pIREShyg3质粒图谱、载体图谱、序列、价格、抗性、测序引物、大小等信息

出品公司:	Clontech
载体名称:	pIREShyg3
质粒类型:	哺乳动物细胞表达载体
启动子:	CMV
表达水平:	高
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5752 bp
5' 测序引物:	CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3'
3' 测序引物:	pIRES-R:5'-GCCCTAGATGCATGCTCG-3'
载体标签:	无
载体抗性:	氨苄青霉素（Ampicillin）
筛选标记:	潮霉素（Hygromycin）
备注:	pIREShyg3载体含有IRES元件，目的基因与抗性基因以双顺反子的形式共转录。
产品目录号:	631620
稳定性:	稳表达或瞬表达
组成型:	组成型
病毒/非病毒:	非病毒

Description
pIREShyg3 contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV), which permits the translation of two open reading frames from one messenger RNA (1–3). After selection with hygromycin B, nearly all surviving colonies will stably express the gene of interest, thus decreasing the need to screen large numbers of colonies to find functional clones. To select for cells that express high levels of the gene of interest, the selective pressure for antibiotic resistance was increased by shifting the hygromycin B phosphotransferase gene downstream to a less optimal position for translation as directed by the IRES sequence (3). By decreasing the level of expression of the antibiotic resistance marker, the selective pressure on the entire expression cassette is increased, resulting in selection for cells that express the entire transcript, including the gene of interest, at high levels. The expression cassette of pIREShyg3 contains the human cytomegalovirus (CMV) major immediate early promoter/enhancer followed by a multiple cloning site (MCS) that precedes stop codons in all three reading frames, a synthetic intron known to enhance the stability of the mRNA (4), the ECMV IRES followed by the hygromycin B phosphotransferase gene, and the polyadenylation signal from SV40. Ribosomes can enter the bicistronic mRNA at the 5' end to translate the gene of interest and at the ECMV IRES to translate the antibiotic resistance marker.

Use
When using the pIREShyg3 Vector, the antibiotic exerts selective pressure on the entire expression cassette; thus, a high dose of antibiotic will select for cells expressing a high level of the gene of interest. This selective pressure also ensures that the expression of the gene of interest will be stable over time in culture. Unless your expression experiments require a pure population of cells, you can use the pool of cells surviving selection instead of isolating and characterizing clonal cell lines. We recommend selecting mammalian cultures in 250–600 μg/ml Hygromycin B (Cat. No. 631309) depending on the cell line. Be sure to establish a kill curve for each cell line and each lot of Hygromycin B to determine optimal selection concentration.

LOCUS       pIREShyg3   5752 bp    DNA circular    SYN
DEFINITION pIREShyg3
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="pIREShyg3"
                     /mol_type="other DNA"
     promoter        236..852
                     /label="CMV_immearly_promoter"
     misc_feature    315..602
                     /label="CAG_enhancer"
     misc_feature    769..789
                     /label="CMV_fwd_primer"
     promoter        863..881
                     /label="T7_promoter"
     misc_feature    1002..1297
                     /label="synth_int"
     misc_feature    1334..1898
                     /label="IRES"
     CDS             1819..2967
                     /label="ORF frame 1"
                     /translation="MLYMCLVEVKKTSRPPEPRGRGFPLKNTMISLPQPVPKMDRSGK
                     PELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDR
                     YVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAM
                     DAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQA
                     LDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFW
                     RPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRC
                     DAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
     CDS             complement(1834..2700)
                     /label="ORF frame 2"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP
                     WFGGPRRFFNLD*"
     gene            1933..2964
                     /label="hygroB"
                     /gene="hygroB"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP
                     WFGGPRRFFNLD*"
     terminator      3266..3494
                     /label="SV40_PA_terminator"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP
                     WFGGPRRFFNLD*"
     misc_feature    3464..3483
                     /label="EBV_rev_primer"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP
                     WFGGPRRFFNLD*"
     misc_feature    3516..3535
                     /label="EBV_rev_primer"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP
                     WFGGPRRFFNLD*"
     promoter        complement(3591..3609)
                     /label="M13_reverse_primer"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP
                     WFGGPRRFFNLD*"
     misc_feature    complement(3608..3630)
                     /label="M13_pUC_rev_primer"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP
                     WFGGPRRFFNLD*"
     promoter        complement(3644..3673)
                     /label="lac_promoter"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP
                     WFGGPRRFFNLD*"
     rep_origin      complement(3982..4601)
                     /label="pBR322_origin"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP
                     WFGGPRRFFNLD*"
     gene            complement(4756..5616)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLSGSIHLWYGLWQAYHRVFQRKTTSP
                     WFGGPRRFFNLD*"
     CDS             complement(4756..5616)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5658..5686)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG
   61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG
121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC
181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT
241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA
301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC
361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC
421 ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT
481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT
541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA
601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG
661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC
721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG
781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA
841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC
901 GAGCTCGGAT CTGTACAGGC CTTAAGCCGG CGCGCTAGCG CTTCGAAGGC GCCCGGGATC
961 CGATATCGTA CGTTAACATA GATAACTGAT CCAGTGTGCT GGAATTAATT CGCTGTCTGC
1021 GAGGGCCAGC TGTTGGGGTG AGTACTCCCT CTCAAAAGCG GGCATGACTT CTGCGCTAAG
1081 ATTGTCAGTT TCCAAAAACG AGGAGGATTT GATATTCACC TGGCCCGCGG TGATGCCTTT
1141 GAGGGTGGCC GCGTCCATCT GGTCAGAAAA GACAATCTTT TTGTTGTCAA GCTTGAGGTG
1201 TGGCAGGCTT GAGATCTGGC CATACACTTG AGTGACAATG ACATCCACTT TGCCTTTCTC
1261 TCCACAGGTG TCCACTCCCA GGTCCAACTG CAGGTCGAGC ATGCATCTAG GGCGGCCGCA
1321 CTAGAGGAAT TCCGCCCCTC TCCCTCCCCC CCCCCTAACG TTACTGGCCG AAGCCGCTTG
1381 GAATAAGGCC GGTGTGTGTT TGTCTATATG TTATTTTCCA CCATATTGCC GTCTTTTGGC
1441 AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG GGGTCTTTCC
1501 CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT TCCTCTGGAA
1561 GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA CCCCCCACCT
1621 GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC AAAGGCGGCA
1681 CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG GCTCTCCTCA
1741 AGCGTAGTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT GGGAATCTGA
1801 TCTGGGGCCT CGGTGCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA AACGTCTAGG
1861 CCCCCCGAAC CACGGGGACG TGGTTTTCCT TTGAAAAACA CGATGATAAG CTTGCCACAA
1921 CCCGTACCAA AGATGGATAG ATCCGGAAAG CCTGAACTCA CCGCGACGTC TGTCGAGAAG
1981 TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGGA GGGCGAAGAA
2041 TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT AAATAGCTGC
2101 GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC CGCGCTCCCG
2161 ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC TGACCTATTG CATCTCCCGC
2221 CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC TGTTCTGCAG
2281 CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC TTAGCCAGAC GAGCGGGTTC
2341 GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT CATATGCGCG
2401 ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT CAGTGCGTCC
2461 GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG ACTGCCCCGA AGTCCGGCAC
2521 CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG ACAATGGCCG CATAACAGCG
2581 GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT ACGAGGTCGC CAACATCTTC
2641 TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA GCGGAGGCAT
2701 CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC TCCGCATTGG TCTTGACCAA
2761 CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG CTTGGGCGCA GGGTCGATGC
2821 GACGCAATCG TCCGATCCGG AGCCGGGACT GTCGGGCGTA CACAAATCGC CCGCAGAAGC
2881 GCGGCCGTCT GGACCGATGG CTGTGTAGAA GTACTCGCCG ATAGTGGAAA CCGACGCCCC
2941 AGCACTCGTC CGAGGGCAAA GGAATAGAGT AGATGCCGAC CGAACAAGAG CTGATTTCGA
3001 GAACGCCTCA GCCAGCAACT CGCGCGAGCC TAGCAAGGCA AATGCGAGAG AACGGCCTTA
3061 CGCTTGGTGG CACAGTTCTC GTCCACAGTT CGCTAAGCTC GCTCGGCTGG GTCGCGGGAG
3121 GGCCGGTCGC AGTGATTCAG GCCCTTCTGG ATTGTGTTGG TCCCCAGGGC ACGATTGTCA
3181 TGCCCACGCA CTCGGGTGAT CTGACTGATC CCGCAGATTG GAGATCGCCG CCCGTGCCTG
3241 CCGATTGGGT GCAGATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT
3301 TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA
3361 ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC
3421 ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC
3481 ATCAATGTAT CTTAACGCTC GAGTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
3541 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA
3601 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG
3661 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG
3721 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA
3781 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
3841 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
3901 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
3961 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
4021 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
4081 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
4141 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG
4201 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
4261 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
4321 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
4381 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC
4441 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
4501 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
4561 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
4621 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
4681 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
4741 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
4801 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
4861 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
4921 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
4981 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
5041 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
5101 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
5161 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
5221 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
5281 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
5341 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
5401 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
5461 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
5521 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
5581 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
5641 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
5701 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TC

//

pHY15	pEF6/myc-His B
pCREj-2	pET-21a(+)
pPIC9k-His	pALEX a,b,c
pCGN978	pCDH-CMV-MCS-EF1a-RFP+BSD
pAcGFP1-N2	pCH2
pLexm	pBAD18
pMal-c4X	pHS85
pUCTag	pFA1
pDsRed-Monomer	pLVX-DD-ZsGreen1 Control
PX543	pTi
pSUMO	pCYM1
c-Flag pcDNA3	pAN7-1
pET-28a-BFP-C	pGL4.41[luc2P/HSE/Hygro]
pL-brevisGH3-MBP	pBD-NF-kB
pUG6	pIJ702-del35-55
pFLAG-CMV2	pET-32 EK/LIC
pEF1/V5-HisA	pSH65
pAD-SV40T	pOrf681_TA
pET-41a(+)	pSET152
pGL4.38[luc2P/p53 RE/Hygro]	pCL-Ampho
pRSET-EGFP	pcDNA4/HisMax B
pMT-Bip-V5-HisA	pCRE-DD-AmCyan1
pNEO-ars	pSP72
pCRE-MetLuc2-Reporter	pDEST26
pREP4	pBT-4
pSUPER.neo+gfp	pET-12c(+)
pAGO	pOM2
pDC515	pATPase_TA
pTP-4	pUC4-KISS
pcDNA5/FRT	pKLAC1
pPIC ZαGB	pML21
pBluescript II KS(-)	pcDNA5/FRT/V5-His-TOPO
ISceI-GR-RFP	pSC101
Pgex-HRV3C	pMTD1.3xA
pPIC9	pCas9D10A-GFP
EWD299	pJSC73
pSC101-EcoRImeth+	pHB-1
pHV23	pWF1
pBad43	pFLAG-CMV-3
pMDLg/pRRE	pMal-c2G
pBBR1MCS-2	pNEO
pTet-Splice	pACT-MyoD
pIC20H	pRB373
pcDNA3.1(+)/CAT	pGL4.45[luc2P/ISRE/Hygro]
pENTR11-Dual	pLLP-OmpA
pIκB-EGFP	pcDNA6.2-DsRed2-MCS1 miR
pcDNA3.1-GP130v1	pVPack-GP
pHSG422	pdKeima-Red-S1
pGL4.72[hRlucCP]	pLNCX2
pET-32a(+)	pProLabel-C

pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-	pICH86966::AtU6p::sgRNA_PDS
pAAVS1-PDi-CRISPRn	pPPEM
pAAV-EFS-CjCas9-eGFP-HIF1a	LB1366 (Bcl-2 promoter with MHS1234)
pGH335_MS2-AID*Δ-Hygro	pAAV2/8
pDIRECT_23D	pNEU-hMbPylRS-4xU6M15
MSCV-(pBabe mcs)- human p210BCR-ABL-IRES	TAL3393
pHAGE-PTCH1	pGL3 2 kb prom. CD274
AbVec1.1-IGKC	pNeae2
pNN9	pYES-mtGFP
800 pSG5L HA PTEN wt	pT3-Neo-EF1a-GFP
BECLIN-HA WT	pAC1404-pCR8-mRuby2_NLSPUFa
pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I	pAc-Neo-OVA
p201G Cas9	ilux pGEX(-)
Cbh_v5 AAV-CBE N-terminal	pEGFP-LC3
pTEF:ATP	pCMV-MMLVgag-3xNES-ABE8e
pLentiV2-dCas9-VP64	pLVX-M-puro
PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a	pLJC5-3XHA-EGFP-PEX26
pLenti-EML4-ALK variant 3a	pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV
pCW-Cas9	pSELECT-HA-mFOXO1
pMSCV-hygro-STING	3XMEF2-luc
p58sheLL	phage UbiC NLS HA stdMCP stdHalo
pAAVS1-PC-GCaMP6f	pBHt2
pSBtet-GP	pM-Cas9
pLenti X2 Puro DEST (w16-1)	pMal-T-Avi-His/BirA
EC2_10_dCas9_VPR_HC_sgRNA	pXAT2
pC003 - LshC2C2 locus into pACYC184 for	pMXs-hOCT4 (Plath)
pCMV-hA3G-BE3	NM9-SpCas9-NLS3
pAraGFPCDF	pET28a-At-TRL
pMSCV-IRES-GFP II (pMIG II)	pFRT/TO/GFP-SCAF4 (CRISPR_resistant)
pM1s3AsR_TS-C7	pCAG-GBP1-10gly-lexADBD
CKS2 gRNA (BRDN0001149143)	EC2_3_dCas9_Mxi1_sgRNA
EC1000	Lenti-luciferase-P2A-Neo
LeGO-G	c-myc-PT3EF1a
pTRKH2	FUmGW
pRC-CMV-Rev1b	pCS2TAL3-RR
pFC334	pCS2TAL3-DD
pTRE Tight ArchT-GFP	pGGA008
pCDH-puro-Bcl-XL	pEGFP-puro
pQUAST>stop>mCD8-GFP	pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK
pSR47	Tet-O-FUW-Neurod1
PB_tre_Cas9	pJSC131 - Bacterial expression plasmid f
BPK3082	VVT1
pJKR-L-tetR	pMonAID_nCas9-PmCDA_Hyg_ALS
pNK9	pAAV-EF1a-FLEX-GTB
pUCC001	401 pSG5L
1306 pSG5L HA FKHR wt	GST-HA-GFP11-N1
pRAD1	pCAS9-mCherry empty
pCXLE-hOCT3/4	pcDNA3.4_7C11-1 heavy chain
pAc-sgRNA-Cas9	pLenti-DsRed_IRES_MAPT:EGFP
pT4-CMV-GFP	pMJ329: pCMV-MmeFz2

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