| 出品公司: | Qiagen |
|---|---|
| 载体名称: | pQE-80L-Kan |
| 质粒类型: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 4541 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氯霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
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pQE-80L-Kan载体质粒基本信息
pQE-80L-Kan质粒图谱载体图谱和pQE-80L-Kan载体序列质粒序列多克隆位点信息
pQE-80L-Kan质粒载体简介
pQE-80L-Kan质粒序列载体序列
LOCUS pQE-80L-Kan 4541 bp ds-DNA circular SYN 05-DEC-2013 DEFINITION Bacterial lacIq vector with a kanamycin resistance marker for expressing N-terminally 6xHis-tagged proteins. For other reading frames, use pQE-81L-Kan or pQE82L.html' target='_blank'>pQE-82L-Kan. ACCESSION . VERSION . KEYWORDS pQE-80L-Kan SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4541) AUTHORS Qiagen TITLE Direct Submission JOURNAL Exported Monday, February 3, 2020 from SnapGene Viewer 3.1.4 http://www.snapgene.com COMMENT Because this vector contains the lacIq gene, the pREP4 plasmid is not needed. FEATURES Location/Qualifiers source 1..4541 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" promoter 10..54 /note="T5 promoter" /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 30..46 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 62..78 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 101..106 /note="ribosome binding site" CDS 115..117 /codon_start=1 /product="start codon" /note="ATG" /translation="M" CDS 127..144 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" misc_feature 145..192 /note="MCS" /note="multiple cloning site" misc_feature 192..202 /note="stop codons" /note="stop codons in all three reading frames" terminator 208..302 /note="lambda t0 terminator" /note="transcription terminator from phage lambda" CDS 346..1005 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" terminator 1070..1156 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(1249..2331) /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(2332..2409) /gene="lacI (mutant)" /note="lacIq promoter" /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." rep_origin complement(2927..3515) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3622..4437) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" ORIGIN 1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca 61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga 121 ggatcgcatc accatcacca tcacggatcc gcatgcgagc tcggtacccc gggtcgacct 181 gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag 241 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga 301 atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc 361 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt 421 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta 481 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc 541 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg 601 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc 661 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg 721 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc 781 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac 841 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg 901 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt 961 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt 1021 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg 1081 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1141 cctgagtagg acaaatccgc cctctagatt acgtgcagtc gatgataagc tgtcaaacat 1201 gagaattgtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct 1261 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 1321 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag 1381 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg 1441 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc 1501 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt 1561 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac 1621 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc 1681 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag 1741 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc 1801 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact 1861 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc 1921 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg 1981 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat 2041 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg 2101 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc 2161 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac 2221 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg 2281 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac 2341 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc 2401 gatggtgtcg gaatttcggg cagcgttggg tcctggccac gggtgcgcat gatctagagc 2461 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 2521 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 2581 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat 2641 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 2701 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc 2761 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 2821 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 2881 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 2941 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 3001 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 3061 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 3121 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 3181 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 3241 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 3301 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 3361 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 3421 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 3481 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 3541 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg gcgttaaggg 3601 attttggtca tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt 3661 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga 3721 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac 3781 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga 3841 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt 3901 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa 3961 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg 4021 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat 4081 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc 4141 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg 4201 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct 4261 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat 4321 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc 4381 catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac 4441 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgacc atgacattaa 4501 cctataaaaa taggcgtatc acgaggccct ttcgtcttca c //
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pQE-80L-Kan质粒载体应用举例
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