pEF6/V5-His C

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价格：元

联系方式：I47-825O-882O

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pEF6/V5-His C载体 pEF6/V5-His C质粒基本信息

出品公司:	Invitrigen
载体名称:	pEF6/V5-His C
质粒类型:	哺乳细胞表达载体
高拷贝/低拷贝:	高拷贝
启动子:	EF-1α
克隆方法:	多克隆位点，限制性内切酶
载体大小:
5' 测序引物及序列:	T7 Forward：TAATACGACTCACTATAGGG
3' 测序引物及序列:	--
载体标签:	His Tag (6x) ; V5 Epitope Tag
载体抗性:	氨苄青霉素（Ampicillin）
筛选标记:	Blasticidin
备注:	易于克隆到其它载体中去；高表达水平。
产品目录号:	V961-20
稳定性:	瞬表达
组成型:	组成型表达
病毒/非病毒:	非病毒

pEF6/V5-His C质粒图谱 pEF6/V5-His C载体图谱和pEF6/V5-His C载体序列 pEF6/V5-His C质粒序列多克隆位点信息

pEF6-V5-His C载体图谱

pEF6/V5-His C质粒 pEF6/V5-His C载体简介

pEF6/V5-His C

All pEF or pUB vectors contain a strong promoter for high-level expression 
in mammalian cells, a choice of selection marker for generating stable 
cell lines, and an epitope tag for easy detection with a monoclonal 
antibody and rapid purification on nickel-chelating resin. Each 
vector is available in three reading frames to simplify cloning 
in-frame with the fusion tag.

pEF6/V5-His C质粒序列 pEF6/V5-His C载体序列

AATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAA
TAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTC
CCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCC
TGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACC
GACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATA
CGCGTTGACATTGATTATTGACTAGGCTTTTGCAAAAAGCTTTGCAAAGATGGATAAAGTTTTAAACAGA
GAGGAATCTTTGCAGCTAATGGACCTTCTAGGTCTTGAAAGGAGTGGGAATTGGCTCCGGTGCCCGTCAG
TGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCT
AGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGG
GGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACA
CAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAA
TTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTC
GAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGC
CGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATT
TTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACAC
TGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGG
CGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGC
CTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTG
AGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAG
CGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCA
CGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTG
GGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGG
CACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGA
CAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAGGAATTAGCTTGGTACTAATACGACTCAC
TATAGGGAGACCCAAGCTGGCTAGGTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGTGGAA
TTCTGCAGATATCCAGCACAGTGGCGGCCGCTCGAGGTCACCCATTCGAAGGTAAGCCTATCCCTAACCC
TCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCATCACCATCACCATTGAGTTTAAACCCGCTGATCA
GCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGG
AAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCA
TTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCT
GGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACG
CGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAG
CGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAA
GCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTG
ATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTC
CACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTT
GATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACG
CGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGT
ATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAA
GTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCT
AACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAG
GCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA
AGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAGCACGTGTTGACAATTAATCATCGGCATAG
TATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGCCAAGCCTTTGTCTCAAGAAGAAT
CCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAG
CGCAGCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGT
GCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCG
GAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAGGTGCTTCTCGATCTGCATCCTGG
GATCAAAGCCATAGTGAAGGACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCT
GGTTATGTGTGGGAGGGCTAAGCACTTCGTGGCCGAGGAGCAGGACTGACACGTGCTACGAGATTTCGAT
TCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCC
AGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAA
ATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCC
AAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGT
CATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAA
GTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTC
CAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTA
TTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATC
AGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCA
AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCAT
CACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCC
TTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC
AAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAG
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTT
GGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGA
AGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC
ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAA
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTG
TCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCA
TCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACC
AGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG
TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGC
ATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTA
CATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTT
GGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGA
TGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT
CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAA
ACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGT
GCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAA
ATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTC

pEF6/V5-His C载体图谱 pEF6/V5-His C质粒图谱pdf版和pEF6/V5-His C载体序列 pEF6/V5-His C质粒序列等相关资料下载

pEF6/V5-His C质粒 pEF6/V5-His C载体应用举例

上一篇：pEF6/myc-His A
下一篇：pEF6/V5-His TOPO TA

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG	pGFP22
pDB262	pSP65-F1+
p19D+	pYES2-EGFP
pDNAPolbeta_TA	pGL4.18[luc2P/Neo]
pENTR-L1pLaclacZaL4	pHBT-GFP-NOS
pBBR1MCS2-Tac-mCherry	pAAV-TRE-CAG-Fluc
pLVX-AmCyan1-N1	pcDNA3.1-mRFP
pHT01	pEYFP-Mem
pPM408	pcDNA3.1/Zeo(-)
pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG	pcDNA6.2/nTC-Tag-DEST
pSuper.Retro.Neo	pUC19
pcDNA4/TO/Myc-His/LacZ	pAAVLINK-EF1a-HA-5'-DIP2C
pIEX-1	pLC4-14
pBTB-5	pSwitch
pFastBac1-His-C	pCEP4-DsRed2
pSEAP2-Basic	pBARMTE1
pQE-16	pHIL-D2
pYC25	pNov.aro-bgl1-640
pAAV-EFS-DIO-GRAB_OT1.0	Pgex-HRV3C
pGL4.21[luc2P/Puro]	pcDNA3.1-CNTRFv1-HA-His
pGEX-6p-1-hsG3BP2b	pBad33
pMMB66EH	pRetroX-Tight-Hyg
pAAVLINK-EF1a-HA-5-RALGAPB	pAMβ1
pGEX-6p-1-AT5G48650	pWY113 pET28a_AtmtE2
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG	pIEx/Bac-3
pAP1(PMA)-TA-Luc	pDEST8
pFR-Luc	ppyrG
pBR327	pL-brevisGH1-TA
pVK101	pQLinkN
pS-hydro_TA	pENTR5'/CMVp
pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG	YEp620
pAAVLINK-EF1a-5'-PXDN	pMCS-Green-Renilla-Luc
pDM4	pHY300PLK
pBluescript II KS(-)	pCRE-DD-AmCyan1
pFN10A (ACT)	YEp24
pUTK21	pET-53-DEST
pMAL-p5x	pHOOK1
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG	pIEX-3
pHR31	pCEP4-Flag-N
pAAV-hSyn-Cepheid1s-WPRE	pCDEF-HA-Ub-K63R
pCold-SUMO	pIZT/V5-His
pCRII-TOPO/22-1	pAAV-CAG-NES-YTnC
pRI101-AN	pBABE-HAII
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG	pcDNA3.1+N-DYK-P2A
pShuttle-CMV	pENTR U6
pIRESpuro3	pAAVLINK-EF1a-HA-5'-ITSN1
p1JI008	pAX01-ComK
pHisB-Sumo-NeMeHg	pSW510
YEp16	FUW-tetO-hOCT4
pET28a-mOrange(M163A)	pLenti6/V5-GW/lacZ

pT7-SpCas9_sgRNA-site1 (RTW443)	pcDNA3.1 SIRT6_H133Y
pSI-Target-AID-NG	pJRH-1179 U6-reci Gag-Cas9 v2
mAzurite-8xbiRhoBAST-24xMS2v6	pAc-sgRNA-Cas9
pLminP_Luc2P_RE4	pcDNA3 Connexin43-GFP-APEX2
BB3cK_pGAP_23*_pTEF_Cas9	p6XHis-T7(P266L)
pcDNA3.1-ERBB4	SIRT6 Flag
pXR002:EF1a-dCasRx-2A-EGFP	pCDNA3 Flag p38 alpha
pCRISPR-cBEST	pRK5-HA-Ubiquitin-K29R
pPB-AMBRA1-3xFLAG	pCMV-HA-JMJD3
pRK5-HA-SH3BP4 W92A	pBiFC-VN155(I152L)
pAKTaq	pLIC-SM-HA
pBIG1a	pGHYB
GAG-CRErec	pEG302 22aa SunTag NtDRMcd (no NLS) g4+g
pCAG-FAST-p10	pAAV2/9n
pCI-neo.mCherry-NEDD4	Fuw-dCas9-Dnmt3a_IM
LentiGuide_DKO	pMIH-TOMM20-Halo
murine TCR OTI-2A.pMIG II	pKAR2-Br512
pEF1a-Usp9x_CD-mCherry	pINDUCER21-puro_Gateway-3xFLAG
pCDH-DCX-TurboID-V5-puro	EGFP-LC3
pEBTet_NAD-Snifit-mito	GFP-MAP1B
shYAP1	phage UbiC G3BP1-SNAP
pCMT-flp	MG1655 ΔendA ΔrecA (DE3)
pQCXIP-GFP-LacR	pQUAST>stop>mCD8-GFP
pLTR-RD114A	pcDNA3.1-TYMS
pNG9	PX551
pBK092	pHIV-Luciferase
Double UP mNeon to mScarlet	mARG1 cassette 3
CD109-bio-His	pGEX6p-1-BCCP-PupE
pcDNA3.1_AktPH-mCherry	AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-
pLenti-MP2	pCITEp65
pTM1_VP30_ZEBOV	pBLO 64.1
pET16B.Pfu	pGP-CMV-Gcamp6m
pAAVS1-PC-GCaMP6f	pscAAV-CAG-GFP
pTA-HUH	PA-GFPmt
pDriver	GW1-Peredox-mCherry-NLS
pDawn-sfGFP	pCMV-mA1-BE3
MP6	LeGO-iG
pcDNA3.1(+) CircRNA Mini Vector	pCutamp
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGre	pHCMV_DUGV_GP
pT7-hMLH1dn for IVT	AdEasier-1 cells (strain)
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W	pKO (Hyg)
pCS2+HyPer7-NLS	pET28GST-LIC
pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3	pEGFP-C1 F-tractin-EGFP
PB533_15F11-sfGFP	pMXs-hSOX2
BCR/ABL P190 transgenic construct	pcDNA5-T/O-Ds1-mCherry
pRH2502	CAMK2D gRNA (BRDN0001146112)
pX330_SpCas9_USP7 Exon 3 gRNA	pShuttle-CMV-GFP U6promoter
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA	Myc tag WT PTB
pQCasTns(Vch)-entry(BsaI)	pBSK
pMR-A-3	pCI-6.20

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pEF6/V5-His C

买家导航

pEF6/V5-His C载体 pEF6/V5-His C质粒基本信息

pEF6/V5-His C质粒图谱 pEF6/V5-His C载体图谱和pEF6/V5-His C载体序列 pEF6/V5-His C质粒序列多克隆位点信息

pEF6/V5-His C质粒 pEF6/V5-His C载体简介

pEF6/V5-His C质粒序列 pEF6/V5-His C载体序列

pEF6/V5-His C载体图谱 pEF6/V5-His C质粒图谱pdf版和pEF6/V5-His C载体序列 pEF6/V5-His C质粒序列等相关资料下载

pEF6/V5-His C质粒 pEF6/V5-His C载体应用举例

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