| 质粒名称: | MSP832 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 98792 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pSC101 |
| 载体出品公司: | ATCC |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 合成生物 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | proB-lysozyme-ggs-HPLVGHM-ggs-T7RNAP(Q649S)//T7pro-gIII-lux |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | MSP832 was a gift from David Liu (Addgene plasmid # 98792 ; http://n2t.net/addgene:98792 ; RRID:Addgene_98792) |
| 参考文献部分: | Phage-assisted continuous evolution of proteases with altered substrate specificity. Packer MS, Rees HA, Liu DR. Nat Commun. 2017 Oct 16;8(1):956. doi: 10.1038/s41467-017-01055-9. 10.1038/s41467-017-01055-9 [pii] PubMed 29038472 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MSP832 10017 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Accessory Plasmid for PACE.
ACCESSION .
VERSION .
KEYWORDS MSP832
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10017)
AUTHORS Packer MS, Rees HA, Liu DR
TITLE Phage-assisted continuous evolution of proteases with altered
substrate specificity.
JOURNAL Nat Commun. 2017 Oct 16;8(1):956. doi: 10.1038/s41467-017-01055-9.
PUBMED 29038472
REFERENCE 2 (bases 1 to 10017)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10017
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(1..951)
/codon_start=1
/gene="rep101"
/product="RepA protein needed for replication with the
pSC101 origin"
/label=Rep101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 999..1221
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
primer_bind 1785..1804
/label=T7
/note="T7 promoter, forward primer"
promoter 1785..1803
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3259..4338
/codon_start=1
/gene="luxA"
/product="LuxA luciferase subunit"
/label=LuxA
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
KLFQSDVMPFLKEKQRSLLY"
CDS 4402..5382
/codon_start=1
/gene="luxB"
/product="LuxB luciferase subunit"
/label=LuxB
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
CDS complement(8215..8664)
/codon_start=1
/product="lysozyme from bacteriophage T7"
/label=T7 lysozyme
/note="inhibitor of T7 RNA polymerase"
/translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
RSLLVTLLAKYEGAVLRAHHEVAPKASPSFDLKRWWEKNELVTSDR"
terminator 8849..8895
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(8946..9806)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9569..9588
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9807..9911)
/gene="bla"
/label=AmpR promoter
primer_bind 9979..9997
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
1741 aagactgggc ctttcgtttt gctgaggaga cttagggacc ctactaatac gactcactat
1801 agggagaaag aaggagcgac attgctccgt gtattcactc gttggaatga atacacagtg
1861 cagtgtttat tctgttattt atgccaaaaa taaaggccac tatcaggcag ctttgttgtt
1921 ctgtttacca agttcaggag gtaactcata agaaagacct gcaggtgcag taaaggaaaa
1981 aaaaatgaaa aaattattat tcgcaattcc tttagttgtt cctttctatt ctcactccgc
2041 tgaaactgtt gaaagttgtt tagcaaaacc ccatacagaa aattcattta ctaacgtctg
2101 gaaagacgac aaaactttag atcgttacgc taactatgag ggctgtctgt ggaatgctac
2161 aggcgttgta gtttgtactg gtgacgaaac tcagtgttac ggtacatggg ttcctattgg
2221 gcttgctatc cctgaaaatg agggtggtgg ctctgagggt ggcggttctg agggtggcgg
2281 ttctgagggt ggcggtacta aacctcctga gtacggtgat acacctattc cgggctatac
2341 ttatatcaac cctctcgacg gcacttatcc gcctggtact gagcaaaacc ccgctaatcc
2401 taatccttct cttgaggagt ctcagcctct taatactttc atgtttcaga ataataggtt
2461 ccgaaatagg cagggggcat taactgttta tacgggcact gttactcaag gcactgaccc
2521 cgttaaaact tattaccagt acactcctgt atcatcaaaa gccatgtatg acgcttactg
2581 gaacggtaaa ttcagagact gcgctttcca ttctggcttt aatgaggatc cattcgtttg
2641 tgaatatcaa ggccaatcgt ctgacctgcc tcaacctcct gtcaatgctg gcggcggctc
2701 tggtggtggt tctggtggcg gctctgaggg tggtggctct gagggtggcg gttctgaggg
2761 tggcggctct gagggaggcg gttccggtgg tggctctggt tccggtgatt ttgattatga
2821 aaagatggca aacgctaata agggggctat gaccgaaaat gccgatgaaa acgcgctaca
2881 gtctgacgct aaaggcaaac ttgattctgt cgctactgat tacggtgctg ctatcgatgg
2941 tttcattggt gacgtttccg gccttgctaa tggtaatggt gctactggtg attttgctgg
3001 ctctaattcc caaatggctc aagtcggtga cggtgataat tcacctttaa tgaataattt
3061 ccgtcaatat ttaccttccc tccctcaatc ggttgaatgt cgcccttttg tctttggcgc
3121 tggtaaacct tacgagttca gtatcgactg cgataagatc aacctgttcc gcggtgtctt
3181 tgcgtttctt ttatatgttg ccacctttat gtatgtattt tctacgtttg ctaacatact
3241 gcgtaataag gagtcttaat gaaatttgga aactttttgc ttacatacca acctccccaa
3301 ttttcccaaa cagaggtaat gaaacgtttg gttaaattag gtcgcatctc tgaggagtgt
3361 ggttttgata ccgtatggtt actggagcat catttcacgg agtttggttt gcttggtaac
3421 ccttatgtcg ctgctgcata tttacttggc gcgactaaaa aattgaatgt aggaactgcc
3481 gctattgttc ttcccacagc ccatccagta cgccaacttg aagatgtgaa tttattggat
3541 caaatgtcaa aaggacgatt tcggtttggt atttgccgag ggctttacaa caaggacttt
3601 cgcgtattcg gcacagatat gaataacagt cgcgccttag cggaatgctg gtacgggctg
3661 ataaagaatg gcatgacaga gggatatatg gaagctgata atgaacatat caagttccat
3721 aaggtaaaag taaaccccgc ggcgtatagc agaggtggcg caccggttta tgtggtggct
3781 gaatcagctt cgacgactga gtgggctgct caatttggcc taccgatgat attaagttgg
3841 attataaata ctaacgaaaa gaaagcacaa cttgagcttt ataatgaagt ggctcaagaa
3901 tatgggcacg atattcataa tatcgaccat tgcttatcat atataacatc tgtagatcat
3961 gactcaatta aagcgaaaga gatttgccgg aaatttctgg ggcattggta tgattcttat
4021 gtgaatgcta cgactatttt tgatgattca gaccaaacaa gaggttatga tttcaataaa
4081 gggcagtggc gtgactttgt attaaaagga cataaagata ctaatcgccg tattgattac
4141 agttacgaaa tcaatcccgt gggaacgccg caggaatgta ttgacataat tcaaaaagac
4201 attgatgcta caggaatatc aaatatttgt tgtggatttg aagctaatgg aacagtagac
4261 gaaattattg cttccatgaa gctcttccag tctgatgtca tgccatttct taaagaaaaa
4321 caacgttcgc tattatatta tggcggtggc ggtagcggcg gtggcggtag cggcggtggc
4381 ggtagcggcg gtggcggtag caaatttgga ttgttcttcc ttaacttcat caattcaaca
4441 actgttcaag aacagagtat agttcgcatg caggaaataa cggagtatgt tgataagttg
4501 aattttgaac agattttagt gtatgaaaat catttttcag ataatggtgt tgtcggcgct
4561 cctctgactg tttctggttt tctgctcggt ttaacagaga aaattaaaat tggttcatta
4621 aatcacatca ttacaactca tcatcctgtc cgcatagcgg aggaagcttg cttattggat
4681 cagttaagtg aagggagatt tattttaggg tttagtgatt gcgaaaaaaa agatgaaatg
4741 cattttttta atcgcccggt tgaatatcaa cagcaactat ttgaagagtg ttatgaaatc
4801 attaacgatg ctttaacaac aggctattgt aatccagata acgattttta tagcttccct
4861 aaaatatctg taaatcccca tgcttatacg ccaggcggac ctcggaaata tgtaacagca
4921 accagtcatc atattgttga gtgggcggcc aaaaaaggta ttcctctcat ctttaagtgg
4981 gatgattcta atgatgttag atatgaatat gctgaaagat ataaagccgt tgcggataaa
5041 tatgacgttg acctatcaga gatagaccat cagttaatga tattagttaa ctataacgaa
5101 gatagtaata aagctaaaca agagacgcgt gcatttatta gtgattatgt tcttgaaatg
5161 caccctaatg aaaatttcga aaataaactt gaagaaataa ttgcagaaaa cgctgtcgga
5221 aattatacgg agtgtataac tgcggctaag ttggcaattg aaaagtgtgg tgcgaaaagt
5281 gtattgctgt cctttgaacc aatgaatgat ttgatgagcc aaaaaaatgt aatcaatatt
5341 gttgatgata atattaagaa gtaccacacg gaatatacct aaacttaatt aacggcactc
5401 ctcagcaaat ataatgaccc tcttgataac ccaagagggc attttttaat gcccatggcg
5461 tcatgttgaa aatctccatt acgcgaacgc gaagtccgac tctaagatgt cacggaggtt
5521 caagttacct ttagccggaa gtgctggcat tttgtccaat tgagactcgt gcaactggtc
5581 agcgaactgg tcgtagaaat cagccagtac atcacaagac tcatatgtgt caaccatagt
5641 ttcgcgcact gctttgaaca ggttcgcagc gtcagccgga atggtaccga aggagtcgtg
5701 aatcagtgca aaagattcga ttccgtactt ctcgtgtgcc cacactacag tcttacgaag
5761 gtggctaccg tcttggctgt gtacaaagtt aggagcgata ccagactcct gtttgtgtgc
5821 atcaatctcg ctatctttgt tggtgttaat ggtaggctgt aagcggaact gaccgaggaa
5881 catcaggttc aagcgcgtct gaataggctt cttgtattcc tgccacacag ggaaaccatc
5941 aggagttacc caatgcacag cgcaacgctt gcgaagaatc tctccagtct tcttatcttt
6001 gacctcagca gccagcagct tagcagcaga cttaagccag ttcattgctt caaccgcagc
6061 taccaccgtc acgctcacag attcccaaat cagcttagcc atgtatccag cagcctgatt
6121 cggctgagtg aacatcagac ccttgccgga atcaatagct ggctgaatgg tatcttccag
6181 cactgattga cggaagccga actctttgga cccgtaagcc agcgtcatga ctgaacgctt
6241 agtcacactg cgagtaacac cgtaagccag ccattgacca gccagtgcct tagtgcccag
6301 cttgactttc tcagagattt caccagtgtt ctcatcggtc acggtaacta cttcgttatc
6361 ggtcccattg attgcgtctg cttgtagaat ctcgttgact ttcttagcaa caatcccgta
6421 gatgtcctga acggtttcac taggaagcaa gttaaccgcg cgaccaccta cctcatctcg
6481 gagcatcgcg gagaagtgct ggatgccaga gcaagacccg tcaaacgcca gcggaaggga
6541 gcagttatag ctcaggccgt ggtgctgtac cccagcgtac tcaaagcaga acgcaaggaa
6601 gcagaacgga gaatcttgct cagcccacca agtgttctcc agtggagact tagcgcaagc
6661 catgatgttc tcgtggtttt cctcaatgaa cttgatgcgc tcagggaacg gaaccttatc
6721 gacacccgca cagtttgcac cgtggatttt cagccagtag taaccttcct taccgattgg
6781 tttacctttc gccagcgtaa gcagtccttt ggtcatatcg ttaccttgcg ggttgaacat
6841 tgacacagcg taaacacgac cgcgccagtc catgttgtaa gggaaccaga tggccttatg
6901 gttagcaaac ttattggctt gctcaagcat gaactcaagg ctgatacggc gagacttgcg
6961 agccttgtcc ttgcggtaca cagcagcggc agcacgtttc cacgcggtga gagcctcagg
7021 attcatgtcg atgtcttccg gtttcatcgg gagttcttca cgctcaatcg cagggatgtc
7081 ctcgaccgga caatgcttcc acttggtgat tacgttggcg accgctagga ctttcttgtt
7141 gattttccat gcggtgtttt gcgcaatgtt aatcgctttg tacacctcag gcatgtaaac
7201 gtcttcgtag cgcatcagtg ctttcttact gtgagtacgc accagcgcca gaggacgacg
7261 accgttagcc caatagccac caccagtaat gccagtccac ggcttaggag gaactacgca
7321 aggttggaac atcggagaga tgccagccag cgcacctgca cgggttgcga tagcctcagc
7381 gtattcaggt gcgagttcga tagtctcaga gtcttgacct actacgccag cattttggcg
7441 gtgtaagcta accattccgg ttgactcaat gagcatctcg atgcagcgta ctcctacatg
7501 aatagagtct tccttatgcc acgaagacca cgcctcgcca ccgagtagac ccttagagag
7561 catgtcagcc tcgacaactt gcataaatgc tttcttgtag acgtgcccta cgcgcttgtt
7621 gagttgttcc tcaacgtttt tcttgaagtg cttagcttca aggtcacgga tacgaccgaa
7681 gcgagcctcg tcctcaatgg cccgaccgat tgcgcttgct acagcctgaa cggttgtatt
7741 gtcagcactg gttaggcaag ccagagtggt cttaattgtg atgtacgcta cggcttccgg
7801 cttgatttct tgcaggaact ggaaggctgt cgggcgcttg ccgcgcttag ctttcacttc
7861 ctcaaaccag tcgttgatgc gtgcaatcat cttagggagt agggtagtga tgagaggctt
7921 agcggcagcg ttatccgcaa cctcaccagc tttaagttga cgctcaaaca tcttgcggaa
7981 gcgtgcttca cccatctcgt aagactcatg ctcaagggcc aactgttcgc gagctaaacg
8041 ctcaccgtaa tggtcagcca gagtgttgaa cgggatagca gccagttcga tgtcagagaa
8101 gtcgttctta gcgatgttaa tcgtgttacc gcccgctcca ctgcctgcgc taccgcctgc
8161 catgtgacca accagggggt gacccgcacc accactcgca ccaccgccgc taccacggtc
8221 agaagtgacc agttcgttct tctcccacca acgcttaagg tcgaacgaag gggaagcctt
8281 cggcgccacc tcatgatggg cgcgaagcac agcgccttcg tacttagcca gcagtgtgac
8341 aagcagtgag cgaagggatt gcatttgggc tggcgtaaag ttagcgtcga acttaccttt
8401 atcgtcgata ccaccaacaa ggcagacgcc gatagagttg tggttgtaac ccttagcgtg
8461 agagcctaca gccatctcat ctcgtcctgc ctccacagta ccgtctcgct tgatgataaa
8521 gtggtatccc acatcgagcc aaccctgctc tttgtgccac tggcgaatct cacggacacc
8581 aacattctga cttggcttgg tagccgagca gtgaacaaag attgcgtcag tagattcacg
8641 ttgtttaaac tgtacacgag ccattttttt tttcctttcg aggaaggaac tctagtaaaa
8701 gttaaacaaa attatttgta gagggaaacc gttgtggtct ccctgaatat atattacgag
8761 ccttatgcat gcccgtaaag ttatccagca accactcata gacctagggc agcagatagg
8821 gacgacgtgg tgttagctgt gctctagaat aaaacgaaag gcccagtctt tcgactgagc
8881 ctttcgtttt atttgaccgg aaatcagaat tggttaattg gttgtaacac tggcagagca
8941 ttacgttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
9001 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
9061 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
9121 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
9181 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
9241 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
9301 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
9361 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
9421 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
9481 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
9541 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
9601 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
9661 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
9721 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
9781 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
9841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
9901 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
9961 atgacattaa cctataaaaa taggcgtatc acgaggccct taggccaaat aggccgt
//
