| 质粒名称: | pBFC1207 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 186694 |
| 存储实验室: | Jennifer Doudna |
|
载体骨架基本信息 |
|
| 载体名称: | R6K conditional origin, bla, RP4 oriT |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 12605 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素和庆大霉素，100和10μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | EC100D pir+ |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | tnsA tnsB tnsC tnsD tniQ cas8-cas5 fusion cas7 cas6 Vc_2xBsaI_NT (Guide Stuffer) crRNA Tn7 transposon GmR+sfGFP VcDART CargoSbfI+AsiSI dual restriction site for donor |
|
文献引用方法 |
|
| 材料和方法部分: | pBFC1207 was a gift from Jennifer Doudna (Addgene plasmid # 186694 ; http://n2t.net/addgene:186694 ; RRID:Addgene_186694) |
| 参考文献部分: | Species- and site-specific genome editing in complex bacterial communities. Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL, Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM, Banfield JF, Doudna JA. Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7. Epub 2021 Dec 6. 10.1038/s41564-021-01014-7 PubMed 34873292 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBFC1207 12650 bp ds-DNA circular SYN 09-SEP-2022
DEFINITION VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP
cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right
End.
ACCESSION .
VERSION .
KEYWORDS pBFC1207
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12650)
AUTHORS Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL,
Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang
K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM,
Banfield JF, Doudna JA
TITLE Species- and site-specific genome editing in complex bacterial
communities.
JOURNAL Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7.
Epub 2021 Dec 6.
PUBMED 34873292
REFERENCE 2 (bases 1 to 12650)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 9, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12650
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 173..203
/label=lac promoter
/note="promoter for the E. coli lac operon"
RBS 204..215
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 2300..2311
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 8800..8828
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
CDS 9017..9550
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/label=GmR
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
RBS 9909..9920
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 9927..10640
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
terminator 10652..10723
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 10739..10766
/label=T7Te terminator
/note="phage T7 early transcription terminator"
primer_bind 10777..10796
/label=T7
/note="T7 promoter, forward primer"
promoter 10777..10795
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 10919..10939
/label=mU6-F
/note="Mouse U6 promoter, forward primer"
oriT 10983..11091
/note="incP origin of transfer"
promoter 11284..11388
/gene="bla"
/label=AmpR promoter
CDS 11389..12249
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(11607..11626)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 12285..23
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
ORIGIN
1 acaggaacac ttaacggctg acatgggcaa gtttactcat atatacttta gattgattta
61 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
121 aaaatccctt aacgtgagtt ttcgttccac tgacacctgc ccgaaccata tctttacact
181 ttatgcttcc ggctcgtatg ttgaaagagg agaaaggatc aatggcgaca agtttaccta
241 cgccctcagc aattacgact tcggcgttag agtatgcatt ccatactccc gctcgcaatc
301 taacgaaatc tcgcggaaaa aacattcatc gttatgtcag tgtaaagatg agtaagagga
361 ttacggtaga atctactcta gagtgtgatg cctgctatca ctttgatttt gagccaagta
421 ttgttcgctt ttgcgctcaa ccgattcgat ttttatatta tctcaatggt cagtctcact
481 cctatgttcc tgactttcta gttcaatttg ataccaacga gtttgttcta tatgaagtaa
541 agtcagctta tgctaagaac aaacctgatt ttgatgttga atgggaggcg aaagtaaaag
601 cagcaactga actagggcta gaattggagc ttgttgaaga gagtgatatt agggatacgg
661 ttgtattaaa taatcttaag cgcatgcatc gttatgcttc gaaagatgag ctgaataacg
721 tacataactc tctcttaaaa ataataaagt acaatggcgc ccaatctgca agatgcttgg
781 gagaacagtt gggtttaaaa ggccgaactg ttttaccaat tttgtgcgat ttgctgtcaa
841 ggtgtttact cgatacacgt ttggataagc ctctatctct tgaatctcga tttgagttgg
901 ccagttatgg ctaagaaagg gttctcaagt ttccatagaa aagcagtctc ttcccaagat
961 acgcttgaat cgatagagct tgtctctagc gctaattgtc tagaaagtgt tacgtatcaa
1021 gatatatcag catttcccga aacaattgcg gtagagatta atttccgatt aagcattctt
1081 cgctttttag cgcgaaagtg cgaaaccatt gtggccaaat caattgaacc acatcgtgta
1141 gagctacagc aaaactatag tagaaaaata cccagtgcaa taacgatata tcgatggtgg
1201 cttgcttttc gaaaatcaga ctacaacccc attagcttag cacctaatat caaggataga
1261 ggtaatagag agacaaaagt gtcaacagtt gttgattcta ttatggaaca ggcagttgaa
1321 agagttatat ctggacgaaa agtcaatgtt agctctgcat ataaacgtgt tcgacgaaaa
1381 gttcgtcaat acaatctaac tcatggaacg aaatacacgt atcctaagta cgaatctgta
1441 agaaagcgag taaaaaagaa aaccccattt gagttattag ccgcagggaa aggggagaga
1501 gtagctaaga gagagtttcg ccgaatggga aaaaagatcc tcacgtctag cgtgctagag
1561 agggttgaaa tagatcacac tgtcgttgac ctttttgcag tacacgaaga gtatcgaatc
1621 ccattgggcc gaccttggct tactcaattg gttgattgtt acagtaaagc tgtaatcggt
1681 ttttatttag gtttcgagcc tcctagctat gtgtcggttt cccttgcact taagaatgca
1741 atacaacgca aagatgactt aatctcctcg tatgaatcga tcgagaatga atggctatgt
1801 tatggcatcc cagacctact cgtaactgat aatggtaaag agtttttgtc gaaagcattt
1861 gatcaagcat gtgaatcact attgatcaat gtgcatcaaa ataaagttga aacgcccgac
1921 aacaaacctc atgttgaacg taactacggg actattaata cttctctgtt agacgattta
1981 cctgggaaat ccttcagcca gtaccttcaa agagaagggt acgactctgt gggagaagct
2041 acccttacac tcaatgagat tagagaaatt tacttaattt ggttggtgga tatttatcat
2101 aaaaaaccca atcagagagg cactaattgt cctaatgttg cgtggaaaaa gggttgtcaa
2161 gaatgggaac cagaggagtt ctctggttct aaagacgaat tagactttaa atttgctatt
2221 gttgattaca aacaacttac taaagtaggg ataactgtct acaaagaact gagttatagc
2281 aatgaccgtt tagctgaata tagagggaag aaaggaaacc ataaagttca gttcaagtat
2341 aaccctgagt gtatggcagt tatttgggtg ttggatgagg atatgaatga gtactttaca
2401 gttaatgcga ttgactacga atatgcaagt agagtatcac tttggcaaca taaatataac
2461 atgaaatatc aagcagaact aaattcagca gaatatgatg aggacaagga aattgatgca
2521 gaaataaaaa ttgaagaaat cgcagatcgt tcaattgtta agactaacaa aatcagagct
2581 cggaggcgtg gcgctaggca tcaagagaat agcgcaaggg ctaagtcaat cagtaatgcg
2641 aacccggcct cgatacaaaa acatgaagat gaaatcgtta gtgcagataa tgacgattgg
2701 gatattgatt atgtctgaga aatcgtcaaa tgagtgaaac gcgtgaggct cgtatatcaa
2761 gagctaaaag ggcatttgta tccacaccga gcgttaggaa aatcttgagt tacatggata
2821 gatgtagaga tctatcagac ctagagtctg agcctacatg catgatggtc tatggtgctt
2881 cgggtgtagg taaaacgacc gtcatcaaga aatacttaaa tcaaaacaga agagagtccg
2941 aagccggggg cgatataata ccggttttgc atattgagtt gccagacaat gcgaagccag
3001 tagatgcagc aagggaattg ctggttgaaa tgggtgaccc gctagcactt tatgaaactg
3061 acttagctag attgacgaaa agactgactg aattaatccc tgcggtcggc gtgaagctga
3121 ttattatcga tgagttccaa catttggtgg aagaaaggtc aaatcgggtt cttacccaag
3181 taggtaattg gctaaaaatg atacttaaca aaacgaaatg tccaattgtt atatttggta
3241 tgccatactc aaaagttgta ctgcaagcaa actcgcaact tcacgggcga ttttccattc
3301 aggttgaact gcgccccttt agctaccagg gaggtagagg tgtatttaaa acttttttgg
3361 aataccttga taaagcccta ccttttgaaa aacaggctgg cttagccaac gaaagtttgc
3421 agaaaaaatt gtatgcattc tctcagggaa acatgcgttc gttgagaaac cttatttatc
3481 aagcatctat cgaagcaatt gataatcagc atgaaacgat aaccgaagaa gatttcgttt
3541 ttgcatcgaa gttgacatcg ggcgataaac ccaactcatg gaaaaatcct tttgaggagg
3601 gtgttgaggt aacagaagat atgttacgac cgccaccaaa agatattggt tgggaggact
3661 atttgagaca ttcaaccccg agagtgagta aaccaggtag aaataaaaac tttttcgaat
3721 aacctaggct gctgccaccg ctgagcaata accatcttag tatattagtt aagtataaga
3781 aggagatata catatgtttt tgcaaagacc taaaccttac agcgatgaaa gtttagaaag
3841 tttctttatc cgagtggcta acaaaaatgg ctacggtgat gtccatcgct tcctagaagc
3901 cactaaacga ttccttcaag acattgacca taatggctat caaacctttc cgactgatat
3961 aactcggata aacccatact cagctaaaaa cagttccagc gcacgaactg cgtcattcct
4021 gaaacttgca caattgacat ttaatgaacc gccagagcta cttgggttgg caattaacag
4081 aacaaacatg aaatactcgc cgtcaactag cgcggttgtt agaggtgcag aagtctttcc
4141 tcgcagttta ctacggacgc actccatccc ctgctgtcct ttgtgtctgc gagaaaatgg
4201 ctacgcctcc tacctttggc actttcaggg gtacgaatac tgccacagcc ataacgtacc
4261 tttaattacc acttgtagct gtggtaagga gtttgactac cgagtatctg ggttaaaggg
4321 catttgctgc aaatgcaagg agcctatcac cttaaccagc agggagaacg gtcatgaggc
4381 agcgtgtact gtttcaaact ggcttgctgg ccatgaatct aaacctctgc caaatcttcc
4441 taaaagctac cgatggggtt tagttcattg gtggatgggt attaaagata gcgagttcga
4501 tcacttttcg ttcgttcaat ttttctcaaa ctggccaagg tcattccact cgataatcga
4561 agatgaagta gagttcaacc ttgagcatgc tgttgtcagc acgtctgaat tacgactaaa
4621 agatcttctt ggtcgattgt ttttcggttc aattcggtta cctgagcgga atcttcaaca
4681 caatatcatc cttggtgagc ttctctgcta tttagaaaat cgtttatggc aagacaaggg
4741 attaatcgcc aacctcaaaa tgaacgcgtt agaggcgact gtaatgttaa attgtagcct
4801 cgatcagatt gcatcaatgg ttgaacaacg catcttgaag ccaaatcgaa aaagcaagcc
4861 caacagccct cttgatgtta ccgattatct atttcatttc ggcgatattt tctgtctttg
4921 gttagctgag ttccaaagcg atgagtttaa ccgttcgttt tatgtgtcga ggtggtaaat
4981 gcaaactctg aaagaactaa tcgcatccaa tcctgacgac ttaacaactg aacttaagag
5041 agcatttcgt ccactcacac cgcatattgc aattgatggt aatgaacttg acgcactgac
5101 gatattagtc aatttaaccg ataagactga tgatcagaaa gacctgctcg atcgagccaa
5161 atgcaagcaa aaacttcgag atgaaaaatg gtgggctagc tgcataaatt gcgttaatta
5221 cagacaaagc cataacccaa aattcccgga tatacgttct gaaggcgtga ttcgaaccca
5281 agccctgggt gaattaccga gtttcctact ctcgtcctca aaaatcccac cataccattg
5341 gtcatatagc catgattcaa aatacgtcaa caaaagcgca ttcctcacca atgagttttg
5401 ttgggatggt gagatctcat gtttgggtga gcttcttaaa gatgcagatc acccactttg
5461 gaatactttg aaaaagttag gttgttctca aaaaacttgc aaagcaatgg caaaacaact
5521 agctgatatt actctcacga ctatcaatgt cacactcgca ccaaattacc tgactcaaat
5581 atctctcccc gatagtgata catcctacat ttcactctct cctgttgcat cgctatcgat
5641 gcaaagccac tttcatcaga ggctccaaga tgaaaatcgg catagcgcga taacgcggtt
5701 tagccgaact accaacatgg gggtcactgc gatgacatgt ggcggcgcat ttagaatgtt
5761 aaagtctggc gctaagtttt ccagcccccc tcatcaccga ttaaacagta aacgaagttg
5821 gttgacgtca gagcatgttc agtcattaaa acagtaccag cgcctcaaca aaagcctcat
5881 acctgaaaac tctcggattg cactccgtag aaaatacaaa atcgagcttc aaaatatggt
5941 cagatcttgg tttgcaatgc aagaccatac ccttgattcg aacatactta tccaacattt
6001 gaatcatgac ctatcttact taggagccac aaaacgtttt gcatacgatc cagcgatgac
6061 caagctcttt actgagcttt tgaaacgaga gttatcaaat tcaatcaata atggtgagca
6121 acacactaat ggatcgtttt tagtcctacc gaatatcagg gtttgtggcg caacagcttt
6181 aagctccccg gtaacggtgg ggattccatc acttacagct ttctttggct tcgttcacgc
6241 atttgaacgg aatataaatc gcaccacctc atcgtttcgt gttgaatcct ttgcgatatg
6301 cgtccatcaa ctacatgtcg agaagcgagg tttgacagca gagtttgtgg aaaaaggcga
6361 cgggactata tccgctcccg cgacccggga tgactggcag tgtgatgtcg tatttagcct
6421 tattttgaac accaactttg ctcaacatat tgaccaagat acgttagtta catcactacc
6481 aaagcgattg gctcggggtt cagcaaaaat tgcgattgat gactttaaac atatcaactc
6541 attctcgaca ttagaaacag cgatcgaatc tctgccaata gaagctggta ggtggttatc
6601 actttacgca cagtcaaaca ataatctaag tgatctatta gcagccatga cagaggacca
6661 tcagctcatg gcaagctgcg tcggttacca cttgttagaa gaacccaaag ataaaccaaa
6721 ctccctcaga ggttacaaac acgctatcgc cgagtgcatc attggactca ttaactcaat
6781 cacctttagc tcagagactg atcccaacac aatcttttgg tcgctaaaga actatcaaaa
6841 ctacctagtg gtacagccaa ggagtatcaa cgatgaaact accgacaaat ctagcctatg
6901 agcgctctat cgacccatca gatgtctgtt tttttgtcgt ctggcccgat gatagaaaaa
6961 cacctttaac ctacaattct cgtactctgc tcgggcaaat ggaagcggca tcattagcct
7021 atgatgtctc aggtcaacca ataaaaagtg ccaccgctga ggcgttagct caagggaacc
7081 ctcatcaagt tgatttctgc cacgttccat acggtgcgag tcatattgaa tgcagtttct
7141 ccgtttcgtt ttcttctgaa ctacgtcaac catataagtg taactcaagc aaagttaaac
7201 aaacgctagt gcaattagtc gagctctacg aaacgaaaat cggctggact gagctagcaa
7261 cccgatattt gatgaacatt tgcaacggta aatggctgtg gaaaaatacc cgtaaagcct
7321 attgctggaa cattgtactt acaccttggc cttggaacgg ggaaaaggtt ggatttgaag
7381 atattcgtac taactacacc tcacggcaag actttaaaaa taataaaaat tggtctgcta
7441 tagttgaaat gatcaaaacc gcattttcta gtactgatgg gctggcgata tttgaagtca
7501 gggccacctt gcacttgcca acgaatgcta tggtgcggcc aagccaagtt ttcacagaaa
7561 aagaaagtgg cagtaaaagt aaatctaaaa ctcaaaacag tcgagttttt cagagtacaa
7621 ctattgatgg tgaacgatcg ccaatactag gggcctttaa aacgggagca gctattgcaa
7681 ccattgacga ctggtatcct gaagccactg agccactaag ggtcggacgg tttggggttc
7741 atcgcgaaga tgtcacttgc taccgtcatc cgtctaccgg aaaagatttt ttctcgatat
7801 tacaacaagc agagcactat attgaagtgt tgagcgccaa caaaactccc gctcaagaaa
7861 ctatcaacga catgcacttt ttaatggcta acctgattaa gggtgggatg ttccagcata
7921 aaggagactg actgtgaaat ggtattataa gacaatcacc tttctgccag agttgtgcaa
7981 caacgagtca ctggctgcaa agtgtctccg cgttctgcat ggatttaact atcagtatga
8041 gacacgaaat atcggcgttt catttccgct ttggtgtgat gcaacggttg gaaaaaagat
8101 ttcatttgtc agcaagaaca agatagaact cgacttacta cttaaacaac actatttcgt
8161 ccaaatggaa caacttcaat actttcatat atccaacact gttctcgtcc cagaagattg
8221 tacatacgtt tcctttagac gctgtcaatc tatagataag ctcacagcag cagggctggc
8281 aaggaaaatc agacgcctgg agaaacgtgc tctatctaga ggcgagcaat ttgacccatc
8341 atcttttgct caaaaagagc atactgcaat agcgcactac cactcacttg gggagtccag
8401 caaacagacg aaccgcaact ttcgactcaa tatcaggatg ctctcggagc aaccccgtga
8461 ggggaactcg atttttagta gctatggctt atcaaattca gaaaactcgt ttcagcctgt
8521 acccttaatc tgacctaggc tgctgccacc ggtgaactgc cgagtaggta gctgataacg
8581 agacctcgtt tacctatcgg tctcgtgaac tgccgagtag gtagctgata acccaaacca
8641 gatgtcaaca cagctactgt tgatgcaacc ataaagtgat atttaataat tatttataat
8701 cagcaactta accacaaaac aaccatatat tgatatctca caaaacaacc ataagttgat
8761 atttttcagc caggggtccc caataattac gatttaaatt tgacataagc ctgttcggtt
8821 cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
8881 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta
8941 cagcctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
9001 ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc
9061 taaaacaaag ttaggtggct caagtatggg catcattcgc acgtgtaggc tcggccctga
9121 ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gtgacgtagc
9181 cacctactcc caacatcagc cggattccga ttacctcggg aacttgctcc gtagtaagac
9241 attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt
9301 tctgcccaag tttgagcagc cgcgtagtga aatctatatc tatgatctcg cagtctccgg
9361 agagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa
9421 cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc
9481 tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac
9541 cgccacctaa caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa
9601 attggacaac ggtccgcgcg ttgtcctttt ccgctgcata accctgcttc ggggtcatta
9661 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
9721 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gaaagtgaaa cgtgatttca
9781 tgcgtcattt tgaacatttt gtaaatctta tttaataatg tgtgcggcaa ttcacattta
9841 atttatgaat gttttcttaa catcgcggca actcaagaaa cggcaggttc ggatcttagc
9901 tactagagaa agaggagaaa tactagatgc gtaaaggcga ggagctgttc actggtgtcg
9961 tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg
10021 agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta
10081 aactgccggt tccttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg
10141 ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct
10201 atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag
10261 tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag
10321 aggacggcaa tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca
10381 tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg
10441 aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc
10501 ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga
10561 acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg
10621 gtatggatga actgtacaaa tgaccaggca tcaaataaaa cgaaaggctc agtcgaaaga
10681 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
10741 ctcaccttcg ggtgggcctt tctgcgttta tagctgtaat acgactcact ataggggata
10801 agagacggct caacgcccgt ctcacagcgc aatatcaatt tatgggtgtg ataattatca
10861 atttatgggt gtaattatca ttttatggtt gtatcaacaa ggaggacttt ggcaggtgca
10921 gcacaaaagg aaactcaccg cgatcgccat tactcgcatc agcctgcagg attcttgctt
10981 atcggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
11041 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
11101 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
11161 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
11221 gaaaagaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
11281 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
11341 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
11401 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
11461 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
11521 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
11581 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
11641 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
11701 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
11761 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
11821 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
11881 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
11941 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
12001 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
12061 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg cggtatcatt
12121 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
12181 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
12241 cattggtaac tgtcagaccc ggccacgatg cgtccggcgt agaggatctg aagatcagca
12301 gttcaacctg ttgatagtac gtactaagct ctcatgtttc acgtactaag ctctcatgtt
12361 taacgtacta agctctcatg tttaacgaac taaaccctca tggctaacgt actaagctct
12421 catggctaac gtactaagct ctcatgtttc acgtactaag ctctcatgtt tgaacaataa
12481 aattaatata aatcagcaac ttaaatagcc tctaaggttt taagttttat aagaaaaaaa
12541 agaatatata aggcttttaa agcttttaag gtttaacggt tgtggacaac aagccaggga
12601 tgtaacgcac tgagaagccc ttagagcctc tcaaagcaat tttgagtgac
//
