| 出品人: | Fodor,B.D.(2004) |
|---|---|
| 载体名称: | pMHE7Tc |
| 质粒类型: | 广宿主载体；细菌表达载体；体外表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7/lac |
| 载体大小: | 6014 bp |
| 5' 测序引物及序列: | T7 promoter 5’TAATACGACTCACTATAGGG 3’ |
| 3' 测序引物及序列: | T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’ |
| 载体标签: | FLAG-tag、Strep-tag II (N-ter)、EK site |
| 载体抗性: | 四环素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pMHE7Tc载体质粒基本信息
pMHE7Tc质粒图谱载体图谱和pMHE7Tc载体序列质粒序列多克隆位点信息
pMHE7Tc质粒载体简介
pMHE7Tc质粒序列载体序列
LOCUS AY303671 6014 bp DNA circular SYN 10-FEB-2004
DEFINITION Broad host range expression vector pMHE7Tc, complete sequence.
ACCESSION AY303671
VERSION AY303671.1 GI:34766424
AUTHORS Fodor,B.D.
TITLE Modular broad-host-range expression vectors for the purification of
proteins and protein complexes and their application
JOURNAL Submitted (21-MAY-2003) Biophysics, BRC Hungarian Academy of
Sciences, Temesvari, Szeged, Csongrad 6701, Hungary
FEATURES Location/Qualifiers
source 1..6014
/organism="Broad host range expression vector pMHE7Tc"
/mol_type="other DNA"
/db_xref="taxon:244946"
primer_bind 189..207
/note="T7 promoter primer"
regulatory 189..205
/regulatory_class="promoter"
/note="T7 promoter"
misc_feature 206..231
/note="lac operator"
regulatory 262..268
/regulatory_class="ribosome_binding_site"
misc_feature 276..278
/note="start codon of fusion protein"
misc_feature 282..305
/note="FLAG-tag"
misc_feature 291..305
/note="enterokinase cleaveage site"
misc_feature 318..341
/note="Strep-tag II"
misc_feature 342..353
/note="factor-Xa cleaveage site"
primer_bind complement(452..469)
/note="T7 terminator primer"
regulatory 465..512
/regulatory_class="terminator"
/note="T7 terminator"
rep_origin 1144..1806
/note="pBBR1 oriV"
misc_feature 2800..3793
/note="RP4 mob, oriT"
regulatory complement(3883..3998)
/regulatory_class="terminator"
/note="T4 terminator"
gene complement(4304..5494)
/gene="tet"
CDS complement(4304..5494)
/gene="tet"
/codon_start=1
/transl_table=11
/product="tetracyclin resistance"
/protein_id="AAQ82544.1"
/db_xref="GI:34766425"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
regulatory 5879..5994
/regulatory_class="terminator"
/note="T4 terminator"
ORIGIN
1 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
61 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
121 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
181 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
241 aataattttg tttaacttta agaaggagat ataccatgga cgactacaag gacgacgacg
301 acaagctcga gggcaactgg agccatccgc agttcgagaa gatcgaggga aggcctgtcg
361 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccggc tgctaacaaa
421 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
481 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatcg
541 cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg
601 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgactgc
661 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct
721 ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
781 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
841 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
901 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
961 ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc
1021 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg
1081 gcgaggcggc tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa
1141 gtgctaccgg cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag
1201 aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt
1261 ttcggtcaag gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc
1321 ctcgccgggg ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag
1381 gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact
1441 gaacaccgac aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac
1501 gtaggccgac acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac
1561 caagtccttg actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt
1621 cttctggctg accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag
1681 cattgccgcc gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc
1741 cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt
1801 ggccatgctt atctccatgc ggtagggtgc cgcacggttg cggcaccatg cgcaatcagc
1861 tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca gtcaattaca
1921 gattttcttt aacctacgca atgagctatt gcggggggtg ccgcaatgag ctgttgcgta
1981 cccccctttt ttaagttgtt gatttttaag tctttcgcat ttcgccctat atctagttct
2041 ttggtgccca aagaagggca cccctgcggg gttcccccac gccttcggcg cggctccccc
2101 tccggcaaaa agtggcccct ccggggcttg ttgatcgact gcgcggcctt cggccttgcc
2161 caaggtggcg ctgccccctt ggaacccccg cactcgccgc cgtgaggctc ggggggcagg
2221 cgggcgggct tcgccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt
2281 caaggccaag ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc
2341 aaccggcaag cgaagcgcgc aggccgcagg ccggaggctt ttccccagag aaaattaaaa
2401 aaattgatgg ggcaaggccg caggccgcgc agttggagcc ggtgggtatg tggtcgaagg
2461 ctgggtagcc ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc
2521 agcttgtcca gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgctcgc
2581 ggccatcgtc cacatatcca cgggctggca agggagcgca gcgaccgcgc agggcgaagc
2641 ccggagagca agcccgtagg gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca
2701 aagtctagtg agtatactca agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc
2761 cccgtcgagc cggttggaca ccaaaaggga ggggcaggca tggcggcata cgcgatcatg
2821 cgatgcaaga agctggcgaa aatgggcaac gtggcggcca gtctcaagca cgcctaccgc
2881 gagcgcgaga cgcccaacgc tgacgccagc aggacgccag agaacgagca ctgggcggcc
2941 agcagcaccg atgaagcgat gggccgactg cgcgagttgc tgccagagaa gcggcgcaag
3001 gacgctgtgt tggcggtcga gtacgtcatg acggccagcc cggaatggtg gaagtcggcc
3061 agccaagaac agcaggcggc gttcttcgag aaggcgcaca agtggctggc ggacaagtac
3121 ggggcggatc gcatcgtgac ggccagcatc caccgtgacg aaaccagccc gcacatgacc
3181 gcgttcgtgg tgccgctgac gcaggacggc aggctgtcgg ccaaggagtt catcggcaac
3241 aaagcgcaga tgacccgcga ccagaccacg tttgcggccg ctgtggccga tctagggctg
3301 caacggggca tcgagggcag caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc
3361 ctggagcggc caccagtggg ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc
3421 tatgcaccgc agggattggc cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa
3481 gccgtggccg accggctgac aaaagcggtt cggcaggggt atgagcctgc cctacaggcc
3541 gccgcaggag cgcgtgagat gcgcaagaag gccgatcaag cccaagagac ggcccgagac
3601 cttcgggagc gcctgaagcc cgttctggac gccctggggc cgttgaatcg ggatatgcag
3661 gccaaggccg ccgcgatcat caaggccgtg ggcgaaaagc tgctgacgga acagcgggaa
3721 gtccagcgcc agaaacaggc ccagcgccag caggaacgcg ggcgcgcaca tttccccgaa
3781 aagtgccacc tggcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat
3841 ctcggcttga acgaattgtt aggtggcggt acttgggtcg atagctttat gcttgtaaac
3901 cgttttgtga aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag
3961 taatataatc tattaaaggt cattcaaaag gtcatccacc ggatcaattc ccctgctcgc
4021 gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct
4081 cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct
4141 cactgatccg ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg
4201 ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt
4261 cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc
4321 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa
4381 tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg
4441 gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat
4501 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg
4561 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga
4621 cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
4681 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
4741 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
4801 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
4861 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
4921 tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
4981 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
5041 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
5101 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
5161 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatccacagg acgggtgtgg
5221 tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc
5281 ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat
5341 atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca
5401 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc
5461 cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat
5521 ttaactgtga taaactaccg cattaaagct cgcgcagatc agttggaaga atttgtccac
5581 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc
5641 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
5701 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
5761 tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg
5821 cgaagtaatc gcaacatccg cattaaaatc tagcgagggc tttactaagc tgatccggtg
5881 gatgaccttt tgaatgacct ttaatagatt atattactaa ttaattgggg accctagagg
5941 tccccttttt tattttaaaa attttttcac aaaacggttt acaagcataa agctcaagga
6001 gatggcgccc aaca
//
