| 出品公司: | |
|---|---|
| 载体名称: | pMEP4 |
| 质粒类型: | 哺乳动物表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 10440 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Hygromycin (潮霉素) |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMEP4 10440 bp DNA circular SYN 12-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10440)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 12, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10440
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal 948..1082
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 1761..1809
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
CDS complement(1874..2899)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
promoter complement(2939..3084)
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
rep_origin complement(3701..4289)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4460..5320)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5321..5425)
/gene="bla"
/label=AmpR promoter
CDS 5946..7871
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
rep_origin 8177..9966
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
ORIGIN
1 tctagaggtc gaccaattct catgtttgac agcttatcat cgcagatctg agcttgtggc
61 ttcttctcct tactcttcct cctccttggt gtctgtatgt tagagggccg ttagcatctg
121 catctgctgg ggcctggtcg cattcacctg ctctgccact cactggctgt gtgactctgc
181 agaaattaac ttctctggac ctggcagttt ctcctctcta caatgagaat actggagagt
241 ccttatctta tgggttgcta cagaattaag tgacatctca cacacaacac acttcctaca
301 gtccctgtta cacgctaaaa gtactcaact agcttcggat acgtcatcag caaccacccc
361 acgggttact gtgatgctgc acaattatta agccctggct gctacagagt tgtaacctgt
421 ctgcacttcc aaccggcgcc gcaagcagca ttcccagtcc cgtttcaccc gcgcgctaac
481 ggctcagttc gagtacagga caggagggag gggagctgtg cacacggcgg aggcgcacgg
541 cgtgggcacc cagcacccgg tacactgtgt cctcccgctg cacccagccc cttcagcccg
601 aggcgtcccc gaggcgcaac tgggccgcct tcaggggaac tgaccgcccg cggcccgtgt
661 gcagagccgg gtgcgcccgg cccagtgcgc gcggccgggt gtttcgcttg gagccgcaag
721 tgacttctag cgcggggcgt gtgcagcacg gccggggcgg ggcttttgca ctcgtcccgg
781 ctctttctag ctataaacac tgcttgccgc gctgcactcc accacgcctc ctccaagtcc
841 cagcgaaccc gcgtgcaacc tgtcccgact ctagccgcct cttcagctca cggagggtac
901 cagctgctag caagcttgct agcggccgct cgaggccggc aaggccggat ccagacatga
961 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
1021 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
1081 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg gggaggtttt
1141 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg
1201 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
1261 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
1321 gggtgtcggg gcgcagccat gaccggtcga cggcactggg cgccagaaat ccgcgcggtg
1381 gtttttgggg gtcgggggtg tttggcagcc acagacgccc ggtgttcgtg tcgcgccagt
1441 acatgcggtc catgcccagg ccatccaaaa accatgggtc tgtctgctca gtccagtcgt
1501 ggaccagacc ccacgcaacg cccaaaataa taacccccac gaaccataaa ccattcccca
1561 tgggggaccc cgtccctaac ccacggggcc agtggctatg gcagggcctg ccgccccgac
1621 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga
1681 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg
1741 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
1801 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
1861 tcccccatct cccctattcc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
1921 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
1981 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
2041 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
2101 gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
2161 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
2221 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
2281 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
2341 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
2401 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
2461 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
2521 gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt
2581 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca
2641 ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
2701 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
2761 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
2821 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
2881 tgagttcagg ctttttcata tctcattgcc cgggatctgc ggcacgctgt tgacgctgtt
2941 aagcgggtcg ctgcagggtc gctcggtgtt cgaggccaca cgcgtcacct taatatgcga
3001 agtggacctg ggaccgcgcc gccccgactg catctgcgtg ttcgaattcg ccaatgacaa
3061 gacgctgggc ggggtttgtg tcatcataga actaaagaca tgcaaatata tttcttccgg
3121 ggacaccgcc agcaaacgcg agcaacgggc cacggggatg aagcagggca tggcggccga
3181 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
3241 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
3301 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac
3361 ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa
3421 cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg
3481 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga
3541 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc
3601 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcagc
3661 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
3721 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
3781 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
3841 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
3901 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
3961 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
4021 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
4081 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
4141 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
4201 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
4261 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
4321 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
4381 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
4441 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
4501 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
4561 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
4621 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
4681 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
4741 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
4801 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
4861 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
4921 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
4981 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
5041 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
5101 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
5161 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
5221 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
5281 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
5341 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
5401 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
5461 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
5521 ttcaagaatt ctcatgtttg acagcttatc atcgataagc tgatcctcac aggccgcacc
5581 cagcttttct tccgttgccc cagtagcatc tctgtctggt gaccttgaag aggaagagga
5641 ggggtcccga gaatccccat ccctaccgtc cagcaaaaag ggggacgagg aatttgaggc
5701 ctggcttgag gctcaggacg caaatcttga ggatgttcag cgggagtttt ccgggctgcg
5761 agtaattggt gatgaggacg aggatggttc ggaggatggg gaattttcag acctggatct
5821 gtctgacagc gaccatgaag gggatgaggg tgggggggct gttggagggg gcaggagtct
5881 gcactccctg tattcactga gcgtcgtcta ataaagatgt ctattgatct cttttagtgt
5941 gaatcatgtc tgacgagggg ccaggtacag gacctggaaa tggcctagga gagaagggag
6001 acacatctgg accagaaggc tccggcggca gtggacctca aagaagaggg ggtgataacc
6061 atggacgagg acggggaaga ggacgaggac gaggaggcgg aagaccagga gccccgggcg
6121 gctcaggatc agggccaaga catagagatg gtgtccggag accccaaaaa cgtccaagtt
6181 gcattggctg caaagggacc cacggtggaa caggagcagg agcaggagcg ggaggggcag
6241 gagcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag
6301 gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg gcaggagggg
6361 caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag
6421 caggaggagg ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag
6481 gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag
6541 gaggaggggc aggagcagga ggggcaggag gggcaggagg ggcaggagca ggaggggcag
6601 gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggggca ggagcaggag
6661 gggcaggagc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag
6721 gaggggcagg agcaggaggg gcaggagggg caggagcagg aggaggggca ggaggggcag
6781 gagcaggagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag
6841 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggagga ggggcaggag
6901 caggaggggc aggagcagga ggtggaggcc ggggtcgagg aggcagtgga ggccggggtc
6961 gaggaggtag tggaggccgg ggtcgaggag gtagtggagg ccgccggggt agaggacgtg
7021 aaagagccag ggggggaagt cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa
7081 agaggcccag gagtcccagt agtcagtcat catcatccgg gtctccaccg cgcaggcccc
7141 ctccaggtag aaggccattt ttccaccctg taggggaagc cgattatttt gaataccacc
7201 aagaaggtgg cccagatggt gagcctgacg tgcccccggg agcgatagag cagggccccg
7261 cagatgaccc aggagaaggc ccaagcactg gaccccgggg tcagggtgat ggaggcaggc
7321 gcaaaaaagg agggtggttt ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg
7381 agaacattgc agaaggttta agagctctcc tggctaggag tcacgtagaa aggactaccg
7441 acgaaggaac ttgggtcgcc ggtgtgttcg tatatggagg tagtaagacc tccctttaca
7501 acctaaggcg aggaactgcc cttgctattc cacaatgtcg tcttacacca ttgagtcgtc
7561 tcccctttgg aatggcccct ggacccggcc cacaacctgg cccgctaagg gagtccattg
7621 tctgttattt catggtcttt ttacaaactc atatatttgc tgaggttttg aaggatgcga
7681 ttaaggacct tgttatgaca aagcccgctc ctacctgcaa tatcagggtg actgtgtgca
7741 gctttgacga tggagtagat ttgcctccct ggtttccacc tatggtggaa ggggctgccg
7801 cggagggtga tgacggagat gacggagatg aaggaggtga tggagatgag ggtgaggaag
7861 ggcaggagtg atgtaacttg ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc
7921 cccgcactaa agaataaatc cccagtagac atcatgcgtg ctgttggtgt atttctggcc
7981 atctgtcttg tcaccatttt cgtcctccca acatggggca attgggcata cccatgttgt
8041 cacgtcactc agctccgcgc tcaacacctt ctcgcgttgg aaaacattag cgacatttac
8101 ctggtgagca atcagacatg cgacggcttt agcctggcct ccttaaattc acctaagaat
8161 gggagcaacc agcatgcagg aaaaggacaa gcagcgaaaa ttcacgcccc cttgggaggt
8221 ggcggcatat gcaaaggata gcactcccac tctactactg ggtatcatat gctgactgta
8281 tatgcatgag gatagcatat gctacccgga tacagattag gatagcatat actacccaga
8341 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
8401 tataaattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
8461 tatagattag gatagcctat gctacccaga tatagattag gatagcatat gctacccaga
8521 tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta cccagatata
8581 aattaggata gcatatacta ccctaatctc tattaggata gcatatgcta cccggataca
8641 gattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
8701 gattaggata gcctatgcta cccagatata aattaggata gcatatacta cccagatata
8761 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
8821 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca tggcaacatt
8881 agcccaccgt gctctcagcg acctcgtgaa tatgaggacc aacaaccctg tgcttggcgc
8941 tcaggcgcaa gtgtgtgtaa tttgtcctcc agatcgcagc aatcgcgccc ctatcttggc
9001 ccgcccacct acttatgcag gtattccccg gggtgccatt agtggttttg tgggcaagtg
9061 gtttgaccgc agtggttagc ggggttacaa tcagccaagt tattacaccc ttattttaca
9121 gtccaaaacc gcagggcggc gtgtgggggc tgacgcgtgc ccccactcca caatttcaaa
9181 aaaaagagtg gccacttgtc tttgtttatg ggccccattg gcgtggagcc ccgtttaatt
9241 ttcgggggtg ttagagacaa ccagtggagt ccgctgctgt cggcgtccac tctctttccc
9301 cttgttacaa atagagtgta acaacatggt tcacctgtct tggtccctgc ctgggacaca
9361 tcttaataac cccagtatca tattgcacta ggattatgtg ttgcccatag ccataaattc
9421 gtgtgagatg gacatccagt ctttacggct tgtccccacc ccatggattt ctattgttaa
9481 agatattcag aatgtttcat tcctacacta gtatttattg cccaaggggt ttgtgagggt
9541 tatattggtg tcatagcaca atgccaccac tgaacccccc gtccaaattt tattctgggg
9601 gcgtcacctg aaaccttgtt ttcgagcacc tcacatacac cttactgttc acaactcagc
9661 agttattcta ttagctaaac gaaggagaat gaagaagcag gcgaagattc aggagagttc
9721 actgcccgct ccttgatctt cagccactgc ccttgtgact aaaatggttc actaccctcg
9781 tggaatcctg accccatgta aataaaaccg tgacagctca tggggtggga gatatcgctg
9841 ttccttagga cccttttact aaccctaatt cgatagcata tgcttcccgt tgggtaacat
9901 atgctattga attagggtta gtctggatag tatatactac tacccgggaa gcatatgcta
9961 cccgtttagg gttaacaagg gggccttata aacactattg ctaatgccct cttgagggtc
10021 cgcttatcgg tagctacaca ggcccctctg attgacgttg gtgtagcctc ccgtagtctt
10081 cctgggcccc tgggaggtac atgtccccca gcattggtgt aagagcttca gccaagagtt
10141 acacataaag gcaatgttgt gttgcagtcc acagactgca aagtctgctc caggatgaaa
10201 gccactcagt gttggcaaat gtgcacatcc atttataagg atgtcaacta cagtcagaga
10261 acccctttgt gtttggtccc cccccgtgtc acatgtggaa cagggcccag ttggcaagtt
10321 gtaccaacca actgaaggga ttacatgcac tgccccgaat acaaaacaaa agcgctcctc
10381 gtaccagcga agaaggggca gagatgccgt agtcaggttt agttcgtccg gcggcggggc
//
