出品公司: | |
---|---|
载体名称: | pMEP4 |
质粒类型: | 哺乳动物表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 10440 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | Hygromycin (潮霉素) |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: | |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pMEP4载体质粒基本信息
pMEP4质粒图谱载体图谱和pMEP4载体序列质粒序列多克隆位点信息
pMEP4质粒载体简介
pMEP4质粒序列载体序列
LOCUS pMEP4 10440 bp DNA circular SYN 12-JUL-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10440) AUTHORS Biofeng TITLE Direct Submission JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2 https://www.biofeng.com FEATURES Location/Qualifiers source 1..10440 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal 948..1082 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" polyA_signal 1761..1809 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" CDS complement(1874..2899) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" promoter complement(2939..3084) /label=HSV TK promoter /note="herpes simplex virus thymidine kinase promoter" rep_origin complement(3701..4289) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4460..5320) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5321..5425) /gene="bla" /label=AmpR promoter CDS 5946..7871 /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" rep_origin 8177..9966 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" ORIGIN 1 tctagaggtc gaccaattct catgtttgac agcttatcat cgcagatctg agcttgtggc 61 ttcttctcct tactcttcct cctccttggt gtctgtatgt tagagggccg ttagcatctg 121 catctgctgg ggcctggtcg cattcacctg ctctgccact cactggctgt gtgactctgc 181 agaaattaac ttctctggac ctggcagttt ctcctctcta caatgagaat actggagagt 241 ccttatctta tgggttgcta cagaattaag tgacatctca cacacaacac acttcctaca 301 gtccctgtta cacgctaaaa gtactcaact agcttcggat acgtcatcag caaccacccc 361 acgggttact gtgatgctgc acaattatta agccctggct gctacagagt tgtaacctgt 421 ctgcacttcc aaccggcgcc gcaagcagca ttcccagtcc cgtttcaccc gcgcgctaac 481 ggctcagttc gagtacagga caggagggag gggagctgtg cacacggcgg aggcgcacgg 541 cgtgggcacc cagcacccgg tacactgtgt cctcccgctg cacccagccc cttcagcccg 601 aggcgtcccc gaggcgcaac tgggccgcct tcaggggaac tgaccgcccg cggcccgtgt 661 gcagagccgg gtgcgcccgg cccagtgcgc gcggccgggt gtttcgcttg gagccgcaag 721 tgacttctag cgcggggcgt gtgcagcacg gccggggcgg ggcttttgca ctcgtcccgg 781 ctctttctag ctataaacac tgcttgccgc gctgcactcc accacgcctc ctccaagtcc 841 cagcgaaccc gcgtgcaacc tgtcccgact ctagccgcct cttcagctca cggagggtac 901 cagctgctag caagcttgct agcggccgct cgaggccggc aaggccggat ccagacatga 961 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta 1021 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag 1081 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg gggaggtttt 1141 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg 1201 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 1261 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 1321 gggtgtcggg gcgcagccat gaccggtcga cggcactggg cgccagaaat ccgcgcggtg 1381 gtttttgggg gtcgggggtg tttggcagcc acagacgccc ggtgttcgtg tcgcgccagt 1441 acatgcggtc catgcccagg ccatccaaaa accatgggtc tgtctgctca gtccagtcgt 1501 ggaccagacc ccacgcaacg cccaaaataa taacccccac gaaccataaa ccattcccca 1561 tgggggaccc cgtccctaac ccacggggcc agtggctatg gcagggcctg ccgccccgac 1621 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga 1681 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg 1741 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt 1801 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc 1861 tcccccatct cccctattcc tttgccctcg gacgagtgct ggggcgtcgg tttccactat 1921 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg 1981 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag 2041 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga 2101 gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc 2161 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt 2221 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt 2281 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt 2341 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt 2401 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc 2461 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa 2521 gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt 2581 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca 2641 ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa 2701 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga 2761 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct 2821 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg 2881 tgagttcagg ctttttcata tctcattgcc cgggatctgc ggcacgctgt tgacgctgtt 2941 aagcgggtcg ctgcagggtc gctcggtgtt cgaggccaca cgcgtcacct taatatgcga 3001 agtggacctg ggaccgcgcc gccccgactg catctgcgtg ttcgaattcg ccaatgacaa 3061 gacgctgggc ggggtttgtg tcatcataga actaaagaca tgcaaatata tttcttccgg 3121 ggacaccgcc agcaaacgcg agcaacgggc cacggggatg aagcagggca tggcggccga 3181 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat 3241 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca 3301 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac 3361 ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa 3421 cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg 3481 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga 3541 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc 3601 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcagc 3661 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 3721 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 3781 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 3841 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 3901 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 3961 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 4021 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 4081 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 4141 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 4201 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 4261 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 4321 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 4381 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 4441 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 4501 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 4561 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 4621 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 4681 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 4741 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt 4801 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 4861 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 4921 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 4981 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 5041 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata 5101 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 5161 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 5221 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 5281 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 5341 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 5401 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 5461 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 5521 ttcaagaatt ctcatgtttg acagcttatc atcgataagc tgatcctcac aggccgcacc 5581 cagcttttct tccgttgccc cagtagcatc tctgtctggt gaccttgaag aggaagagga 5641 ggggtcccga gaatccccat ccctaccgtc cagcaaaaag ggggacgagg aatttgaggc 5701 ctggcttgag gctcaggacg caaatcttga ggatgttcag cgggagtttt ccgggctgcg 5761 agtaattggt gatgaggacg aggatggttc ggaggatggg gaattttcag acctggatct 5821 gtctgacagc gaccatgaag gggatgaggg tgggggggct gttggagggg gcaggagtct 5881 gcactccctg tattcactga gcgtcgtcta ataaagatgt ctattgatct cttttagtgt 5941 gaatcatgtc tgacgagggg ccaggtacag gacctggaaa tggcctagga gagaagggag 6001 acacatctgg accagaaggc tccggcggca gtggacctca aagaagaggg ggtgataacc 6061 atggacgagg acggggaaga ggacgaggac gaggaggcgg aagaccagga gccccgggcg 6121 gctcaggatc agggccaaga catagagatg gtgtccggag accccaaaaa cgtccaagtt 6181 gcattggctg caaagggacc cacggtggaa caggagcagg agcaggagcg ggaggggcag 6241 gagcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag 6301 gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg gcaggagggg 6361 caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag 6421 caggaggagg ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag 6481 gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag 6541 gaggaggggc aggagcagga ggggcaggag gggcaggagg ggcaggagca ggaggggcag 6601 gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggggca ggagcaggag 6661 gggcaggagc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag 6721 gaggggcagg agcaggaggg gcaggagggg caggagcagg aggaggggca ggaggggcag 6781 gagcaggagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag 6841 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggagga ggggcaggag 6901 caggaggggc aggagcagga ggtggaggcc ggggtcgagg aggcagtgga ggccggggtc 6961 gaggaggtag tggaggccgg ggtcgaggag gtagtggagg ccgccggggt agaggacgtg 7021 aaagagccag ggggggaagt cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa 7081 agaggcccag gagtcccagt agtcagtcat catcatccgg gtctccaccg cgcaggcccc 7141 ctccaggtag aaggccattt ttccaccctg taggggaagc cgattatttt gaataccacc 7201 aagaaggtgg cccagatggt gagcctgacg tgcccccggg agcgatagag cagggccccg 7261 cagatgaccc aggagaaggc ccaagcactg gaccccgggg tcagggtgat ggaggcaggc 7321 gcaaaaaagg agggtggttt ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg 7381 agaacattgc agaaggttta agagctctcc tggctaggag tcacgtagaa aggactaccg 7441 acgaaggaac ttgggtcgcc ggtgtgttcg tatatggagg tagtaagacc tccctttaca 7501 acctaaggcg aggaactgcc cttgctattc cacaatgtcg tcttacacca ttgagtcgtc 7561 tcccctttgg aatggcccct ggacccggcc cacaacctgg cccgctaagg gagtccattg 7621 tctgttattt catggtcttt ttacaaactc atatatttgc tgaggttttg aaggatgcga 7681 ttaaggacct tgttatgaca aagcccgctc ctacctgcaa tatcagggtg actgtgtgca 7741 gctttgacga tggagtagat ttgcctccct ggtttccacc tatggtggaa ggggctgccg 7801 cggagggtga tgacggagat gacggagatg aaggaggtga tggagatgag ggtgaggaag 7861 ggcaggagtg atgtaacttg ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc 7921 cccgcactaa agaataaatc cccagtagac atcatgcgtg ctgttggtgt atttctggcc 7981 atctgtcttg tcaccatttt cgtcctccca acatggggca attgggcata cccatgttgt 8041 cacgtcactc agctccgcgc tcaacacctt ctcgcgttgg aaaacattag cgacatttac 8101 ctggtgagca atcagacatg cgacggcttt agcctggcct ccttaaattc acctaagaat 8161 gggagcaacc agcatgcagg aaaaggacaa gcagcgaaaa ttcacgcccc cttgggaggt 8221 ggcggcatat gcaaaggata gcactcccac tctactactg ggtatcatat gctgactgta 8281 tatgcatgag gatagcatat gctacccgga tacagattag gatagcatat actacccaga 8341 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga 8401 tataaattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga 8461 tatagattag gatagcctat gctacccaga tatagattag gatagcatat gctacccaga 8521 tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta cccagatata 8581 aattaggata gcatatacta ccctaatctc tattaggata gcatatgcta cccggataca 8641 gattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata 8701 gattaggata gcctatgcta cccagatata aattaggata gcatatacta cccagatata 8761 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata 8821 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca tggcaacatt 8881 agcccaccgt gctctcagcg acctcgtgaa tatgaggacc aacaaccctg tgcttggcgc 8941 tcaggcgcaa gtgtgtgtaa tttgtcctcc agatcgcagc aatcgcgccc ctatcttggc 9001 ccgcccacct acttatgcag gtattccccg gggtgccatt agtggttttg tgggcaagtg 9061 gtttgaccgc agtggttagc ggggttacaa tcagccaagt tattacaccc ttattttaca 9121 gtccaaaacc gcagggcggc gtgtgggggc tgacgcgtgc ccccactcca caatttcaaa 9181 aaaaagagtg gccacttgtc tttgtttatg ggccccattg gcgtggagcc ccgtttaatt 9241 ttcgggggtg ttagagacaa ccagtggagt ccgctgctgt cggcgtccac tctctttccc 9301 cttgttacaa atagagtgta acaacatggt tcacctgtct tggtccctgc ctgggacaca 9361 tcttaataac cccagtatca tattgcacta ggattatgtg ttgcccatag ccataaattc 9421 gtgtgagatg gacatccagt ctttacggct tgtccccacc ccatggattt ctattgttaa 9481 agatattcag aatgtttcat tcctacacta gtatttattg cccaaggggt ttgtgagggt 9541 tatattggtg tcatagcaca atgccaccac tgaacccccc gtccaaattt tattctgggg 9601 gcgtcacctg aaaccttgtt ttcgagcacc tcacatacac cttactgttc acaactcagc 9661 agttattcta ttagctaaac gaaggagaat gaagaagcag gcgaagattc aggagagttc 9721 actgcccgct ccttgatctt cagccactgc ccttgtgact aaaatggttc actaccctcg 9781 tggaatcctg accccatgta aataaaaccg tgacagctca tggggtggga gatatcgctg 9841 ttccttagga cccttttact aaccctaatt cgatagcata tgcttcccgt tgggtaacat 9901 atgctattga attagggtta gtctggatag tatatactac tacccgggaa gcatatgcta 9961 cccgtttagg gttaacaagg gggccttata aacactattg ctaatgccct cttgagggtc 10021 cgcttatcgg tagctacaca ggcccctctg attgacgttg gtgtagcctc ccgtagtctt 10081 cctgggcccc tgggaggtac atgtccccca gcattggtgt aagagcttca gccaagagtt 10141 acacataaag gcaatgttgt gttgcagtcc acagactgca aagtctgctc caggatgaaa 10201 gccactcagt gttggcaaat gtgcacatcc atttataagg atgtcaacta cagtcagaga 10261 acccctttgt gtttggtccc cccccgtgtc acatgtggaa cagggcccag ttggcaagtt 10321 gtaccaacca actgaaggga ttacatgcac tgccccgaat acaaaacaaa agcgctcctc 10381 gtaccagcga agaaggggca gagatgccgt agtcaggttt agttcgtccg gcggcggggc //