pHT-XCR6

价格:2800元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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pHT-XCR6载体质粒基本信息

出品公司: Molecularcloud
载体名称: pHT-XCR6
质粒类型: 枯草芽孢杆菌表达载体,穿梭载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 14 kb
5’测序引物: --
3’测序引物: --
载体抗性: 氨苄青霉素
克隆菌株: DH5 alpha
宿主细胞(系): 枯草芽孢杆菌
产品目录号:  MC_0068418
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒
备注: The plasmid is used for the expression of Cpf1 and NgAgo in B. subtilis,and can replicate in E. coli.
参考文献: Wu, Y., Liu, Y., Lv, X., Li, J., Du, G., & Liu, L. (2020). CAMERS‐B: CRISPR/Cpf1 assisted multiple‐genes editing and regulation system for Bacillus subtilis. Biotechnology and Bioengineering.

pHT-XCR6质粒图谱载体图谱和pHT-XCR6载体序列质粒序列多克隆位点信息

pHT-XCR6载体图谱

pHT-XCR6质粒载体简介

pHT-XCR6质粒序列载体序列

LOCUS       pHT-XCR6               14002 bp DNA     circular SYN 12-NOV-2024
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14002)
  AUTHORS   Ming
  TITLE     Direct Submission
  JOURNAL   Exported Nov 12, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
            https://www.biofeng.com
COMMENT     VNTDATE|1073779200|
COMMENT     VNTDBDATE|1073779200|
COMMENT     VNTNAME|pHT-XCR6|
COMMENT     VNTAUTHORNAME|Ming|
FEATURES             Location/Qualifiers
     promoter        2151..2255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2256..3116
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      3287..3875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5279..6361)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    12161..12185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgagtgaag taaacctaaa aggaaataca gatgaattag tgtattatcg acagcaaacc
       61 actggaaata aaatcgccag gaagagaatc aaaaaaggga aagaagaagt ttattatgtt
      121 gctgaaacgg aagagaagat atggacagaa gagcaaataa aaaacttttc tttagacaaa
      181 tttggtacgc atatacctta catagaaggt cattatacaa tcttaaataa ttacttcttt
      241 gatttttggg gctatttttt aggtgctgaa ggaattgcgc tctatgctca cctaactcgt
      301 tatgcatacg gcagcaaaga cttttgcttt cctagtctac aaacaatcgc taaaaaaatg
      361 gacaagactc ctgttacagt tagaggctac ttgaaactgc ttgaaaggta cggttttatt
      421 tggaaggtaa acgtccgtaa taaaaccaag gataacacag aggaatcccc gatttttaag
      481 attagacgta aggttccttt gctttcagaa gaacttttaa atggaaaccc taatattgaa
      541 attccagatg acgaggaagc acatgtaaag aaggctttaa aaaaggaaaa agagggtctt
      601 ccaaaggttt tgaaaaaaga gcacgatgaa tttgttaaaa aaatgatgga tgagtcagaa
      661 acaattaata ttccagaggc cttacaatat gacacaatgt atgaagatat actcagtaaa
      721 ggagaaattc gaaaagaaat caaaaaacaa atacctaatc ctacaacatc ttttgagagt
      781 atatcaatga caactgaaga ggaaaaagtc gacagtactt taaaaagcga aatgcaaaat
      841 cgtgtctcta agccttcttt tgatacctgg tttaaaaaca ctaagatcaa aattgaaaat
      901 aaaaattgtt tattacttgt accgagtgaa tttgcatttg aatggattaa gaaaagatat
      961 ttagaaacaa ttaaaacagt ccttgaagaa gctggatatg ttttcgaaaa aatcgaacta
     1021 agaaaagtgc aataaactgc tgaagtattt cagcagtttt ttttatttag aaatagtgaa
     1081 aaaaatataa tcagggaggt atcaatattt aatgagtact gatttaaatt tatttagact
     1141 ggaattaata attaacacgt agactaatta aaatttaatg agggataaag aggatacaaa
     1201 aatattaatt tcaatcccta ttaaatttta acaagggggg gattaaaatt taattagagg
     1261 tttatccaca agaaaagacc ctaataaaat ttttactagg gttataacac tgattaattt
     1321 cttaatgggg gagggattaa aatttaatga caaagaaaac aatcttttaa gaaaagcttt
     1381 taaaagataa taataaaaag agctttgcga ttaagcaaaa ctctttactt tttcattgac
     1441 attatcaaat tcatcgattt caaattgttg ttgtatcata aagttaattc tgttttgcac
     1501 aaccttttca ggaatataaa acacatctga ggcttgtttt ataaactcag ggtcgctaaa
     1561 gtcaatgtaa cgtagcatat gatatggtat agcttccacc caagttagcc tttctgcttc
     1621 ttctgaatgt ttttcatata cttccatggg tatctctaaa tgattttcct catgtagcaa
     1681 ggtatgagca aaaagtttat ggaattgata gttcctctct ttttcttcaa cttttttatc
     1741 taaaacaaac actttaacat ctgagtcaat gtaagcataa gatgtttttc cagtcataat
     1801 ttcaatccca aatcttttag acagaaattc tggacgtaaa tcttttggtg aaagaatttt
     1861 tttatgtagc aatatatccg atacagcacc ttctaaaagc gttggtgaat agggcatttt
     1921 acctatctcc tctcattttg tggaataaaa atagtcatat tcgtccatct acctatccta
     1981 ttatcgaaca gttgaacttt ttaatcaagg atcagtcctt tttttcatta ttcttaaact
     2041 gtgctcttaa ctttaacaac tcgatttgtt tttccagatc tcgagggtaa ctagcctcgc
     2101 cgatcccgca agaggcccgg cagtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     2821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3421 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3901 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3961 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4021 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc atgcttaagt
     4081 tattggtatg actggtttta agcgcaaaaa aagttgcttt ttcgtaccta ttaatgtatc
     4141 gttagaaaac cgactgtaaa aagtacagtc ggcattatct catattataa aagccagtca
     4201 ttaggcctat ctgacaattc ctgaatagag ttcataaaca atcctgcatg ataaccatca
     4261 caaacagaat gatgtacctg taaagatagc ggtaaatata ttgaattacc tttattaatg
     4321 aattttcctg ctgtaataat gggtagaagg taattactat tattattgat atttaagtta
     4381 aacccagtaa atgaagtcca tggaataata gaaagagaaa aagcattttc aggtataggt
     4441 gttttgggaa acaatttccc cgaaccatta tatttctcta catcagaaag gtataaatca
     4501 taaaactctt tgaagtcatt ctttacagga gtccaaatac cagagaatgt tttagataca
     4561 ccatcaaaaa ttgtataaag tggctctaac ttatcccaat aacctaactc tccgtcgcta
     4621 ttgtaaccag ttctaaaagc tgtatttgag tttatcaccc ttgtcactaa gaaaataaat
     4681 gcagggtaaa atttatatcc ttcttgtttt atgtttcggt ataaaacact aatatcaatt
     4741 tctgtggtta tactaaaagt cgtttgttgg ttcaaataat gattaaatat ctcttttctc
     4801 ttccaattgt ctaaatcaat tttattaaag ttcatttgat atgcctccta aatttttatc
     4861 taaagtgaat ttaggaggct tacttgtctg ctttcttcat tagaatcaat ccttttttaa
     4921 aagtcaatat tactgtaaca taaatatata ttttaaaaat atcccacttt atccaatttt
     4981 cgtttgttga actaatgggt gctttagttg aagaataaaa gaccacatta aaaaatgtgg
     5041 tcttttgtgt ttttttaaag gatttgagcg tagcgaaaaa tccttttctt tcttatcttg
     5101 ataataaggg taactattgc cgatcgtcca ttccgacagc atcgccagtc actatggcgt
     5161 gctgctagcc aagcaagctt aaacccagct caatgagctg ggttttttgt ttgttgcagc
     5221 aatggcaaga acgtcccggg cggagctcag gccttaactc acattaattg cgttgcgctc
     5281 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5341 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     5401 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     5461 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     5521 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
     5581 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     5641 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     5701 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     5761 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     5821 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     5881 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     5941 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     6001 cactgacgcg ttgcgcgaga agattgtgca ccgccgtttt acaggcttcg acgccgcttc
     6061 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     6121 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     6181 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     6241 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     6301 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     6361 tcatcaaaat cgtctccctc cgtttgaata tttgattgat cgtaaccaga tgaagcactc
     6421 tttccactat ccctacagtg ttatggcttg aacaatcacg aaacaataat tggtacgtac
     6481 gatctttcag ccgactcaaa catcaaatct tacaaatgta gtctttgaaa gtattacata
     6541 tgtaagattt aaatgcaacc gttttttcgg aaggaaatga tgacctcgtt tccaccggaa
     6601 ttagcttggt accagctatt gtaacataat cggtacgggg gtgaaaaagc taacggaaaa
     6661 gggagcgaat ggcaagaacg tcccggggag ctcctaactt ataggggtaa cacttaaaaa
     6721 agaatcaata acgatagaaa ccgctcctaa agcaggtgca ttttttccta acgaagaagg
     6781 caatagttca catttattgt ctaaatgaga atggactcta gaagaaactt cgtttttaat
     6841 cgtatttaaa acaatgggat gagattcaat tatatgattt ctcaagataa cagcttctat
     6901 atcaaatgta ttaaggatat tggttaatcc aattccgata taaaagccaa agttttgaag
     6961 tgcatttaac atttctacat catttttatt tgcgcgttcc acaatctctt ttcgagaaat
     7021 attcttttct tctttagaga gcgaagccag taacgctttt tcagaagcat ataattccca
     7081 acagcctcga tttccacagc tgcatttggg tccattaaaa tctatcgtca tatgacccat
     7141 ttccccagaa aaaccctgaa cacctttata caattcgttg ttaataacaa gtccagttcc
     7201 aattccgata ttaatactga tgtaaacgat gttttcatag ttttttgtca taccaaatac
     7261 tttttcaccg tatgctcctg cattagcttc attttcaaca aaaaccggaa cattaaactc
     7321 actctcaatt aaaaactgca aatctttgat attccaattt aagttaggca tgaaaataat
     7381 ttgctgatga cgatctacaa ggcctggaac acaaattcct attccgacta gaccataagg
     7441 ggactcaggc atatgggtta caaaaccatg aataagtgca aataaaatct cttttacttc
     7501 actagcggaa gaactagaca agtcagaagt cttctcgaga ataatatttc cttctaagtc
     7561 ggttagaatt ccgttaagat agtcgactcc tatatcaata ccaatcgagt agcctgcatt
     7621 cttattaaaa acaagcatta caggtcttct gccgcctcta gattgccctg ccccaatttc
     7681 aaaaataaaa tctttttcaa gcagtgtatt tacttgagag gagacagtag acttgtttaa
     7741 tcctgtaatc tcagagagag ttgccctgga gacaggggag ttcttcaaaa tttcatctaa
     7801 tattaatttt tgattcattt tttttactaa agcttgatct gcaatttgaa taataaccac
     7861 tcctttgttt atccaccgaa ctaagttggt gttttttgaa gcttgaatta gatatttaaa
     7921 agtatcatat ctaatattat aactaaattt tctaaaaaaa acattgaaat aaacatttat
     7981 tttgtatatg atgagataaa gttagtttat tggataaaca aactaactca attaagatag
     8041 ttgatggata aacttgttca cttaaatcaa agggggaaat gacaaatggt ccaaactagt
     8101 gatatctaaa aatcaaaggg ggaaatggga tccatgtcaa tttatcaaga atttgttaat
     8161 aaatatagtt taagtaaaac tctaagattt gagttaatcc cacagggtaa aacacttgaa
     8221 aacataaaag caagaggttt gattttagat gatgagaaaa gagctaaaga ctacaaaaag
     8281 gctaaacaaa taattgataa atatcatcag ttttttatag aggagatatt aagttcggtt
     8341 tgtattagcg aagatttatt acaaaactat tctgatgttt attttaaact taaaaagagt
     8401 gatgatgata atctacaaaa agattttaaa agtgcaaaag atacgataaa gaaacaaata
     8461 tctgaatata taaaggactc agagaaattt aagaatttgt ttaatcaaaa ccttatcgat
     8521 gctaaaaaag ggcaagagtc agatttaatt ctatggctaa agcaatctaa ggataatggt
     8581 atagaactat ttaaagccaa tagtgatatc acagatatag atgaggcgtt agaaataatc
     8641 aaatctttta aaggttggac aacttatttt aagggttttc atgaaaatag aaaaaatgtt
     8701 tatagtagca atgatattcc tacatctatt atttatagga tagtagatga taatttgcct
     8761 aaatttctag aaaataaagc taagtatgag agtttaaaag acaaagctcc agaagctata
     8821 aactatgaac aaattaaaaa agatttggca gaagagctaa cctttgatat tgactacaaa
     8881 acatctgaag ttaatcaaag agttttttca cttgatgaag tttttgagat agcaaacttt
     8941 aataattatc taaatcaaag tggtattact aaatttaata ctattattgg tggtaaattt
     9001 gtaaatggtg aaaatacaaa gagaaaaggt ataaatgaat atataaatct atactcacag
     9061 caaataaatg ataaaacact caaaaaatat aaaatgagtg ttttatttaa gcaaatttta
     9121 agtgatacag aatctaaatc ttttgtaatt gataagttag aagatgatag tgatgtagtt
     9181 acaacgatgc aaagttttta tgagcaaata gcagctttta aaacagtaga agaaaaatct
     9241 attaaagaaa cactatcttt attatttgat gatttaaaag ctcaaaaact tgatttgagt
     9301 aaaatttatt ttaaaaatga taaatctctt actgatctat cacaacaagt ttttgatgat
     9361 tatagtgtta ttggtacagc ggtactagaa tatataactc aacaaatagc acctaaaaat
     9421 cttgataacc ctagtaagaa agagcaagaa ttaatagcca aaaaaactga aaaagcaaaa
     9481 tacttatctc tagaaactat aaagcttgcc ttagaagaat ttaataagca tagagatata
     9541 gataaacagt gtaggtttga agaaatactt gcaaactttg cggctattcc gatgatattt
     9601 gatgaaatag ctcaaaacaa agacaatttg gcacagatat ctatcaaata tcaaaatcaa
     9661 ggtaaaaaag acctacttca agctagtgcg gaagatgatg ttaaagctat caaggatctt
     9721 ttagatcaaa ctaataatct cttacataaa ctaaaaatat ttcatattag tcagtcagaa
     9781 gataaggcaa atattttaga caaggatgag catttttatc tagtatttga ggagtgctac
     9841 tttgagctag cgaatatagt gcctctttat aacaaaatta gaaactatat aactcaaaag
     9901 ccatatagtg atgagaaatt taagctcaat tttgagaact cgactttggc taatggttgg
     9961 gataaaaata aagagcctga caatacggca attttattta tcaaagatga taaatattat
    10021 ctgggtgtga tgaataagaa aaataacaaa atatttgatg ataaagctat caaagaaaat
    10081 aaaggcgagg gttataaaaa aattgtttat aaacttttac ctggcgcaaa taaaatgtta
    10141 cctaaggttt tcttttctgc taaatctata aaattttata atcctagtga agatatactt
    10201 agaataagaa atcattccac acatacaaaa aatggtagtc ctcaaaaagg atatgaaaaa
    10261 tttgagttta atattgaaga ttgccgaaaa tttatagatt tttataaaca gtctataagt
    10321 aagcatccgg agtggaaaga ttttggattt agattttctg atactcaaag atataattct
    10381 atagatgaat tttatagaga agttgaaaat caaggctaca aactaacttt tgaaaatata
    10441 tcagagagct atattgatag cgtagttaat cagggtaaat tgtacctatt ccaaatctat
    10501 aataaagatt tttcagctta tagcaaaggg cgaccaaatc tacatacttt atattggaaa
    10561 gcgctgtttg atgagagaaa tcttcaagat gtggtttata agctaaatgg tgaggcagag
    10621 cttttttatc gtaaacaatc aatacctaaa aaaatcactc acccagctaa agaggcaata
    10681 gctaataaaa acaaagataa tcctaaaaaa gagagtgttt ttgaatatga tttaatcaaa
    10741 gataaacgct ttactgaaga taagtttttc tttcactgtc ctattacaat caattttaaa
    10801 tctagtggag ctaataagtt taatgatgaa atcaatttat tgctaaaaga aaaagcaaat
    10861 gatgttcata tattaagtat agatagaggt gaaagacatt tagcttacta tactttggta
    10921 gatggtaaag gcaatatcat caaacaagat actttcaaca tcattggtaa tgatagaatg
    10981 aaaacaaact accatgataa gcttgctgca atagagaaag atagggattc agctaggaaa
    11041 gactggaaaa agataaataa catcaaagag atgaaagagg gctatctatc tcaggtagtt
    11101 catgaaatag ctaagctagt tatagagtat aatgctattg tggtttttga ggatttaaat
    11161 tttggattta aaagagggcg tttcaaggta gagaagcagg tctatcaaaa gttagaaaaa
    11221 atgctaattg agaaactaaa ctatctagtt ttcaaagata atgagtttga taaaactggg
    11281 ggagtgctta gagcttatca gctaacagca ccttttgaga cttttaaaaa gatgggtaaa
    11341 caaacaggta ttatctacta tgtaccagct ggttttactt caaaaatttg tcctgtaact
    11401 ggttttgtaa atcagttata tcctaagtat gaaagtgtca gcaaatctca agagttcttt
    11461 agtaagtttg acaagatttg ttataacctt gataagggct attttgagtt tagttttgat
    11521 tataaaaact ttggtgacaa ggctgccaaa ggcaagtgga ctatagctag ctttgggagt
    11581 agattgatta actttagaaa ttcagataaa aatcataatt gggatactcg agaagtttat
    11641 ccaactaaag agttggagaa attgctaaaa gattattcta tcgaatatgg gcatggcgaa
    11701 tgtatcaaag cagctatttg cggtgagagc gacaaaaagt tttttgctaa gctaactagt
    11761 gtcctaaata ctatcttaca aatgcgtaac tcaaaaacag gtactgagtt agattatcta
    11821 atttcaccag tagcagatgt aaatggcaat ttctttgatt cgcgacaggc gccaaaaaat
    11881 atgcctcaag atgctgatgc caatggtgct tatcatattg ggctaaaagg tctgatgcta
    11941 ctaggtagga tcaaaaataa tcaagagggc aaaaaactca atttggttat caaaaatgaa
    12001 gagtattttg agttcgtgca gaataggaat aactaactgc agaacgctcg gttgccgccg
    12061 ggcgtttttt cgtcatcgcg gaaaagaatg atgtaagcgt gaaaaatttt ttaaaaaatc
    12121 tcttgacatt ggaagggaga tatgttatta taagaattgc ggaattgtga gcggataaca
    12181 attcccaatt aaaggaggtg ggatccatgc atgcgatgcc tggggacgca gatatgttca
    12241 ttgggattgc ggtctctcgg agctaccccg aggacggtgc cagcggccag ataaacattg
    12301 ccgcgacggc gaccgccgtc tacaaggatg gaactatcct cggccactcg tccacccgac
    12361 cgcagctcgg ggagaaacta cagtcgacgg atgttcgtga cattatgaag aatgccatcc
    12421 tcggctacca gcaggtgacc ggtgagtcgc cgacccatat cgtcatccac cgtgcgggct
    12481 tcatgaacga agacctcgac cccgccacgg aattcctcaa cgaacaaggc gtcgagtacg
    12541 acatcgtcga aatccgcaag cagccccaga cacgcctgct ggcagtctcc gatgtgcagt
    12601 acgatacgcc tgtgaagagc atcgccgcta tcaaccagaa cgagccacgg gcaacggtcg
    12661 ccaccttcgg cgcacccgaa tacttagcga cacgcgatgg aggcggcctt ccccgcccaa
    12721 tccaaattga acgagtcgcc ggcgaaaccg acatcgagac gctcactcgc caagtctatc
    12781 tgctctccca gtcgcatatc caggtccata actcgactgc gcgcctaccc atcaccaccg
    12841 catacgccga ccaggcaagt actcacgcga ccaagggtta cctcgtccag accggagcgt
    12901 tcgagtctaa tgtcggattc ctctaatcct ctagagtcga cgtccccggg gcagcccgcc
    12961 taatgagcgg gcttttttca cgtcacgcgt ccatggagat ctttgtctgc aactgaaaag
    13021 tttatacctt acctggaaca aatggttgaa acatacgagg ctaatatcgg cttattagga
    13081 atagtccctg tactaataaa atcaggtgga tcagttgatc agtatatttt ggacgaagct
    13141 cggaaagaat ttggagatga cttgcttaat tccacaatta aattaaggga aagaataaag
    13201 cgatttgatg ttcaaggaat cacggaagaa gatactcatg ataaagaagc tctaaaacta
    13261 ttcaataacc ttacaatgga attgatcgaa agggtggaag gttaatggta cgaaaattag
    13321 gggatctacc tagaaagcca caaggcgata ggtcaagctt aaagaaccct tacatggatc
    13381 ttacagattc tgaaagtaaa gaaacaacag aggttaaaca aacagaacca aaaagaaaaa
    13441 aagcattgtt gaaaacaatg aaagttgatg tttcaatcca taataagatt aaatcgctgc
    13501 acgaaattct ggcagcatcc gaagggaatt catattactt agaggatact attgagagag
    13561 ctattgataa gatggttgag acattacctg agagccaaaa aactttttat gaatatgaat
    13621 taaaaaaaag aaccaacaaa ggctgagaca gactccaaac gagtctgttt ttttaaaaaa
    13681 aatattagga gcattgaata tatattagag aattaagaaa gacatgggaa taaaaatatt
    13741 ttaaatccag taaaaatatg ataagattat ttcagaatat gaagaactct gtttgttttt
    13801 gatgaaaaaa caaacaaaaa aaatccacct aacggaatct caatttaact aacagcggcc
    13861 aaactgagaa gttaaatttg agaaggggaa aaggcggatt tatacttgta tttaactatc
    13921 tccattttaa cattttatta aaccccatac aagtgaaaat cctcttttac actgttcctt
    13981 taggtgatcg cggagggaca tt
//

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