出品公司: | Clontech |
---|---|
载体名称: | pLVX-DD-ZsGreen1 Reporter |
质粒类型: | 慢病毒载体;无启动子载体;荧光报告载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | 无 |
载体大小: | 8500 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | DD-ZsGreen1 (C-端) |
载体抗性: | 氨苄青霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
克隆菌株: | Stbl3 |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: |
慢病毒载体pLVX-DD-ZsGreen1 Reporter 缺少启动子元件,用于研究启动子及其它顺式转录元件在细胞内的活性; DD-ZsGreen1与ZsGreen1相比,N端融合了destabilization domain (DD)去稳定结构域,导致ZsGreen1在细胞内很快被蛋白酶降解,降低了背景荧光,是研究启动子活性的理想报告基因。 |
产品目录号: | 631752 |
稳定性: | 稳表达 |
组成型/诱导型: | -- |
病毒/非病毒: | 慢病毒 |
买家导航
pLVX-DD-ZsGreen1 Reporter载体质粒基本信息
pLVX-DD-ZsGreen1 Reporter质粒图谱载体图谱和pLVX-DD-ZsGreen1 Reporter载体序列质粒序列多克隆位点信息
pLVX-DD-ZsGreen1 Reporter质粒载体简介
pLVX-DD-ZsGreen1 Reporter质粒序列载体序列
LOCUS pLVX-DD-ZsGreen1_Reporter 8454 bp DNA circular 6-NOV-2009 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No. 631751,631752 FEATURES Location/Qualifiers LTR 1..635 /label=5'\LTR misc_feature 685..822 /label=packaging\signal misc_feature 4422..5013 /label=WPRE rep_origin complement(6322..6995) /label=pUC\origin CDS complement(7140..8136) /label=Amp(R) misc_binding 1303..1536 /label=RRE misc_binding 636..653 /label=PBS misc_feature 2028..2151 /label=cPPT/CTS LTR 5216..5852 /label=3'\LTR misc_feature 3280..3788 /label=PGK\Promoter misc_feature 3809..4408 /label=Puro(R) misc_feature 2195..2234 /label=MCS CDS 2247..2570 /label=DD CDS 2577..3269 /label=ZsGreen1 BASE COUNT 2089 a 2198 c 2253 g 1914 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgtcgagctc aagcttcgaa ttctgcagtc gacggtaccg 2221 cgggcccggg atcccgcgac tctagaatgg gagtgcaggt ggaaaccatc tccccaggag 2281 acgggcgcac cttccccaag cgcggccaga cctgtgtggt gcactacacc gggatgcttg 2341 aagatggaaa gaaagtcgat tcctcccggg acagaaacaa gccctttaag tttatgctag 2401 gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga 2461 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca 2521 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaaccggaa agatctatgg 2581 cccagtccaa gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg 2641 tggacggcca caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc 2701 aggccatcaa cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt 2761 ctgccgcctt catgtacggc aaccgcgtgt tcaccgagta tccccaggac atcgtcgact 2821 acttcaagaa ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg 2881 gcgccgtgtg catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc 2941 acgagtccaa gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga 3001 ccgacaactg ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga 3061 agggcgacgt gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg 3121 acaccgtgta caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc 3181 acaagctgac ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc 3241 acgccatcgc ctccggctcc gccttgccct agtctagata attctaccgg gtaggggagg 3301 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct 3361 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc 3421 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc 3481 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc 3541 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc 3601 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg 3661 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg 3721 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg 3781 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc 3841 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 3901 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 3961 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 4021 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 4081 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 4141 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 4201 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 4261 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 4321 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 4381 gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca 4441 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 4501 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 4561 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 4621 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 4681 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 4741 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 4801 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 4861 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 4921 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 4981 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga 5041 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct 5101 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc 5161 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga 5221 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca 5281 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac 5341 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa 5401 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag 5461 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct 5521 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg 5581 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg 5641 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct 5701 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 5761 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 5821 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 5881 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt 5941 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 6001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 6061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 6121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 6181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 6241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 6301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 6361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 6421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 6481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 6541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 6601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 6661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 6721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 6781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 6841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 7021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 7081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 7141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 7201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 7261 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 7321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 7381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 7441 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 7501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 7561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 7621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 7681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 7741 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 7801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 7861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 7921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 7981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 8041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 8101 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt 8161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 8221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 8281 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc 8341 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt 8401 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt //