| 质粒名称: | MIGR1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27490 |
| 存储实验室: | Warren Pear |
|
载体骨架基本信息 |
|
| 载体名称: | MSCV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 6200 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: |
IRES（插入式C端子）
GFP（插入件上的C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pLXSN 5' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | MIGR1 was a gift from Warren Pear (Addgene plasmid # 27490 ; http://n2t.net/addgene:27490 ; RRID:Addgene_27490) |
| 参考文献部分: | Efficient and rapid induction of a chronic myelogenous leukemia-like myeloproliferative disease in mice receiving P210 bcr/abl-transduced bone marrow. Pear WS, Miller JP, Xu L, Pui JC, Soffer B, Quackenbush RC, Pendergast AM, Bronson R, Aster JC, Scott ML, Baltimore D. Blood. 1998 Nov 15. 92(10):3780-92. PubMed 9808572 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS MIGR1 6441 bp ds-DNA circular SYN 07-NOV-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS MIGR1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6441)
AUTHORS Pear WS, Miller JP, Xu L, Pui JC, Soffer B, Quackenbush RC,
Pendergast AM, Bronson R, Aster JC, Scott ML, Baltimore D
TITLE Efficient and rapid induction of a chronic myelogenous leukemia-like
myeloproliferative disease in mice receiving P210 bcr/abl-transduced
bone marrow.
JOURNAL Blood. 1998 Nov 15. 92(10):3780-92.
PUBMED 9808572
REFERENCE 2 (bases 1 to 6441)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6441
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 58..80
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 96..112
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 174..726
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(318..335)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 545..564
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 738..1457
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(783..804)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1044..1063)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1391..1412
/label=EGFP-C
/note="EGFP, forward primer"
LTR 1559..2073
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 2235..2251
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2259..2289)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2304..2325
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2442..2459)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2613..3201)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2693..2712)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3372..4232)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3995..4014
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4233..4337)
/gene="bla"
/label=AmpR promoter
primer_bind 4405..4423
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(4461..4483)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 4583..4602
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4796..4818
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 4946..4965
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 5165..5681
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 5745..6086
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 6153..128
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
ORIGIN
1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
61 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
121 taggcgccgg aattagatct ctcgaggtta acgaattccg cccccccccc ctaacgttac
181 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
241 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
301 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
361 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
421 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
481 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
541 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
601 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
661 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
721 ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
781 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtct ggcgagggcg
841 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
901 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
961 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
1021 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
1081 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
1141 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
1201 ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac atcgaggacg
1261 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
1321 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
1381 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
1441 acgagctgta caagtaatga attaattaag aattatcaag cttatcgata ccgtcgacct
1501 gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
1561 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
1621 gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag
1681 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
1741 cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc
1801 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
1861 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
1921 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa
1981 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
2041 gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa
2101 caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt
2161 gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg
2221 ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
2281 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
2341 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
2401 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
2461 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
2521 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
2581 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
2641 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
2701 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
2761 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
2821 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
2881 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
2941 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
3001 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
3061 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
3121 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
3181 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
3241 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
3301 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
3361 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
3421 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
3481 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
3541 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
3601 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
3661 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
3721 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
3781 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
3841 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
3901 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
3961 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
4021 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
4081 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
4141 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
4201 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
4261 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
4321 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
4381 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
4441 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
4501 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
4561 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
4621 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
4681 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
4741 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
4801 tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg gcgcccaaca
4861 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
4921 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
4981 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta gtccaatttg
5041 ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
5101 agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
5161 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
5221 aggcatggaa aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag
5281 acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
5341 ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag
5401 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
5461 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
5521 caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtattc
5581 ccaataaagc ctcttgctgt ttgcatccga atcgtggact cgctgatcct tgggagggtc
5641 tcctcagatt gattgactgc ccacctcggg ggtctttcat ttggaggttc caccgagatt
5701 tggagacccc tgcccaggga ccaccgaccc ccccgccggg aggtaagctg gccagcggtc
5761 gtttcgtgtc tgtctctgtc tttgtgcgtg tttgtgccgg catctaatgt ttgcgcctgc
5821 gtctgtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt
5881 tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg
5941 gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg
6001 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga
6061 accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc
6121 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt
6181 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
6241 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
6301 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
6361 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
6421 ttgacccccc tccctgggtc a
//
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