pEarleyGate303

LOCUS       pEarleyGate 303        10382 bp ds-DNA     circular SYN 26-JUL-2016
DEFINITION  Gateway(R)-compatible promoterless plant transformation vector with 
            a C-terminal Myc tag.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector
            NTI(R) format
            http://www.snapgene.com
COMMENT     The sequence was corrected by inserting a G at position 6490.
COMMENT     VNTDATE|660096000|
COMMENT     VNTDBDATE|776390400|
COMMENT     VNTNAME|pEarleyGate 303|
COMMENT     VNTAUTHORNAME|Pikaard Lab|
FEATURES             Location/Qualifiers
     misc_feature    3..27
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      113..365
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(375..926)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase草丁膦（磷丝菌素） 
                     乙酰转移酶"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
     promoter        complement(932..1312)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    1364..1483
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1513..1543
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1597..2256
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
     CDS             2598..2903
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
     protein_bind    complement(2944..3068)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3070..3099
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
     terminator      3153..3860
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4104..4128
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5428..6057
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
     CDS             6486..7559
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7625..7819
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8163..8303
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8489..9077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9164..9958)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
ORIGIN
        1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
       61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt
      121 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
      181 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
      241 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
      301 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
      361 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
      421 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
      481 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
      541 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
      601 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
      661 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
      721 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
      781 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
      841 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
      901 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa
      961 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat
     1021 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt
     1081 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta
     1141 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc
     1201 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa
     1261 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga
     1321 attgaattct gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa
     1381 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1441 aaaacagact acataatact gtaaaacaca acatatccag tcatattggc ggccgcatta
     1501 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1561 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1801 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2221 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2281 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2341 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     2401 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2461 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2521 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2581 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2641 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2701 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2761 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     2821 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     2881 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     2941 gaccatagtg actggatatg ttgtgtttta cagcattatg tagtctgttt tttatgcaaa
     3001 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3061 aaagtggtgg aacagaaact gatctctgaa gaagatctgt gactaggtga gtctagagag
     3121 ttaattaaga cccgggacta gtccctagag tcctgcttta atgagatatg cgagacgcct
     3181 atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt
     3241 gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta
     3301 tcgtattttt atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat
     3361 gtacaatatt aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga
     3421 tagagcgcca caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag
     3481 cggcagaacc ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt
     3541 gccccagggg ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg
     3601 aactccggtt ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc
     3661 cgctctaccg aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg
     3721 acttgctgcc ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg
     3781 caggtcaaac cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac
     3841 aacatgtcga ggctcagcag gacctgcagg catgcaagct tggcactggc cgtcgtttta
     3901 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3961 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4021 cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc
     4081 cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     4141 agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     4201 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     4261 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     4321 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     4381 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     4441 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     4501 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     4561 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     4621 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     4681 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     4741 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     4801 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     4861 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     4921 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     4981 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     5041 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     5101 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     5161 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     5221 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     5281 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     5341 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     5401 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     5461 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     5521 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     5581 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     5641 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     5701 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     5761 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     5821 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     5881 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     5941 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     6001 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     6061 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     6121 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     6181 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     6241 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     6301 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     6361 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     6421 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     6481 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     6541 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     6601 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     6661 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     6721 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     6781 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     6841 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     6901 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     6961 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     7021 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     7081 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     7141 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     7201 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     7261 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     7321 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     7381 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     7441 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     7501 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     7561 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     7621 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     7681 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     7741 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     7801 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     7861 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     7921 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     7981 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     8041 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     8101 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     8161 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     8221 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     8281 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8341 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8401 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8461 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8521 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8581 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8641 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8701 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8761 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8821 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8881 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     8941 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9001 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9061 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9121 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     9181 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     9241 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     9301 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     9361 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     9421 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     9481 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     9541 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     9601 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     9661 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     9721 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     9781 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     9841 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     9901 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     9961 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    10021 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    10081 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    10141 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
    10201 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
    10261 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
    10321 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
    10381 gg
//