pEarleyGate302

LOCUS       pEarleyGate 302        10376 bp ds-DNA     circular SYN 26-JUL-2016
DEFINITION  Gateway(R)-compatible promoterless plant transformation vector with 
            a C-terminal FLAG tag.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector
            NTI(R) format
            http://www.snapgene.com
COMMENT     The sequence was corrected by inserting a G at position 6484.
COMMENT     VNTDATE|660096000|
COMMENT     VNTDBDATE|776390400|
COMMENT     VNTNAME|pEarleyGate 302|
COMMENT     VNTAUTHORNAME|Pikaard Lab|
FEATURES             Location/Qualifiers
     misc_feature    3..27
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      113..365
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(375..926)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase草丁膦（磷丝菌素） 
                     乙酰转移酶"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
     promoter        complement(932..1312)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    1364..1483
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1513..1543
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1597..2256
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
     CDS             2598..2903
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
     protein_bind    complement(2944..3068)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3070..3093
                     /codon_start=1
                     /product="FLAG epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
     terminator      3147..3854
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4098..4122
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5422..6051
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
     CDS             6480..7553
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7619..7813
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8157..8297
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8483..9071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9158..9952)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
ORIGIN
        1 gctggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
       61 cggacgtttt taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt
      121 atttgaaaat tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag
      181 ataaagccac gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg
      241 aatttctgac aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg
      301 ccccatatgc aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt
      361 ccaagattct gcagtcaaat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg
      421 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
      481 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
      541 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
      601 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc
      661 gactcggccg tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg
      721 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
      781 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
      841 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
      901 tcggccgggc gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa
      961 attcagatgc tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat
     1021 ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt
     1081 gctgtaaagc gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta
     1141 ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc
     1201 gaaattcagg cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa
     1261 aataaaaact gactcggata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga
     1321 attgaattct gcagtcgacg gtaccgcggg cccgggatca tcaacaagtt tgtacaaaaa
     1381 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1441 aaaacagact acataatact gtaaaacaca acatatccag tcatattggc ggccgcatta
     1501 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1561 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1621 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1681 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1741 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     1801 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     1861 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     1921 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1981 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2041 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2101 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2161 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2221 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2281 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2341 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     2401 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2461 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2521 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2581 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2641 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2701 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     2761 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     2821 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     2881 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     2941 gaccatagtg actggatatg ttgtgtttta cagcattatg tagtctgttt tttatgcaaa
     3001 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3061 aaagtggtgg actacaaaga cgatgacgac aaatgactag gtgagtctag agagttaatt
     3121 aagacccggg actagtccct agagtcctgc tttaatgaga tatgcgagac gcctatgatc
     3181 gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct
     3241 cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat
     3301 ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa
     3361 tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc
     3421 gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag
     3481 aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca
     3541 ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc
     3601 ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct
     3661 accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc
     3721 tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc
     3781 aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg
     3841 tcgaggctca gcaggacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt
     3901 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3961 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4021 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag
     4081 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     4141 tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     4201 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct
     4261 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc
     4321 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg
     4381 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga
     4441 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact
     4501 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga
     4561 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg
     4621 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg
     4681 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg
     4741 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt
     4801 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag
     4861 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc
     4921 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct
     4981 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc
     5041 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg
     5101 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat
     5161 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca
     5221 cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc
     5281 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt
     5341 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa
     5401 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca
     5461 ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt
     5521 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat
     5581 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc
     5641 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc
     5701 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
     5761 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
     5821 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
     5881 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
     5941 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
     6001 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
     6061 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
     6121 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
     6181 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
     6241 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
     6301 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
     6361 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
     6421 ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
     6481 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
     6541 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
     6601 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
     6661 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     6721 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     6781 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     6841 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     6901 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     6961 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     7021 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     7081 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     7141 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     7201 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     7261 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     7321 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     7381 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     7441 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     7501 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     7561 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg
     7621 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     7681 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     7741 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     7801 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     7861 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     7921 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     7981 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
     8041 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     8101 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     8161 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     8221 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     8281 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8881 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     8941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9121 actcacgtta agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa
     9181 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
     9241 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
     9301 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
     9361 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
     9421 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
     9481 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
     9541 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
     9601 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
     9661 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
     9721 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
     9781 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
     9841 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
     9901 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
     9961 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
    10021 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
    10081 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg
    10141 atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat
    10201 ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc
    10261 aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct
    10321 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctgg
//