| 质粒名称: | pMA2775 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 46884 |
| 存储实验室: | Mikhail Alexeyev |
|
载体骨架基本信息 |
|
| 载体名称: | pMA2780 |
| 载体出品公司: | Homemade |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 6686 bp |
| 完整质粒大小: | 7545 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | exonucleaseIII E. coli |
| 物种来源: | E. coli |
| 插入大小: | 950 bp |
| 基因又名: | exoIII |
| 启动子 | TRE-Tight |
| 标签/融合蛋白: | Myc (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | ctatagtgaatagagttagg |
|
文献引用方法 |
|
| 材料和方法部分: | pMA2775 was a gift from Mikhail Alexeyev (爱迪基因 plasmid # 46884) |
| 参考文献部分: | Persistent damage induces mitochondrial DNA degradation. Shokolenko IN, Wilson GL, Alexeyev MF. DNA Repair (Amst). 2013 Jul;12(7):488-99. doi: 10.1016/j.dnarep.2013.04.023. Epub 2013 May 27. 10.1016/j.dnarep.2013.04.023 PubMed 23721969 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMA2775 7545 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION Lentiviral vector encoding Tet-inducible mitochondrially targeted
myc-tagged E.coli exonuclease III.
ACCESSION .
VERSION .
KEYWORDS pMA2775
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7545)
AUTHORS Shokolenko IN, Wilson GL, Alexeyev MF
TITLE Persistent damage induces mitochondrial DNA degradation.
JOURNAL DNA Repair (Amst). 2013 Jul;12(7):488-99. doi:
10.1016/j.dnarep.2013.04.023. Epub 2013 May 27.
PUBMED 23721969
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pMA2775|
FEATURES Location/Qualifiers
source 1..7545
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(51..70)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 326..629
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 784..804
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 1035..1160
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1657..1890
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2413..2530
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind complement(2629..2645)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2630..2646)
/label=pBluescriptKS
/note="For pBluescript vector"
promoter 2645..2959
/label=tight TRE promoter
/note="Tet-responsive promoter PTight, consisting of seven
tet operator sequences followed by the minimal CMV
promoter"
protein_bind 2653..2671
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2689..2707
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2724..2742
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2760..2778
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2796..2814
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2831..2849
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 2867..2885
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 2932..2956
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 3073..3102
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
primer_bind 3921..3937
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3921..3937
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(3986..4006)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3991..4320
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4171..4306
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4233..4252
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4334..4933
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4334..4353)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4830..4850
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 5551..5731
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(5793..6381)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5873..5892)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6552..7412)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7175..7194
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7413..7517)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgatcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
61 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
121 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
181 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
241 gcgttgacat tgattattga ctagctagtt attaatagta atcaattacg gggtcattag
301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tagaccagat ctgagcctgg
841 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
901 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
961 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
1021 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
1081 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
1141 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
1201 ttcggttaag gccaggggga aagaaaaaat ataaataaac atatagtatg ggcaagcagg
1261 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
1321 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
1381 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
1441 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
1501 ggccgcgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
1561 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
1621 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
1681 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
1741 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
1801 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
1861 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
1921 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
1981 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
2041 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
2101 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
2161 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
2221 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
2281 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
2341 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattcgac aaatggcagt
2401 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
2461 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
2521 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag taccgggccg
2581 gcccgctcta gctagacgta ttaccgccag atcccccatc aagcttatcg ataccgtcga
2641 cctcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
2701 atagagaacg atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact
2761 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
2821 gtcgagttta tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
2881 agagaacgta tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
2941 agtgaaccgt cagatcgcct ggagaattcc gccaccatgc tgtttaatct gaggattctg
3001 ttaaacaatg cagcttttag aaatggtcac aacttcatgg ttcgaaattt tcggtgtgga
3061 caaccactac aagagcagaa actcatcagc gaggaggacc tgggatccaa atttgtctct
3121 tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt cgaaaagcac
3181 caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt tccgctcgaa
3241 gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca ttatggcgtg
3301 gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg tgacgacgaa
3361 gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa tgtcaccgtg
3421 atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt cccggcaaaa
3481 gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg tgataatccg
3541 gtactgatta tgggcgatat gaatatcagc cctacagatc tggacatcgg cattggcgaa
3601 gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga agagcgcgaa
3661 tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc gaatccgcaa
3721 acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga taaccgtggt
3781 ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt agaaaccggc
3841 atcgactatg aaatccgcag catggaaaaa ccgtccgatc acgcccccgt ctgggcgacc
3901 ttccgccgct gagcggccgc tctagaacta gtggatcccc cgggctgcag gaattgatgg
3961 ccgccaccgc ggtggaggac cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
4021 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
4081 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
4141 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
4201 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
4261 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
4321 aagctagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
4381 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
4441 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
4501 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
4561 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
4621 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
4681 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
4741 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
4801 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
4861 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
4921 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
4981 ccatgcatcg atcaattcga tgggggatca attcgattaa gtcgagggtc gatcgacctc
5041 gatcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
5101 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
5161 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
5221 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
5281 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
5341 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
5401 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
5461 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
5521 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
5581 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
5641 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
5701 cccttttagt cagtgtggaa aatctctagc agcatgtgag caaaaggcca gcaaaaggcc
5761 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
5821 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
5881 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
5941 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
6001 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
6061 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
6121 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
6181 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
6241 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
6301 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
6361 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
6421 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
6481 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
6541 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
6601 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
6661 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
6721 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
6781 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
6841 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
6901 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
6961 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
7021 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
7081 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
7141 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
7201 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
7261 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
7321 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
7381 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
7441 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
7501 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
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