| 质粒名称: | pBFC0619 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 186690 |
| 存储实验室: | Jennifer Doudna |
|
载体骨架基本信息 |
|
| 载体名称: | R6K conditional origin, bla, RP4 oriT |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 12606 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素和庆大霉素，100和10μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | EC100D pir+ |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | tnsA tnsB tnsC tnsD tniQ cas8-cas5 fusion cas7 cas6 Vc_2xBsaI_NT (Guide Stuffer) crRNA Tn7 transposon GmR+sfGFP VcDART Cargo |
| 物种来源: | 霍乱弧菌 |
|
文献引用方法 |
|
| 材料和方法部分: | pBFC0619 was a gift from Jennifer Doudna (Addgene plasmid # 186690 ; http://n2t.net/addgene:186690 ; RRID:Addgene_186690) |
| 参考文献部分: | Species- and site-specific genome editing in complex bacterial communities. Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL, Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM, Banfield JF, Doudna JA. Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7. Epub 2021 Dec 6. 10.1038/s41564-021-01014-7 PubMed 34873292 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBFC0619 12606 bp ds-DNA circular
SYN 26-SEP-2022
DEFINITION VcDART under control of Lac promoter, Vc_2xBsaI_NT
gRNA, GmR+sfGFP
cargo.
ACCESSION .
VERSION .
KEYWORDS pBFC0619
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12606)
AUTHORS Rubin BE, Diamond S, Cress BF, Crits-Christoph A,
Lou YC, Borges AL,
Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R,
Smock DCJ, Tang
K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R,
Deutschbauer AM,
Banfield JF, Doudna JA
TITLE Species- and site-specific genome editing in complex
bacterial
communities.
JOURNAL Nat Microbiol. 2022 Jan;7(1):34-47. doi:
10.1038/s41564-021-01014-7.
Epub 2021 Dec 6.
PUBMED 34873292
REFERENCE 2 (bases 1 to 12606)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 26, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12606
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 89..119
/label=lac promoter
/note="promoter for the E. coli lac operon"
RBS 120..131
/note="strong bacterial ribosome binding
site (Elowitz and
Leibler, 2000)"
CDS 2216..2227
/codon_start=1
/product="Factor Xa recognition and
cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 8716..8744
/gene="intI1 (promoter lies within the
coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
CDS 8933..9466
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/label=GmR
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
RBS 9825..9836
/note="strong bacterial ribosome binding
site (Elowitz and
Leibler, 2000)"
CDS 9843..10556
/codon_start=1
/product="GFP variant that folds robustly
even when fused
to poorly folded proteins (Pedelacq et al.,
2006)"
/label=superfolder GFP
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
terminator 10568..10639
/label=rrnB T1 terminator
/note="transcription terminator T1 from the
E. coli rrnB
gene"
terminator 10655..10682
/label=T7Te terminator
/note="phage T7 early transcription
terminator"
primer_bind 10693..10712
/label=T7
/note="T7 promoter, forward primer"
promoter 10693..10711
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA
polymerase"
oriT 10855..10963
/note="incP origin of transfer"
promoter 11156..11260
/gene="bla"
/label=AmpR promoter
CDS 11261..12121
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin,
carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(11479..11498)
/label=Amp-R
/note="Ampicillin resistance gene, reverse
primer"
rep_origin 12157..12545
/label=R6K gamma ori
/note="gamma replication origin from E.
coli plasmid R6K;
requires the R6K initiator protein pi for
replication"
ORIGIN
1 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
tgagttttcg
61 ttccactgac acctgcccga accatatctt tacactttat gcttccggct
cgtatgttga
121 aagaggagaa aggatcaatg gcgacaagtt tacctacgcc ctcagcaatt
acgacttcgg
181 cgttagagta tgcattccat actcccgctc gcaatctaac gaaatctcgc
ggaaaaaaca
241 ttcatcgtta tgtcagtgta aagatgagta agaggattac ggtagaatct
actctagagt
301 gtgatgcctg ctatcacttt gattttgagc caagtattgt tcgcttttgc
gctcaaccga
361 ttcgattttt atattatctc aatggtcagt ctcactccta tgttcctgac
tttctagttc
421 aatttgatac caacgagttt gttctatatg aagtaaagtc agcttatgct
aagaacaaac
481 ctgattttga tgttgaatgg gaggcgaaag taaaagcagc aactgaacta
gggctagaat
541 tggagcttgt tgaagagagt gatattaggg atacggttgt attaaataat
cttaagcgca
601 tgcatcgtta tgcttcgaaa gatgagctga ataacgtaca taactctctc
ttaaaaataa
661 taaagtacaa tggcgcccaa tctgcaagat gcttgggaga acagttgggt
ttaaaaggcc
721 gaactgtttt accaattttg tgcgatttgc tgtcaaggtg tttactcgat
acacgtttgg
781 ataagcctct atctcttgaa tctcgatttg agttggccag ttatggctaa
gaaagggttc
841 tcaagtttcc atagaaaagc agtctcttcc caagatacgc ttgaatcgat
agagcttgtc
901 tctagcgcta attgtctaga aagtgttacg tatcaagata tatcagcatt
tcccgaaaca
961 attgcggtag agattaattt ccgattaagc attcttcgct ttttagcgcg
aaagtgcgaa
1021 accattgtgg ccaaatcaat tgaaccacat cgtgtagagc tacagcaaaa
ctatagtaga
1081 aaaataccca gtgcaataac gatatatcga tggtggcttg cttttcgaaa
atcagactac
1141 aaccccatta gcttagcacc taatatcaag gatagaggta atagagagac
aaaagtgtca
1201 acagttgttg attctattat ggaacaggca gttgaaagag ttatatctgg
acgaaaagtc
1261 aatgttagct ctgcatataa acgtgttcga cgaaaagttc gtcaatacaa
tctaactcat
1321 ggaacgaaat acacgtatcc taagtacgaa tctgtaagaa agcgagtaaa
aaagaaaacc
1381 ccatttgagt tattagccgc agggaaaggg gagagagtag ctaagagaga
gtttcgccga
1441 atgggaaaaa agatcctcac gtctagcgtg ctagagaggg ttgaaataga
tcacactgtc
1501 gttgaccttt ttgcagtaca cgaagagtat cgaatcccat tgggccgacc
ttggcttact
1561 caattggttg attgttacag taaagctgta atcggttttt atttaggttt
cgagcctcct
1621 agctatgtgt cggtttccct tgcacttaag aatgcaatac aacgcaaaga
tgacttaatc
1681 tcctcgtatg aatcgatcga gaatgaatgg ctatgttatg gcatcccaga
cctactcgta
1741 actgataatg gtaaagagtt tttgtcgaaa gcatttgatc aagcatgtga
atcactattg
1801 atcaatgtgc atcaaaataa agttgaaacg cccgacaaca aacctcatgt
tgaacgtaac
1861 tacgggacta ttaatacttc tctgttagac gatttacctg ggaaatcctt
cagccagtac
1921 cttcaaagag aagggtacga ctctgtggga gaagctaccc ttacactcaa
tgagattaga
1981 gaaatttact taatttggtt ggtggatatt tatcataaaa aacccaatca
gagaggcact
2041 aattgtccta atgttgcgtg gaaaaagggt tgtcaagaat gggaaccaga
ggagttctct
2101 ggttctaaag acgaattaga ctttaaattt gctattgttg attacaaaca
acttactaaa
2161 gtagggataa ctgtctacaa agaactgagt tatagcaatg accgtttagc
tgaatataga
2221 gggaagaaag gaaaccataa agttcagttc aagtataacc ctgagtgtat
ggcagttatt
2281 tgggtgttgg atgaggatat gaatgagtac tttacagtta atgcgattga
ctacgaatat
2341 gcaagtagag tatcactttg gcaacataaa tataacatga aatatcaagc
agaactaaat
2401 tcagcagaat atgatgagga caaggaaatt gatgcagaaa taaaaattga
agaaatcgca
2461 gatcgttcaa ttgttaagac taacaaaatc agagctcgga ggcgtggcgc
taggcatcaa
2521 gagaatagcg caagggctaa gtcaatcagt aatgcgaacc cggcctcgat
acaaaaacat
2581 gaagatgaaa tcgttagtgc agataatgac gattgggata ttgattatgt
ctgagaaatc
2641 gtcaaatgag tgaaacgcgt gaggctcgta tatcaagagc taaaagggca
tttgtatcca
2701 caccgagcgt taggaaaatc ttgagttaca tggatagatg tagagatcta
tcagacctag
2761 agtctgagcc tacatgcatg atggtctatg gtgcttcggg tgtaggtaaa
acgaccgtca
2821 tcaagaaata cttaaatcaa aacagaagag agtccgaagc cgggggcgat
ataataccgg
2881 ttttgcatat tgagttgcca gacaatgcga agccagtaga tgcagcaagg
gaattgctgg
2941 ttgaaatggg tgacccgcta gcactttatg aaactgactt agctagattg
acgaaaagac
3001 tgactgaatt aatccctgcg gtcggcgtga agctgattat tatcgatgag
ttccaacatt
3061 tggtggaaga aaggtcaaat cgggttctta cccaagtagg taattggcta
aaaatgatac
3121 ttaacaaaac gaaatgtcca attgttatat ttggtatgcc atactcaaaa
gttgtactgc
3181 aagcaaactc gcaacttcac gggcgatttt ccattcaggt tgaactgcgc
ccctttagct
3241 accagggagg tagaggtgta tttaaaactt ttttggaata ccttgataaa
gccctacctt
3301 ttgaaaaaca ggctggctta gccaacgaaa gtttgcagaa aaaattgtat
gcattctctc
3361 agggaaacat gcgttcgttg agaaacctta tttatcaagc atctatcgaa
gcaattgata
3421 atcagcatga aacgataacc gaagaagatt tcgtttttgc atcgaagttg
acatcgggcg
3481 ataaacccaa ctcatggaaa aatccttttg aggagggtgt tgaggtaaca
gaagatatgt
3541 tacgaccgcc accaaaagat attggttggg aggactattt gagacattca
accccgagag
3601 tgagtaaacc aggtagaaat aaaaactttt tcgaataacc taggctgctg
ccaccgctga
3661 gcaataacca tcttagtata ttagttaagt ataagaagga gatatacata
tgtttttgca
3721 aagacctaaa ccttacagcg atgaaagttt agaaagtttc tttatccgag
tggctaacaa
3781 aaatggctac ggtgatgtcc atcgcttcct agaagccact aaacgattcc
ttcaagacat
3841 tgaccataat ggctatcaaa cctttccgac tgatataact cggataaacc
catactcagc
3901 taaaaacagt tccagcgcac gaactgcgtc attcctgaaa cttgcacaat
tgacatttaa
3961 tgaaccgcca gagctacttg ggttggcaat taacagaaca aacatgaaat
actcgccgtc
4021 aactagcgcg gttgttagag gtgcagaagt ctttcctcgc agtttactac
ggacgcactc
4081 catcccctgc tgtcctttgt gtctgcgaga aaatggctac gcctcctacc
tttggcactt
4141 tcaggggtac gaatactgcc acagccataa cgtaccttta attaccactt
gtagctgtgg
4201 taaggagttt gactaccgag tatctgggtt aaagggcatt tgctgcaaat
gcaaggagcc
4261 tatcacctta accagcaggg agaacggtca tgaggcagcg tgtactgttt
caaactggct
4321 tgctggccat gaatctaaac ctctgccaaa tcttcctaaa agctaccgat
ggggtttagt
4381 tcattggtgg atgggtatta aagatagcga gttcgatcac ttttcgttcg
ttcaattttt
4441 ctcaaactgg ccaaggtcat tccactcgat aatcgaagat gaagtagagt
tcaaccttga
4501 gcatgctgtt gtcagcacgt ctgaattacg actaaaagat cttcttggtc
gattgttttt
4561 cggttcaatt cggttacctg agcggaatct tcaacacaat atcatccttg
gtgagcttct
4621 ctgctattta gaaaatcgtt tatggcaaga caagggatta atcgccaacc
tcaaaatgaa
4681 cgcgttagag gcgactgtaa tgttaaattg tagcctcgat cagattgcat
caatggttga
4741 acaacgcatc ttgaagccaa atcgaaaaag caagcccaac agccctcttg
atgttaccga
4801 ttatctattt catttcggcg atattttctg tctttggtta gctgagttcc
aaagcgatga
4861 gtttaaccgt tcgttttatg tgtcgaggtg gtaaatgcaa actctgaaag
aactaatcgc
4921 atccaatcct gacgacttaa caactgaact taagagagca tttcgtccac
tcacaccgca
4981 tattgcaatt gatggtaatg aacttgacgc actgacgata ttagtcaatt
taaccgataa
5041 gactgatgat cagaaagacc tgctcgatcg agccaaatgc aagcaaaaac
ttcgagatga
5101 aaaatggtgg gctagctgca taaattgcgt taattacaga caaagccata
acccaaaatt
5161 cccggatata cgttctgaag gcgtgattcg aacccaagcc ctgggtgaat
taccgagttt
5221 cctactctcg tcctcaaaaa tcccaccata ccattggtca tatagccatg
attcaaaata
5281 cgtcaacaaa agcgcattcc tcaccaatga gttttgttgg gatggtgaga
tctcatgttt
5341 gggtgagctt cttaaagatg cagatcaccc actttggaat actttgaaaa
agttaggttg
5401 ttctcaaaaa acttgcaaag caatggcaaa acaactagct gatattactc
tcacgactat
5461 caatgtcaca ctcgcaccaa attacctgac tcaaatatct ctccccgata
gtgatacatc
5521 ctacatttca ctctctcctg ttgcatcgct atcgatgcaa agccactttc
atcagaggct
5581 ccaagatgaa aatcggcata gcgcgataac gcggtttagc cgaactacca
acatgggggt
5641 cactgcgatg acatgtggcg gcgcatttag aatgttaaag tctggcgcta
agttttccag
5701 cccccctcat caccgattaa acagtaaacg aagttggttg acgtcagagc
atgttcagtc
5761 attaaaacag taccagcgcc tcaacaaaag cctcatacct gaaaactctc
ggattgcact
5821 ccgtagaaaa tacaaaatcg agcttcaaaa tatggtcaga tcttggtttg
caatgcaaga
5881 ccataccctt gattcgaaca tacttatcca acatttgaat catgacctat
cttacttagg
5941 agccacaaaa cgttttgcat acgatccagc gatgaccaag ctctttactg
agcttttgaa
6001 acgagagtta tcaaattcaa tcaataatgg tgagcaacac actaatggat
cgtttttagt
6061 cctaccgaat atcagggttt gtggcgcaac agctttaagc tccccggtaa
cggtggggat
6121 tccatcactt acagctttct ttggcttcgt tcacgcattt gaacggaata
taaatcgcac
6181 cacctcatcg tttcgtgttg aatcctttgc gatatgcgtc catcaactac
atgtcgagaa
6241 gcgaggtttg acagcagagt ttgtggaaaa aggcgacggg actatatccg
ctcccgcgac
6301 ccgggatgac tggcagtgtg atgtcgtatt tagccttatt ttgaacacca
actttgctca
6361 acatattgac caagatacgt tagttacatc actaccaaag cgattggctc
ggggttcagc
6421 aaaaattgcg attgatgact ttaaacatat caactcattc tcgacattag
aaacagcgat
6481 cgaatctctg ccaatagaag ctggtaggtg gttatcactt tacgcacagt
caaacaataa
6541 tctaagtgat ctattagcag ccatgacaga ggaccatcag ctcatggcaa
gctgcgtcgg
6601 ttaccacttg ttagaagaac ccaaagataa accaaactcc ctcagaggtt
acaaacacgc
6661 tatcgccgag tgcatcattg gactcattaa ctcaatcacc tttagctcag
agactgatcc
6721 caacacaatc ttttggtcgc taaagaacta tcaaaactac ctagtggtac
agccaaggag
6781 tatcaacgat gaaactaccg acaaatctag cctatgagcg ctctatcgac
ccatcagatg
6841 tctgtttttt tgtcgtctgg cccgatgata gaaaaacacc tttaacctac
aattctcgta
6901 ctctgctcgg gcaaatggaa gcggcatcat tagcctatga tgtctcaggt
caaccaataa
6961 aaagtgccac cgctgaggcg ttagctcaag ggaaccctca tcaagttgat
ttctgccacg
7021 ttccatacgg tgcgagtcat attgaatgca gtttctccgt ttcgttttct
tctgaactac
7081 gtcaaccata taagtgtaac tcaagcaaag ttaaacaaac gctagtgcaa
ttagtcgagc
7141 tctacgaaac gaaaatcggc tggactgagc tagcaacccg atatttgatg
aacatttgca
7201 acggtaaatg gctgtggaaa aatacccgta aagcctattg ctggaacatt
gtacttacac
7261 cttggccttg gaacggggaa aaggttggat ttgaagatat tcgtactaac
tacacctcac
7321 ggcaagactt taaaaataat aaaaattggt ctgctatagt tgaaatgatc
aaaaccgcat
7381 tttctagtac tgatgggctg gcgatatttg aagtcagggc caccttgcac
ttgccaacga
7441 atgctatggt gcggccaagc caagttttca cagaaaaaga aagtggcagt
aaaagtaaat
7501 ctaaaactca aaacagtcga gtttttcaga gtacaactat tgatggtgaa
cgatcgccaa
7561 tactaggggc ctttaaaacg ggagcagcta ttgcaaccat tgacgactgg
tatcctgaag
7621 ccactgagcc actaagggtc ggacggtttg gggttcatcg cgaagatgtc
acttgctacc
7681 gtcatccgtc taccggaaaa gattttttct cgatattaca acaagcagag
cactatattg
7741 aagtgttgag cgccaacaaa actcccgctc aagaaactat caacgacatg
cactttttaa
7801 tggctaacct gattaagggt gggatgttcc agcataaagg agactgactg
tgaaatggta
7861 ttataagaca atcacctttc tgccagagtt gtgcaacaac gagtcactgg
ctgcaaagtg
7921 tctccgcgtt ctgcatggat ttaactatca gtatgagaca cgaaatatcg
gcgtttcatt
7981 tccgctttgg tgtgatgcaa cggttggaaa aaagatttca tttgtcagca
agaacaagat
8041 agaactcgac ttactactta aacaacacta tttcgtccaa atggaacaac
ttcaatactt
8101 tcatatatcc aacactgttc tcgtcccaga agattgtaca tacgtttcct
ttagacgctg
8161 tcaatctata gataagctca cagcagcagg gctggcaagg aaaatcagac
gcctggagaa
8221 acgtgctcta tctagaggcg agcaatttga cccatcatct tttgctcaaa
aagagcatac
8281 tgcaatagcg cactaccact cacttgggga gtccagcaaa cagacgaacc
gcaactttcg
8341 actcaatatc aggatgctct cggagcaacc ccgtgagggg aactcgattt
ttagtagcta
8401 tggcttatca aattcagaaa actcgtttca gcctgtaccc ttaatctgac
ctaggctgct
8461 gccaccggtg aactgccgag taggtagctg ataacgagac ctcgtttacc
tatcggtctc
8521 gtgaactgcc gagtaggtag ctgataaccc aaaccagatg tcaacacagc
tactgttgat
8581 gcaaccataa agtgatattt aataattatt tataatcagc aacttaacca
caaaacaacc
8641 atatattgat atctcacaaa acaaccataa gttgatattt ttcagccagg
ggtccccaat
8701 aattacgatt taaatttgac ataagcctgt tcggttcgta aactgtaatg
caagtagcgt
8761 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
cggcgcagtg
8821 gcggttttca tggcttgtta tgactgtttt tttgtacagc ctatgcctcg
ggcatccaag
8881 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
cgatgttacg
8941 cagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag
gtggctcaag
9001 tatgggcatc attcgcacgt gtaggctcgg ccctgaccaa gtcaaatcca
tgcgggctgc
9061 tcttgatctt ttcggtcgtg agttcggtga cgtagccacc tactcccaac
atcagccgga
9121 ttccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg
ctgccttcga
9181 ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg
agcagccgcg
9241 tagtgaaatc tatatctatg atctcgcagt ctccggagag caccggaggc
agggcattgc
9301 caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt
atgtgatcta
9361 cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt
tgggcatacg
9421 ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaacaat
tcgttcaagc
9481 cgagatcggc ttcccggccg cggagttgtt cggtaaattg gacaacggtc
cgcgcgttgt
9541 ccttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg
gtatatccat
9601 cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
ccttggtgta
9661 tccaacggcg tcagccgaaa gtgaaacgtg atttcatgcg tcattttgaa
cattttgtaa
9721 atcttattta ataatgtgtg cggcaattca catttaattt atgaatgttt
tcttaacatc
9781 gcggcaactc aagaaacggc aggttcggat cttagctact agagaaagag
gagaaatact
9841 agatgcgtaa aggcgaggag ctgttcactg gtgtcgtccc tattctggtg
gaactggatg
9901 gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac
gcaactaatg
9961 gtaaactgac gctgaagttc atctgtacta ctggtaaact gccggttcct
tggccgactc
10021 tggtaacgac gctgacttat ggtgttcagt gctttgctcg ttatccggac
catatgaagc
10081 agcatgactt cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc
acgatttcct
10141 ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc
gataccctgg
10201 taaaccgcat tgagctgaaa ggcattgact ttaaagagga cggcaatatc
ctgggccata
10261 agctggaata caattttaac agccacaatg tttacatcac cgccgataaa
caaaaaaatg
10321 gcattaaagc gaattttaaa attcgccaca acgtggagga tggcagcgtg
cagctggctg
10381 atcactacca gcaaaacact ccaatcggtg atggtcctgt tctgctgcca
gacaatcact
10441 atctgagcac gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat
catatggttc
10501 tgctggagtt cgtaaccgca gcgggcatca cgcatggtat ggatgaactg
tacaaatgac
10561 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
ttatctgttg
10621 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
ggcctttctg
10681 cgtttatagc tgtaatacga ctcactatag gggataagag acggctcaac
gcccgtctca
10741 cagcgcaata tcaatttatg ggtgtgataa ttatcaattt atgggtgtaa
ttatcatttt
10801 atggttgtat caacaaggag gactttggca ggtgcctgca ggattcttgc
ttatcggcca
10861 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga
cagtgaagaa
10921 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac
gccgttggat
10981 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg
cgaaaaagga
11041 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag
cggaaaagaa
11101 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
atgtgcgcgg
11161 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
tgagacaata
11221 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
aacatttccg
11281 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
acccagaaac
11341 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
acatcgaact
11401 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
ttccaatgat
11461 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
ccgggcaaga
11521 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
caccagtcac
11581 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
ccataaccat
11641 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
aggagctaac
11701 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
aaccggagct
11761 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
tggcaacaac
11821 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
aattaataga
11881 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
cggctggctg
11941 gtttattgct gataaatctg gagccggtga gcgtggctct cgcggtatca
ttgcagcact
12001 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
gtcaggcaac
12061 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
agcattggta
12121 actgtcagac ccggccacga tgcgtccggc gtagaggatc tgaagatcag
cagttcaacc
12181 tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg
tttaacgtac
12241 taagctctca tgtttaacga actaaaccct catggctaac gtactaagct
ctcatggcta
12301 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat
aaaattaata
12361 taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa
aaagaatata
12421 taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg
gatgtaacgc
12481 actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac
acttaacggc
12541 tgacatgggc aagtttactc atatatactt tagattgatt taaaacttca
tttttaattt
12601 aaaagg
//
