| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pLP/VSVG， pLP-VSVG， pLP VSVG |
| 质粒类型: | 哺乳动物细胞慢病毒包装载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5821 bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 备注: |
载体使用Stbl3感受态细胞扩增质粒，可以减少载体基因重组几率。pLP/VSVG, pLP1, pLP2载体是Invitrogen公司出品的配套使用的慢病毒包装载体。 第三代慢病毒包装系统组成成份有pLP1、pLP2和pLP/VSVG（或者pCMV-VSVG）。在使用的时候，按照pLP1(3):pLP2(2):pLP/VSVG(3)的比例配成1 ug/ul的第三代慢病毒包装系统package Mix。转染时，对于60mm的细胞培养皿来说，按照慢病毒基因质粒（3ug）和package mix（6 ug，也就是6 ul）的比例进行慢病毒质粒包装实验。 |
| 产品目录号: | K4970-00 |
| 稳定性: | / |
| 组成型: | -- |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLP/VSVG载体质粒基本信息
pLP/VSVG质粒图谱载体图谱和pLP/VSVG载体序列质粒序列多克隆位点信息
pLP/VSVG质粒载体简介
pLP/VSVG质粒序列载体序列
LOCUS pLP/VSVG 5821 bp ds-DNA circular SYN 10-一月-2013
DEFINITION Lentiviral packaging plasmid for expression of the vesicular
stomatitis virus G glycoprotein.
ACCESSION .
VERSION .
KEYWORDS pLP VSVG
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..5821
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 79..458
/label="CMV enhancer"
/label="human cytomegalovirus immediate early enhancer"
promoter 459..662
/label="CMV promoter"
/label="human cytomegalovirus (CMV) immediate early
promoter"
intron 796..1271
/label="beta-globin intron"
/label="internally truncated intron from human?beta-globin"
CDS 1346..2881
/codon_start=1
/gene="
"
/product="vesicular stomatitis virus G glycoprotein"
/label="VSV-G"
/label="Indiana strain"
/protein_id="
"
polyA_site 3082..3476
/label="beta-globin poly(A)"
/label="human?beta-globin polyadenylation signal"
rep_origin complement(3986..4574)
/direction=LEFT
/label="ori"
/label="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4745..5605)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label="AmpR"
/label="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(5606..5710)
/gene="bla"
/label="AmpR promoter"
ORIGIN
1 ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
61 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
301 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
661 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
721 gaagacaccg ggaccgatcc agcctcccct cgaagcttac atgtggtacc gagctcggat
781 cctgagaact tcagggtgag tctatgggac ccttgatgtt ttctttcccc ttcttttcta
841 tggttaagtt catgtcatag gaaggggaga agtaacaggg tacacatatt gaccaaatca
901 gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt
961 tatcttattt ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta
1021 tcatgcctct ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag
1081 caatatttct gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat
1141 attgctaata gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag
1201 gctggattat tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct
1261 tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag
1321 cacgtgagat ctgaattctg acactatgaa gtgccttttg tacttagcct ttttattcat
1381 tggggtgaat tgcaagttca ccatagtttt tccacacaac caaaaaggaa actggaaaaa
1441 tgttccttct aattaccatt attgcccgtc aagctcagat ttaaattggc ataatgactt
1501 aataggcaca gccttacaag tcaaaatgcc caagagtcac aaggctattc aagcagacgg
1561 ttggatgtgt catgcttcca aatgggtcac tacttgtgat ttccgctggt atggaccgaa
1621 gtatataaca cattccatcc gatccttcac tccatctgta gaacaatgca aggaaagcat
1681 tgaacaaacg aaacaaggaa cttggctgaa tccaggcttc cctcctcaaa gttgtggata
1741 tgcaactgtg acggatgccg aagcagtgat tgtccaggtg actcctcacc atgtgctggt
1801 tgatgaatac acaggagaat gggttgattc acagttcatc aacggaaaat gcagcaatta
1861 catatgcccc actgtccata actctacaac ctggcattct gactataagg tcaaagggct
1921 atgtgattct aacctcattt ccatggacat caccttcttc tcagaggacg gagagctatc
1981 atccctggga aaggagggca cagggttcag aagtaactac tttgcttatg aaactggagg
2041 caaggcctgc aaaatgcaat actgcaagca ttggggagtc agactcccat caggtgtctg
2101 gttcgagatg gctgataagg atctctttgc tgcagccaga ttccctgaat gcccagaagg
2161 gtcaagtatc tctgctccat ctcagacctc agtggatgta agtctaattc aggacgttga
2221 gaggatcttg gattattccc tctgccaaga aacctggagc aaaatcagag cgggtcttcc
2281 aatctctcca gtggatctca gctatcttgc tcctaaaaac ccaggaaccg gtcctgcttt
2341 caccataatc aatggtaccc taaaatactt tgagaccaga tacatcagag tcgatattgc
2401 tgctccaatc ctctcaagaa tggtcggaat gatcagtgga actaccacag aaagggaact
2461 gtgggatgac tgggcaccat atgaagacgt ggaaattgga cccaatggag ttctgaggac
2521 cagttcagga tataagtttc ctttatacat gattggacat ggtatgttgg actccgatct
2581 tcatcttagc tcaaaggctc aggtgttcga acatcctcac attcaagacg ctgcttcgca
2641 acttcctgat gatgagagtt tattttttgg tgatactggg ctatccaaaa atccaatcga
2701 gcttgtagaa ggttggttca gtagttggaa aagctctatt gcctcttttt tctttatcat
2761 agggttaatc attggactat tcttggttct ccgagttggt atccatcttt gcattaaatt
2821 aaagcacacc aagaaaagac agatttatac agacatagag atgaaccgac ttggaaagta
2881 actcaaatcc tgcacaacag attcttcatg tttggaccaa atcaacttgt gataccatgc
2941 tcaaagaggc ctcaattata tttgagtttt taatttttat gaaaaaaaaa aaaaaaaacg
3001 gaattcaccc caccagtgca ggctgcctat cagaaagtgg tggctggtgt ggctaatgcc
3061 ctggcccaca agtatcacta agctcgcttt cttgctgtcc aatttctatt aaaggttcct
3121 ttgttcccta agtccaacta ctaaactggg ggatattatg aagggccttg agcatctgga
3181 ttctgcctaa taaaaaacat ttattttcat tgcaatgatg tatttaaatt atttctgaat
3241 attttactaa aaagggaatg tgggaggtca gtgcatttaa aacataaaga aatgaagagc
3301 tagttcaaac cttgggaaaa tacactatat cttaaactcc atgaaagaag gtgaggctgc
3361 aaacagctaa tgcacattgg caacagcccc tgatgcctat gccttattca tccctcagaa
3421 aaggattcaa gtagaggctt gatttggagg ttaaagtttt gctatgctgt attttacatt
3481 acttattgtt ttagctgtcc tcatgaatgt cttttcacta cccatttgct tatcctgcat
3541 ctctcagcct tgactccact cagttctctt gcttagagat accacctttc ccctgaagtg
3601 ttccttccat gttttacggc gagatggttt ctcctcgcct ggccactcag ccttagttgt
3661 ctctgttgtc ttatagaggt ctacttgaag aaggaaaaac agggggcatg gtttgactgt
3721 cctgtgagcc cttcttccct gcctccccca ctcacagtga cccggaatcc ctcgacatgg
3781 cagtctagca ctagtgcggc cgcagatctg cttcctcgct cactgactcg ctgcgctcgg
3841 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
3901 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
3961 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
4021 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
4081 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
4141 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
4201 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
4261 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
4321 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
4381 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
4441 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
4501 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
4561 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
4621 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
4681 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
4741 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
4801 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
4861 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
4921 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
4981 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
5041 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
5101 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
5161 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
5221 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
5281 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
5341 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
5401 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
5461 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
5521 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
5581 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
5641 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
5701 gggttccgcg cacatttccc cgaaaagtgc cacctgacgg gatcccctga gggggccccc
5761 atgggctaga ggatccggcc tcggcctctg cataaataaa aaaaattagt cagccatgag
5821 c
//
