| 出品公司: | Gyuris J, Golemis E, Chertkov H, Brent R. |
|---|---|
| 载体名称: | pJG4-5 |
| 质粒类型: | 酵母质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 6449 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pJG4-5载体 pJG45质粒基本信息
pJG4-5质粒图谱 pJG45载体图谱和pJG4-5载体序列 pJG45质粒序列多克隆位点信息
pJG4-5质粒 pJG45载体简介
pJG4-5质粒序列 pJG45载体序列
LOCUS pJG4-5 6449 bp ds-DNA circular SYN 23-FEB-2014
DEFINITION Yeast two-hybrid vector with a GAL1 promoter, for fusing the B42
transcriptional activator to a partner protein. Also known as
pB42AD.
ACCESSION U89961.1
VERSION .
KEYWORDS pJG4-5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6449)
AUTHORS Gyuris J, Golemis E, Chertkov H, Brent R.
TITLE Cdi1, a human G1 and S phase protein phosphatase that associates
with Cdk2.
JOURNAL Cell 1993;75:791-803.
PUBMED 8242750
REFERENCE 2 (bases 1 to 6449)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Friday, February 14, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6449
/organism="synthetic DNA construct"
/lab_host="Saccharomyces cerevisiae"
/mol_type="other DNA"
promoter 81..522
/gene="S. cerevisiae GAL1"
/note="GAL1 promoter"
/note="inducible promoter, regulated by Gal4"
protein_bind 81..198
/bound_moiety="Gal4"
/note="UAS"
/note="upstream activating sequence mediating
Gal4-dependent induction"
CDS 534..536
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 546..566
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
CDS 573..809
/codon_start=1
/product="yeast transcriptional activator cretaed from E.
coli genomic DNA fragments (Ma and Ptashne, 1987)"
/note="B42 transcriptional activator"
/translation="INKDIEECNAIIEQFIDYLRTGQEMPMEMADQAINVVPGMTPKTI
LHAGPPIQPDWLKSNGFHEIEADVNDTSLLLSGD"
CDS 816..842
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
misc_feature 849..866
/note="MCS"
/note="multiple cloning site"
terminator 1004..1191
/gene="S. cerevisiae ADH1"
/note="ADH1 terminator"
/note="transcription terminator for alcohol dehydrogenase
1"
rep_origin 1713..3055
/note="2u ori"
/note="yeast 2u plasmid origin of replication"
CDS complement(3416..4090)
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/note="TRP1"
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
promoter complement(4091..4192)
/gene="S. cerevisiae TRP1"
/note="TRP1 promoter"
promoter 4295..4399
/gene="bla"
/note="AmpR promoter"
CDS 4403..5263
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="SIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIE
LDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSP
VTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWE
PELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSAL
PAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASL
IKHW*L"
rep_origin 5431..6019
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg
61 ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg
121 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc
181 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag
241 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa
301 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa
361 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac
421 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa
481 caaaaaattg ttaatatacc tctatacttt aacgtcaagg aggaattaag cttatgggtg
541 ctcctccaaa aaagaagaga aaggtagctg gtatcaataa agatatcgag gagtgcaatg
601 ccatcattga gcagtttatc gactacctgc gcaccggaca ggagatgccg atggaaatgg
661 cggatcaggc gattaacgtg gtgccgggca tgacgccgaa aaccattctt cacgccgggc
721 cgccgatcca gcctgactgg ctgaaatcga atggttttca tgaaattgaa gcggatgtta
781 acgataccag cctcttgctg agtggagatg cctcctaccc ttatgatgtg ccagattatg
841 cctctcccga attcggccga ctcgagaagc tttggacttc ttcgccagag gtttggtcaa
901 gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa
961 gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt
1021 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
1081 ctcttaggtt ttaaaacgaa aattcttgtt cttgagtaac tctttcctgt aggtcaggtt
1141 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccgag
1201 caaatgcctg caaatcgctc cccatttcac ccaattgtag atatgctaac tccagcaatg
1261 agttgatgaa tctcggtgtg tattttatgt cctcagagga caacacctgt tgtaatcgtt
1321 cttccacacg gatcctctag agtcgactag cggccgcttc gacctgcagc aattctgaac
1381 cagtcctaaa acgagtaaat aggaccggca attcttcaag caataaacag gaataccaat
1441 tattaaaaga taacttagtc agatcgtaca ataaagcttt gaagaaaaat gcgccttatt
1501 caatctttgc tataaaaaat ggcccaaaat ctcacattgg aagacatttg atgacctcat
1561 ttctttcaat gaagggccta acggagttga ctaatgttgt gggaaattgg agcgataagc
1621 gtgcttctgc cgtggccagg acaacgtata ctcatcagat aacagcaata cctgatcact
1681 acttcgcact agtttctcgg tactatgcat atgatccaat atcaaaggaa atgatagcat
1741 tgaaggatga gactaatcca attgaggagt ggcagcatat agaacagcta aagggtagtg
1801 ctgaaggaag catacgatac cccgcatgga atgggataat atcacaggag gtactagact
1861 acctttcatc ctacataaat agacgcatat aagtacgcat ttaagcataa acacgcacta
1921 tgccgttctt ctcatgtata tatatataca ggcaacacgc agatataggt gcgacgtgaa
1981 cagtgagctg tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg
2041 ctttgaagtt cctattccga agttcctatt ctctagaaag tataggaact tcagagcgct
2101 tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg caccggactg
2161 taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa aagtatctct
2221 ttgctatata tctctgtgct atatccctat ataacctacc catccacctt tcgctccttg
2281 aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat tactctttag
2341 acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac gaaaatgtaa
2401 acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata attttctgac
2461 caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa tccacatcgg
2521 tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc ttcgctagta
2581 atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat agacaccaaa
2641 gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga gactgcatta
2701 tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa atagcgctct
2761 cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta aaatagcgct
2821 ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
2881 gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt tggtaaaata
2941 gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa
3001 ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct tcgttaacaa
3061 agatatgcta ttgaagtgca agatggaaac gcagaaaatg aaccggggat gcgacgtgca
3121 agattaccta tgcaatagat gcaatagttt ctccaggaac cgaaatacat acattgtctt
3181 ccgtaaagcg ctagactata tattattata caggttcaaa tatactatct gtttcaggga
3241 aaactcccag gttcggatgt tcaaaattca atgatgggta acaagtacga tcgtaaatct
3301 gtaaaacagt ttgtcggata ttaggctgta tctcctcaaa gcgtattcga atatcattga
3361 gaagctgcag gcaagtgcac aaacaatact taaataaata ctactcagta ataacctatt
3421 tcttagcatt tttgacgaaa tttgctattt tgttagagtc ttttacacca tttgtctcca
3481 cacctccgct tacatcaaca ccaataacgc catttaatct aagcgcatca ccaacatttt
3541 ctggcgtcag tccaccagct aacataaaat gtaagctttc ggggctctct tgccttccaa
3601 cccagtcaga aatcgagttc caatccaaaa gttcacctgt cccacctgct tctgaatcaa
3661 acaagggaat aaacgaatga ggtttctgtg aagctgcact gagtagtatg ttgcagtctt
3721 ttggaaatac gagtctttta ataactggca aaccgaggaa ctcttggtat tcttgccacg
3781 actcatctcc atgcagttgg acgatatcaa tgccgtaatc attgaccaga gccaaaacat
3841 cctccttagg ttgattacga aacacgccaa ccaagtattt cggagtgcct gaactatttt
3901 tatatgcttt tacaagactt gaaattttcc ttgcaataac cgggtcaatt gttctctttc
3961 tattgggcac acatataata cccagcaagt cagcatcgga atctagagca cattctgcgg
4021 cctctgtgct ctgcaagccg caaactttca ccaatggacc agaactacct gtgaaattaa
4081 taacagacat actccaagct gcctttgtgt gcttaatcac gtatactcac gtgctcaata
4141 gtcaccaatg ccctccctct tggccctctc cttttctttt ttcgaccgaa tttcttgaag
4201 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
4261 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
4321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
4381 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
4441 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
4501 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
4561 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
4621 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
4681 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
4741 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
4801 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
4861 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
4921 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
4981 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
5041 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
5101 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
5161 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
5221 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
5281 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
5341 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
5401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
5461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
5521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
5581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
5641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
5701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
5761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
5821 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
5881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
5941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
6001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
6061 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
6121 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
6181 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
6241 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
6301 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
6361 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
6421 catgattacg aattaattcg agctcggta
//
