出品公司: | Clontech |
---|---|
载体名称: | pEG202 |
质粒类型: | 酵母质粒 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | -- |
启动子: | -- |
载体大小: | 10166 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
买家导航
pEG202载体质粒基本信息
pEG202质粒图谱载体图谱和pEG202载体序列质粒序列多克隆位点信息
pEG202质粒载体简介
pEG202质粒序列载体序列
LOCUS pEG202 10166 bp ds-DNA circular SYN 19-OCT-2017 DEFINITION Episomal yeast two-hybrid vector for constitutive expression of LexA fused to the N-terminus of a protein. Also known as pLexA. ACCESSION U89960 VERSION . KEYWORDS pEG202 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10166) AUTHORS Clontech (TaKaRa) TITLE Direct Submission JOURNAL Exported Friday, February 14, 2020 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10166 /organism="synthetic DNA construct" /lab_host="Saccharomyces cerevisiae" /mol_type="other DNA" promoter 1110..1511 /gene="S. cerevisiae ADH1" /note="ADH1 promoter" /note="promoter for alcohol dehydrogenase 1" CDS 1538..2143 /codon_start=1 /gene="lexA" /product="E. coli DNA-binding protein that represses genes involved in the SOS response to DNA damage" /note="LexA" /note="The N-terminal DNA-binding domain recognizes the 16-bp palindromic sequence 5'-CTGTATATATATACAG-3'." /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL" misc_feature 2144..2182 /note="MCS" /note="multiple cloning site" terminator 2209..2396 /gene="S. cerevisiae ADH1" /note="ADH1 terminator" /note="transcription terminator for alcohol dehydrogenase 1" rep_origin 3128..4470 /note="2u ori" /note="yeast 2u plasmid origin of replication" CDS complement(6114..6773) /codon_start=1 /gene="S. cerevisiae HIS3" /product="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /note="HIS3" /note="yeast auxotrophic marker" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM" promoter complement(6774..6961) /gene="S. cerevisiae HIS3" /note="HIS3 promoter" promoter 8026..8130 /gene="bla" /note="AmpR promoter" CDS 8131..8991 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 9162..9750 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggatgatccg 61 ggatcgaaga aatgatggta aatgaaatag gaaatcaagg agcatgaagg caaaagacaa 121 atataagggt cgaacgaaaa ataaagtgaa aagtgttgat atgatgtatt tggctttgcg 181 gcgccgaaaa aacgagttta cgcaattgca caatcatgct gactctgtgg cggacccgcg 241 ctcttgccgg cccggcgata acgctgggcg tgaggctgtg cccggcggag ttttttgcgc 301 ctgcattttc caaggtttac cctgcgctaa ggggcgagat tggagaagca ataagaatgc 361 cggttggggt tgcgatgatg acgaccacga caactggtgt cattatttaa gttgccgaaa 421 gaacctgagt gcatttgcaa catgagtata ctagaagaat gagccaagac ttgcgagacg 481 cgagtttgcc ggtggtgcga acaatagagc gaccatgacc ttgaaggtga gacgcgcata 541 accgctagag tactttgaag aggaaacagc aatagggttg ctaccagtat aaatagacag 601 gtacatacaa cactggaaat ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg 661 cactttatta tgttacaata tggaagggaa ctttacactt ctcctatgca catatattaa 721 ttaaagtcca atgctagtag agaagggggg taacacccct ccgcgctctt ttccgatttt 781 tttctaaacc gtggaatatt tcggatatcc ttttgttgtt tccgggtgta caatatggac 841 ttcctctttt ctggcaacca aacccataca tcgggattcc tataatacct tcgttggtct 901 ccctaacatg taggtggcgg aggggagata tacaatagaa cagataccag acaagacata 961 atgggctaaa caagactaca ccaattacac tgcctcattg atggtggtac ataacgaact 1021 aatactgtag ccctagactt gatagccatc atcatatcga agtttcacta ccctttttcc 1081 atttgccatc tattgaagta ataataggcg catgcaactt cttttctttt tttttctttt 1141 ctctctcccc cgttgttgtc tcaccatatc cgcaatgaca aaaaaaatga tggaagacac 1201 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg 1261 aggggtatct tcgaacacac gaaacttttt ccttccttca ttcacgcaca ctactctcta 1321 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaagtttgc 1381 cgctttgcta tcaagtataa atagacctgc aattattaat cttttgtttc ctcgtcattg 1441 ttctcgttcc ctttcttcct tgtttctttt tctgcacaat atttcaagct ataccaagca 1501 tacaatcaac tccaagcttg aattaattcc gggcggaatg aaagcgttaa cggccaggca 1561 acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc cgccgacgcg 1621 tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag aacatctgaa 1681 ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg ggattcgtct 1741 gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg gtgaaccact 1801 tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttat tcaagccgaa 1861 tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca ttatggatgg 1921 tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg ttgtcgcacg 1981 tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag tcgaactgtt 2041 gccagaaaat agcgagttta aaccaattgt cgtagatctt cgtcagcaga gcttcaccat 2101 tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctggaattcc cggggatccg 2161 tcgaccatgg cggccgctcg agtcgacctg cagccaagct aattccgggc gaatttctta 2221 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa 2281 agtgactctt aggttttaaa acgaaaattc ttgttcttga gtaactcttt cctgtaggtc 2341 aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg 2401 ccgagcaaat gcctgcaaat cgctccccat ttcacccaat tgtagatatg ctaactccag 2461 caatgagttg atgaatctcg gtgtgtattt tatgtcctca gaggacaaca cctgttgtaa 2521 tcgttcttcc acacggatcg atccacagga cgggtgtggt cgccatgatc gcgtagtcga 2581 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg 2641 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat 2701 agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca 2761 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat 2821 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc 2881 attaaagcta gctttgaaga aaaatgcgcc ttattcaatc tttgctataa aaaatggccc 2941 aaaatctcac attggaagac atttgatgac ctcatttctt tcaatgaagg gcctaacgga 3001 gttgactaat gttgtgggaa attggagcga taagcgtgct tctgccgtgg ccaggacaac 3061 gtatactcat cagataacag caatacctga tcactacttc gcactagttt ctcggtacta 3121 tgcatatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta atccaattga 3181 ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac gataccccgc 3241 atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca taaatagacg 3301 catataagta cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat 3361 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg 3421 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc 3481 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag 3541 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa 3601 taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc 3661 cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta 3721 cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat 3781 tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga 3841 gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca 3901 ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt 3961 tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc 4021 aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga 4081 cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag 4141 taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa 4201 aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa 4261 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta 4321 caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc 4381 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg 4441 ttctacaaaa tgaagcacag atgcttcgtt aacaaagata tgctattgaa gtgcaagatg 4501 gaaacgcaga aaatgaaccg gggatgcgac gtgcaagatt acctatgcaa tagatgcaat 4561 agtttctcca ggaaccgaaa tacatacatt gtcttccgta aagcgctaga ctatatatta 4621 ttatacaggt tcaaatatac tatctgtttc agggaaaact cccaggttcg gatgttcaaa 4681 attcaatgat gggtaacaag tacgatcgta aatctgtaaa acagtttgtc ggatattagg 4741 ctgtatctcc tcaaagcgta ttcgaatatc attgagaagc tgcagcaggc gtgaagttag 4801 acgacaactt ctctctggaa acgcataccg atattcaggc tgctgcaaag gcacaggcta 4861 gtgcccgtgc gagtgcatcc ggtaccaccc cagatgctgt agtagcttct ggtagcactg 4921 caatgagcca tgcttatcaa gaaaacacag gttttggtac tcgtcccata tatcttgaca 4981 tgcaagccac tacaccaaca gaccctaggg ttttggatac gatgttgaag ttttatacgg 5041 gactttatgg taatcctcat tccaacactc actcttacgg ttgggaaaca aatactgctg 5101 tggaaaatgc tagagctcac gtagcaaaga tgatcaatgc cgaccccaag gaaataatat 5161 tcacttcggg agcgaccgaa tctaataata tggttcttaa gggtgtccca agattttata 5221 agaagactaa gaaacacatc atcaccacta gaacggaaca caagtgtgtc ttggaagccg 5281 cacgggccat gatgaaggag ggatttgaag tcactttcct aaatgtggac gatcaaggtc 5341 ttatcgattt gaaggaattg gaagatgcca ttagaccaga tacctgtctc gtctctgtga 5401 tggctgtcaa taatgaaatc ggtgtcattc aacctattaa agaaattgga gcaatttgta 5461 gaaagaataa gatcctcggg gacaccaaat atggcgatct cggccttttc gtttcttgga 5521 gctgggacat gtttgccatc gatccatcta ccaccagaac ggccgttaga tctgctgcca 5581 ccgttgtttc caccgaagaa accaccgttg ccgtaaccac cacgacggtt gttgctaaag 5641 aagctgccac cgccacggcc accgttgtag ccgccgttgt tgttattgta gttgctactg 5701 ttatttctgg cacttcttgg ttttcctctt aagtgaggag gaacataacc attctcgttg 5761 ttgtcgttga tgcttaaatt ttgcacttgt tcgctcagtt cagccataat atgaaatgct 5821 tttcttgttg ttcttacgga ataccacttg ccacctatca ccacaactaa ctttttcccg 5881 ttcctccatc tcttttatat tttttttctc gatcgagttc aagagaaaaa aaaagaaaaa 5941 gcaaaaagaa aaaaggaaag cgcgcctcgt tcagaatgac acgtatagaa tgatgcatta 6001 ccttgtcatc ttcagtatca tactgttcgt atacatactt actgacattc ataggtatac 6061 atatatacac atgtatatat atcgtatgct gcagctttaa ataatcggtg tcactacata 6121 agaacacctt tggtggaggg aacatcgttg gtaccattgg gcgaggtggc ttctcttatg 6181 gcaaccgcaa gagccttgaa cgcactctca ctacggtgat gatcattctt gcctcgcaga 6241 caatcaacgt ggagggtaat tctgctagcc tctgcaaagc tttcaagaaa atgcgggatc 6301 atctcgcaag agagatctcc tactttctcc ctttgcaaac caagttcgac aactgcgtac 6361 ggcctgttcg aaagatctac caccgctctg gaaagtgcct catccaaagg cgcaaatcct 6421 gatccaaacc tttttactcc acgcgccagt agggcctctt taaaagcttg accgagagca 6481 atcccgcagt cttcagtggt gtgatggtcg tctatgtgta agtcaccaat gcactcaacg 6541 attagcgacc agccggaatg cttggccaga gcatgtatca tatggtccag aaaccctata 6601 cctgtgtgga cgttaatcac ttgcgattgt gtggcctgtt ctgctactgc ttctgcctct 6661 ttttctggga agatcgagtg ctctatcgct aggggaccac cctttaaaga gatcgcaatc 6721 tgaatcttgg tttcatttgt aatacgcttt actagggctt tctgctctgt catctttgcc 6781 ttcgtttatc ttgcctgctc attttttagt atattcttcg aagaaatcac attactttat 6841 ataatgtata attcattatg tgataatgcc aatcgctaag aaaaaaaaag agtcatccgc 6901 taggtggaaa aaaaaaaatg aaaatcatta ccgaggcata aaaaaatata gagtgtacta 6961 gaggaggcca agagtaatag aaaaagaaaa ttgcgggaaa ggactgtgtt atgacttccc 7021 tgactaatgc cgtgttcaaa cgatacctgg cagtgactcc tagcgctcac caagctctta 7081 aaacgagaat taagaaaaag tcgtcatctt tcgataagtt tttcccacag caaagcaata 7141 gtagaaaaac aatgggaaac gttgaatgaa gacaaagcgt cgtggtttaa aaggaaatac 7201 gctcacgtac atgctaggga acaggaccgt gcagcggatc taatgaatcc atttgttagt 7261 taatagttta aatgttttta tcggaagagg ttttgtcatc acatcagcaa tgttcttctt 7321 ggtctcgatg tagtatacgt ataaattatt acctgatact tcatctctaa gtctcattgc 7381 ctttgtgcca aaaaatctgt ttctaaattt ctcttcattt gtagacttaa ttatactgat 7441 cgttgatcta ctatcagtaa gtaagccttt aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 7501 acctgtaaca atagcaatac cccaaatacc taatgtagtt ccagcaagca agctaaaaag 7561 taaagcaaca acataactca cccctgcatc tgcagctttt gcccgggcag cctgctctgc 7621 ctgtgttttc tttaattgag cagtagacca tttagcagtt gcatgaatag ctgcagcgtc 7681 acatcggata ataatgatgg cagccattgt agaagtgcct tttgcatttc tagtctcttt 7741 ctcggtctag ctagttttac tacatcgcga agatagaatc ttagatcaca ctgcctttgc 7801 tgagctggat caatagagta acaaaagagt ggtaaggcct cgttaaagga caaggacctg 7861 agcggaagtg tatcgtacag tagacggagt atactagtat agtctatagt ccgtggaatt 7921 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata 7981 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 8041 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 8101 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 8161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 8221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 8281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 8341 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt 8401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 8461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 8521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 8581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 8641 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa 8701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 8761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 8821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 8881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 8941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 9001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 9061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 9121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 9181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 9241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 9301 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 9361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 9421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 9481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 9541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 9601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 9661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 9721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 9781 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 9841 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 9901 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg 9961 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 10021 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 10081 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 10141 acagacaagc tgtgaccgtc tccggg //
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pEG202质粒载体应用举例
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