| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 别名: | pET22b, pet 22b, pET-22b(+) |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5500bp |
| 5' 测序引物及序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
| 3' 测序引物序列: | T7t: 5'-GCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-pelB; C-His |
| 载体抗性: | 氨苄 |
| 备注: |
pET22b载体含有PelB信号肽序列， 能够将表达的目的蛋白定位在细胞外周质腔。
|
| 产品目录号: | 69744-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-22b(+)载体 pet22b质粒基本信息
pET-22b(+)质粒图谱 pet22b载体图谱和pET-22b(+)载体序列 pet22b质粒序列多克隆位点信息
pET-22b(+)质粒 pet22b载体简介
pET-22b(+)载体(货号：69744-3)携带有一个N端的pelB信号肽序列，能够将表达的目的蛋白定位于外周质腔，同时载体含有C端His标签。载体的单一的多克隆位点见上面的环状质粒图谱。注意：载体序列是以pBR322质粒的编码规矩进行编码的，所以T7蛋白表达区在质粒图谱上面是反向的。 T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒 粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。
pET-22b(+)质粒序列 pet22b载体序列
LOCUS pET-22b(+) 5493 bp DNA circular 11-SEP-2008 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT LSOWNER| COMMENT VNTNAME|pET-22b(+)| COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTOAUTHORAD1|Novagen Inc.| FEATURES Location/Qualifiers promoter complement(361..377) /vntifkey="30" /label=T7\promoter misc_signal complement(224..289) /vntifkey="87" /label=pelB\sequence protein_bind complement(140..157) /vntifkey="31" /label=His\tag terminator complement(26..72) /vntifkey="43" /label=T7\terminator CDS 764..1843 /vntifkey="4" /label=lac\I rep_origin complement(3277..3277) /vntifkey="33" /label=ColE1\pBR322\origin CDS complement(4038..4895) /vntifkey="4" /label=bla\(Ap)\sequence rep_origin complement(5027..5482) /vntifkey="33" /label=f1\origin CDS complement(334..358) /vntifkey="4" /label=lac\operator BASE COUNT 1302 a 1456 c 1461 g 1274 t ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg 241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc 301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc 361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc 421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc 481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg 541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt 601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg 661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat 721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt 781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca 841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa 901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt 961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg 1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc 1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta 1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt 1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg 1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc 1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac 1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt 1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa 1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc 1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt 1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca 1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag 1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca 1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg 1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg 2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct 2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc 2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga 2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg 2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg 2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta 2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat 2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg 2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta 2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt 2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca 2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag 2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt 2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac 3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 3961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 4021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 4081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 4141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 4201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 4261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 4321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 4381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 4441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 4501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 4561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 4621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 4681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 4741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 4801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 4861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 4921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 4981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt 5041 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 5101 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 5161 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 5221 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 5281 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 5341 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 5401 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 5461 aatgcgccgc tacagggcgc gtcccattcg cca //
pET-22b(+)载体图谱 pet22b质粒图谱pdf版和pET-22b(+)载体序列 pet22b质粒序列等相关资料下载
pET-22b(+)质粒 pet22b载体应用举例
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