| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-DD-tdTomato Reporter |
| 质粒类型: | 慢病毒载体；无启动子载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 9246 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | DD-tdTomato (C-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
慢病毒载体pLVX-DD-tdTomato-Reporter没有启动子元件； DD-tdTomato与tdTomato相比，N端融合了ProteoTuner destabilization domain (DD)去稳定结构域，导致tdTomato在细胞内 很快被蛋白酶降解，降低了背景荧光，是研究启动子活性的理想报告基因。 |
| 产品目录号: | 631754 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-DD-tdTomato_Reporter 9200 bp DNA circular 6-NOV-2009
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 631753,631754
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
misc_feature 5168..5759
/label=WPRE
rep_origin complement(7068..7741)
/label=pUC\origin
CDS complement(7886..8882)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
misc_feature 2028..2151
/label=cPPT/CTS
LTR 5962..6598
/label=3'\LTR
misc_feature 4026..4534
/label=PGK\Promoter
misc_feature 4555..5154
/label=Puro(R)
misc_feature 2195..2234
/label=MCS
CDS 2247..2570
/label=DD
CDS 2577..4004
/label=tdTomato
BASE COUNT 2262 a 2444 c 2487 g 2007 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgtcgagctc aagcttcgaa ttctgcagtc gacggtaccg
2221 cgggcccggg atcccgcgac tctagaatgg gagtgcaggt ggaaaccatc tccccaggag
2281 acgggcgcac cttccccaag cgcggccaga cctgtgtggt gcactacacc gggatgcttg
2341 aagatggaaa gaaagtcgat tcctcccggg acagaaacaa gccctttaag tttatgctag
2401 gcaagcagga ggtgatccga ggctgggaag aaggggttgc tcagatgagt gtgggtcaga
2461 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca
2521 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaaccggaa agatctatgg
2581 tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct
2641 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
2701 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
2761 tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg
2821 attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
2881 acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca
2941 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca
3001 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
3061 tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct
3121 acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca
3181 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc
3241 accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct
3301 cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg
3361 agggctccat gaacggccac gaattcgaga tcgagggcga gggcgagggc cgcccctacg
3421 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
3481 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
3541 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
3601 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
3661 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
3721 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
3781 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
3841 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
3901 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
3961 gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaggcg gccgcgactc
4021 tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
4081 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
4141 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
4201 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
4261 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
4321 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
4381 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
4441 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
4501 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc
4561 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
4621 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
4681 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
4741 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
4801 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
4861 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
4921 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
4981 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
5041 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
5101 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt
5161 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
5221 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
5281 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
5341 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
5401 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
5461 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
5521 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
5581 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
5641 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
5701 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
5761 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
5821 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
5881 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
5941 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc
6001 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
6061 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
6121 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
6181 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
6241 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
6301 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
6361 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
6421 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
6481 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
6541 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
6601 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
6661 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat
6721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
6781 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
6841 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
6901 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
6961 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
7021 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
7081 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
7141 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
7201 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
7261 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
7321 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
7381 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
7441 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
7501 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
7561 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
7621 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
7681 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
7741 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
7801 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
7861 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
7921 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
7981 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
8041 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
8101 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
8161 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
8221 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
8281 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
8341 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
8401 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
8461 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
8521 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
8581 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
8641 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
8701 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
8761 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
8821 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
8881 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat
8941 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
9001 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
9061 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
9121 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
9181 tgtactacaa acttagtagt
//
