| 质粒名称: | V5-GFP-TurboID-KDEL_pLX208 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 202020 |
| 存储实验室: | Alice Ting |
|
载体骨架基本信息 |
|
| 载体名称: | pLX208 |
| 完整质粒大小: | 10080 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Hygromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | V5-TurboID-KDEL |
| 物种来源: | Synthetic |
| 插入大小: | 1851 bp |
| 启动子: | CMV |
| 标签/融合蛋白: |
V5 (N terminal on insert)
GFP (N terminal on insert)
|
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | CMV-F |
|
文献引用方法 |
|
| 材料和方法部分: | V5-GFP-TurboID-KDEL_pLX208 was a gift from Alice Ting (Addgene plasmid # 202020 ; http://n2t.net/addgene:202020 ; RRID:Addgene_202020) |
| 参考文献部分: | Dynamic mapping of proteome trafficking within and between living cells by TransitID. Qin W, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor JP, Udeshi ND, Carr SA, Ting AY. Cell. 2023 Jun 23:S0092-8674(23)00596-2. doi: 10.1016/j.cell.2023.05.044. |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS V5-GFP-TurboID-KDEL_pLX208 10080 bp ds-DNA circular SYN 23-JUN-2023
DEFINITION expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector.
ACCESSION .
VERSION .
KEYWORDS V5-GFP-TurboID-KDEL_pLX208
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10080)
AUTHORS Xu WQ, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor
JP, Udeshi ND, Carr SA, Ting AY
TITLE Dynamic mapping of proteome trafficking within and between living
cells by TransitID.
JOURNAL bioRxiv. 2023 Feb 8:2023.02.07.527548. doi:
10.1101/2023.02.07.527548. Preprint.
PUBMED 36798302
REFERENCE 2 (bases 1 to 10080)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 23, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10080
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1..589
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 490..509
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 743..760
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 877..898
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 913..943
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 951..967
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 956..978
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 975..991
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 975..991
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 1010..1030
/label=T3
/note="T3 promoter, forward primer"
promoter 1012..1030
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 1058..1284
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 1285..1465
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1512..1637
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 2130..2363
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2548..2592
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 2767..3267
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 3186..3204
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 3279..4298
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
"
misc_feature 4355..4472
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 4588..4891
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4892..5095
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 5046..5066
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
sig_peptide 5121..5183
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
CDS 5184..5225
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 5232..5948
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 5232..5948
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5277..5298)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5538..5557)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5885..5906
/label=EGFP-C
/note="EGFP, forward primer"
CDS 5967..6923
/codon_start=1
/product="mutant of the E. coli biotin ligase BioID
engineered for improved catalytic efficiency (Branon et
al., 2018)"
/label=TurboID
/translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
misc_feature 6990..7578
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7043..7063)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7461..7472)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7650..7883
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7961..8082
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7998..8017)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8052..8071
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 8122..8257
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8184..8203
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(8277..8296)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8278..8296)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8306..8323)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8306..8322)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8315..8337)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 8464..8919
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8551..8570)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8761..8782
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 8945..9049
/gene="bla"
/label=AmpR promoter
CDS 9050..9910
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9268..9287)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
541 tgagcgtcga tttttgtgat gctcgtcagg ggggctgagc ctatggaaaa acgccagcaa
601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
3241 gaccgaatca ccgacctctc tccccagggg gtaccaccat gaaaaagcct gaactcaccg
3301 ctacctctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
3361 tctccgaggg cgaagaatct cgggctttca gcttcgatgt gggagggcgt ggatatgtcc
3421 tgcgggtgaa tagctgcgcc gatggtttct acaaagatcg ctatgtttat cggcactttg
3481 catccgccgc tctccctatt cccgaagtgc ttgacattgg ggagttcagc gagagcctga
3541 cctattgcat ctcccgccgt gcacagggtg tcaccttgca agacctgcct gaaaccgaac
3601 tgcccgctgt tctccagccc gtcgccgagg ccatggatgc catcgctgcc gccgatctta
3661 gccagaccag cgggttcggc ccattcggac ctcaaggaat cggtcaatac actacatggc
3721 gcgatttcat ctgcgctatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
3781 acaccgtcag tgcctccgtc gcccaggctc tcgatgagct gatgctttgg gccgaggact
3841 gccccgaagt ccggcacctc gtgcacgccg atttcggctc caacaatgtc ctgaccgaca
3901 atggccgcat aacagccgtc attgactgga gcgaggccat gttcggggat tcccaatacg
3961 aggtcgccaa catcttcttc tggaggccct ggttggcttg tatggagcag cagacccgct
4021 acttcgagcg gaggcatccc gagcttgcag gatctcctcg gctccgggct tatatgctcc
4081 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
4141 gggctcaggg tcgctgcgac gcaatcgtcc ggtccggagc cgggactgtc gggcgtacac
4201 aaatcgcccg cagaagcgct gccgtctgga ccgatggctg tgtggaagtg ctcgccgata
4261 gtggaaacag acgccccagc actcgtccta gggcaaagga tctgcagtaa tgagaattct
4321 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
4381 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
4441 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
4501 cactttggcg ccggctcagg gggcccgggg ttattaatag taatcaatta cggggtcatt
4561 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
4621 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
4681 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
4741 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
4801 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
4861 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
4921 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
4981 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
5041 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
5101 gctaactgtc gggatccacc atggagacag acacactcct gctatgggta ctgctgctct
5161 gggttccagg ttccactggt gacggcaagc ccatccccaa ccccctgctg ggcctggaca
5221 gcaccggatc catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
5281 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
5341 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
5401 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
5461 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
5521 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
5581 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
5641 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
5701 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
5761 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
5821 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
5881 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
5941 tgtacaaggg gggaggcggg ggtagtaaag acaatactgt gcctctgaag ctgatcgctc
6001 tcctggctaa tggcgagttc catagtggcg aacagctggg agaaaccctg ggcatgtcca
6061 gggccgctat caacaagcac attcagactc tgcgcgactg gggcgtggac gtgttcaccg
6121 tgcccggaaa gggctactct ctgcccgagc ctatcccgct gctgaacgct aaacagattc
6181 tgggacagct ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt
6241 acctgctgga tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc
6301 aggcagggag aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc
6361 tgagtatgtt ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg
6421 gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc
6481 ccaatgacct gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa
6541 taacaggcga tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg
6601 tggaggaaag cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg
6661 acaggaatac tctggccgct acgctgatcc gagagctgcg ggcagccctg gaactgttcg
6721 agcaggaagg cctggctcca tatctgccac ggtgggagaa gctggataac ttcatcaata
6781 gacccgtgaa gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata
6841 aacagggagc cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa
6901 tcagtctgcg gtctgccgaa aagggtggag gcgggtctgg aggcgggggt agtaccggta
6961 aggacgagct gtaaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
7021 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
7081 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
7141 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
7201 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
7261 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
7321 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
7381 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
7441 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
7501 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
7561 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
7621 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
7681 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
7741 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
7801 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
7861 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
7921 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
7981 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
8041 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
8101 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
8161 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
8221 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
8281 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
8341 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
8401 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
8461 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
8521 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
8581 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
8641 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
8701 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
8761 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
8821 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
8881 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
8941 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
9001 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
9061 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
9121 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
9181 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
9241 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
9301 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
9361 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
9421 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
9481 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
9541 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
9601 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
9661 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
9721 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
9781 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
9841 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
9901 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
9961 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
10021 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
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