| 质粒名称: | L-HBsAg |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 103011 |
| 存储实验室: | Wang-Shick Ryu |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 6608 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HBV Large envelope protein |
| 物种来源: | Hepatitis B virus |
| 插入大小: | 1170 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | L-HBsAg was a gift from Wang-Shick Ryu (Addgene plasmid # 103011 ; http://n2t.net/addgene:103011 ; RRID:Addgene_103011) |
| 参考文献部分: | Hepatitis B virus polymerase blocks pattern recognition receptor signaling via interaction with DDX3: implications for immune evasion. Wang H, Ryu WS. PLoS Pathog. 2010 Jul 15;6(7):e1000986. doi: 10.1371/journal.ppat.1000986. 10.1371/journal.ppat.1000986 PubMed 20657822 |
买家导航
LHBsAg载体基本信息
LHBsAg载体图谱质粒图谱和多克隆位点信息
LHBsAg载体简介
LHBsAg载体序列
LOCUS L-HBsAg 6607 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses HBV Large envelope glycoprotein in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS L-HBsAg
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6607)
AUTHORS Wang H, Ryu WS
TITLE Hepatitis B virus polymerase blocks pattern recognition receptor
signaling via interaction with DDX3: implications for immune
evasion.
JOURNAL PLoS Pathog. 2010 Jul 15;6(7):e1000986. doi:
10.1371/journal.ppat.1000986.
PUBMED 20657822
REFERENCE 2 (bases 1 to 6607)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6607
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1280..2125
/codon_start=1
/product="middle (pre-S2/S) envelope protein of hepatitis B
virus"
/label=HBsAg (pre-S2/S)
/note="hepatitis B surface antigen"
/translation="MQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSI
FSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCL
GQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCP
LIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWAS
ARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI
"
promoter complement(2160..2178)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2161..2178)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(2198..2215)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2204..2428
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2474..2902
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2561..2580)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2771..2792
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2911..2931)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2916..3245
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3096..3231
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3158..3177
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3312..4106
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3366..3385)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3976..3995
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4280..4401
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4317..4336)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4371..4390
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4450..4466)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4450..4466)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4463..4485)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4474..4490
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4498..4528)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4543..4564
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4681..4698)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4852..5440)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4932..4951)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5611..6471)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6234..6253
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6472..6576)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atcccatggg
961 gcagaatctt tccaccagca atcctctggg attctttccc gaccaccagt tggatccagc
1021 cttcagagca aacaccgcaa atccagattg ggacttcaat cccaacaagg acacctggcc
1081 agacgccaac aaggtaggag ctggagcatt cgggctgggt ttcaccccac cgcacggagg
1141 ccttttgggg tggagccctc aggctcaggg catactacaa actttgccag caaatccgcc
1201 tcctgcctcc accaatcgcc agtcaggaag gcagcctacc ccgctgtctc cacctttgag
1261 aaacactcat cctcaggcca tgcagtggaa ttccacaacc ttccaccaaa ctctgcaaga
1321 tcccagagtg agaggcctgt atttccctgc tggtggctcc agttcaggaa cagtaaaccc
1381 tgttctgact actgcctctc ccttatcgtc aatcttctcg aggattgggg accctgcgct
1441 gaacatggag aacatcacat caggattcct aggacccctt ctcgtgttac aggcggggtt
1501 tttcttgttg acaagaatcc tcacaatacc gcagagtcta gactcgtggt ggacttctct
1561 caattttcta gggggaacta ccgtgtgtct tggccaaaat tcgcagtccc caacctccaa
1621 tcactcacca acctcttgtc ctccaacttg tcctggttat cgctggatgt gtctgcggcg
1681 ttttatcatc ttcctcttca tcctgctgct atgcctcatc ttcttgttgg ttcttctgga
1741 ctatcaaggt atgttgcccg tttgtcctct aattccagga tcctcaacaa ccagcacggg
1801 accatgccgg acctgcatga ctactgctca aggaacctct atgtatccct cctgttgctg
1861 taccaaacct tcggacggaa attgcacctg tattcccatc ccatcatcct gggctttcgg
1921 aaaattccta tgggagtggg cctcagcccg tttctcctgg ctcagtttac tagtgccatt
1981 tgttcagtgg ttcgtagggc tttcccccac tgtttggctt tcagttatat ggatgatgtg
2041 gtattggggg ccaagtctgt acagcatctt gagtcccttt ttaccgctgt taccaatttt
2101 cttttgtctt tgggtataca tttaagcggc cgctcgagca tgcatctaga gggccctatt
2161 ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
2221 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
2281 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
2341 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
2401 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
2461 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
2521 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
2581 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
2641 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
2701 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
2761 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
2821 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
2881 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
2941 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
3001 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
3061 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
3121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
3181 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
3241 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
3301 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
3361 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
3421 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
3481 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
3541 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
3601 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
3661 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
3721 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
3781 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
3841 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
3901 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
3961 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
4021 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
4081 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
4141 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
4201 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
4261 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
4321 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
4381 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
4441 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
4501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
4561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
4621 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
4681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
4741 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
4801 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
4861 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
4921 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
4981 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
5041 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
5101 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
5161 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
5221 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
5281 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
5341 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
5401 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
5461 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
5521 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
5581 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
5641 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
5701 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
5761 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
5821 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
5881 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
5941 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
6001 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
6061 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
6121 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
6181 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
6241 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
6301 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
6361 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
6421 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
6481 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
6541 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
6601 tgacgtc
//
