| 质粒名称: | pJRD215 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 167555 |
| 存储实验室: | Renato Morona |
|
载体骨架基本信息 |
|
| 载体名称: | pJRD215 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 10200 bp |
| 载体修饰: | -- |
| 载体类型: | 广泛的主机范围可移动 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pJRD215 was a gift from Renato Morona (Addgene plasmid # 167555 ; http://n2t.net/addgene:167555 ; RRID:Addgene_167555) |
| 参考文献部分: | Characterization of the rfc region of Shigella flexneri. Morona R, Mavris M, Fallarino A, Manning PA. J Bacteriol. 1994 Feb;176(3):733-47. doi: 10.1128/jb.176.3.733-747.1994. 10.1128/jb.176.3.733-747.1994 PubMed 7507920 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJRD215 10321 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Backbone plasmid to pRMA154.
ACCESSION .
VERSION .
KEYWORDS pJRD215
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10321)
AUTHORS Morona R, Mavris M, Fallarino A, Manning PA
TITLE Characterization of the rfc region of Shigella flexneri.
JOURNAL J Bacteriol. 1994 Feb;176(3):733-47. doi:
10.1128/jb.176.3.733-747.1994.
PUBMED 7507920
REFERENCE 2 (bases 1 to 10321)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10321
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 478..506
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
rep_origin 3001..3395
/label=RSF1010 oriV
/note="replication origin of the broad-host-range plasmid
RSF1010; requires the RSF1010 RepA/B/C proteins for
replication (Scholz et al., 1989)"
oriT 3737..3824
/label=RSF1010 oriT
/note="origin of transfer of the broad-host-range plasmid
RSF1010 (Scholz et al., 1989)"
CDS 5063..6034
/codon_start=1
/gene="repB"
/product="replication protein B of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
/label=RSF1010 RepB
/translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
CDS 6545..7384
/codon_start=1
/gene="repA"
/product="replication protein A of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
/label=RSF1010 RepA
/translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
CDS complement(8744..9538)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8855..8874)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 9465..9484
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
misc_feature 9922..10320
/label=cos
/note="lambda cos site; allows packaging into phage lambda
particles"
ORIGIN
1 cccccccccc ccccccctgc aggtcgacgg atccccgggt accataagta gaagcagcaa
61 cccaagtagc tttaccagca tccggttcaa ccagcatagt aagaatctta ctggacatcg
121 gcagttcttc gaacagtgcg ccaactacca gctctttctg cagttcattc agggcaccgg
181 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg
241 atccatggat tacgcgttaa cccgggcccg cggatgcata tgatcttaag gcctaggtct
301 agagtctttg ttttgacgcc attagcgtac gtaacaatcc tcgttaaagg acaaggacct
361 gagcggaagt gtatcgtaca gtagacggag tatactagta tagtctatag tccgtggaat
421 tattatattt atctccgacg atattctcat cagtgaaatc caggggaatt ctcatgtttg
481 acagcttatc atcgataagc tttaatgcgg tagtttatca cagttaaatt gctaacgcag
541 tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct
601 ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggataactg
661 cacattcggg atatttctct atattcgcgc ttcatcagaa aactgaagga acctccattg
721 aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt cagaggcgga
781 gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa aatcgcacct
841 gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct caaaggtcga
901 ggtgtggctt gccccgaggt catcaactgg caggaggaac aggagggtgc atgcttggtg
961 ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt gctcaaagcg
1021 tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga tcaatgtccg
1081 tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc ccgcaatgcc
1141 gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga tcttttggct
1201 cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgatat ggttgtttgc
1261 catggtgatc cctgcatgcc gaacttcatg gtggacccta aaactcttca atgcacgggt
1321 ctgatcgacc ttgggcggct cggaacagca gatcgctatg ccgatttggc actcatgatt
1381 gctaacgccg aagagaactg ggcagcgcca gatgaagcag agcgcgcctt cgctgtccta
1441 ttcaatgtat tggggatcga agcccccgac cgcgaacgcc ttgccttcta tctgcgattg
1501 gaccctctga cttggggttg atgttcatgc cgcctgtttt tcctgctcat tggcacgttt
1561 cgcaacctgt tctcattgcg gacacctttt ccagcctcgt ttggaaagtt tcattgccag
1621 acgggactcc tgcaatcgtc aagggattga aacctataga agacattgct gatgaactgc
1681 gcggggccga ctatctggta tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg
1741 agaacaatct gatgttgctc gaatatgccg gggagcgaat gctctctcac atcgttgccg
1801 agcacggcga ctaccaggcg accgaaattg cagcggaact aatggcgaag ctgtatgccg
1861 catctgagga acccctgcct tctgcccttc tcccgatccg ggatcgcttt gcagctttgt
1921 ttcagcgggc gcgcgatgat caaaacgcag gttgtcaaac tgactacgtc cacgcggcga
1981 ttatagccga tcaaatgatg agcaatgcct cggaactgcg tgggctacat ggcgatctgc
2041 atcatgaaaa catcatgttc tccagtcgcg gctggctggt gatagatccc gtcggtctgg
2101 tcggtgaagt gggctttggc gccgccaata tgttctacga tccggctgac agagacgacc
2161 tttgtctcga tcctagacgc attgcacaga tggcggacgc attctctcgt gcgctggacg
2221 tcgatccgcg tcgcctgctc gaccaggcgt acgcttatgg gtgcctttcc gcagcttgga
2281 acgcggatgg agaagaggag caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc
2341 gacagacgtc atactagata tcaagcgact tctcctatcc cctgggaaca catcaatctc
2401 accggagaat atcgctggcc aaagccttag cgtaggattc cgccccttcc cgcaaacgac
2461 cccaaacagg aaacgcagct gaaacgggaa gctcaacacc cactgacgca tgggttgttc
2521 aggcagtact tcatcaacca gcaaggcggc actttcggcc atccgccgcg ccccacagct
2581 cgggcagaaa ccgcgacgct tacagctgaa agcgaccagg tgctcggcgt ggcaagactc
2641 gcagcgaacc cgtagaaagc catgctccag ccgcccgcat tggagaaatt cttcaaattc
2701 ccgttgcaca tagcccggca attcctttcc ctgctctgcc ataagcgcag cgaatgccgg
2761 gtaatactcg tcaacgatct gatagagaag ggtttgctcg ggtcggtggc tctggtaacg
2821 accagtatcc cgatcccggc tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct
2881 tgcaatactg tgtttacata cagtctatcg cttagcggaa agttctttta ccctcagccg
2941 aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccgta ctaactgtca
3001 cgaacccctg caataactgt cacgcccccc tgcaataact gtcacgaacc cctgcaataa
3061 ctgtcacgcc cccaaacctg caaacccagc aggggcgggg gctggcgggg tgttggaaaa
3121 atccatccat gattatctaa gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg
3181 cgctgctgcc cttgcccaat atgcccggcc agaggccgga tagctggtct attcgctgcg
3241 ctaggctaca caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa
3301 accccacacc aaaccccgca gaaatacgct ggagcgcttt tagccgcttt agcggccttt
3361 ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct cggtcgatca
3421 ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa caaggcgcgg tcgtggtcgc
3481 gttcaaggta cgcatccatt gccgccatga gccgatcctc cggccactcg ctgctgttca
3541 ccttggccaa aatcatggcc cccaccagca ccttgcgcct tgtttcgttc ttgcgctctt
3601 gctgctgttc ccttgcccgc acccgctgaa tttcggcatt gattcgcgct cgttgttctt
3661 cgagcttggc cagccgatcc gccgccttgt tgctcccctt aaccatcttg acaccccatt
3721 gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg gcggcaatcc cgaccctact
3781 ttgtagggga gggcgcactt accggtttct cttcgagaaa ctggcctaac ggccaccctt
3841 cgggcggtgc gctctccgag ggccattgca tggagccgaa aagcaaaagc aacagcgagg
3901 cagcatggcg atttatcacc ttacggcgaa aaccggcagc aggtcgggcg gccaatcggc
3961 cagggccaag gccgactaca tccagcgcga aggcaagtat gcccgcgaca tggatgaagt
4021 cttgcacgcc gaatccgggc acatgccgga gttcgtcgag cggcccgccg actactggga
4081 tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc aaggaggtcg aatttgccct
4141 gccggtcgag ctgaccctcg accagcagaa ggcgctggcg tccgagttcg cccagcacct
4201 gaccggtgcc gagcgcctgc cgtatacgct ggccatccat gccggtggcg gcgagaaccc
4261 gcactgccac ctgatgatct ccgagcggat caatgacggc atcgagcggc ccgccgctca
4321 gtggttcaag cggtacaacg gcaagacccc ggagaagggc ggggcacaga agaccgaagc
4381 gctcaagccc aaggcatggc ttgagcagac ccgcgaggca tgggccgacc atgccaaccg
4441 ggcattagag cgggctggcc acgacgcccg cattgaccac agaacacttg aggcgcaggg
4501 catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg gtggagatgg aaggccgggg
4561 catccgcacc gaccgggcag acgtggccct gaacatcgac accgccaacg cccagatcat
4621 cgacttacag gaataccggg aggcaataga ccatgaacgc aatcgacaga gtgaagaaat
4681 ccagaggcat caacgagtta gcggagcaga tcgaaccgct ggcccagagc atggcgacac
4741 tggccgacga agcccggcag gtcatgagcc agacccagca ggccagcgag gcgcaggcgg
4801 cggagtggct gaaagcccag cgccagacag gggcggcatg ggtggagctg gccaaagagt
4861 tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag cgcccggagc gcgtcgcggg
4921 ggtggcactg gaagctatgg ctaaccgtga tgctggcttc catgatgcct acggtggtgc
4981 tgctgatcgc atcgttgctc ttgctcgacc tgacgccact gacaaccgag gacggctcga
5041 tctggctgcg cttggtggcc cgatgaagaa cgacaggact ttgcaggcca taggccgaca
5101 gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc agggacgcca ccaccggcca
5161 gatgatgaac cgggaatggt cagccgccga agtgctccag aacacgccat ggctcaagcg
5221 gatgaatgcc cagggcaatg acgtgtatat caggcccgcc gagcaggagc ggcatggtct
5281 ggtgctggtg gacgacctca gcgagtttga cctggatgac atgaaagccg agggccggga
5341 gcctgccctg gtagtggaaa ccagcccgaa gaactatcag gcatgggtca aggtggccga
5401 cgccgcaggc ggtgaacttc gggggcagat tgcccggacg ctggccagcg agtacgacgc
5461 cgacccggcc agcgccgaca gccgccacta tggccgcttg gcgggcttca ccaaccgcaa
5521 ggacaagcac accacccgcg ccggttatca gccgtgggtg ctgctgcgtg aatccaaggg
5581 caagaccgcc accgctggcc cggcgctggt gcagcaggct ggccagcaga tcgagcaggc
5641 ccagcggcag caggagaagg cccgcaggct ggccagcctc gaactgcccg agcggcagct
5701 tagccgccac cggcgcacgg cgctggacga gtaccgcagc gagatggccg ggctggtcaa
5761 gcgcttcggt gatgacctca gcaagtgcga ctttatcgcc gcgcagaagc tggccagccg
5821 gggccgcagt gccgaggaaa tcggcaaggc catggccgag gccagcccag cgctggcaga
5881 gcgcaagccc ggccacgaag cggattacat cgagcgcacc gtcagcaagg tcatgggtct
5941 gcccagcgtc cagcttgcgc gggccgagct ggcacgggca ccggcacccc gccagcgagg
6001 catggacagg ggcgggccag atttcagcat gtagtgcttg cgttggtact cacgcctgtt
6061 atactatgag tactcacgca cagaaggggg ttttatggaa tacgaaaaaa gcgcttcagg
6121 gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg cccggtggct ttggttatac
6181 gtcaaacaag gccgaggctg gccgcttttc agtcgctgat atggccagcc ttaaccttga
6241 cggctgcacc ttgtccttgt tccgcgaaga caagcctttc ggccccggca agtttctcgg
6301 tgactgatat gaaagaccaa aaggacaagc agaccggcga cctgctggcc agccctgacg
6361 ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa agggatgcgt cagcgcaagt
6421 tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg cctggaagaa ctcagagcgg
6481 cgcagggcgg gggtagtgac cccgccagcg cctaaccacc aactgcctgc aaaggaggca
6541 atcaatggct acccataagc ctatcaatat tctggaggcg ttcgcagcag cgccgccacc
6601 gctggactac gttttgccca acatggtggc cggtacggtc ggggcgctgg tgtcgcccgg
6661 tggtgccggt aaatccatgc tggccctgca actggccgca cagattgcag gcgggccgga
6721 tctgctggag gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg ccgaagaccc
6781 gcccaccgcc attcatcacc gcctgcacgc ccttggggcg cacctcagcg ccgaggaacg
6841 gcaagccgtg gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat
6901 ggccccggag tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct
6961 ggacacgctg cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt
7021 catcggtcgc atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca
7081 tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag
7141 ctcggtactg gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga
7201 ggccgaggaa tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag
7261 caaggccaac tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt
7321 gctcaagccc gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc
7381 ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg
7441 gcctgttccg tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg
7501 actacggcga cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc
7561 tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg
7621 aacccaagac cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg
7681 tcaccgctga tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg
7741 cagaggtcga tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga
7801 aggtatccat catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt
7861 acgccagcga cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg
7921 ccgtcatggg tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca
7981 gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg
8041 gcaaggcttc catagatacc ttgtgcggct atgtctggcc gtcagaggcc agtggttcga
8101 ccatgcgcaa gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc gcgctgggct
8161 ggacggtaac cgagttcgcg gcgggcaagt acgacatcac ccggcccaag gcggcaggct
8221 gacccccccc actctattgt aaacaagaca tttttatctt ttatattcaa tggcttattt
8281 tcctgctaat tggtaatacc atgaaaaata ccatgctcag aaaaggctta acaatatttt
8341 gaaaaattgc ctactgagcg ctgccgcaca gctccatagg ccgctttcct ggctttgctt
8401 ccagatgtat gctcttctgc tcctgcagtt cgggggcatg gatgcgcgga tagccgctgc
8461 tggtttcctg gatgccgacg gatttgcact gccggtagaa ctccgcgagg tcgtccagcc
8521 tcaggcagca gctgaaccaa ctcgcgaggg gatcgagccc ggggtgggcg aagaactcca
8581 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
8641 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
8701 cttggtcggt catttcgcct cgaggcgaac cccagagtcc cgctcagaag aactcgtcaa
8761 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga
8821 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt
8881 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat
8941 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt
9001 cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt
9061 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc
9121 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca
9181 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct
9241 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca
9301 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca
9361 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg
9421 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga
9481 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
9541 gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatccttg
9601 gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc
9661 cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg
9721 taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag
9781 cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt
9841 tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa gctagcttgg
9901 ctgcaggggg gggggggggg gttccattgt tcattccacg gacaaaaaca gagaaaggaa
9961 acgacagagg ccaaaaagct cgctttcagc acctgtcgtt tcctttcttt tcagagggta
10021 ttttaaataa aaacattaag ttatgacgaa gaagaacgga aacgccttaa accggaaaat
10081 tttcataaat agcgaaaacc cgcgaggtcg ccgccccgta acctgtcgga tcaccggaaa
10141 ggacccgtaa agtgataatg attatcatct acatatcaca acgtgcgtgg aggccatcaa
10201 accacgtcaa ataatcaatt atgacgcagg tatcgtatta attgatctgc atcaacttaa
10261 cgtaaaaaca acttcagaca atacaaatca gcgacactga atacggggca acctcatgtc
10321 c
//
