出品公司: | Clontech |
---|---|
载体名称: | pLVX-DD-tdTomato Control |
质粒类型: | 慢病毒载体;荧光报告载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 9880 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | AcGFP1 (N-端) |
载体抗性: | 氨苄青霉素 |
筛选标记: | 嘌呤霉素(Puromycin) |
克隆菌株: | Stbl3 |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: |
慢病毒载体pLVX-DD-tdTomato Control组成型表达DD-tdTomato,是pLVX-DD-tdTomato Reporter载体的阳性对照载体; DD-tdTomato与tdTomato相比,N端融合了ProteoTuner destabilization domain (DD)去稳定结构域,导致tdTomato在细胞内很快被蛋白酶降解,降低了背景荧光,是研究启动子活性的理想报告基因。 |
产品目录号: | 631754 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 慢病毒 |
买家导航
pLVX-DD-tdTomato Control载体质粒基本信息
pLVX-DD-tdTomato Control质粒图谱载体图谱和pLVX-DD-tdTomato Control载体序列质粒序列多克隆位点信息
pLVX-DD-tdTomato Control质粒载体简介
pLVX-DD-tdTomato Control质粒序列载体序列
LOCUS pLVX-DD-tdTomato Control 9833 bp DNA circular 6-NOV-2009 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No. 631753,631754 FEATURES Location/Qualifiers LTR 1..635 /label=5'\LTR misc_feature 685..822 /label=packaging\signal promoter 2185..2787 /label=CMV\IE\Promoter misc_feature 5801..6392 /label=WPRE rep_origin complement(7701..8374) /label=pUC\origin CDS complement(8519..9515) /label=Amp(R) misc_binding 1303..1536 /label=RRE misc_binding 636..653 /label=PBS misc_feature 2028..2151 /label=cPPT/CTS LTR 6595..7231 /label=3'\LTR misc_feature 4659..5167 /label=PGK\Promoter misc_feature 5188..5787 /label=Puro(R) CDS 2880..3203 /label=DD CDS 3210..4637 /label=tdTomato BASE COUNT 2418 a 2609 c 2641 g 2165 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagaa 2881 tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc 2941 agacctgtgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtc gattcctccc 3001 gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg 3061 aagaaggggt tgctcagatg agtgtgggtc agagagccaa actgactata tctccagatt 3121 atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg 3181 atgtggagct tctaaaaccg gaaagatcta tggtgagcaa gggcgaggag gtcatcaaag 3241 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg 3301 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 3361 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca 3421 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg 3481 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg 3541 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc 3601 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc 3661 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg 3721 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc 3781 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca 3841 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggcaccg 3901 gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca 3961 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgaattcg 4021 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 4081 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg 4141 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc 4201 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga 4261 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca 4321 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg 4381 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga 4441 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc 4501 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact 4561 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca 4621 tggacgagct gtacaagtag gcggccgcga ctctagataa ttctaccggg taggggaggc 4681 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 4741 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 4801 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 4861 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct 4921 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 4981 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 5041 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 5101 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 5161 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc 5221 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 5281 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5341 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 5401 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 5461 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5521 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5581 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5641 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5701 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 5761 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa 5821 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 5881 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 5941 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 6001 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 6061 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 6121 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 6181 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 6241 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 6301 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 6361 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa 6421 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta 6481 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca 6541 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa 6601 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa 6661 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc 6721 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa 6781 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga 6841 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg 6901 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg 6961 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc 7021 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg 7081 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 7141 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 7201 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 7261 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt 7321 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 7381 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 7441 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 7501 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 7561 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 7621 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 7681 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 7741 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 7801 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 7861 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 7921 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 7981 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 8041 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 8101 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 8161 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 8221 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 8281 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8341 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 8401 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 8461 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 8521 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 8581 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 8641 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8701 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 8761 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 8821 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 8881 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 8941 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 9001 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9061 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9121 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9181 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9241 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9301 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9361 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 9421 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 9481 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt 9541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 9601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 9661 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct 9721 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta 9781 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt //