质粒名称: | pAWP78-PVCpnf1-16 |
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出品公司: | addgene |
目录编号: | 198271 |
存储实验室: | Feng Zhang |
载体骨架基本信息 |
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载体名称: | pAWP78 |
完整质粒大小: | 25323 bp |
载体类型: | 细菌细胞表达 |
载体抗性: | Kanamycin, 50 μg/mL |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 低拷贝 |
插入基因信息 |
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插入基因名称: | PVCpnf1-16 |
物种来源: | Photorhabdus asymbiotica |
克隆方法: | Gibson Cloning |
文献引用方法 |
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材料和方法部分: | pAWP78-PVCpnf1-16 was a gift from Feng Zhang (Addgene plasmid # 198271 ; http://n2t.net/addgene:198271 ; RRID:Addgene_198271) |
参考文献部分: | Programmable protein delivery with a bacterial contractile injection system. Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. 10.1038/s41586-023-05870-7 PubMed 36991127 |
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载体基本信息
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载体简介
载体序列
LOCUS pAWP78-PVCpnf1-16 25323 bp ds-DNA circular SYN 27-MAR-2023 DEFINITION Unmodified pPVC; contains WT PVC structural/accessory genes. ACCESSION . VERSION . KEYWORDS pAWP78-PVCpnf1-16 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 25323) AUTHORS Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F TITLE Programmable protein delivery with a bacterial contractile injection system JOURNAL Nature March 29, 2023 REFERENCE 2 (bases 1 to 25323) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 27, 2023 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..25323 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(1..1149) /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" CDS complement(1421..1792) /codon_start=1 /gene="traJ" /product="oriT-recognizing protein" /label=traJ /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE KQDELGKVMMGVVRPRAEP" oriT 1825..1934 /note="incP origin of transfer" rep_origin 2087..2718 /label=oriV /note="incP origin of replication" rep_origin 3027..3615 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3516..3535 /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(24205..25020) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 24928..24947 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 25012..25031 /label=pENTR-R /note="pENTR vectors, reverse primer" ORIGIN 1 ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc 61 tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc 121 cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct 181 gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa 241 tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca 301 acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc 361 caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca 421 ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc 481 gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg 541 ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac 601 gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt 661 gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg 721 ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt 781 cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt 841 tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc 901 cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg 961 gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc 1021 gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc 1081 atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt 1141 ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta 1201 ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa 1261 gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg 1321 cacgaatacc agctccgcga agtcgctctt cttgatggag cgcatgggga cgtgcttggc 1381 aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct gccctcgggc 1441 ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca 1501 gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt 1561 tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg cggacgtgct 1621 catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg taggcctaca 1681 ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct ggaaggcagt 1741 acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc 1801 cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca 1861 ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt 1921 cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt 1981 ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga 2041 ccccgaagca gggttatgca gcggaaaaga tccgtcgacc ctttccgacg ctcaccgggc 2101 tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc 2161 cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc gcggaaaact 2221 tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg 2281 cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct 2341 cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc 2401 tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg 2461 cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat 2521 ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg 2581 gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta 2641 accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc 2701 gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg cccctcggcc 2761 gcgaacggcc tcaccccaaa aatggcagcc aagctgaccc gctagggacg tgaagtcgat 2821 tccttcgatg gttagcaatc aaagactcag agtgctgtct ggagcgtgaa tctaacggta 2881 cgtatctcga ttgctcggtc gctattcgca ctctgcgaaa gttcgtaccg ctcattcact 2941 aggttgcgaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 3001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 3061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 3121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 3181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 3241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 3301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 3361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 3421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 3481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 3541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 3601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 3661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 3721 gcctttgagt gagctgtcgc gaatgcatct agatataatt acatcttcat cattcggtat 3781 tttaaatgac atatatcctc cttccgaata gttttaatgg tgatgttcga ttggacagtg 3841 ttttccatta ttaaaatttt aagtatcaca tcatgtttaa tgaaggattg atgtttatta 3901 gaatattcat aatagctttg tctttgcctc tatgtaaatg aaattgcata aaaagcaaat 3961 tgcattattg gcaatgtact tattctggat ttttcggcac gataaattat tggtgtaagt 4021 tttatatcat gagtaaaaat tcggaatgat attcctgtca ttttgtggcg gtagcgttac 4081 tcaagatatt atcctacatc atatgttgat tattgtaatc aatcgagcta ttttttgtct 4141 aaaaaggggc ttttatgtct acaagtacat ctcaaattgc ggttgaatat cctattcctg 4201 tctatcgctt tattgtttct gtcggagatg agaaaattcc atttaatagt gtttcaggat 4261 tagatattag ttatgacacc attgaatacc gagatggtgt tggtaattgg ttcaaaatgc 4321 cgggtcagag tcagagcact aatatcacct tgcgtaaagg cgttttcccg gggaaaacag 4381 aactgtttga ttggattaac tctattcagc ttaatcaggt agagaaaaag gatattacca 4441 tcagtttaac taatgatgca ggtaccgaat tattaatgac ctggaatgtt tctaatgctt 4501 ttcccacttc attgacttca ccttcatttg atgccaccag taatgatatt gcagtacagg 4561 aaattacgct gatggcagat cgggtgatta tgcaggctgt ttgaagcatt gatatttaat 4621 catctcatat aagggaactt ttatgacaac cgttaccagt tatcctggcg tttatattga 4681 agaattaaat agcctggcct tgtcagtttc aaatagcgcc acagcggttc ctgtttttgc 4741 tgtggacgaa caaaaccaat atattagtga agataatgca atccgtatta attcgtggat 4801 ggattatctt aatctgattg gcaattttaa taatgaagac aaattagatg tttctgtgcg 4861 tgcttatttt gccaatggag gtggatattg ttatctcgtc aaaacaacga gtttagaaaa 4921 aattattcca accttggatg atgtaacctt attggttgct gcgggcgaag atattaaaac 4981 gacagtagat gttttatgtc agccaggaaa agggttattc gcagtctttg atggccctga 5041 aacagagttg actatcaacg gtgcggaaga ggcaaaacaa gcctataccg ccacaccatt 5101 cgctgcggtt tattatcctt ggttgaaagc ggattgggct aacatagata ttccacccag 5161 tgcagtgatg gcgggagttt atgcatcggt ggatttatcc cgtggtgtat ggaaagcgcc 5221 tgccaatgtt gcgttgaaag ggggcctgga acctaaattt ttagtcacgg atgaattgca 5281 gggtgaatat aacactggcc gcgctatcaa tatgattcgt aatttcagta acacaggtac 5341 tacggtttgg ggtgcaagaa ccctggaaga taaagacaat tggcgttatg ttccagtgcg 5401 acgcttgttt aattctgtgg agcgggatat caagcgtgcc atgagctttg ctatgttcga 5461 gcctaataat cagcctactt gggagcgggt acgggcggcg attagcaact acctttatag 5521 cctgtggcaa caggggggat tagctggcag caaagaagaa gacgcttatt ttgtgcaaat 5581 tggtaaaggt ataacgatga cacaggagca gattgatgca gggcaaatga ttgttaaagt 5641 cggtttggct gctgtacggc ctgcggaatt tatcattctc cagtttacgc aagatgtaga 5701 acagcgttaa tcatatgatt atgaggagtt atcatgtctg ctattctgaa agcgcctggc 5761 gtttatattg aagaagacgc ttccctagcg ttgtctgtca gtaacagcgc gactgccgtg 5821 cctgttttta tcggaaaatt tactccgaca gtggttgatt caatccaagt ctgtacccgt 5881 atcagcaact ggcttgaatt cacttcctct ttttccctag ctccaacagt tgagattgtt 5941 gtccaatcta acactgaatc tgaatctgaa tctgaaactt accactatat tgagacaatc 6001 aatttatctc cagctgtgga agcattgcga ctctattttc aaaatggcgg aggagcttgc 6061 tatatctacc cattaaatga tgctgaagat gaattggttc tggcggccat accagaagtc 6121 attgaacaga aaggtgatat tactctgttg gtttgcccgg aactcgatct ggattacaaa 6181 actaagatct atggcgcagt gagctcactg ttgaatgata acaaagtggg ctatttcctg 6241 attgcggata gcaatgatgg agaatctgtg tcaggagtat ggaatagtgc taaggccgcc 6301 gcctattatc cccagttgga aactaaccta aaattttcca cgttgcctgg ggataaggac 6361 attcgtatca gcggttatca ggatgatgat gaaacacata aaccgaaaaa cttggatgag 6421 ctcaggacaa tcaacgaggc gttggcacag gatattgatg caagattgct cgaggagaaa 6481 caacgtgctg tcatcattcc gccaagtgct gccattgcgg gcatttattg ccaaacggat 6541 aatcgtcgcg gtgtttggaa agcgccagcc aacgttgcgc tcacagggat cgggagtttg 6601 cttgataagg tagacgatga acggcaggga gagatgaatg acaagggaat caatgtcatc 6661 cgttcattta ccgaccgtgg ttttatggtc tggggagccc gtacttgtgt ggacgctgcc 6721 aacatcagct ggcgttatat tcctgttcgt cgcctgttca attccgttga acgagatatc 6781 cgccaggcgc tgcgcgctgt gttgtttgaa actaatagtc agcctacctg ggtacgtgct 6841 aaggctgccg ttgatcaata tctttatacc ctttggcaga aaaatgcatt gatgggtgct 6901 cgcccggaag aagcttattt tgtgcaaatt ggtcaggata tcaccatgtc cgaggctgat 6961 attaaacagg gtaagatgat catgactgtt ggtttggcag cagtgcggcc agctgagttc 7021 atcattctgc aatttacgca ggatgttgtt cagtaatctc catgactaaa cgccaggcac 7081 tgtattgaca gtgcctactc taaccatctt ggaggaggtg atgatgatgg agagactcca 7141 accgggtgtg actttaacag aaagtataat cacgatgggt cagcaagaga tacccagtgc 7201 tgtgccggtg tttattggtt acaccgttcg ttatccggaa caatcggaag catcagtccg 7261 tatcgacagt ttggccgagt ataccagcct gtttggtgac gaccatgtga tgatgtttgc 7321 tgtcaggcac tattttgata atggcgggca acaggcattt gttttacccc tgaaggacaa 7381 tatgccatca gtggagatga ccacagctga agcggaaaat ctgatagccg cattgcgctc 7441 tgctacggtt agcgaagcca ttggtgggca tagtcagatt acactgattt tggtaccgga 7501 tatggctcgg cttaatgaca gtgatattga tgactcctca acccaggtaa gcctgtggtc 7561 ccaaggctgg gaggcgctgc tgcaattgag tcaggttagg cccaacctct ttgtgctgtt 7621 agatgcgccg gataatgttg aacaggcgca gaagtgtatg acaacgctat cgtcagatta 7681 tcgtcaatgg ggggcagcat attggcctcg tctggaaact acctatcaga aagaaatatc 7741 tggcaaggac aatgaatctc agggaatttt ccaggggact gttctgtcac ccacagccgc 7801 ggtcgcagcg gtaattcaac gcacggataa cgacgcgggt gtttggaaag caccggccaa 7861 tattgcctta tcccaggtta ttcgacctgt taaatcttat cttcagggaa gtgtactgtt 7921 taacagcagc ggcacttcgc tcaatgtgat ccgcagtttc ccaggtaagg gcatacgggt 7981 atggggatgc cgcactctgg aaaacacgga taatacgcag tggcgctatc tgcaaacacg 8041 tcggctggtt tcctatgtaa cagcgcattt gacccaattg gctcgcatgt atgtcttcga 8101 gccaaataat gaacttacct ggatgaagtt aaaaggacaa agttacaact ggttacggca 8161 attatggttg cagggtggct tgtatggttc acaggaggat gaggcattta acattctgtt 8221 aggcgtaaac gagacgatga ctgaggatga tgttcgtgca ggaaaaatga tcatgaaagt 8281 tgagttggct gtgttgtttc ctgccgaatt tattgagatc agtttggtgt ttaataccca 8341 aacagaggcg ctgtcttaag aaggaaaaag tacgatgaac gattattaca cacccgtggt 8401 atcccatcgt tttatggcga gttttatttt taaccgcatt cccgatccgc tggatattcg 8461 ttttcagcgt atctctggcc ttagtcggga actacaggtg actcagtaca gtgagggagg 8521 agaaaatgcc cgtaataact atttagctga gaaaatccaa cacggtacgt tgactttgga 8581 acggggcgtg atgacagtct cgccattgac ctggatgttt gatcgggtat tgagtggtga 8641 aaaaatcgct tatgccgatg tggtggtgat gctactgaat gaaaattcac tgccattgtc 8701 cagttggacg ttgagcaatg cgctgccggt acgctggcaa accagcgact ttgacgctaa 8761 cagcaatgcc atattggtga atacccttga attgcgttac caggatatgc gctggcttgg 8821 agtcaaaata tgacagtaga aatcagagag ttacttatcc aggcaaaggt agtgccatca 8881 acacgaccga ctgaatcaga acggcaaaac cattctttga tacaggaaag tctggatgag 8941 gcgacttggg tggaaacgat aaaacgcgaa gtgttggccg cattacgcga tgaggaaggg 9001 tggcgtccat gagtctgatt gaacgtggtt tagctaagct gacaattaat gcttataagg 9061 atagggaagg gaagatacgg gcaggaacgt tgcaggccat gtataaccct gactccttgc 9121 aactggatta ccaaacggat tatcagcaat cccaagcgat taatagcgaa aagcaaagta 9181 gcatttatgt acaggccaag cccgcagggt tatcacttga attaattttt gatgccacga 9241 tgccgggtaa caaaaccccc attgaagagc agctcatgca gctcaagcaa ctgtgcagtg 9301 tggatgcaac cagtaacgag acgcgattcc tgcaagttaa atggggcaaa atgcgttggg 9361 aaagtcgggg ttactttgct ggcagggcca agagtttgtc tgtgaattac actttgtttg 9421 atcgtgatgc gactcccttg agggtacggg taatattggc attagtggct gatgaaagtc 9481 tggtgttgca ggagactgaa caaaatctgc aatctccggc aaaaatcgca ttacgcatac 9541 aggatggggt atctctggct ctgatggcag ccagtacggc atcaacattg tcaggcggtg 9601 tggattatct gacgctggcc tggcaaaacg gtctggataa tctcaatggg ttcgttccgg 9661 gtgaaatatt gcaggccacc aggggagacg aatcatgagc caccaactga aaattattgc 9721 agatggtaag gcactgtcac ttttggccgc ggtagatgtg gacacctgtt atcgggttaa 9781 cagtatacct tctgcgacat tgaaactgag cgtaccggat aggccactct cttctttcag 9841 tcagacggat gttcagacag aactggccca ctgtcaggta gggaaaaccc tgcgtctgga 9901 attgattgat ggtagcaaaa aatgggtgct gtttaatggt cttattaccc gtaaggctct 9961 gagaattaag aataagcaat tattgctcac tctggttgtc aagcatcggt tgcaactgat 10021 ggtggatacc cagcattcac agctgtttaa agacaaaagc gaaaaagcga tcttaagcac 10081 gctattgaat cagaccggaa tcaatgctcg cttcggaaag atagcggcgt tagatcaaaa 10141 gcatgaacag atggtgcaat ttcgttgttc agactggcat tttctgttgt gccgactgtc 10201 ggcaaccggt gcatggttgt tacctgccat agaagacgtt cagtttgttc aacctgatgc 10261 tctgaaatca aactcagcct ataccttgaa gagcaggggg gatgagaaca aagacatcgt 10321 tgtcaaggat gcttactggc agtttgacaa tcaaatcaac cccgctttgc tggaagtcag 10381 tggctgggat atcagtaagc agcaggtaca atcaggcggt cgctacggaa aaatcgcgtt 10441 gggtaaggcg gcactctctc ctgatggatt ggcatccctt aataaaacgg gttgggacat 10501 ttgttatagc agtccgttaa caacccagga aagcggttat ctggcacagg gattattgct 10561 taaccagcgc atttctgggg tgacaggaga atttttgctc aaaggagatg ggcgttacca 10621 gttgggagac aacattcagc tgactggatt tggttcacag ttagatggta cggcaagcat 10681 tactgaggtt cgccaccgtc ttaatcggcg aattgattgg gaaaccacgg tgagcattgg 10741 tttacaacat gaatatttgc cgatattacc tgatgctccc gaactacata ttgcgacagt 10801 agcgaaatat cagcaggaca gtgcggtgtt aaaccgtatc cccattattc tgccggtact 10861 gaatcgtccc aatgaatttt tgtgggccag attggggaaa ccttatgcta gccatgaaag 10921 cggtttctgt ttttacccag agccaggtga cgaagttatt attggttttt ttgaaaatga 10981 tccgcgttat ccagttattt taggtgctat gcataatccg aaaaataagg ccccttttga 11041 accaacccaa gataataggg aaaaagtatt gatcgttaaa aaaggtgaag cgcaacaaca 11101 attagtcatt gatggcaaag agaaaatgat ccgaattaat gcgggtgaaa atcaaataat 11161 gcttcagcaa gataaagaca tttctctgtc aacgaaaaaa gaattaacac tgaaagcgca 11221 gacaatgaat gccacgatgg ataaatcatt ggcaatgtcc gggaaaaaca gtgttgaaat 11281 caaaggcgca aaaattaatc ttacccaatg aaaggtgacg atgaatggaa aatcaaatac 11341 tgacacaact ctatggtcgt ggttgggctt ttcctccggt cttttccctt gaaaaggggg 11401 tagagatggc tgaaggggcg gaagatgtga gacaaagttt gcagattctg tttagtactg 11461 agccggggga acgtcttatg cgtgaaaatt atggctgcgg attaaatgat tttatgtttg 11521 aaaatatccg caatgaactt attgctgaaa ttgaatccca tatccatgac aacgtattac 11581 gatatgaacc ccgggctgat atgactgata ttcaggttcg tcaatcccct ggcatgggga 11641 atactttgca agtgcaggtc atgtatcgcc tgagagggag tgatatcaat caacaaatcc 11701 agggagtact tgcactgagt gaaggccggg tgacggaggt agtatgagtg aagcgattgt 11761 ggtggatggt gacgtgttac agtttgatcc caactttggc aatcggcagg tgacggttcc 11821 cagcccagga aaaattagcg gcacaggaca tgcgcaggta agtggaaaaa aagtgtgtat 11881 tctgggggat gagaaacagg tcagggtttc tgcaacctat attacaacaa cacatactac 11941 gccgggaaca ggaaccatta ctatcagtgc tctggatgct ggccagcagg cccttcagtg 12001 taccagtggg gcggctttaa ttatcaaggg gcagcaattt acggcgatgt ttacgcctga 12061 attgccagcc atgaataata cagtgactcc gccacaaccg gatgttacga caccttcatc 12121 aggaaaagga cgttttatca ctcaacaaaa ttttgctacc gtaaattaga gtattgactg 12181 aattaaatag aattaacgaa ggtgtaaata attatttatt tgctgacgaa tcgctgtgac 12241 aaataaacac aggtgatgtt atggaattaa atgagttaac taacaaattg tcaaatttgg 12301 tgccaatgac cgattttaaa ttagataatc gagccagttt gcaattgctt aaatatattg 12361 aagcgtatac gaagataata ccctttaatt ctggcgataa atattggaat gactttttct 12421 ttatgtcagg aaatacgcca gagaaacttg caaaattata tcagaaagaa atagaaccca 12481 atggggagtt attacctcag caggcttttt tgttggcggt tttgcgttta ttggaaacac 12541 caatatcctt attaaatgta ttacctgctg ctcatcgtga gctctattat cgggagcttt 12601 taggcttgtc ttcccatgcg gcacagcctg atcaggttgc tttatctatg gaactgaatt 12661 cgacagtgat ggaacagctg ctccctgaag gaaccctgtt tgaggctggt caggatgaac 12721 aaggcaatgc attgcaatat gccctggatg ccagtttgct ggctaatcgt ggatatatca 12781 gtgacttgcg ctggttacgg aatgacgggg aaaagcaatg ggttacttct gctccatggg 12841 atttacaggc acaggtgtca ctgccgtctg atgggatacg attatttggt aagacaaata 12901 gtgatcagca ggtatttggt ggggtgttga taacgtcatc acttctggcg atggaagcgg 12961 ggataaggaa gatcattgtt acttttgagc aggagatgaa cacccaagaa ctggtggcac 13021 aggtcagcag tggaaatcaa tggctaacat tgacgtctga ggtaaataag aaagaggtca 13081 cactgacact gtcagacaaa gaaccggcaa tcagtgcgcc agaggatctg gataatctct 13141 ttttcacgca accggtactc aggctacagg gaaaggatag tcaggcactg ccggaggtga 13201 cgggtatcag cgtttcggaa aaggatgata ctaaggatac ctcttttgag atgtatcact 13261 taacaccatt tggttatagc agtgatatag agccattgga ggaaaatcca gcgttatatt 13321 taggctttac tgatgtaaag ccagggcaaa cactggcgct gtattggaaa ttaaaatccc 13381 cgcagcaacc aaccgtttcc tggtattacc tggatcaaca taatcaatgg gctgaattgg 13441 attcatgggt cagtgatgga acccagaatc tgtatcagga tggtacttgg cacgttgagt 13501 tgcctgtgga tgcatccaat caggcagagc agatgccagt tggacgctat tggttgcggg 13561 cagtggtgga ggtacccgct catgaggggg cgttggggaa ggctccttgg ctatatggtc 13621 taatctataa cgccatgacg gcaaccttgg ttaatgtaga tagcatcagt gacagccatt 13681 tcttaacccc tttgcctgcc agcagcatac agcggcccgt tgaacccatc attgtgttgg 13741 catcggtcaa ccagccttgg gcatcatggg gtggacgtat acctgaatcc tacagtgcct 13801 tttttgaacg gatagctcaa aacctgtctc atcgaaaccg gtccttaacc tggggaaata 13861 tggtgacatt actcaaagag cgttatgtca gcatctttga tgttaagtat ccaggtaatg 13921 atgaactcac cagagtgcca gcattggagc agcagcaact aacagtgatt ccagcaaacc 13981 ggtacaacga tagcgatgat tctctgcgtc cggtactgaa tcctgctcgt ctgcaagaga 14041 tggctgattg gttgcagcag aaagactctc cctgggcctc tattgaggtc aggaatccag 14101 aatacttgga tgtgaaaatc cattacgagg tgatttttaa acctgatgtg aacgaagatt 14161 ttggctatcg ccagctacag cagcaactgt gtgaggtgta tatgccttgg agcatagatg 14221 agcagcggcc cgttgtattg aataacagca ttaattattt ccagttgtta gccactattc 14281 aacagcaacc gctggttgag cgagtcactc gtctgacact acatcgggct gattcttctg 14341 atgagagtga tggtacagca tctgtggaag ccaaagataa tgaagtgctt attttagtct 14401 gggaagagga cgataatctg caataccgag gaaatgacta tgagtaatca ggatgcactg 14461 tttcatagcg ttaaagacga tattcacttt gataccttgc tggaacaagc tcatcaggtg 14521 attgaaaaac aggctgaaaa actgtggagt gatacggcag agcatgatcc gggtatcaca 14581 tttttgcagg gaatcagtta cggtgtgtca gatttggctt accgacatac attacccctg 14641 aaagatttac tgactccggc gccggatgag cagcagcaag agggaatttt tcctgccgaa 14701 tttggcccgc ataatacact gacttgtggg ccggtgacag cggatgatta tcgcaaggca 14761 ttgttagatc tacacagcag cgacagcctg gatggtactc agcaggatga gggggatttt 14821 ctgttccgga gtgtgcaact ggtgcgtgaa ccggaaaaac agcgttatac ctattggtat 14881 gatgcaacca agagggaata tagctttgtc aacagtgaag gggctaaaga gtttaccttg 14941 cgggggaatt actggttgta tctggaacca acccgttgga ctcagggtaa tattgccgct 15001 gctaccagac aactgacaga atttttgact aaaaatcgca atattggtga atctgtcagc 15061 aacattatct ggctacaacc ggttgatctg ccactgttgc tggatgttga actggatgat 15121 gatgtaggtg cacaggatgt ccccggtatt tttgcggcgg tgtatagcac cgcagagcag 15181 tatctgatgc ctggagcaca gcgttaccgt acggaagtac tgcaaaatgc tgggatgagc 15241 aatgatcaaa tcttcgaagg tccattattg gaacatggct ggataccaga gctgccggca 15301 gcccgtgatt atactcaaag gctcactctc aatcttagcc ggttggtaaa tagtctgctt 15361 gagattgagg gcattaaaca tgtgaatcgt cttcgtctgg atgatagctt cgataaaact 15421 gctattgaac ccgttaaggg ggatacctgg tcgtggtcga tcaaagaggg ctattatcca 15481 cgtctttggg gagaagaccc acttaaccaa ttggcgcaac aaaatggccc gcttagggtg 15541 atagccaaag gagggattag cgtcagtgtg agtaaagagc aaatccaggc cagtttaccc 15601 agtcaatcac tgattcaaaa tgagccggta atattggctt acggccagca ccgtgacgtt 15661 ggcagctatt atcccgtcag tgatactttg ccgccttgct atggactaca acattctttg 15721 tctgaaagtg aacacttatt gccacttcat caatttatgt tgccatttga acaattattg 15781 gcctgtggtt gtcaacagat agccatgctc ccgcggttac tggcttttca gcgcgaaggt 15841 tatgaggttt ggggtgatca gtggcccttt aagtcaggct cagtgaatga tgacgcccat 15901 caagattatg cccctgcatt aaaggatttg ttaggacaga ttgcgctgga tagtgatcat 15961 gaattggata ttattaatta cttgctgggt tactttggca cacagcgggc accgcgtacc 16021 tttacgacac aactcgatga ttttcgtgcg gtccaacagg gttatctggc ccagcaaccg 16081 acattgactt accaccgctc caatattcgt atcgatcagg tatcgtcgct acaaaaacgt 16141 attgctgctc gcatggggct gggcggtgag ttgtttaaac ctcaaccgga tctgagccaa 16201 ctgccttttt atttgattga acatcgagcg ttgctgccag tcaaacccaa tagtcagttt 16261 gataaggaac agaaaccagc ctcggtgaca gaggaggggg gcagccaaac aggtcaacat 16321 tatgtggtca ttgaacagaa gggcattgat ggcaagctga cacaggggca agtgatcaat 16381 ttaattctgt atgaaggaga gcagggagaa acccaattta cgatacgcgg tcagatggta 16441 ttcaaaaccg agggggataa gttttggttg gatgtgaata atagtgcgca actggaatat 16501 aatctggcgc gggtaatgac agcagccaag gcgagtaaac tcttttggca aaacagcccg 16561 gtatggatgg aggatatggg ctatcgtctg gcctatgcta gtgaccaatc ctcattgcct 16621 gtgaatcaac ggcgcttgac ccgcacagtg caaactccat tcccgccgat ggttgttgta 16681 ggtagcgaaa tcaccctgtt aaagcaggtg gggatagtca atttaaaaaa agcggagtca 16741 gaaaaacttt atgcaaaagt tgttagcttt gatcgcattg aagggacctt gattattgag 16801 cgtttgggta attccactct ggcttttcct acctcggaag aggcgtggcg gtatagttgg 16861 tatttttcgg gggagaaata tgaaaggact gaccgctttt catttgtgat tagcgtagta 16921 gtgaacagtg acttaattaa attgcccggt gttgatccct ataaattgga agaatgggtg 16981 aaagaaacga ttcttaccga atttccagct catatttcta tgattatcca ttggatggat 17041 cgggaagcct ttttaaattt cgccaatacc tatcagcgtt ggcaaaataa tggtacgcca 17101 ctgggggatg cggcttattc cattctagaa agtttgacac ttggtaaatt gccatctgcc 17161 ttaaaaggtg ttggcacaat gcgtattgcc acatctagtc aaagagaaga agtggtgggt 17221 agtaatggtg atcaatggaa tacagatgga ataacccaga atgaattatt ctatgttcct 17281 aaagagagct aggaaaaata aatatctgcc actaatgatg ttgaattaaa tatgttttct 17341 ggagttaatc atgaacgaaa ctcgttataa tgcaactgta caagaacaac aaacattatc 17401 taatccaaaa gctgttggac ctgacatcga taaattaaag gataaattta aagagggcag 17461 tattcccctg caaaccgatt tcaatgagtt aattgatatt gccgatattg gacgtaaagc 17521 ctgtggtcaa gcgccacaac aaaatggccc aggagaagga ttgaaattgg ctgatgacgg 17581 tacgcttaat ttaaaaatag gcactttttc caataaagac ttttctccat taatattaaa 17641 agatgatgtt ttatctgtag atcttggtag tggtctgact aatgaaacca atggaatctg 17701 tgtcggtcag ggcgatggta ttacagttaa cactagcaat gtagctgtaa aacaaggtaa 17761 cggaattagc gttactagta gtggtggtgt tgccgttaaa gttagtgcta ataagggact 17821 tagcgttgat agtagtggtg ttgcagttaa agttaatact gataagggaa ttagcgttga 17881 tggtaatggt gttgcagtta aagttaatac tagtaaagga attagcgttg ataatacagg 17941 tgttgcagtt atagctaatg ctagtaaggg aattagcgtt gatggtagtg gtgttgcagt 18001 tatagctaat actagtaaag gaattagcgt tgatggtagt ggtgttgcag ttatagctaa 18061 tactagtaaa ggaattagcg ttgataatac aggtgttgca gttatagcta atgctagtaa 18121 gggaattagc gttgatggta gtggtgttgc agttatagct aatactagta aaggaattag 18181 cgttgatggt agtggtgttg cagttatagc taatactagt aaaggaatta gcgttgatag 18241 tagtggtgtt gcagttaaag ttaaagctaa tggcggaatt aaagtagatg ctaatggtgt 18301 tgcaattgat cctaataatg tactccccaa gggagtgatt gtaatgttct ctggcagtac 18361 tgcaccaact ggttgggcgt tatgtgatgg caataatggt acaccaaatt taatcgatcg 18421 atttatttta ggtgggaaag ggactgatat taatggagtg agtactaata cagcttcagg 18481 tactaaaaat agtaagttat tcgatttcag ttctgatgaa gctacattaa ctattgatgg 18541 taaaacactg gggagagcat tatcgttaca gcaaatacct aatcatgcac actttagtgg 18601 aataattatg gatacagaga aagttaatta ttatggaagt aaaaaaatca caacaaatgt 18661 gtggggtgta acaacaggag ataatacttc agtacgatat atttataagt catcaggtgt 18721 acttgactct aacaataatg tctccaacag taccttaggc ggaaacagtc tgcagacgca 18781 cgatcatgat attaagataa cgggcacagg aaaacattct cacaaaaaca aagtaacagt 18841 cccttattat attctggctt tcatcataaa gctttaatat atatgaaaaa ttgaaaatat 18901 aaattatcca ttaataataa agaggatatt agcatgactt cggagccaaa tctgttaaac 18961 cggattacaa ttactattga agctaataat caacaagtag ctagaaaagt attgcatggc 19021 tccttgctta atcaagctaa tataaataaa ttatttaatt catactttaa tgaatatgaa 19081 attaataggg gtgtttattt agaaacatta atcctgaatc ttggtacgat aaatttccat 19141 gattttaatt cattgtttcc tactctccta aaagctgcat tgaataaaga attcagtcaa 19201 tatcagataa acaaccatag ggaagaaatg ctatttaatg agacaatatc aaatcaagct 19261 actgataagt cttacatatt tggcgataac aaattaattg atgcagagaa tttcattcac 19321 tttttatatc aaaagcattc cacattaaat ctagtagaag caatgggaaa taatggtatt 19381 gaaaaattaa caaatcagtt aacacaaata gaaaataaat ttgcgttatt attggcaaaa 19441 agttgtttgt ctgaggaagg cttaaaacga ctcttggcta tcaaacaacc cgatttatta 19501 atcgctatca atcgcagatt atctgaaaga ataaatagac cacaatatca ggagaagctt 19561 gtttcctgcg gacaactgat atttagtgct ctgggatata tacaacagta caatatacag 19621 gaaattccta aaccggatga aaaagttatt gcacgcataa caactgaact taataataat 19681 ggtttgctta atacaatacc tattattaca ctatttcgtc agagtgggat taacgattca 19741 tcactaaatg attggctaaa gaaaatctgg caggtgagat caatttcaca gttatgcaga 19801 aagtatcttt ctgctaagga ataccaatat ctgtcagaac attttgtttc aaagagcgtc 19861 gataaaaata gatatgatga agagcccgta aatcagagca tattatcaag gttgaataat 19921 aattccatta aagaaggaaa taatcacagt caactctgta ctctcagtag actatattct 19981 gaacccgttg tattacctga acaaaccatt ctacgtcagg ttagtaatac agtagatcag 20041 agcatattat caaggttgaa taatgcctcc attaaagaag gaaataacca aagtcaactt 20101 cgcactctca gtagactata ttctgagccc gttgcattac ctgaacaaac cattccacgt 20161 caggttagta atacaggtat attaattcta tggccaatgc tacctacact atttaaccag 20221 cttggtctac ttgagaaaaa gaaatttatc catcgtcagg cccagtttaa tgccgttgat 20281 tttcttgatt acctgatttg gggaaccgaa gatgtgaaag tggaacgaaa ggttttgaat 20341 aatgttctat gtgggttaat ggctgatgaa attactgaac caatgcctat tgaaccagaa 20401 aaacaatgga taataattca atggctggac gctattatct cccaactttc tggctggaaa 20461 aagttaagtc gtaatgacgt ccgtcaatta tttctacaac gaccaggaga attactgatc 20521 aatgaacagg aaattaaaat cacaatacag caacaaccat ttgatgctct gttaactgat 20581 tggccgtggc caatgaatat ggcttgtttt agctggttga gtcaaccatt aaccattacg 20641 tggttataac cattgaccac aatgacttag tctgagtaaa aaatatgaat atatcgcctg 20701 ttttttatga ttcattgaat caggataacg accgtgatct atcgttttta tttagcgaac 20761 tggaacgaat agatctcgct cttcaacacc atttttattg tgtagaaagt cagcgaagtg 20821 agctcctgga tgagtttctg ctcactgagg cggaagtggt gaccaggctg gataagccac 20881 ttggtaaacc tcattggata aatgatgatt atctggcgat atcgcaaaag ggcaatgtaa 20941 gcctaatggc agcgtccaga ttaatggatc tgatcgaacg ctttgaactg actgattttg 21001 agcgcgatgt tttactatta ggcttattgc cccattttga tagccgctat tatcgactgt 21061 tttcgctgat tcaaggggga caacagggtc gattaccttc ttttgcgctg gcattggaac 21121 tgttttgcca ctcggcgctg gagaaacagg tacagcaagc gagttttctg caccgggcac 21181 ctttgatggg ttgccagcta ttatccatcg atactagtca aaaaacgctg gcctggctcc 21241 agactccctt tattactgac agcggggtat atcacttttt actggggcat cactacatta 21301 tgccggcttt agaacattgt gctgagtggt taacaccgac agggattggc tgttatcctg 21361 aaggattaaa acaagtactg ggtaacgtat tgttatctga caacgataat attagaccga 21421 ttgtcttatt acggggaatg gccggcagtg ccagagctta taccattact aatatgatgg 21481 cttcagaagg gaagcaaaca ctgctggtag atatatccaa acttgctgat agcgatgaaa 21541 aaaacattat tcttcagata aagcatattt tgcgggaaac ccgcatgcat ggagcatgtt 21601 tattattacg gaatttttgc ttgttagtgg aacagaataa acaactattg gactccctgt 21661 cagagttatt gaatcaacct gaattaagaa ttgtttgcct gattgagcct tattccccat 21721 tggtatggct gaaaaagata ccggtattac tgattgagat gccactttta acgcctgcgg 21781 aaaaagccag attgttaatt gccagcttac cggataattg ttccgaggat attgatacga 21841 taactttaag ccagcgttac acttttaacc cagaaaccct gccattgatt ttgcaagagg 21901 cccagcttta tcaacagcag cgagatccgc tggatatctt gcagcaatgc gatatacgcc 21961 aggcattaaa tttgcgtgct caacaaaatt tcggtcaatt ggcacagcgg attattccta 22021 agcgctcatt aaaggattta ttggtatccg atgagattgc tcagcagtta cgggaaatac 22081 tcatagcaat taagtatcgg gaacaggttc tggcgggagg gtttaaagat aaaattgcct 22141 atggcactgg tatcagcgcc ctgttttatg gtgattcagg cactggaaaa accatggcag 22201 cagaagtgat tgctgaccac attggcgttg acttaataaa agtggattta tctacagtag 22261 tgaataaata catcggtgaa acagaaaaaa acttatcccg tattttcgat ttggcggaac 22321 aggatgcagg ggtattattc tttgatgaag ctgacgcact gtttggtaaa cgcagtgaaa 22381 ctaaagattc ccaggacaga catgccaata ttgaagtttc ttacttatta cagcgcctgg 22441 agaattaccc gggtctggtc attttatcca ccaataatcg tggtcattta gacagtgctt 22501 ttaatcgtcg ttttactttc attacccgtt ttacttaccc ggatgaaaaa atccgtaaaa 22561 aaatgtggca ggaaatttgg cctagaaata taaaaatatc ggaagatatc gattttaacg 22621 aattagctca acgaacaagc gtgactggcg cgaatatccg caatattgct ttattgtctt 22681 cattctttgc ttcagagcag gggaatgatg aagtcagtaa tgaaaatatt gaaattgcat 22741 tgaagcgtga attagctaaa gtcggacgat taacatttta aaagttatca caatgaaagt 22801 attgaaatat taaataaatt tattaccaaa aagttatcac gatataattt aagagaggtt 22861 ttttatgtta aacacgcaaa ctattattga tgtcaataag gcaatggatg ccatgctgcg 22921 cgcatatctg aatcaagata ttgccattcg ttttgatcta cctgaattgg atactatgca 22981 atctgatgcg atggtaagta tctttcttta tgacattcat gaagatttac agcttcgctc 23041 ggcagaatca agagggtttg atgtttatgc cgggaggtta ttgcctggtt gggtaaatat 23101 taaatgtaac tatctgatta cctattggga agcttctaag ccagcgactg atgccagcag 23161 tccggatagc caacctgata accaggcaat acaagtgatg tcacaagtat taaatgcctt 23221 gattaataat cgtcaattgg caggtattcc tggtgcttat actcaggttg taccgcctaa 23281 agagagttta aatagcctgg ggaatttctg gcaatcactg ggtaatcgcc cacggctttc 23341 tctcaattat tcagtgacag tacctgttag cctaaacgat ggtcaggata gcgcgactcc 23401 ggttaccgcg gtttcttcta cagtggaaca aacggcatcg ctcagtcaag aagtggttag 23461 tcatgcttta cgcgaattac tcattacgga attaggagga ggagaggata accggttggt 23521 actgagtaaa gttgaattat ccgcagtgaa agagacgatg actcaagaca gtccggctca 23581 gatgattata ttgttgtctg tttcaggcat tacacgacag gaatatttga aggaaattga 23641 taatatcttt gatcgttggg taaataatgc tgaagttatt accactattg atgattgtgg 23701 gattagaatt gaaagtataa cgaaagataa tcttgtagga atttaaattt ttttaattaa 23761 ataaaggaat taatattgat ttgatagagc atgtaatcaa taacttctat gaaaatgttg 23821 aaaaacaata cacggaaaca cttaacttac aattaaattg ttaaaggaga tgatgataag 23881 aatggtcagt caccagataa aatgatctct ggtcaatttt aggaaagctg aaaaaacctg 23941 accaaatgcg tcgtaaattc tatgcttaga aagactcggt gccagtgtaa tacctaagat 24001 atggttttta tttccatatc tgttcgaaaa cttagaaata acaagttcaa caataaccag 24061 ggactttaaa tatcggatcc ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa 24121 aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc tctgccagtg 24181 ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa 24241 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg 24301 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc 24361 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag 24421 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc 24481 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac 24541 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa 24601 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac 24661 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat 24721 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag 24781 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac 24841 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata 24901 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc 24961 atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat 25021 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt 25081 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt cgacttccgg 25141 caagctatac gcgccctaga attgtcaatt ttaatcctct gtttatcggc agttcgtaga 25201 gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg 25261 ttcctgaaat gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag 25321 gcc //