/label="AmpR_promoter"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
gene 1996..2856
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
CDS 1996..2856
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 3011..3630
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
661 caccgtcatc accgaaacgc gcgagacgaa agggcgggta ataactgata taattaaatt
721 gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt
781 tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct
841 accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct
901 gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct
961 aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct
1021 ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc
1081 ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg
1141 cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg
1201 cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca
1261 gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa
1321 aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca
1381 gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac
1441 tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg
1501 tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta
1561 tatgtagctt tcgacatgat ttatcttcgt ttcctgcatg tttttgttct gtgcagttgg
1621 gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat
1681 ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa
1741 tttcaaagaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaattgaaaa
1801 gctggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
1861 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
1921 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
1981 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
2041 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
2101 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
2161 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
2221 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
2281 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
2341 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
2401 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
2461 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
2521 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
2581 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
2641 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
2701 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
2761 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
2821 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
2881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
2941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
3001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
3061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
3121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
3181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
3241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
3301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
3361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
3421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
3481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
3541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
3601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
3661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
3721 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
3781 agcgaggaag cggaaga
//
