| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL2-Basic |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 5598 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: |
pGL2-Basic没有增强子或启动子。宿主：大肠杆菌JM109，哺乳动物。相关载体：f1，pBR322，pGL2-Enhancer， pGL2-Promoter,pGL2-Control。（信息来源：VectorDB（http://seq.yeastgome.org/VectorDB.））无启动子载体，用于用荧光素酶测定测定启动子和增强子序列的活性。
|
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pGL2Basic载体基本信息
pGL2Basic载体图谱质粒图谱和多克隆位点信息
pGL2Basic载体简介
pGL2Basic载体序列
LOCUS pGL2-Basic 5598 bp ds-DNA circular SYN 28-JUN-2013
DEFINITION Promoterless vector for measuring the activity of promoter and
enhancer sequences with a luciferase assay.
ACCESSION X65323
VERSION .
KEYWORDS pGL2-Basic
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5598)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Monday, February 3, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5598
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..52
/note="MCS"
/note="multiple cloning site"
CDS 76..1728
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/note="luciferase"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
intron 1969..2034
/note="small t intron"
/note="simian virus 40 (SV40) small t antigen intron"
polyA_signal 2609..2743
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3056..3644)
/direction=LEFT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3815..4675)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4676..4780)
/gene="bla"
/note="AmpR promoter"
rep_origin 4807..5262
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 5452..5573
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
ORIGIN
1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc ttggcattcc
61 ggtactgttg gtaaaatgga agacgccaaa aacataaaga aaggcccggc gccattctat
121 cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
181 gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa
241 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
301 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
361 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
421 aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa
481 attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa
541 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
601 tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata
661 atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc
721 tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
781 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
841 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga
901 tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc
961 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg
1021 ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg
1081 atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
1141 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
1201 ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct
1261 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
1321 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt
1381 gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa
1441 tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat
1501 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
1561 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
1621 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
1681 agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa tgtaactgta
1741 ttcagcgatg acgaaattct tagctattgt aatactgcga tgagtggcag ggcggggcgt
1801 aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg
1861 ataataagcg gatgaatggc agaaattcgc cggatctttg tgaaggaacc ttacttctgt
1921 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
1981 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
2041 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
2101 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
2161 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
2221 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
2281 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
2341 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
2401 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
2461 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
2521 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
2581 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
2641 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
2701 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgtcgac cgatgccctt
2761 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
2821 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
2881 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
2941 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
3001 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
3061 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
3121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
3181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
3241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
3301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
3361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
3421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
3481 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
3541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
3601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
3661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
3721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
3781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
3841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
3901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
3961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
4021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
4081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
4141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
4201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
4261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
4321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
4381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
4441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
4501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
4561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
4621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
4681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
4741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
4801 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
4861 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
4921 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
4981 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
5041 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
5101 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
5161 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
5221 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc
5281 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc
5341 taccatgata agtaagtaat attaaggtac gtggaggttt tacttgcttt aaaaaacctc
5401 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
5461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
5521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatggtact
5581 gtaactgagc taacataa
//
pGL2Basic载体图谱质粒图谱pdf版和相关资料下载
pGL2Basic载体质粒应用举例
- 上一篇：pQE-80L-Kan
- 下一篇：pOTB7-Src
