| 质粒名称: | pJFRC7-20XUAS-IVS-mCD8::GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26220 |
| 存储实验室: | Gerald Rubin |
|
载体骨架基本信息 |
|
| 载体名称: | pJFRC-MUH |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 8151 bp |
| 完整质粒大小: | 9540 bp |
| 载体类型: | 昆虫细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mCD8::GFP |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1389 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI(未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | hsp70 F: GAGCGCCGGAGTATAAATAGAG |
| 3' 测序引物 | SV40 R: CCATTCATCAGTTCCATAGG |
|
文献引用方法 |
|
| 材料和方法部分: | pJFRC7-20XUAS-IVS-mCD8::GFP was a gift from Gerald Rubin (爱迪基因 plasmid # 26220) |
| 参考文献部分: | Refinement of Tools for Targeted Gene Expression in Drosophila. Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW, Rubin GM. Genetics. 2010 Aug 9. ():. 10.1534/genetics.110.119917 PubMed 20697123 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJFRC7-20XUAS-IVS-mCD8::GFP 9540 bp ds-DNA circular SYN 10-JAN-
2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pJFRC7-20XUAS-IVS-mCD8::GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9540)
AUTHORS Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW,
Rubin GM
TITLE Refinement of Tools for Targeted Gene Expression in Drosophila.
JOURNAL Genetics. 2010 Aug 9. ():.
PUBMED 20697123
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pJFRC7-20XUAS-IVS-mCD8::GFP|
FEATURES Location/Qualifiers
source 1..9540
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 31..135
/gene="bla"
/label=AmpR promoter
CDS 136..996
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(354..373)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1167..1755
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1656..1675
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
protein_bind 1842..1911
/label=attB
/bound_moiety="phage phi-C31 integrase"
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
primer_bind complement(2136..2154)
/label=pCasper-F
/note="Drosophila mini-white gene, reverse primer"
CDS join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
4864..5478)
/codon_start=1
/gene="white"
/product="Drosophila white gene eye color pigment"
/label=mini-white
/note="This is a modified version of the white gene lacking
part of the first intron."
/translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
protein_bind 6508..6602
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
protein_bind 6636..6730
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
protein_bind 6764..6858
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
protein_bind 6892..6986
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 7011..7249
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
primer_bind 7196..7217
/label=pCasper-hs
/note="Drosophila Hsp70 promoter, forward primer"
intron 7264..7330
/label=Mhc intron
/note="modified Drosophila myosin heavy chain intron 16
(Pfeiffer et al., 2010)"
CDS 7351..8016
/codon_start=1
/product="mouse lymphocyte antigen CD8 alpha chain"
/label=mCD8
/translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
"
CDS 8023..8739
/codon_start=1
/product="Aequoria victoria green fluorescent protein"
/label=GFP
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(8053..8081)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 8676..8696
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
intron 8823..8888
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9018..9038
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 9310..9444
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9347..9366)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9401..9420
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9486..9504)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag
2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga
3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
6721 gtcctccgag cggagactct agcgctagcg catgcctgca ggtcggagta ctgtcctccg
6781 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
6841 ggagtactgt cctccgagcg gagactctag cactagtgca tgcctgcagg tcggagtact
6901 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
6961 ctccgagcgg agtactgtcc tccgagcgga gactctagcg acgtcgagcg ccggagtata
7021 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
7081 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
7141 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
7201 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctaa
7261 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc
7321 tttacttcag gcggccgcgg ctcgagcaaa atggcctcac cgttgacccg ctttctgtcg
7381 ctgaacctgc tgctgctggg tgagtcgatt atcctgggga gtggagaagc taagccacag
7441 gcacccgaac tccgaatctt tccaaagaaa atggacgccg aacttggtca gaaggtggac
7501 ctggtatgtg aagtgttggg gtccgtttcg caaggatgct cttggctctt ccagaactcc
7561 agctccaaac tcccccagcc caccttcgtt gtctatatgg cttcatccca caacaagata
7621 acgtgggacg agaagctgaa ttcgtcgaaa ctgttttctg ccatgaggga cacgaataat
7681 aagtacgttc tcaccctgaa caagttcagc aaggaaaacg aaggctacta tttctgctca
7741 gtcatcagca actcggtgat gtacttcagt tctgtcgtgc cagtccttca gaaagtgaac
7801 tctactacta ccaagccagt gctgcgaact ccctcacctg tgcaccctac cgggacatct
7861 cagccccaga gaccagaaga ttgtcggccc cgtggctcag tgaaggggac cggattggac
7921 ttcgcctgtg atatttacat ctgggcaccc ttggccggaa tctgcgtggc ccttctgctg
7981 tccttgatca tcactctcat ctgctaccac agccgcggat ccatgagtaa aggagaagaa
8041 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
8101 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
8161 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttaacttat
8221 ggtgttcaat gcttttcaag atacccagat catatgaaac agcatgactt tttcaagagt
8281 gccatgcccg aaggttatgt ccaggaaaga actatatttt tcaaagatga cgggaactac
8341 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
8401 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
8461 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
8521 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
8581 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
8641 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
8701 gctgggatta cacatggcat ggatgaacta tacaaataat ctagaggatc tttgtgaagg
8761 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
8821 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
8881 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
8941 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
9001 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
9061 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
9121 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
9181 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
9241 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
9301 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
9361 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
9421 aatgtatctt atcatgtctg gatcgatctg gccggccgtt taaacgaatt cttgaagacg
9481 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
//
