| 质粒名称: | SL-SunTag |
|---|---|
| 目录编号: | 196919 |
| 存储实验室: | Jeffrey Chao |
|
载体骨架基本信息 |
|
| 载体名称: | pSF4 |
| 完整质粒大小: | 11213 bp |
| 载体类型: | 哺乳细胞表达，细菌表达载体 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Renilla luciferase |
| 物种来源: | Renilla reniformis |
| 插入大小: | 930 bp |
|
文献引用方法 |
|
| 材料和方法部分: | SL-SunTag was a gift from Jeffrey Chao (Addgene plasmid # 196919 ; http://n2t.net/addgene:196919 ; RRID:Addgene_196919) |
| 参考文献部分: | Single-molecule imaging reveals translation-dependent destabilization of mRNAs. Dave P, Roth G, Griesbach E, Mateju D, Hochstoeger T, Chao JA. Mol Cell. 2023 Feb 16;83(4):589-606.e6. doi: 10.1016/j.molcel.2023.01.013. Epub 2023 Feb 1. 10.1016/j.molcel.2023.01.013 PubMed 36731471 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS SL-SunTag 11213 bp ds-DNA circular SYN 15-MAR-2023
DEFINITION The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich
stem-loop.
ACCESSION .
VERSION .
KEYWORDS SL-SunTag
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11213)
TITLE Single-molecule imaging reveals translation-dependent
destabilization of mRNAs
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 11213)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 15, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11213
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(12..28)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(12..28)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(25..47)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 36..52
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(60..90)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 105..126
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(243..260)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(414..1002)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(494..513)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1173..2033)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1796..1815
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2034..2138)
/gene="bla"
/label=AmpR promoter
primer_bind 2206..2224
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2262..2284)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2384..2403
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2597..2619
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2611..2628
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2612..2628
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3710..3757)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
protein_bind 3804..3822
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3840..3858
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3876..3894
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3912..3930
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3948..3966
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3984..4002
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 4020..4038
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
intron 4205..4337
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
primer_bind 4320..4339
/label=pMT2-F
/note="Synthetic intron, forward primer"
promoter 4382..4400
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 4404..4413
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4479..4535
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4551..4607
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4623..4679
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4695..4751
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4767..4823
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4839..4895
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4911..4967
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 4983..5039
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5055..5111
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5127..5183
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5199..5255
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5271..5327
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5343..5399
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5415..5471
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5487..5543
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5559..5615
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5631..5687
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5703..5759
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5775..5831
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5847..5903
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5919..5975
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 5991..6047
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 6135..6191
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 6225..7154
/codon_start=1
/product="Renilla luciferase"
/label=hRluc
/note="human codon-optimized"
/translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
CDS 7155..7478
/codon_start=1
/product="destabilization domain that can be stabilized by
Shield1 in the ProteoTuner(TM) system"
/label=DD
/note="L106P mutant of FKBP12"
/translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
LKPE"
polyA_signal 9011..9145
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 9089..9108
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(9339..9355)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(9371..9393)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 9804..10161
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
LTR 10226..10695
/note="long terminal repeat from Moloney murine leukemia
virus"
ORIGIN
1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt tgtcatgaga
1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
2761 cacacacaca atcatacaca taaacacaat cacacacaca cacatgcaca atcacacaca
2821 cacaatcaca cacaaacata catacacaca atcacacaca accatacaca catatacaca
2881 atcacacaca caaacacaat cacacacaca cacatcaatc aatcaatcaa tcaatatacc
2941 aaaaatgata gctcatacct gtaatcttag atgtgggaag gctaaaacag caggctaccc
3001 atgacttcaa ggtcaccctg ggttacagag tagctaatag tctagcttga actatagtgt
3061 aggatcttta aaaaaaaatt aaccaaccaa ccaacaaaca aacaaacaaa caacaacaac
3121 aacaaatgga agacagagga caaaaaaaaa taaaagaaaa agtagaaagc caatggagat
3181 actatcttaa aattagaaat atttatgaca cattctgaat actccatgtt tatttcaagg
3241 aagccaatgt aactttaagt actatggcca tttaatgtaa atacttaaga aaaaaaacca
3301 aattaatttt gatacatgct gcatgtgaaa gacccccgct gacgggtagt caatcactca
3361 gaggagaccc tctcaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
3421 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg
3481 gttgtgggct cgagctcggg gagcagaagc gcgcgaacag aagtcgagct agcttgccaa
3541 acctacaggt ggggtctttc attcccccct ttttctggag actaaataaa atcttttatt
3601 ttatctatgg ctcgtactct ataggcttca gctggtgata ttgttgagtc aaaactagag
3661 cctggaccac tgatatcctg tctttaacaa attggactaa tccggatctg aagttcctat
3721 actttctaga gaataggaac ttcggaatag gaacttcgcg gccagatctt ttgcggccgc
3781 tttgggccgg ccgaattctt tactccctat cagtgataga gaacgtatga agagtttact
3841 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat
3901 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga
3961 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact
4021 ccctatcagt gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc
4081 agagctcgtt tagtgaaccg tcagatgcgc cggtgcagcc ggcgcagcgc ctggagcaat
4141 tccacaacac ttttgtctta taccaacttt ccgtaccact tcctaccctc gtaaaaccgg
4201 tcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc
4261 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
4321 tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac
4381 ttaatacgac tcactatagg ctagccacca tgggcgccat catacacgcg gccgcaatca
4441 tcggtccggg tggatctgga ggtggaggtt ctggaggaga agaacttttg agcaagaatt
4501 atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga gaagaactgc
4561 tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg agcggaagtg
4621 gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga ctaaagaaag
4681 ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac gaagtggctc
4741 ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac cacctcgaaa
4801 atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta tccaagaatt
4861 atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga gaggaactgc
4921 tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga tcgggcagtg
4981 gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga ttaaagaaag
5041 gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat gaggtagcga
5101 gactaaaaaa ggggagtggt tctggcgaag agttgctatc gaaaaattat catcttgaga
5161 acgaagttgc taggctcaaa aagggctcag gctcaggcga ggagttgctc tcgaaaaact
5221 accacttgga aaatgaggtc gcgaggttga aaaaggggag cgggtcgggc gaggagttat
5281 tgagcaaaaa ctatcattta gagaacgaag tcgcgcgctt aaagaaaggc tcgggctcgg
5341 gcgaagaact cttatcgaag aactaccacc tcgaaaatga ggtcgccagg ttgaaaaagg
5401 gcagtggcag cggggaggaa ctcttgagca agaactacca cttggagaat gaggtcgcga
5461 gattgaagaa agggtcgggg agcggcgagg aattgctcag caagaattat catttggaga
5521 acgaagtcgc caggctcaag aaaggctcgg ggtcggggga ggaattgttg agtaaaaact
5581 accacttgga aaatgaagtc gccaggctca aaaaagggag tgggagcggc gaagagttat
5641 tgagcaaaaa ttaccacttg gagaacgaag tggcaaggct caagaaaggg agcggcagcg
5701 gggaggagct cttatcgaag aactaccact tagagaatga agtcgcccgc ttgaagaaag
5761 gctcggggag cggggaagag ctcttgagca agaactacca cttggaaaat gaggtggcgc
5821 gcttgaagaa agggagcggg agcggggaag agttactatc taagaattat catctcgaga
5881 acgaggtggc tcgactaaag aagggctccg gcagtgggga ggaactcctg tcgaagaact
5941 atcatcttga aaatgaggtt gcaagactta aaaaggggtc cggatcaggt gaggaactac
6001 tcagtaagaa ttaccacctg gaaaacgaag tagctcgatt aaagaaaggt tcagggtcgg
6061 gtgaagaact gctctcaaaa gattatcatt tggaaaatga ggttgcacgt ttaaaaaagg
6121 gaagtggcag tggtgaggaa cttctgtcga aaaattatca tctcgagaat gaagtagccc
6181 gacttaaaaa gggttctggc tcgggtcagc ggccgcaccg gtcagcttcc aaggtgtacg
6241 accccgagca acgcaaacgc atgatcactg ggcctcagtg gtgggctcgc tgcaagcaaa
6301 tgaacgtgct ggactccttc atcaactact atgattccga gaagcacgcc gagaacgccg
6361 tgatttttct gcatggtaac gctgcctcca gctacctgtg gaggcacgtc gtgcctcaca
6421 tcgagcccgt ggctagatgc atcatccctg atctgatcgg aatgggtaag tccggcaaga
6481 gcgggaatgg ctcatatcgc ctcctggatc actacaagta cctcaccgct tggttcgagc
6541 tgctgaacct tccaaagaaa atcatctttg tgggccacga ctggggggct tgtctggcct
6601 ttcactactc ctacgagcac caagacaaga tcaaggccat cgtccatgct gagagtgtcg
6661 tggacgtgat cgagtcctgg gacgagtggc ctgacatcga ggaggatatc gccctgatca
6721 agagcgaaga gggcgagaaa atggtgcttg agaataactt cttcgtcgag accatgctcc
6781 caagcaagat catgcggaaa ctggagcctg aggagttcgc tgcctacctg gagccattca
6841 aggagaaggg cgaggttaga cggcctaccc tctcctggcc tcgcgagatc cctctcgtta
6901 agggaggcaa gcccgacgtc gtccagattg tccgcaacta caacgcctac cttcgggcca
6961 gcgacgatct gcctaagatg ttcatcgagt ccgaccctgg gttcttttcc aacgctattg
7021 tcgagggagc taagaagttc cctaacaccg agttcgtgaa ggtgaagggc ctccacttca
7081 gccaggagga cgctccagat gaaatgggta agtacatcaa gagcttcgtg gagcgcgtgc
7141 tgaagaacga gcagatggga gtgcaggtgg aaaccatctc cccaggagac gggcgcacct
7201 tccccaagcg cggccagacc tgtgtggtgc actacaccgg gatgcttgaa gatggaaaga
7261 aagtcgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc aagcaggagg
7321 tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga gccaaactga
7381 ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc ccaccacatg
7441 ccactctcgt cttcgatgtg gagcttctaa aaccggaata agggccaggg aagaactggc
7501 tcggaaagag gaccccacat gttgtagctt caagtttaaa cggatccaac ctacaaacgg
7561 gtggaggatc accccacccg acacttcaca atcaaggggt acaatacaca agggtggagg
7621 aacaccccac cctccagaca cattacacag aaatccaatc aaacagaagc accatcaggg
7681 cttctgctac caaatttatc tcaaaaaact acaacaagga atcaccatca gggattccct
7741 gtgcaatata cgtcaaacga gggccacgac gggaggacga tcacgcctcc cgaatatcgg
7801 catgtctggc tttcgaattc agtgcgtgga gcatcagccc acgcagccaa tcagagtcga
7861 atacaagtcg actttcgcga agagcatcag ccttcgcgcc attcttacac aaaccacact
7921 ctcccctaca ggaacagcat cagcgttcct gcccagtacc caactcaaga aaatttatgt
7981 ccccatgcag catcagcgca tgggccccaa gaatacatcc ccaacaaaat cacatccgag
8041 caccaacagg gctcggagtg ttgtttcttg tccaactgga caaaccctcc atggaccatc
8101 aggccatgga ctctcaccaa caagacaaaa actactcttc tcgaagcagc atcagcgctt
8161 cgaaacactc gagcatacat tgtgcctatt tcttgggtgg acgatcacgc cacccatgct
8221 ctcacgaatt tcaaaacacg gacaaggacg agcaccacca gggctcgtcg ttccacgtcc
8281 aatacgatta cttacctttc gggatcacga tcacggatcc cgcagctaca tcacttccac
8341 tcaggacatt caagcatgca cgatcacggc atgctccaca agtctcaacc acagaaacta
8401 ccaaatgggt tcagcaccag cgaacccact cctacctcaa acctcttccc acaaaactgg
8461 caagcaggat caccgcttgc ccattccaac ataccaaatc aaaaacaatt actggtacag
8521 catcagcgta ccagcccaca tctctcacta ctatcaaaaa ccaaaccgtt cagcaacagc
8581 gaacggtaca cacggaaaaa tcaactggtt tacaaatacg aaagacgatc acgctttcgt
8641 ccagcgcaaa ctattacgaa aaacatccga cgggaagagc aacagccttc ccgcggcgga
8701 aaacctcaca aaaacacgac aaacggatgc acgaacacgg catccgccga caacccacaa
8761 acttacaacc aggcaaacgg tgcaggatca ccgcaccgta catcaaacac ctcagatctc
8821 atttcgcgcg ggatccagac cacctcccct gcgagctaag ctggacagcc aatgacgggt
8881 aagagagtga catttttcac taacctaaga caggagggcc gtcagagcta ctgcctaatc
8941 caaagacggg taaaagtgat aaaaatgtat cactccaacc taagacaggc gcagcttccg
9001 agggatttga gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag
9061 aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac
9121 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
9181 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
9241 tgattatgat ctgaagttcc tatactattt gaagaatagg aacttcggaa taggaacttc
9301 ggatctcata tggccatatg ggggcgccta gagaaggagt gagggctgga taaagggagg
9361 atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga cggggcggat ggaggaagag
9421 gaggcggagg cttagggtgt acaaagggct tgacccaggg aggggggtca aaagccaagg
9481 cttcccaggt cacgatgtag gggacctggt ctgggtgtcc atgcgggcca ggtgaaaaga
9541 ccttgatctt aacctgggtg atgaggtctc ggttaaaggt gccgtctcgc ggccatccga
9601 cgttaaaggt tggccattct gcagagcaga aggtaaccca acgtctcttc ttgacatcta
9661 ccgactggtt gtgagcgatc cgctcgacat ctttccagtg acctaaggtc aaacttaagg
9721 gagtggtaac aggctagccc gggcccatat tttcagacaa atacagaaac acagtcagac
9781 agagacaaca cagaacgatg ctgcagcaga caagacgcgc ggcgcggctt cggtcccaaa
9841 ccgaaagcaa aaattcagac ggaggcggga actgttttag gttctcgtct cctaccagaa
9901 ccacatatcc ctcctctaag gggggtgcac caaagagtcc taaacgatcg ggattttagg
9961 actcaggtcg ggccacaaaa acggcccccg aagtccctgg gacgtctccc agggttgcgg
10021 ccgggtgttc cgaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct
10081 aactagtacc gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac
10141 agataagttg ctggccagct tacctcccgg tggtgggtcg gtggtccctg ggcaggggtc
10201 tcccgatccc ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca
10261 gaggagaccc tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
10321 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgcgc cgagtgaggg
10381 gttgtgggct cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga
10441 actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa
10501 acatctgatg gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc
10561 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag
10621 ctgttccatc tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt
10681 ttggcccata ttcagctgtt ccatctgttc ctgaccttga tctgaacttt tctattctca
10741 gttatgtatt tttccatgcc ttgcaaaatg gcgttactta agctagcttg ccaaacctac
10801 aggtggggtc tttcacatgt atatgtcaaa aataaaaatc aactaattga ctagtaatta
10861 atatgactgg cataatggga aattgatcct gacagatgca aactggcttc tcagcagcgc
10921 atttatgttg tcaactgagg aaggaaacgt taatgacaga aactctaagt aatttccacg
10981 tttatctatt tttatttata ctagctttgg taacaggaat attgcagcat tcatgcacat
11041 tgaaaccctt atgaaataaa aacatctgtg catttaaaat ggaattaaca ttttaaatgt
11101 taaaaaaagc tggcttagct tccccccgcc ccctagggca tagaacaagt caaatgcttt
11161 atatatttga gtttgggatg tattaggaaa ctcctaagag caaagctagc ttg
//
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