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pTK-Red-Firefly-Luc载体质粒基本信息
pTK-Red-Firefly-Luc质粒图谱载体图谱和pTK-Red-Firefly-Luc载体序列质粒序列多克隆位点信息
pTK-Red-Firefly-Luc质粒载体简介
pTK-Red-Firefly-Luc质粒序列载体序列
LOCUS Exported File 6718 bp ds-DNA circular SYN 26-七月-2013
DEFINITION Control vector for constitutive low-level expression of
intracellular red firefly luciferase under control of the HSV TK
promoter.
ACCESSION .
VERSION .
KEYWORDS pTK-Red Firefly Luc
SOURCE http://www.biofeng.com/
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6718)
AUTHORS Thermo Scientific (Pierce)
TITLE Direct Submission
JOURNAL Exported 2016-07-24 from SnapGene Viewer 1.5.3
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6718
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
promoter 26..777
/note="HSV TK promoter"
/note="herpes simplex virus thymidine kinase promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 790..2436
/codon_start=1
/product="intracellular red firefly luciferase"
/note="Red Firefly Luciferase"
/note="human codon-optimized"
/note="color: #ff0000"
/translation="MENMENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFT
NAVTGVDYSYAEYLEKSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGV
APTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGY
QCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFS
HARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQD
YKCTYVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQ
GYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPML
MKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELES
VLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGG
VRFVDEVPKGLTGKIDGRAIREILKKPVAKM"
polyA_signal 2462..2573
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
/note="color: #a6acb3"
promoter 2696..3025
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
/note="color: #ffffff; direction: RIGHT"
rep_origin 2876..3011
/note="SV40 ori"
/note="SV40 origin of replication"
/note="color: #ffff00"
promoter 3073..3120
/note="EM7 promoter"
/note="synthetic bacterial promoter?"
/note="color: #ffffff; direction: RIGHT"
CDS 3139..3738
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/note="color: #ccffcc"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 3868..3989
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
CDS complement(4042..4902)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
???1:?4042?..?4833?/?#ccffcc
???2:?4834?..?4902?/?#ccffcc?/?signal?sequence
Cleavage site after base 4833"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5166..5754
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
protein_bind 6496..6512
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/note="color: #31849b"
misc_feature 6627..6718
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human?alpha-2 globin gene"
/note="color: #a6acb3"
ORIGIN
1 actagtctcg aggaattcca agcttaatga gtcttcggac ctcgcggggg ccgcttaagc
61 ggtggttagg gtttgtctga cgcgggggga gggggaagga acgaaacact ctcattcgga
121 ggcggctcgg ggtttggtct tggtggccac gggcacgcag aagagcgccg cgatcctctt
181 aagcaccccc ccgccctccg tggaggcggg ggtttggtcg gcgggtggta actggcgggc
241 cgctgactcg ggcgggtcgc gcgccccaga gtgtgacctt ttcggtctgc tcgcagaccc
301 ccgggcggcg ccgccgcggc ggcgacgggc tcgctgggtc ctaggctcca tggggaccgt
361 atacgtggac aggctctgga gcatccgcac gactgcggtg atattaccgg agaccttctg
421 cgggacgagc cgggtcacgc ggctgacgcg gagcgtccgt tgggcgacaa acaccaggac
481 ggggcacagg tacactatct tgtcacccgg aggcgcgagg gactgcagga gcttcaggga
541 gtggcgcagc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
601 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
661 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca
721 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaagga
781 tccgccacca tggaaaatat ggaaaacgac gagaacatcg tggtgggccc caagcccttc
841 taccccatcg aggaaggcag cgccggcacc cagctgcgga agtacatgga aagatacgcc
901 aagctgggcg ccattgcctt caccaacgcc gtgaccggcg tggactacag ctacgccgag
961 tacctggaaa agagctgctg cctgggcaag gctctgcaga actacggcct ggtggtggac
1021 ggccggatcg ccctgtgcag cgagaactgc gaggaattct tcatccccgt gatcgccggc
1081 ctgttcatcg gcgtgggcgt ggctcccacc aacgagatct acaccctgcg ggagctggtg
1141 cacagcctgg gcatcagcaa gcccaccatc gtgttcagca gcaagaaggg cctggacaaa
1201 gtcatcaccg tgcagaaaac cgtgaccacc atcaagacca tcgtgatcct ggacagcaag
1261 gtggactacc ggggctacca gtgcctggac accttcatca agcggaacac cccccctggc
1321 ttccaggcca gcagcttcaa gaccgtggag gtggaccgga aagaacaggt ggccctgatc
1381 atgaacagca gcggcagcac cggcctgccc aagggcgtgc agctgaccca cgagaacacc
1441 gtgacccggt tcagccacgc cagggacccc atctacggca accaggtgtc ccccggcacc
1501 gccgtgctga ccgtggtgcc cttccaccac ggcttcggca tgttcaccac cctgggctac
1561 ctgatctgcg gcttccgggt ggtgatgctg accaagttcg acgaggaaac cttcctgaaa
1621 accctgcagg actacaagtg cacctacgtg attctggtgc ccaccctgtt cgccatcctg
1681 aacaagagcg agctgctgaa caagtacgac ctgagcaacc tggtggagat cgccagcggc
1741 ggagcccccc tgagcaaaga agtgggagag gccgtcgcca ggcggttcaa tctgcccggc
1801 gtgcggcagg gctacggcct gaccgagaca accagcgcca tcatcatcac ccccgagggc
1861 gacgacaagc ctggagccag cggcaaggtg gtgcccctgt tcaaggccaa agtgatcgac
1921 ctggacacca agaagagcct gggccccaac agacggggcg aagtgtgcgt gaagggcccc
1981 atgctgatga agggctacgt gaacaacccc gaggccacca aagagctgat cgacgaagag
2041 ggctggctgc acaccggcga catcggctac tacgacgaag agaagcactt cttcatcgtg
2101 gaccggctga agagcctgat caagtacaag ggctatcagg tgccccctgc cgagctggaa
2161 agcgtcctgc tgcagcaccc cagcatcttc gacgccggcg tggccggggt gccagatcct
2221 gtggccggcg agctgcctgg cgccgtggtg gtgctggaat ccggcaagaa catgaccgag
2281 aaagaagtga tggactacgt cgccagccag gtgtccaacg ccaagcggct gagaggcggc
2341 gtgagattcg tggacgaagt gccaaagggc ctgaccggca agatcgacgg cagggccatc
2401 cgggagatcc tgaagaaacc cgtggccaag atgtgatgag cggccgcaaa atcagcctcg
2461 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
2521 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcacaacac
2581 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
2641 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
2701 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
2761 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
2821 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
2881 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
2941 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
3001 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
3061 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
3121 aaggtgagga actaaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
3181 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
3241 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
3301 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
3361 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
3421 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
3481 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
3541 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
3601 ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc tccccttcta cgagcggctc
3661 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
3721 cgcaagcccg gtgcctgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
3781 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
3841 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
3901 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
3961 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
4021 agcttggcgt aatcatggtc attaccaatg cttaatcagt gaggcaccta tctcagcgat
4081 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
4141 ggagggctta ccatctggcc ccagcgctgc gatgataccg cgagaaccac gctcaccggc
4201 tccggattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
4261 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
4321 gccagttaat agtttgcgca acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc
4381 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
4441 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
4501 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
4561 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
4621 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
4681 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
4741 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
4801 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
4861 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atattcttcc tttttcaata
4921 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
4981 gaaaaataaa caaatagggg tcagtgttac aaccaattaa ccaattctga acattatcgc
5041 gagcccattt atacctgaat atggctcata acaccccttg ctcatgacca aaatccctta
5101 acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
5161 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
5221 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
5281 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttagcccac
5341 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
5401 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
5461 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
5521 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
5581 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
5641 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
5701 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
5761 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
5821 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
5881 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaggcgag
5941 agtagggaac tgccaggcat caaactaagc agaaggcccc tgacggatgg cctttttgcg
6001 tttctacaaa ctctttctgt gttgtaaaac gacggccagt cttaagctcg ggccccctgg
6061 gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg cttcggcggg
6121 tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag ggcgcctgtc
6181 actttgcttg atatatgaga attatttaac cttataaatg agaaaaaagc aacgcacttt
6241 aaataagata cgttgctttt tcgattgatg aacacctata attaaactat tcatctatta
6301 tttatgattt tttgtatata caatatttct agtttgttaa agagaattaa gaaaataaat
6361 ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa attatacatg tcaacgataa
6421 tacaaaatat aatacaaact ataagatgtt atcagtattt attatcattt agaataaatt
6481 ttgtgtcgcc cttaattgtg agcggataac aattacgagc ttcatgcaca gtggcgttga
6541 cattgattat tgactagcat gttctttcct gcgttatccc ctgattctgt ggataaccgt
6601 attaccgcca tgcattagtt attaataaca tacgctctcc atcaaaacaa aacgaaacaa
6661 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctct
//
