| 出品公司: | Clontech |
|---|---|
| 载体名称: | pEG202 |
| 质粒类型: | 酵母质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 10166 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEG202 10166 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Episomal yeast two-hybrid vector for constitutive expression of LexA
fused to the N-terminus of a protein. Also known as pLexA.
ACCESSION U89960
VERSION .
KEYWORDS pEG202
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10166)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Friday, February 14, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10166
/organism="synthetic DNA construct"
/lab_host="Saccharomyces cerevisiae"
/mol_type="other DNA"
promoter 1110..1511
/gene="S. cerevisiae ADH1"
/note="ADH1 promoter"
/note="promoter for alcohol dehydrogenase 1"
CDS 1538..2143
/codon_start=1
/gene="lexA"
/product="E. coli DNA-binding protein that represses genes
involved in the SOS response to DNA damage"
/note="LexA"
/note="The N-terminal DNA-binding domain recognizes the
16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
/translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
misc_feature 2144..2182
/note="MCS"
/note="multiple cloning site"
terminator 2209..2396
/gene="S. cerevisiae ADH1"
/note="ADH1 terminator"
/note="transcription terminator for alcohol dehydrogenase
1"
rep_origin 3128..4470
/note="2u ori"
/note="yeast 2u plasmid origin of replication"
CDS complement(6114..6773)
/codon_start=1
/gene="S. cerevisiae HIS3"
/product="imidazoleglycerol-phosphate dehydratase, required
for histidine biosynthesis"
/note="HIS3"
/note="yeast auxotrophic marker"
/translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
promoter complement(6774..6961)
/gene="S. cerevisiae HIS3"
/note="HIS3 promoter"
promoter 8026..8130
/gene="bla"
/note="AmpR promoter"
CDS 8131..8991
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 9162..9750
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggatgatccg
61 ggatcgaaga aatgatggta aatgaaatag gaaatcaagg agcatgaagg caaaagacaa
121 atataagggt cgaacgaaaa ataaagtgaa aagtgttgat atgatgtatt tggctttgcg
181 gcgccgaaaa aacgagttta cgcaattgca caatcatgct gactctgtgg cggacccgcg
241 ctcttgccgg cccggcgata acgctgggcg tgaggctgtg cccggcggag ttttttgcgc
301 ctgcattttc caaggtttac cctgcgctaa ggggcgagat tggagaagca ataagaatgc
361 cggttggggt tgcgatgatg acgaccacga caactggtgt cattatttaa gttgccgaaa
421 gaacctgagt gcatttgcaa catgagtata ctagaagaat gagccaagac ttgcgagacg
481 cgagtttgcc ggtggtgcga acaatagagc gaccatgacc ttgaaggtga gacgcgcata
541 accgctagag tactttgaag aggaaacagc aatagggttg ctaccagtat aaatagacag
601 gtacatacaa cactggaaat ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg
661 cactttatta tgttacaata tggaagggaa ctttacactt ctcctatgca catatattaa
721 ttaaagtcca atgctagtag agaagggggg taacacccct ccgcgctctt ttccgatttt
781 tttctaaacc gtggaatatt tcggatatcc ttttgttgtt tccgggtgta caatatggac
841 ttcctctttt ctggcaacca aacccataca tcgggattcc tataatacct tcgttggtct
901 ccctaacatg taggtggcgg aggggagata tacaatagaa cagataccag acaagacata
961 atgggctaaa caagactaca ccaattacac tgcctcattg atggtggtac ataacgaact
1021 aatactgtag ccctagactt gatagccatc atcatatcga agtttcacta ccctttttcc
1081 atttgccatc tattgaagta ataataggcg catgcaactt cttttctttt tttttctttt
1141 ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca aaaaaaatga tggaagacac
1201 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg
1261 aggggtatct tcgaacacac gaaacttttt ccttccttca ttcacgcaca ctactctcta
1321 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaagtttgc
1381 cgctttgcta tcaagtataa atagacctgc aattattaat cttttgtttc ctcgtcattg
1441 ttctcgttcc ctttcttcct tgtttctttt tctgcacaat atttcaagct ataccaagca
1501 tacaatcaac tccaagcttg aattaattcc gggcggaatg aaagcgttaa cggccaggca
1561 acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc cgccgacgcg
1621 tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag aacatctgaa
1681 ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg ggattcgtct
1741 gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg gtgaaccact
1801 tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttat tcaagccgaa
1861 tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca ttatggatgg
1921 tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg ttgtcgcacg
1981 tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag tcgaactgtt
2041 gccagaaaat agcgagttta aaccaattgt cgtagatctt cgtcagcaga gcttcaccat
2101 tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctggaattcc cggggatccg
2161 tcgaccatgg cggccgctcg agtcgacctg cagccaagct aattccgggc gaatttctta
2221 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
2281 agtgactctt aggttttaaa acgaaaattc ttgttcttga gtaactcttt cctgtaggtc
2341 aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg
2401 ccgagcaaat gcctgcaaat cgctccccat ttcacccaat tgtagatatg ctaactccag
2461 caatgagttg atgaatctcg gtgtgtattt tatgtcctca gaggacaaca cctgttgtaa
2521 tcgttcttcc acacggatcg atccacagga cgggtgtggt cgccatgatc gcgtagtcga
2581 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg
2641 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat
2701 agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca
2761 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat
2821 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc
2881 attaaagcta gctttgaaga aaaatgcgcc ttattcaatc tttgctataa aaaatggccc
2941 aaaatctcac attggaagac atttgatgac ctcatttctt tcaatgaagg gcctaacgga
3001 gttgactaat gttgtgggaa attggagcga taagcgtgct tctgccgtgg ccaggacaac
3061 gtatactcat cagataacag caatacctga tcactacttc gcactagttt ctcggtacta
3121 tgcatatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta atccaattga
3181 ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac gataccccgc
3241 atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca taaatagacg
3301 catataagta cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat
3361 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
3421 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
3481 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
3541 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
3601 taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc
3661 cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta
3721 cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat
3781 tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga
3841 gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca
3901 ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt
3961 tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc
4021 aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga
4081 cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag
4141 taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa
4201 aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa
4261 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta
4321 caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc
4381 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg
4441 ttctacaaaa tgaagcacag atgcttcgtt aacaaagata tgctattgaa gtgcaagatg
4501 gaaacgcaga aaatgaaccg gggatgcgac gtgcaagatt acctatgcaa tagatgcaat
4561 agtttctcca ggaaccgaaa tacatacatt gtcttccgta aagcgctaga ctatatatta
4621 ttatacaggt tcaaatatac tatctgtttc agggaaaact cccaggttcg gatgttcaaa
4681 attcaatgat gggtaacaag tacgatcgta aatctgtaaa acagtttgtc ggatattagg
4741 ctgtatctcc tcaaagcgta ttcgaatatc attgagaagc tgcagcaggc gtgaagttag
4801 acgacaactt ctctctggaa acgcataccg atattcaggc tgctgcaaag gcacaggcta
4861 gtgcccgtgc gagtgcatcc ggtaccaccc cagatgctgt agtagcttct ggtagcactg
4921 caatgagcca tgcttatcaa gaaaacacag gttttggtac tcgtcccata tatcttgaca
4981 tgcaagccac tacaccaaca gaccctaggg ttttggatac gatgttgaag ttttatacgg
5041 gactttatgg taatcctcat tccaacactc actcttacgg ttgggaaaca aatactgctg
5101 tggaaaatgc tagagctcac gtagcaaaga tgatcaatgc cgaccccaag gaaataatat
5161 tcacttcggg agcgaccgaa tctaataata tggttcttaa gggtgtccca agattttata
5221 agaagactaa gaaacacatc atcaccacta gaacggaaca caagtgtgtc ttggaagccg
5281 cacgggccat gatgaaggag ggatttgaag tcactttcct aaatgtggac gatcaaggtc
5341 ttatcgattt gaaggaattg gaagatgcca ttagaccaga tacctgtctc gtctctgtga
5401 tggctgtcaa taatgaaatc ggtgtcattc aacctattaa agaaattgga gcaatttgta
5461 gaaagaataa gatcctcggg gacaccaaat atggcgatct cggccttttc gtttcttgga
5521 gctgggacat gtttgccatc gatccatcta ccaccagaac ggccgttaga tctgctgcca
5581 ccgttgtttc caccgaagaa accaccgttg ccgtaaccac cacgacggtt gttgctaaag
5641 aagctgccac cgccacggcc accgttgtag ccgccgttgt tgttattgta gttgctactg
5701 ttatttctgg cacttcttgg ttttcctctt aagtgaggag gaacataacc attctcgttg
5761 ttgtcgttga tgcttaaatt ttgcacttgt tcgctcagtt cagccataat atgaaatgct
5821 tttcttgttg ttcttacgga ataccacttg ccacctatca ccacaactaa ctttttcccg
5881 ttcctccatc tcttttatat tttttttctc gatcgagttc aagagaaaaa aaaagaaaaa
5941 gcaaaaagaa aaaaggaaag cgcgcctcgt tcagaatgac acgtatagaa tgatgcatta
6001 ccttgtcatc ttcagtatca tactgttcgt atacatactt actgacattc ataggtatac
6061 atatatacac atgtatatat atcgtatgct gcagctttaa ataatcggtg tcactacata
6121 agaacacctt tggtggaggg aacatcgttg gtaccattgg gcgaggtggc ttctcttatg
6181 gcaaccgcaa gagccttgaa cgcactctca ctacggtgat gatcattctt gcctcgcaga
6241 caatcaacgt ggagggtaat tctgctagcc tctgcaaagc tttcaagaaa atgcgggatc
6301 atctcgcaag agagatctcc tactttctcc ctttgcaaac caagttcgac aactgcgtac
6361 ggcctgttcg aaagatctac caccgctctg gaaagtgcct catccaaagg cgcaaatcct
6421 gatccaaacc tttttactcc acgcgccagt agggcctctt taaaagcttg accgagagca
6481 atcccgcagt cttcagtggt gtgatggtcg tctatgtgta agtcaccaat gcactcaacg
6541 attagcgacc agccggaatg cttggccaga gcatgtatca tatggtccag aaaccctata
6601 cctgtgtgga cgttaatcac ttgcgattgt gtggcctgtt ctgctactgc ttctgcctct
6661 ttttctggga agatcgagtg ctctatcgct aggggaccac cctttaaaga gatcgcaatc
6721 tgaatcttgg tttcatttgt aatacgcttt actagggctt tctgctctgt catctttgcc
6781 ttcgtttatc ttgcctgctc attttttagt atattcttcg aagaaatcac attactttat
6841 ataatgtata attcattatg tgataatgcc aatcgctaag aaaaaaaaag agtcatccgc
6901 taggtggaaa aaaaaaaatg aaaatcatta ccgaggcata aaaaaatata gagtgtacta
6961 gaggaggcca agagtaatag aaaaagaaaa ttgcgggaaa ggactgtgtt atgacttccc
7021 tgactaatgc cgtgttcaaa cgatacctgg cagtgactcc tagcgctcac caagctctta
7081 aaacgagaat taagaaaaag tcgtcatctt tcgataagtt tttcccacag caaagcaata
7141 gtagaaaaac aatgggaaac gttgaatgaa gacaaagcgt cgtggtttaa aaggaaatac
7201 gctcacgtac atgctaggga acaggaccgt gcagcggatc taatgaatcc atttgttagt
7261 taatagttta aatgttttta tcggaagagg ttttgtcatc acatcagcaa tgttcttctt
7321 ggtctcgatg tagtatacgt ataaattatt acctgatact tcatctctaa gtctcattgc
7381 ctttgtgcca aaaaatctgt ttctaaattt ctcttcattt gtagacttaa ttatactgat
7441 cgttgatcta ctatcagtaa gtaagccttt aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
7501 acctgtaaca atagcaatac cccaaatacc taatgtagtt ccagcaagca agctaaaaag
7561 taaagcaaca acataactca cccctgcatc tgcagctttt gcccgggcag cctgctctgc
7621 ctgtgttttc tttaattgag cagtagacca tttagcagtt gcatgaatag ctgcagcgtc
7681 acatcggata ataatgatgg cagccattgt agaagtgcct tttgcatttc tagtctcttt
7741 ctcggtctag ctagttttac tacatcgcga agatagaatc ttagatcaca ctgcctttgc
7801 tgagctggat caatagagta acaaaagagt ggtaaggcct cgttaaagga caaggacctg
7861 agcggaagtg tatcgtacag tagacggagt atactagtat agtctatagt ccgtggaatt
7921 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
7981 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
8041 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
8101 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
8161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
8221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
8281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
8341 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
8401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
8461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
8521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
8581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
8641 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
8701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
8761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
8821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
8881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
8941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
9001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
9061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
9121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
9181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
9241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
9301 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
9361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
9421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
9481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
9541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
9601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
9661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
9721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
9781 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
9841 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
9901 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
9961 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
10021 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
10081 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
10141 acagacaagc tgtgaccgtc tccggg
//
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