| 质粒名称: | pBR322-PVCpnf17-22 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 198272 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322 |
| 完整质粒大小: | 9096 bp |
| 载体类型: | Bacterial Expression |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PVCpnf17-22 |
| 物种来源: | Photorhabdus asymbiotica |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | 未知(未破坏) |
| 3’克隆位点: | 未知 (未破坏) |
|
文献引用方法 |
|
| 材料和方法部分: | pBR322-PVCpnf17-22 was a gift from Feng Zhang (Addgene plasmid # 198272 ; http://n2t.net/addgene:198272 ; RRID:Addgene_198272) |
| 参考文献部分: | Programmable protein delivery with a bacterial contractile injection system. Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. 10.1038/s41586-023-05870-7 PubMed 36991127 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBR322-PVCpnf17-22 9096 bp ds-DNA circular SYN 23-MAR-2023
DEFINITION Unmodified pPayload; contains WT PVC payloads/regulatory genes.
ACCESSION .
VERSION .
KEYWORDS pBR322-PVCpnf17-22
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9096)
AUTHORS Kreitz J, Zhang F
TITLE Programmable protein delivery with a bacterial contractile injection
system
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9096)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 23, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9096
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 3052..3243
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind complement(3237..3259)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3345..3485
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 3400..3419
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(3500..3517)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3671..4259)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3751..3770)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4430..5290)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5053..5072
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5291..5395)
/gene="bla"
/label=AmpR promoter
primer_bind 5463..5481
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5508..5536
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
ORIGIN
1 atttttggcg gatcacaaaa atagggctga gtcccttcaa gtgagttttg aaatatacaa
61 ttataccctt catcctttaa gttgttgctt tgttggctgt tttcattcac ctcagtcaca
121 tagttatcta tgctcctgag gattcgttca cttgccgtcg tgctgcaact cgaaatctat
181 tgggtatata tgtaattttc atattctgtt tcattttaac ggtattattt aaatatttat
241 atttagaaca ttaggttatt agaatagata attttaggta aatgtaatta attcaaccat
301 cacggagagt ttatcaatgt taaaatatgc taatcctcag accgtagcca cacaacgtac
361 taaaaatact gcgaagaaac cgccatcatc aacctctttt gatgggcacc ttgaactttc
421 aaatggtgaa aaccagcctt acgaaggcca taagattagg aaaatcaaag gattgagaca
481 acatcttgcg gataggagtc ttaataaagg gcatatttcc ccgttgatga ataagggatt
541 attagtaggt agtaaggatg tgtcaattga catcccagtt atagctcatc gttatgacag
601 cagccatcaa cttacagatg cggagccttt gaaagctgat agtcactcca atcatctaga
661 ccctttttat ggtgttattg caggatttcg tggtgatcag gtgacatcat cagagtcggg
721 ttcaggatca ataggcgtac attgggggaa aaatacgctg gattccaata ttatgggtgt
781 taatgtggtt aatggtgcat cgggaaccgt gggaataaga attgctttga aggatattca
841 gcatgggtcc ccggttattg tcacgtctgg agcattgagc ggttgtacta tggtatattc
901 ggtgaaaaat ggctattttt ttgcctatca tacggggcag aaaccgggta ataatgaatg
961 gaaaacaggc aggcagggtg ttgttgctac ctatctgagc catcaagccc tttcgccgga
1021 tagtgaacct atgacggttg gtgaacagaa taacgattta gtgaatattt ttgcaaatta
1081 tgatcagagt gttattacct atatgggaaa accgggagtg ctgattgata aaatggcaga
1141 aaatgttggt gtttttaatt atgacgaaat taagcctgaa aaacctgcta ttagagctgg
1201 ctacagttat gcgttgctgg ctaaggacga taaaggaaag gtgaacgtta aggtactatc
1261 ggaagatgtt atcgtttcat caggtaagca gggaaatact gttaaggcca ttaattcact
1321 taaaaaacgg ttgttataat tttgcccaat gagggcagga ggtacggatt ttcattagca
1381 cttatcgaag cggatatatg tttgtgacta cattgtcaga aaatttcatt catcaacttg
1441 aattagaaca gtgtccatat aacttattta gaataaagga aggaaattaa tcttagatac
1501 aatgataagg gaaataaaat agaaagtaga gttaattgta ttaataaggt gctggagctt
1561 tacttacaaa atatcaatga tttttggttt cgtataagta attaatttag agtaatgaaa
1621 aattatttgt gggttaaatt ttatttattg aaaattcatt ggtttatttt atgaaatagg
1681 aaggtaatag cggtattatt cggttgaaat aaccgtatag ataaatagta ggatttagat
1741 aaattatttc atatatgaca atttttacta ttatatgggg tttgtaagtc gaccgatgcc
1801 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
1861 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
1921 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
1981 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
2041 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
2101 gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
2161 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
2221 gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct
2281 gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt
2341 aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
2401 cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt
2461 ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata
2521 tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc
2581 tggccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
2641 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
2701 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
2761 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
2821 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
2881 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
2941 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
3001 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa
3061 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg
3121 gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
3181 cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
3241 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
3301 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
3361 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
3421 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
3481 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
3541 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
3601 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
3661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
3721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
3781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
3841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
3901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
3961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
4021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
4081 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
4141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
4201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
4261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
4321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
4381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
4441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
4501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
4561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
4621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
4681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
4741 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
4801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
4861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
4921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
4981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
5041 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
5101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
5161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
5221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
5281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
5341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
5401 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
5461 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc
5521 atcgataagc tttaatgcgg tagtttatca cagttaaatt gctaacgcag tcaggcaccg
5581 tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta
5641 ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac
5701 agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc
5761 gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg
5821 ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatccat
5881 ttttttaatt aaataaagga attaatattg atttgataga gcatgtaatc aataacttct
5941 atgaaaatgt tgaaaaacaa tacacggaaa cacttaactt acaattaaat tgttaaagga
6001 gatgatgata agaatggtca gtcaccagat aaaatgatct ctggtcaatt ttaggaaagc
6061 tgaaaaaacc tgaccaaatg cgtcgtaaat tctatgctta gaaagactcg gtgccagtgt
6121 aatacctaag atatggtttt tatttccata tctgttcgaa aacttagaaa taacaagttc
6181 aacaataacc agggacttta aatatgccta gatatgctaa ttatcagata aaccccaaac
6241 agaatattaa aaattcacat gggaaatctt catcgtcaga tttttctagt gggtaccttt
6301 cattctcaaa taattcgctt gatgaccctt ttattcggca gcaagttaag agagaattta
6361 tttgggaggg acatatgaag gagattgaag aagcttcaag attaggtaat tttgctgtca
6421 gttttcgggc tgcgggcggg ccaacactca gagctttggg caaaggtgcg gcagccaagg
6481 ggcatgatat tttggaaaaa accattaaac ccggctcaat taacaaagct tatcccaaag
6541 acgaagcttc agatgtaata aagaaagtac aggaagcggg tattgaaggc tatgtaggcc
6601 attgggataa aaaaactggc cgccttttgg gtatttacat gagtagtggt cacggtttat
6661 cagatgaaca ggtcaatgga aaaatttatc ctatagactt aaacaatctg gaagcgagtc
6721 tctcagcctt gaaagcaaaa gagaactggg ctgcactacc ttttactggt gactatgata
6781 tgcatgatat gattagtttc acaggtcaac ctcattcagt accatccaac tcatcagagg
6841 aaaggaaaat tatagatcgc attaaccgat tggttgctcg atctgattca aaccggccat
6901 ttggagatat agagcataat gttatcaggc atggagccca ggtaagctac ccggcttttg
6961 ctatggataa agaaaaagag gaaataaaaa aacacggggg gattgtcaag gctgttgctg
7021 aacccggtga atttcctgtc gctattgtta gcaaaggaaa atggacaatt gccaacaaca
7081 ttgatgaact aaatcagttt tataatagta tcggtgcaaa aatgaaagtg agctggaagc
7141 cgggtgctga aaatcctgga ttcgtttcta atccacaaag accgggcatg gccagattta
7201 gccggaaaag atgatatgga ttttcatgtc atcaggagaa tattcaaaat tctgtgtcac
7261 acaaaatatt ttacagcgta atcacaccat taagaggggc atcaaaataa atggttaact
7321 gggacaatgt tgaatttcat ttttataagg cattgtccgt attatccacc atccagatca
7381 acggattcag atggtgtaaa gaaagcatat agctggtagg taaaattaag aagtgtagtt
7441 ttataaagta ttaatggaga acgatgaatt taaagtaaat ggaggaaggt tagtttcact
7501 atcaaattac aggaccaaaa atatggagta ttccatcttc tttggtgaat taagtcttga
7561 agacttcaac aaatattgcc tggaactatg ggcctgtgaa tggtattgga gtatcattta
7621 taacgactat ttcgatagga aatttaaagt cttgttgaaa atgactaatc gtctaaaagt
7681 atgtctggag aaataggttt aatctctata tttaacggtt ttattttcat ctatttacta
7741 atggtaaatt tttttactct ttgaatatat tggtgagttt gttatcccca ttagttacca
7801 gggtattatt aatgagtgaa attcaatttt attgatatta aagtcaaatt tacaaatgat
7861 gactttatta ttttttattt attaatttta aattattttt tgttaatctt tactataaat
7921 gaattttttg cttgcatgga cttgattttt ttatatataa cattgacaaa tattaattca
7981 tatatatgat tgttatagtg agttatttaa ttgattcgac taacaatttc attgtaattt
8041 ttcaagatag cagtcaattg ctataacaag catctttctc gtcaaaggaa aatgtgtatg
8101 gcctataagc agcaacttga aagatgaagg atataatgat gattaactta acggagagta
8161 gatattagaa gtagtagaag gacaatttaa gactttatac ccattaaaac aaaggggtaa
8221 aataatgatg tttgatgaaa atttagaatg tgttaatcac gaaattatca atgaaaaatt
8281 agatacgaaa attcaccttg ctaatcctta tgccttttgg gggggaaggg taaatgagaa
8341 tataaatggg ttaattaaac aacattcttt aagaggaact gactttaata aaatttttaa
8401 ccggaagata aatttttttg taaatggaat aaataattgc ccccgaaaaa ctcggaatat
8461 aaaaccccct aattaattat ttaaaggaat aagagtttat ttgctcgtag agtaattatg
8521 ttccattaat atgctaaatt gctcagtaaa gttattaact ttttttcaac gccagtgaat
8581 aagtctgttt tattaaataa ggagttcagg tgtttatttc aaaagaatta tcaagcttta
8641 ttgctgtagc taaaaataaa tcaataaata aagcaacaac ggaacttttt attacacagt
8701 caccgatttg ccgaagctta aaaaaactgg aattttcatt aggtgtaaaa ctctttgtaa
8761 gaaaaagcca gggattggct ttaacagaag aaggagaatg cttatataat aaagttttgt
8821 caatttgtga acggcttcaa gaaatagaag gtgactacaa gaatgaaaaa tcaacataca
8881 atgatctata tcaaaaaaat atatgaggtt ctaatataat aagcggttct agataactaa
8941 agtcatctat taccaatatc tgtatactca gtgaaagttt tgtcttctag gtaagttttc
9001 ataatagtga cataccttat tttttcatct gggtgtctct catcctgaag aatgggtgat
9061 atagttgaaa taactcagtt cgttttgtgg tttacg
//
