| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-DD-tdTomato Control |
| 质粒类型: | 慢病毒载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 9880 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | AcGFP1 (N-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
慢病毒载体pLVX-DD-tdTomato Control组成型表达DD-tdTomato，是pLVX-DD-tdTomato Reporter载体的阳性对照载体； DD-tdTomato与tdTomato相比，N端融合了ProteoTuner destabilization domain (DD)去稳定结构域，导致tdTomato在细胞内很快被蛋白酶降解，降低了背景荧光，是研究启动子活性的理想报告基因。 |
| 产品目录号: | 631754 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-DD-tdTomato Control 9833 bp DNA circular 6-NOV-2009
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 631753,631754
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
promoter 2185..2787
/label=CMV\IE\Promoter
misc_feature 5801..6392
/label=WPRE
rep_origin complement(7701..8374)
/label=pUC\origin
CDS complement(8519..9515)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
misc_feature 2028..2151
/label=cPPT/CTS
LTR 6595..7231
/label=3'\LTR
misc_feature 4659..5167
/label=PGK\Promoter
misc_feature 5188..5787
/label=Puro(R)
CDS 2880..3203
/label=DD
CDS 3210..4637
/label=tdTomato
BASE COUNT 2418 a 2609 c 2641 g 2165 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagaa
2881 tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc
2941 agacctgtgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtc gattcctccc
3001 gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg
3061 aagaaggggt tgctcagatg agtgtgggtc agagagccaa actgactata tctccagatt
3121 atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg
3181 atgtggagct tctaaaaccg gaaagatcta tggtgagcaa gggcgaggag gtcatcaaag
3241 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
3301 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
3361 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
3421 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
3481 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
3541 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
3601 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
3661 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
3721 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
3781 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
3841 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggcaccg
3901 gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca
3961 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgaattcg
4021 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
4081 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
4141 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
4201 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
4261 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
4321 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
4381 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
4441 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
4501 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
4561 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca
4621 tggacgagct gtacaagtag gcggccgcga ctctagataa ttctaccggg taggggaggc
4681 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
4741 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
4801 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
4861 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
4921 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
4981 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
5041 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
5101 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
5161 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
5221 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
5281 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
5341 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
5401 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
5461 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
5521 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
5581 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
5641 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
5701 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
5761 tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa
5821 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
5881 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
5941 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
6001 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
6061 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
6121 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
6181 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
6241 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
6301 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
6361 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
6421 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
6481 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
6541 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa
6601 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
6661 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
6721 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
6781 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
6841 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
6901 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
6961 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
7021 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
7081 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
7141 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
7201 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
7261 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt
7321 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
7381 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
7441 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
7501 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
7561 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
7621 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
7681 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
7741 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
7801 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
7861 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
7921 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
7981 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
8041 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
8101 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
8161 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
8221 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
8281 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
8341 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
8401 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
8461 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
8521 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
8581 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
8641 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
8701 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
8761 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
8821 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
8881 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
8941 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
9001 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
9061 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
9121 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
9181 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
9241 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
9301 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
9361 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
9421 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
9481 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt
9541 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
9601 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
9661 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
9721 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
9781 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
