采用 Gateway® 技术的 ProQuest™ 双杂交系统 (ProQuest™ Two-Hybrid System with Gateway® Technology) 是一种基于酵母的体内系统,用于鉴定两种蛋白之间的相互作用。 这种相互作用会重构功能性转录因子,该转录因子激活整合在染色体上的报告基因,而转录激活与否可通过选择性培养基上的细胞生长情况来进行监测。
ProQuest™ 系统 (ProQuest™ System) 具有如下特点:
• 采用 Gateway® 技术,能够快速简便地生成“诱饵”和“猎物”构建体,有利于下游应用
• 低拷贝 DBD(DNA 结合结构域)和 AD(活化结构域)载体 (ARS/CEN),可控制过量表达,提高重复性
• 三种不同报告基因(HIS3、URA3 和 lacZ),具有独立的启动子区域,可快速筛除假阳性。 URA3 报告基因可用于阳性选择和阴性选择,从而可以使用高级双杂交技术,例如反向双杂交。
• 一组由强到弱、基于 Gateway® 的相互作用对照组,用于评估实验结果以及实验组相互作用的强度。
LOCUS pDEST32 12288 bp DNA circular SYN 2-SEP-2003
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTDATE|38836800|
COMMENT VNTDBDATE|312633458|
COMMENT LSOWNER|
COMMENT VNTNAME|pDEST32|
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..12288
/invitrogen="666"
/vntifkey="98"
misc_recomb 6792..6916
/invitrogen="2690000"
/vntifkey="86"
/label=attR1
misc_recomb complement(8513..8637)
/invitrogen="2700000"
/vntifkey="86"
/label=attR2
CDS 8167..8472
/invitrogen="1060000"
/vntifkey="4"
/label=ccdB
CDS 7166..7825
/invitrogen="1080000"
/vntifkey="4"
/label=Cm(R)
rep_origin 9359..9814
/invitrogen="2520000"
/vntifkey="33"
/label=f1\origin
CDS 6336..6791
/invitrogen="1120000"
/vntifkey="4"
/label=Gal4\DBD
CDS 1450..1983
/invitrogen="1150000"
/vntifkey="4"
/label=Gm(R)
CDS 10520..11626
/invitrogen="1250000"
/vntifkey="4"
/label=LEU2
terminator complement(3399..3647)
/invitrogen="2560000"
/vntifkey="43"
/label=CYC1\transcription\terminator
promoter 1234..1261
/invitrogen="2250000"
/vntifkey="29"
/label=Pc\promoter
rep_origin 2313..2986
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
terminator 8837..9168
/invitrogen="2565000"
/vntifkey="43"
/label=ADH1\transcription\terminator
promoter 4857..6308
/invitrogen="2221000"
/vntifkey="29"
/label=ADH1\promoter
CDS complement(3940..4389)
/invitrogen="1335000"
/vntifkey="4"
/label=CYH2
promoter complement(4390..4705)
/invitrogen="2222000"
/vntifkey="29"
/label=CYH2\promoter
3'UTR complement(3755..3939)
/invitrogen="123000098"
/vntifkey="50"
/label=CYH2\3'\UTR
centromere 68..443
/invitrogen="123000099"
/vntifkey="5"
/label=ARS4/Cen6
BASE COUNT 3472 a 2635 c 2722 g 3458 t 1 others
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
661 ctcatgagac aataaccctg ataaatgctt caataatctg cagtgcgcag ggcccgtgtc
721 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
781 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
841 ttgctggagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
901 tcggtagcca accactagaa ctatagctag agtcctgggc gaacaaacga tgctcgcctt
961 ccagaaaacc gaggatgcga accacttcat ccggggtcag caccaccggc aagcgccgcg
1021 acggccgagg tcttccgatc tcctgaagcc agggcagatc cgtgcacagc accttgccgt
1081 agaagaacag caaggccgcc aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt
1141 tcgccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca
1201 caccgtggaa acggatgaag gcacgaaccc agttgacata agcctgttcg gttcgtaaac
1261 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
1321 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta
1381 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
1441 gcagcaacga tgttacgcag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
1501 aagttaggtg gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc
1561 aaatccatgc gggctgctct tgatcttttc ggtcgtgagt tcggagacgt agccacctac
1621 tcccaacatc agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc
1681 gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc
1741 aggtttgagc agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac
1801 cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt
1861 ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat
1921 acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc
1981 taacaattcg ttcaagccga gatcggcttc ccggcctaat aggttgtatt gatgttggac
2041 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
2101 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
2161 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
2221 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgncatgacc aaaatccctt
2281 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
2341 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
2401 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
2461 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
2521 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
2581 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
2641 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
2701 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
2761 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
2821 ttccaggggg gaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
2881 agcgtcgatt tttgtgatgc tcgtcagggg ggccgagcct atggaaaaac gccagcaacg
2941 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
3001 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
3061 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
3121 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
3181 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttacctc actcattagg
3241 caccccaggc tttacacttt atgcttccgg ctcctatgtt gtgtggaatt gtgagcggat
3301 aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgga attaaccctc
3361 actaaaggga acaaaagctg gtaccgatcc cgagctttgc aaattaaagc cttcgagcgt
3421 cccaaaacct tctcaagcaa ggttttcagt ataatgttac atgcgtacac gcgtctgtac
3481 agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat aactataaaa
3541 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat
3601 gtggggggag ggcgtgaatg taagcgtgac ataactaatt acatgatatc gacaaaggaa
3661 aaggggcctg tttactcaca ggcttttttc aagtaggtaa ttaagtcgtt tctgtctttt
3721 tccttcttca acccaccaaa ggccatcttg gtactttttt tttttttttt tttttttttt
3781 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
3841 ttttttttca tagaaataat acagaagtag atgttgaatt agattaaact gaagatatat
3901 aatttattgg aaaatacata gagctttttg ttgatgcgct taagcgatca attcaacaac
3961 accaccagca gctctgattt tttcttcagc caacttggag acgaatctag ctttgacgat
4021 aactggaaca tttggaattc tacccttacc caagatctta ccgtaaccgg ctgccaaagt
4081 gtcaataact ggagcagttt ccttagaagc agatttcaag tattggtctc tcttgtcttc
4141 tgggatcaat gtccacaatt tgtccaagtt caagactggc ttccagaaat gagcttgttg
4201 cttgtggaag tatctcatac caaccttacc gaaataacct ggatggtatt tatccatgtt
4261 aattctgtgg tgatgttgac caccggccat acctctacca ccggggtgct ttctgtgctt
4321 accgatacga cctttaccgg ctgagacgtg acctctgtgc tttctagtct tagtgaatct
4381 ggaaggcatt cttgattagt tggatgattg ttctgggatt taatgcaaaa atcacttaag
4441 aaggaaaatc aacggagaaa gcaaacgcca tcttaaatat acgggataca gatgaaaggg
4501 tttgaaccta tctggaaaat agcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc
4561 gtctctgcgg gctattcacg cgccagagga aaataggaaa aataacaggg cattagaaaa
4621 ataattttga ttttggtaat gtgtgggtcc tggtgtacag atgttacatt ggttacagta
4681 ctcttgtttt tgctgtgttt ttcgatgaat ctccaaaatg gttgttagca catggaagag
4741 tcaccgatgc taagttatct ctatgtaagc tacgtggcgt gacttttgat gaagccgcac
4801 aagagataca ggattggcaa ctgcaaatag aatctgggga tcccccctcg agatccggga
4861 tcgaagaaat gatggtaaat gaaataggaa atcaaggagc atgaaggcaa aagacaaata
4921 taagggtcga acgaaaaata aagtgaaaag tgttgatatg atgtatttgg ctttgcggcg
4981 ccgaaaaaac gagtttacgc aattgcacaa tcatgctgac tctgtggcgg acccgcgctc
5041 ttgccggccc ggcgataacg ctgggcgtga ggctgtgccc ggcggagttt tttgcgcctg
5101 cattttccaa ggtttaccct gcgctaaggg gcgagattgg agaagcaata agaatgccgg
5161 ttggggttgc gatgatgacg accacgacaa ctggtgtcat tatttaagtt gccgaaagaa
5221 cctgagtgca tttgcaacat gagtatacta gaagaatgag ccaagacttg cgagacgcga
5281 gtttgccggt ggtgcgaaca atagagcgac catgaccttg aaggtgagac gcgcataacc
5341 gctagagtac tttgaagagg aaacagcaat agggttgcta ccagtataaa tagacaggta
5401 catacaacac tggaaatggt tgtctgtttg agtacgcttt caattcattt gggtgtgcac
5461 tttattatgt tacaatatgg aagggaactt tacacttctc ctatgcacat atattaatta
5521 aagtccaatg ctagtagaga aggggggtaa cacccctccg cgctcttttc cgattttttt
5581 ctaaaccgtg gaatatttcg gatatccttt tgttgtttcc gggtgtacaa tatggacttc
5641 ctcttttctg gcaaccaaac ccatacatcg ggattcctat aataccttcg ttggtctccc
5701 taacatgtag gtggcggagg ggagatatac aatagaacag ataccagaca agacataatg
5761 ggctaaacaa gactacacca attacactgc ctcattgatg gtggtacata acgaactaat
5821 actgtagccc tagacttgat agccatcatc atatcgaagt ttcactaccc tttttccatt
5881 tgccatctat tgaagtaata ataggcgcat gcaacttctt ttcttttttt ttcttttctc
5941 tctcccccgt tgttgtctca ccatatccgc aatgacaaaa aaaatgatgg aagacactaa
6001 aggaaaaaat taacgacaaa gacagcacca acagatgtcg ttgttccaga gctgatgagg
6061 ggtatcttcg aacacacgaa actttttcct tccttcattc acgcacacta ctctctaatg
6121 agcaacggta tacggccttc cttccagtta cttgaatttg aaataaaaaa agtttgccgc
6181 tttgctatca agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc
6241 tcgttccctt tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac
6301 aatcaactcc aagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac
6361 aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg
6421 ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc
6481 tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc
6541 tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata
6601 taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag
6661 atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg
6721 cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgtcga
6781 ggtcgaatca aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
6841 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
6901 atatccagtc actatggcgg ccgctaagtt ggcagcatca cccgacgcac tttgcgccga
6961 ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga
7021 taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg
7081 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
7141 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
7201 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
7261 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
7321 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
7381 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
7441 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
7501 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
7561 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
7621 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
7681 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
7741 tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
7801 cgatgagtgg cagggcgggg cgtaatctag aggatccggc ttactaaaag ccagataaca
7861 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg
7921 aagtatgtca aaaagaggtg tgctatgaag cagcgtatta cagtgacagt tgacagcgac
7981 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca
8041 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga
8101 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacagggact
8161 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
8221 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt
8281 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
8341 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa
8401 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
8461 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga
8521 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
8581 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtttg
8641 atggccgcta agtaagtaag acgtcgagct ctaagtaagt aacggccgcc accgcggtgg
8701 agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg tcggcttgtc
8761 taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta gatacgttgt
8821 tgacacttct aaataagcga atttcttatg atttatgatt tttattatta aataagttat
8881 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
8941 gttcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc
9001 tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccattt
9061 cacccaattg tagatatgct aactccagca atgagttgat gaatctcggt gtgtatttta
9121 tgtcctcaga ggacaatacc tgttgtaatc gttcttccac acggatccca attcgcccta
9181 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
9241 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
9301 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac
9361 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
9421 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
9481 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
9541 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
9601 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
9661 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
9721 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
9781 gcgaatttta acaaaatatt aacgtttaca atttcctgat gcggtatttt ctccttacgc
9841 atctgtgcgg tatttcacac cgcatatcga ccggtcgagg agaacttcta gtatatccac
9901 atacctaata ttattgcctt attaaaaatg gaatcggaac aattacatca aaatccacat
9961 tctcttcaaa atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt
10021 tataggataa ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa
10081 ggcgcctgat tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta
10141 agatgcaaga gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca
10201 cacaggggcg ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag
10261 aggtcgcctg acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag
10321 aggccggaac cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca
10381 caatacttga agttgacaat attatttaag gacctattgt tttttccaat aggtggttag
10441 caatcgtctt actttctaac ttttcttacc ttttacattt cagcaatata tatatatatt
10501 tcaaggatat accattctaa tgtctgcccc tatgtctgcc cctaagaaga tcgtcgtttt
10561 gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat
10621 ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc
10681 tatcgatgct acaggtgtcc cacttccaga tgaggcgctg gaagcctcca agaaggttga
10741 tgccgttttg ttaggtgctg tgggtggtcc taaatggggt accggtagtg ttagacctga
10801 acaaggttta ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa
10861 ctttgcatcc gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac
10921 tgacttcgtt gttgtcagag aattagtggg aggtatttac tttggtaaga gaaaggaaga
10981 cgatggtgat ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat
11041 cacaagaatg gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt
11101 ggataaagct aatgttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat
11161 caagaacgaa ttccctacat tgaaggttca acatcaattg attgattctg ccgccatgat
11221 cctagttaag aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga
11281 tatcatctcc gatgaagcct ccgttatccc aggttccttg ggtttgttgc catctgcgtc
11341 cttggcctct ttgccagaca agaacaccgc atttggtttg tacgaaccat gccacggttc
11401 tgctccagat ttgccaaaga ataaggttga ccctatcgcc actatcttgt ctgctgcaat
11461 gatgttgaaa ttgtcattga acttgcctga agaaggtaag gccattgaag atgcagttaa
11521 aaaggttttg gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga
11581 agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt
11641 tttatgatat ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt
11701 acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac atacatttat
11761 caagaaggag aaaaaggagg atagtaaagg aatacaggta agcaaattga tactaatggc
11821 tcaacgtgat aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac
11881 cacacaaaaa gttaggtgta acagaaaatc atgaaactac gattcctaat ttgatattgg
11941 aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg acctgaccat
12001 ttgatggagt ttaagtcaat accttcttga accatttccc ataatggtga aagttccctc
12061 aagaatttta ctctgtcaga aacggcctta cgacgtagtc gatatggtgc actctcagta
12121 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
12181 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
12241 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//