| 出品公司: | INVITROGEN |
|---|---|
| 载体名称: | pCMV-Gaussia-Dura-Luc ; pCMV-Gaussia-Dura Luc |
| 质粒类型: | 哺乳动物载体；分泌型荧光素酶报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 5482 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | Gaussia-Dura 分泌型荧光素酶 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | 常规哺乳动物细胞 |
| 备注: | pCMV-Gaussia-Dura-Luc载体表达分泌型的荧光素酶，可直接在培养基中进行检测。 |
| 产品目录号: | 16191 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCMV-Gaussia-Dura-Luc载体质粒基本信息
pCMV-Gaussia-Dura-Luc质粒图谱载体图谱和pCMV-Gaussia-Dura-Luc载体序列质粒序列多克隆位点信息
pCMV-Gaussia-Dura-Luc质粒载体简介
pCMV-Gaussia-Dura Luc 载体简介
The Thermo Scientific pCMV-Gaussia-Dura Luc vector is a derivative of pMCS-Gaussia-Dura Luc. It contains a mutant form of the Gaussia luciferase gene (conferring better bioluminescent signal stability than the native luciferase) under the control of the strong Cytomegalovirus (CMV) promoter. This constitutive expression vector can be used as a normalization control to account for experimental variation in combination with other reporters.
载体特征:
Strong (CMV) constitutive promoter for co-transfection and normalization
Naturally-secreting Gaussia-Dura luciferase gene, optimized for high expression and glow-stability in mammalian systems
Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
High-copy pUC bacterial DNA replication origin
Gaussia-Dura luciferase (approx. 20kDa) is a secreted protein that enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The parent pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
pCMV-Gaussia-Dura-Luc质粒序列载体序列
LOCUS Exported 5482 bp ds-DNA circular SYN 24-JUL-2016
DEFINITION Control vector for constitutive high-level expression of secreted
Gaussia-Dura luciferase under control of the CMV promoter.
ACCESSION .
VERSION .
KEYWORDS pCMV-Gaussia-Dura Luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5482)
AUTHORS Thermo Scientific (Pierce)
TITLE Direct Submission
JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5482
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 63..366
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 367..570
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 615..633
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 646..1203
/codon_start=1
/product="enhanced variant of secreted Gaussia luciferase"
/note="Gaussia-Dura Luc"
/note="human codon-optimized"
/translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
KLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
QGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
RCATFASKIQGQVDKIKGAGGD"
polyA_signal 1226..1337
/note="BGH pA"
/note="bovine growth hormone polyadenylation signal"
promoter 1460..1789
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1640..1775
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 1837..1884
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 1903..2502
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="Puromycin"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 2632..2753
/note="SV40 pA"
/note="SV40 polyadenylation signal"
CDS complement(2806..3666)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="Ampicillin"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3930..4518
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5260..5276
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature 5391..5482
/note="TPS"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
541 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
601 tggcttatcg aaattaatac gactcactat aggggatccg ccaccatggg agtcaaagtt
661 ctgtttgccc tgatctgcat cgctgtggcc gaggccaagc ccaccgagaa caacgaagac
721 ttcaacatcg tggccgtggc cagcaacttc gcgaccacgg atctcgatgc tgaccgcggg
781 aagttgcccg gcaagaagct gccgctggag gtgctcaaag agttggaagc caatgcccgg
841 aaagctggct gcaccagggg ctgtctgatc tgcctgtccc acatcaagtg cacgcccaag
901 atgaagaagt tcatcccagg acgctgccac acctacgaag gcgacaaaga gtccgcacag
961 ggcggcatag gcgaggcgat cgtcgacatt cctgagattc ctgggttcaa ggacttggag
1021 cccttggagc agttcatcgc acaggtcgat ctgtgtgtgg actgcacaac tggctgcctc
1081 aaagggcttg ccaacgtgca gtgttctgac ctgctcaaga agtggctgcc gcaacgctgt
1141 gcgacctttg ccagcaagat ccagggccag gtggacaaga tcaagggggc cggtggtgac
1201 taagcggccg caaaatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
1261 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
1321 aatgaggaaa ttgcatcaca acactcaacc ctatctcggt ctattctttt gatttataag
1381 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
1441 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
1501 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
1561 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
1621 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
1681 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
1741 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
1801 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
1861 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatgaccga gtacaagccc
1921 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
1981 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
2041 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
2101 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
2161 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
2221 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
2281 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
2341 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacatc cgcgccccgc
2401 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
2461 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacacgtgct acgagatttc
2521 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
2581 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
2641 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
2701 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
2761 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcattacc aatgcttaat
2821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
2881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagcg ctgcgatgat
2941 accgcgagaa ccacgctcac cggctccgga tttatcagca ataaaccagc cagccggaag
3001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
3061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccatcgc
3121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
3181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
3241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
3301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
3361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
3421 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
3481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
3541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
3601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3661 actcatattc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggtcagtg ttacaaccaa
3781 ttaaccaatt ctgaacatta tcgcgagccc atttatacct gaatatggct cataacaccc
3841 cttgctcatg accaaaatcc cttaacgtga gttacgcgcg cgtcgttcca ctgagcgtca
3901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
3961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
4021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
4081 ctagtgtagc cgtagttagc ccaccacttc aagaactctg tagcaccgcc tacatacctc
4141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
4201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
4261 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
4321 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
4381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
4441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
4501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
4561 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
4621 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
4681 gtgagcgagg aagcggaagg cgagagtagg gaactgccag gcatcaaact aagcagaagg
4741 cccctgacgg atggcctttt tgcgtttcta caaactcttt ctgtgttgta aaacgacggc
4801 cagtcttaag ctcgggcccc ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
4861 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
4921 tcagaatatt taagggcgcc tgtcactttg cttgatatat gagaattatt taaccttata
4981 aatgagaaaa aagcaacgca ctttaaataa gatacgttgc tttttcgatt gatgaacacc
5041 tataattaaa ctattcatct attatttatg attttttgta tatacaatat ttctagtttg
5101 ttaaagagaa ttaagaaaat aaatctcgaa aataataaag ggaaaatcag tttttgatat
5161 caaaattata catgtcaacg ataatacaaa atataataca aactataaga tgttatcagt
5221 atttattatc atttagaata aattttgtgt cgcccttaat tgtgagcgga taacaattac
5281 gagcttcatg cacagtggcg ttgacattga ttattgacta gcatgttctt tcctgcgtta
5341 tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat aacatacgct
5401 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
5461 gcaggtgcca gaacatttct ct
//
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