| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pLP2 |
| 质粒类型: | 哺乳动物细胞慢病毒表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | RSV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4180 bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 备注: |
载体使用Stbl3感受态细胞扩增质粒，可以减少载体基因重组几率。pLP/VSVG, pLP1, pLP2载体是Invitrogen公司出品的配套使用的慢病毒包装载体。 第三代慢病毒包装系统组成成份有pLP1、pLP2和pLP/VSVG（或者pCMV-VSVG）。在使用的时候，按照pLP1(3):pLP2(2):pLP/VSVG(3)的比例配成1 ug/ul的第三代慢病毒包装系统package Mix。转染时，对于60mm的细胞培养皿来说，按照慢病毒基因质粒（3ug）和package mix（6 ug，也就是6 ul）的比例进行慢病毒质粒包装实验。 |
| 产品目录号: | K4970-00 |
| 稳定性: | / |
| 组成型: | -- |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported File 4180 bp ds-DNA circular SYN 10-一月-2013
DEFINITION Lentiviral packaging plasmid containing the HIV-1 rev gene.
ACCESSION .
VERSION .
KEYWORDS pLP2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4180
/organism="synthetic DNA construct"
/lab_host="M"
/mol_type="other DNA"
promoter 1..262
/note="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
CDS 391..741
/codon_start=1
/product="HIV-1 Rev protein"
/note="rev"
polyA_signal 850..971
/note="HIV-1 poly(A)"
/note="polyadenylation signal from the LTR of HIV-1"
rep_origin 1173..1628
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1910..2014
/gene="bla"
/note="AmpR promoter"
CDS 2015..2875
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3046..3634
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
181 ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc
241 attcaccaca ttggtgtgca cctccaagct cgagctcgtt tagtgaaccg tcagatcgcc
301 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
361 ccctcgaagc tagtcgatta ggcatctcct atggcaggaa gaagcggaga cagcgacgaa
421 gacctcctca aggcagtcag actcatcaag tttctctatc aaagcaaccc acctcccaat
481 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
541 cagatccatt cgattagtga acggatcctt agcacttatc tgggacgatc tgcggagcct
601 gtgcctcttc agctaccacc gcttgagaga cttactcttg attgtaacga ggattgtgga
661 acttctggga cgcagggggt gggaagccct caaatattgg tggaatctcc tacaatattg
721 gagtcaggag ctaaagaata gtgctgttag cttgctcaat gccacagcta tagcagtagc
781 tgaggggaca gatagggtta tagaagtagt acaagaagct tggcactggc cgtcgtttta
841 caacgtcgtg atctgagcct gggagatctc tggctaacta gggaacccac tgcttaagcc
901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
961 taactagaga tcaggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
1021 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
1081 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
1141 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
1201 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
1261 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
1321 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
1381 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
1441 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
1501 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg
1561 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
1621 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
1681 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
1741 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
1801 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
1861 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
1921 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
1981 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
2041 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
2101 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
2161 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
2221 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
2281 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
2341 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
2401 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
2461 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
2521 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
2581 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
2641 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
2701 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
2761 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
2821 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
2881 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
2941 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
3001 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
3061 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
3121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
3181 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
3241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
3301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
3361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
3421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
3481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
3541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
3601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
3661 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
3721 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
3781 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
3841 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
3901 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
3961 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
4021 aggaaacagc tatgacatga ttacgaattc gatgtacggg ccagatatac gcgtatctga
4081 ggggactagg gtgtgtttag gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc
4141 ctcaggatat agtagtttcg cttttgcata gggaggggga
//
