出品公司: | Molecularcloud |
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载体名称: | pHT-XCR6 |
质粒类型: | 枯草芽孢杆菌表达载体,穿梭载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | -- |
载体大小: | 14 kb |
5’测序引物: | -- |
3’测序引物: | -- |
载体抗性: | 氨苄青霉素 |
克隆菌株: | DH5 alpha |
宿主细胞(系): | 枯草芽孢杆菌 |
产品目录号: | MC_0068418 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
备注: | The plasmid is used for the expression of Cpf1 and NgAgo in B. subtilis,and can replicate in E. coli. |
参考文献: | Wu, Y., Liu, Y., Lv, X., Li, J., Du, G., & Liu, L. (2020). CAMERS‐B: CRISPR/Cpf1 assisted multiple‐genes editing and regulation system for Bacillus subtilis. Biotechnology and Bioengineering. |
买家导航
pHT-XCR6载体质粒基本信息
pHT-XCR6质粒图谱载体图谱和pHT-XCR6载体序列质粒序列多克隆位点信息
pHT-XCR6质粒载体简介
pHT-XCR6质粒序列载体序列
LOCUS pHT-XCR6 14002 bp DNA circular SYN 12-NOV-2024 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14002) AUTHORS Ming TITLE Direct Submission JOURNAL Exported Nov 12, 2024 from SnapGene 6.0.2 to Vector NTI(R) format https://www.biofeng.com COMMENT VNTDATE|1073779200| COMMENT VNTDBDATE|1073779200| COMMENT VNTNAME|pHT-XCR6| COMMENT VNTAUTHORNAME|Ming| FEATURES Location/Qualifiers promoter 2151..2255 /gene="bla" /label=AmpR promoter CDS 2256..3116 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" rep_origin 3287..3875 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5279..6361) /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 12161..12185 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 atgagtgaag taaacctaaa aggaaataca gatgaattag tgtattatcg acagcaaacc 61 actggaaata aaatcgccag gaagagaatc aaaaaaggga aagaagaagt ttattatgtt 121 gctgaaacgg aagagaagat atggacagaa gagcaaataa aaaacttttc tttagacaaa 181 tttggtacgc atatacctta catagaaggt cattatacaa tcttaaataa ttacttcttt 241 gatttttggg gctatttttt aggtgctgaa ggaattgcgc tctatgctca cctaactcgt 301 tatgcatacg gcagcaaaga cttttgcttt cctagtctac aaacaatcgc taaaaaaatg 361 gacaagactc ctgttacagt tagaggctac ttgaaactgc ttgaaaggta cggttttatt 421 tggaaggtaa acgtccgtaa taaaaccaag gataacacag aggaatcccc gatttttaag 481 attagacgta aggttccttt gctttcagaa gaacttttaa atggaaaccc taatattgaa 541 attccagatg acgaggaagc acatgtaaag aaggctttaa aaaaggaaaa agagggtctt 601 ccaaaggttt tgaaaaaaga gcacgatgaa tttgttaaaa aaatgatgga tgagtcagaa 661 acaattaata ttccagaggc cttacaatat gacacaatgt atgaagatat actcagtaaa 721 ggagaaattc gaaaagaaat caaaaaacaa atacctaatc ctacaacatc ttttgagagt 781 atatcaatga caactgaaga ggaaaaagtc gacagtactt taaaaagcga aatgcaaaat 841 cgtgtctcta agccttcttt tgatacctgg tttaaaaaca ctaagatcaa aattgaaaat 901 aaaaattgtt tattacttgt accgagtgaa tttgcatttg aatggattaa gaaaagatat 961 ttagaaacaa ttaaaacagt ccttgaagaa gctggatatg ttttcgaaaa aatcgaacta 1021 agaaaagtgc aataaactgc tgaagtattt cagcagtttt ttttatttag aaatagtgaa 1081 aaaaatataa tcagggaggt atcaatattt aatgagtact gatttaaatt tatttagact 1141 ggaattaata attaacacgt agactaatta aaatttaatg agggataaag aggatacaaa 1201 aatattaatt tcaatcccta ttaaatttta acaagggggg gattaaaatt taattagagg 1261 tttatccaca agaaaagacc ctaataaaat ttttactagg gttataacac tgattaattt 1321 cttaatgggg gagggattaa aatttaatga caaagaaaac aatcttttaa gaaaagcttt 1381 taaaagataa taataaaaag agctttgcga ttaagcaaaa ctctttactt tttcattgac 1441 attatcaaat tcatcgattt caaattgttg ttgtatcata aagttaattc tgttttgcac 1501 aaccttttca ggaatataaa acacatctga ggcttgtttt ataaactcag ggtcgctaaa 1561 gtcaatgtaa cgtagcatat gatatggtat agcttccacc caagttagcc tttctgcttc 1621 ttctgaatgt ttttcatata cttccatggg tatctctaaa tgattttcct catgtagcaa 1681 ggtatgagca aaaagtttat ggaattgata gttcctctct ttttcttcaa cttttttatc 1741 taaaacaaac actttaacat ctgagtcaat gtaagcataa gatgtttttc cagtcataat 1801 ttcaatccca aatcttttag acagaaattc tggacgtaaa tcttttggtg aaagaatttt 1861 tttatgtagc aatatatccg atacagcacc ttctaaaagc gttggtgaat agggcatttt 1921 acctatctcc tctcattttg tggaataaaa atagtcatat tcgtccatct acctatccta 1981 ttatcgaaca gttgaacttt ttaatcaagg atcagtcctt tttttcatta ttcttaaact 2041 gtgctcttaa ctttaacaac tcgatttgtt tttccagatc tcgagggtaa ctagcctcgc 2101 cgatcccgca agaggcccgg cagtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 2161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 2221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 2281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 2341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 2401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 2461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 2521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 2581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 2641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 2701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 2761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 2821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 2881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 2941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 3001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 3061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 3121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 3181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 3241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 3301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 3361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 3421 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 3481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 3541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 3601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 3661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 3721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 3901 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 3961 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 4021 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc atgcttaagt 4081 tattggtatg actggtttta agcgcaaaaa aagttgcttt ttcgtaccta ttaatgtatc 4141 gttagaaaac cgactgtaaa aagtacagtc ggcattatct catattataa aagccagtca 4201 ttaggcctat ctgacaattc ctgaatagag ttcataaaca atcctgcatg ataaccatca 4261 caaacagaat gatgtacctg taaagatagc ggtaaatata ttgaattacc tttattaatg 4321 aattttcctg ctgtaataat gggtagaagg taattactat tattattgat atttaagtta 4381 aacccagtaa atgaagtcca tggaataata gaaagagaaa aagcattttc aggtataggt 4441 gttttgggaa acaatttccc cgaaccatta tatttctcta catcagaaag gtataaatca 4501 taaaactctt tgaagtcatt ctttacagga gtccaaatac cagagaatgt tttagataca 4561 ccatcaaaaa ttgtataaag tggctctaac ttatcccaat aacctaactc tccgtcgcta 4621 ttgtaaccag ttctaaaagc tgtatttgag tttatcaccc ttgtcactaa gaaaataaat 4681 gcagggtaaa atttatatcc ttcttgtttt atgtttcggt ataaaacact aatatcaatt 4741 tctgtggtta tactaaaagt cgtttgttgg ttcaaataat gattaaatat ctcttttctc 4801 ttccaattgt ctaaatcaat tttattaaag ttcatttgat atgcctccta aatttttatc 4861 taaagtgaat ttaggaggct tacttgtctg ctttcttcat tagaatcaat ccttttttaa 4921 aagtcaatat tactgtaaca taaatatata ttttaaaaat atcccacttt atccaatttt 4981 cgtttgttga actaatgggt gctttagttg aagaataaaa gaccacatta aaaaatgtgg 5041 tcttttgtgt ttttttaaag gatttgagcg tagcgaaaaa tccttttctt tcttatcttg 5101 ataataaggg taactattgc cgatcgtcca ttccgacagc atcgccagtc actatggcgt 5161 gctgctagcc aagcaagctt aaacccagct caatgagctg ggttttttgt ttgttgcagc 5221 aatggcaaga acgtcccggg cggagctcag gccttaactc acattaattg cgttgcgctc 5281 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 5341 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga 5401 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca 5461 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac 5521 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc 5581 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg 5641 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac 5701 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc 5761 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt 5821 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga 5881 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag 5941 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc 6001 cactgacgcg ttgcgcgaga agattgtgca ccgccgtttt acaggcttcg acgccgcttc 6061 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg 6121 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg 6181 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg 6241 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg 6301 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca 6361 tcatcaaaat cgtctccctc cgtttgaata tttgattgat cgtaaccaga tgaagcactc 6421 tttccactat ccctacagtg ttatggcttg aacaatcacg aaacaataat tggtacgtac 6481 gatctttcag ccgactcaaa catcaaatct tacaaatgta gtctttgaaa gtattacata 6541 tgtaagattt aaatgcaacc gttttttcgg aaggaaatga tgacctcgtt tccaccggaa 6601 ttagcttggt accagctatt gtaacataat cggtacgggg gtgaaaaagc taacggaaaa 6661 gggagcgaat ggcaagaacg tcccggggag ctcctaactt ataggggtaa cacttaaaaa 6721 agaatcaata acgatagaaa ccgctcctaa agcaggtgca ttttttccta acgaagaagg 6781 caatagttca catttattgt ctaaatgaga atggactcta gaagaaactt cgtttttaat 6841 cgtatttaaa acaatgggat gagattcaat tatatgattt ctcaagataa cagcttctat 6901 atcaaatgta ttaaggatat tggttaatcc aattccgata taaaagccaa agttttgaag 6961 tgcatttaac atttctacat catttttatt tgcgcgttcc acaatctctt ttcgagaaat 7021 attcttttct tctttagaga gcgaagccag taacgctttt tcagaagcat ataattccca 7081 acagcctcga tttccacagc tgcatttggg tccattaaaa tctatcgtca tatgacccat 7141 ttccccagaa aaaccctgaa cacctttata caattcgttg ttaataacaa gtccagttcc 7201 aattccgata ttaatactga tgtaaacgat gttttcatag ttttttgtca taccaaatac 7261 tttttcaccg tatgctcctg cattagcttc attttcaaca aaaaccggaa cattaaactc 7321 actctcaatt aaaaactgca aatctttgat attccaattt aagttaggca tgaaaataat 7381 ttgctgatga cgatctacaa ggcctggaac acaaattcct attccgacta gaccataagg 7441 ggactcaggc atatgggtta caaaaccatg aataagtgca aataaaatct cttttacttc 7501 actagcggaa gaactagaca agtcagaagt cttctcgaga ataatatttc cttctaagtc 7561 ggttagaatt ccgttaagat agtcgactcc tatatcaata ccaatcgagt agcctgcatt 7621 cttattaaaa acaagcatta caggtcttct gccgcctcta gattgccctg ccccaatttc 7681 aaaaataaaa tctttttcaa gcagtgtatt tacttgagag gagacagtag acttgtttaa 7741 tcctgtaatc tcagagagag ttgccctgga gacaggggag ttcttcaaaa tttcatctaa 7801 tattaatttt tgattcattt tttttactaa agcttgatct gcaatttgaa taataaccac 7861 tcctttgttt atccaccgaa ctaagttggt gttttttgaa gcttgaatta gatatttaaa 7921 agtatcatat ctaatattat aactaaattt tctaaaaaaa acattgaaat aaacatttat 7981 tttgtatatg atgagataaa gttagtttat tggataaaca aactaactca attaagatag 8041 ttgatggata aacttgttca cttaaatcaa agggggaaat gacaaatggt ccaaactagt 8101 gatatctaaa aatcaaaggg ggaaatggga tccatgtcaa tttatcaaga atttgttaat 8161 aaatatagtt taagtaaaac tctaagattt gagttaatcc cacagggtaa aacacttgaa 8221 aacataaaag caagaggttt gattttagat gatgagaaaa gagctaaaga ctacaaaaag 8281 gctaaacaaa taattgataa atatcatcag ttttttatag aggagatatt aagttcggtt 8341 tgtattagcg aagatttatt acaaaactat tctgatgttt attttaaact taaaaagagt 8401 gatgatgata atctacaaaa agattttaaa agtgcaaaag atacgataaa gaaacaaata 8461 tctgaatata taaaggactc agagaaattt aagaatttgt ttaatcaaaa ccttatcgat 8521 gctaaaaaag ggcaagagtc agatttaatt ctatggctaa agcaatctaa ggataatggt 8581 atagaactat ttaaagccaa tagtgatatc acagatatag atgaggcgtt agaaataatc 8641 aaatctttta aaggttggac aacttatttt aagggttttc atgaaaatag aaaaaatgtt 8701 tatagtagca atgatattcc tacatctatt atttatagga tagtagatga taatttgcct 8761 aaatttctag aaaataaagc taagtatgag agtttaaaag acaaagctcc agaagctata 8821 aactatgaac aaattaaaaa agatttggca gaagagctaa cctttgatat tgactacaaa 8881 acatctgaag ttaatcaaag agttttttca cttgatgaag tttttgagat agcaaacttt 8941 aataattatc taaatcaaag tggtattact aaatttaata ctattattgg tggtaaattt 9001 gtaaatggtg aaaatacaaa gagaaaaggt ataaatgaat atataaatct atactcacag 9061 caaataaatg ataaaacact caaaaaatat aaaatgagtg ttttatttaa gcaaatttta 9121 agtgatacag aatctaaatc ttttgtaatt gataagttag aagatgatag tgatgtagtt 9181 acaacgatgc aaagttttta tgagcaaata gcagctttta aaacagtaga agaaaaatct 9241 attaaagaaa cactatcttt attatttgat gatttaaaag ctcaaaaact tgatttgagt 9301 aaaatttatt ttaaaaatga taaatctctt actgatctat cacaacaagt ttttgatgat 9361 tatagtgtta ttggtacagc ggtactagaa tatataactc aacaaatagc acctaaaaat 9421 cttgataacc ctagtaagaa agagcaagaa ttaatagcca aaaaaactga aaaagcaaaa 9481 tacttatctc tagaaactat aaagcttgcc ttagaagaat ttaataagca tagagatata 9541 gataaacagt gtaggtttga agaaatactt gcaaactttg cggctattcc gatgatattt 9601 gatgaaatag ctcaaaacaa agacaatttg gcacagatat ctatcaaata tcaaaatcaa 9661 ggtaaaaaag acctacttca agctagtgcg gaagatgatg ttaaagctat caaggatctt 9721 ttagatcaaa ctaataatct cttacataaa ctaaaaatat ttcatattag tcagtcagaa 9781 gataaggcaa atattttaga caaggatgag catttttatc tagtatttga ggagtgctac 9841 tttgagctag cgaatatagt gcctctttat aacaaaatta gaaactatat aactcaaaag 9901 ccatatagtg atgagaaatt taagctcaat tttgagaact cgactttggc taatggttgg 9961 gataaaaata aagagcctga caatacggca attttattta tcaaagatga taaatattat 10021 ctgggtgtga tgaataagaa aaataacaaa atatttgatg ataaagctat caaagaaaat 10081 aaaggcgagg gttataaaaa aattgtttat aaacttttac ctggcgcaaa taaaatgtta 10141 cctaaggttt tcttttctgc taaatctata aaattttata atcctagtga agatatactt 10201 agaataagaa atcattccac acatacaaaa aatggtagtc ctcaaaaagg atatgaaaaa 10261 tttgagttta atattgaaga ttgccgaaaa tttatagatt tttataaaca gtctataagt 10321 aagcatccgg agtggaaaga ttttggattt agattttctg atactcaaag atataattct 10381 atagatgaat tttatagaga agttgaaaat caaggctaca aactaacttt tgaaaatata 10441 tcagagagct atattgatag cgtagttaat cagggtaaat tgtacctatt ccaaatctat 10501 aataaagatt tttcagctta tagcaaaggg cgaccaaatc tacatacttt atattggaaa 10561 gcgctgtttg atgagagaaa tcttcaagat gtggtttata agctaaatgg tgaggcagag 10621 cttttttatc gtaaacaatc aatacctaaa aaaatcactc acccagctaa agaggcaata 10681 gctaataaaa acaaagataa tcctaaaaaa gagagtgttt ttgaatatga tttaatcaaa 10741 gataaacgct ttactgaaga taagtttttc tttcactgtc ctattacaat caattttaaa 10801 tctagtggag ctaataagtt taatgatgaa atcaatttat tgctaaaaga aaaagcaaat 10861 gatgttcata tattaagtat agatagaggt gaaagacatt tagcttacta tactttggta 10921 gatggtaaag gcaatatcat caaacaagat actttcaaca tcattggtaa tgatagaatg 10981 aaaacaaact accatgataa gcttgctgca atagagaaag atagggattc agctaggaaa 11041 gactggaaaa agataaataa catcaaagag atgaaagagg gctatctatc tcaggtagtt 11101 catgaaatag ctaagctagt tatagagtat aatgctattg tggtttttga ggatttaaat 11161 tttggattta aaagagggcg tttcaaggta gagaagcagg tctatcaaaa gttagaaaaa 11221 atgctaattg agaaactaaa ctatctagtt ttcaaagata atgagtttga taaaactggg 11281 ggagtgctta gagcttatca gctaacagca ccttttgaga cttttaaaaa gatgggtaaa 11341 caaacaggta ttatctacta tgtaccagct ggttttactt caaaaatttg tcctgtaact 11401 ggttttgtaa atcagttata tcctaagtat gaaagtgtca gcaaatctca agagttcttt 11461 agtaagtttg acaagatttg ttataacctt gataagggct attttgagtt tagttttgat 11521 tataaaaact ttggtgacaa ggctgccaaa ggcaagtgga ctatagctag ctttgggagt 11581 agattgatta actttagaaa ttcagataaa aatcataatt gggatactcg agaagtttat 11641 ccaactaaag agttggagaa attgctaaaa gattattcta tcgaatatgg gcatggcgaa 11701 tgtatcaaag cagctatttg cggtgagagc gacaaaaagt tttttgctaa gctaactagt 11761 gtcctaaata ctatcttaca aatgcgtaac tcaaaaacag gtactgagtt agattatcta 11821 atttcaccag tagcagatgt aaatggcaat ttctttgatt cgcgacaggc gccaaaaaat 11881 atgcctcaag atgctgatgc caatggtgct tatcatattg ggctaaaagg tctgatgcta 11941 ctaggtagga tcaaaaataa tcaagagggc aaaaaactca atttggttat caaaaatgaa 12001 gagtattttg agttcgtgca gaataggaat aactaactgc agaacgctcg gttgccgccg 12061 ggcgtttttt cgtcatcgcg gaaaagaatg atgtaagcgt gaaaaatttt ttaaaaaatc 12121 tcttgacatt ggaagggaga tatgttatta taagaattgc ggaattgtga gcggataaca 12181 attcccaatt aaaggaggtg ggatccatgc atgcgatgcc tggggacgca gatatgttca 12241 ttgggattgc ggtctctcgg agctaccccg aggacggtgc cagcggccag ataaacattg 12301 ccgcgacggc gaccgccgtc tacaaggatg gaactatcct cggccactcg tccacccgac 12361 cgcagctcgg ggagaaacta cagtcgacgg atgttcgtga cattatgaag aatgccatcc 12421 tcggctacca gcaggtgacc ggtgagtcgc cgacccatat cgtcatccac cgtgcgggct 12481 tcatgaacga agacctcgac cccgccacgg aattcctcaa cgaacaaggc gtcgagtacg 12541 acatcgtcga aatccgcaag cagccccaga cacgcctgct ggcagtctcc gatgtgcagt 12601 acgatacgcc tgtgaagagc atcgccgcta tcaaccagaa cgagccacgg gcaacggtcg 12661 ccaccttcgg cgcacccgaa tacttagcga cacgcgatgg aggcggcctt ccccgcccaa 12721 tccaaattga acgagtcgcc ggcgaaaccg acatcgagac gctcactcgc caagtctatc 12781 tgctctccca gtcgcatatc caggtccata actcgactgc gcgcctaccc atcaccaccg 12841 catacgccga ccaggcaagt actcacgcga ccaagggtta cctcgtccag accggagcgt 12901 tcgagtctaa tgtcggattc ctctaatcct ctagagtcga cgtccccggg gcagcccgcc 12961 taatgagcgg gcttttttca cgtcacgcgt ccatggagat ctttgtctgc aactgaaaag 13021 tttatacctt acctggaaca aatggttgaa acatacgagg ctaatatcgg cttattagga 13081 atagtccctg tactaataaa atcaggtgga tcagttgatc agtatatttt ggacgaagct 13141 cggaaagaat ttggagatga cttgcttaat tccacaatta aattaaggga aagaataaag 13201 cgatttgatg ttcaaggaat cacggaagaa gatactcatg ataaagaagc tctaaaacta 13261 ttcaataacc ttacaatgga attgatcgaa agggtggaag gttaatggta cgaaaattag 13321 gggatctacc tagaaagcca caaggcgata ggtcaagctt aaagaaccct tacatggatc 13381 ttacagattc tgaaagtaaa gaaacaacag aggttaaaca aacagaacca aaaagaaaaa 13441 aagcattgtt gaaaacaatg aaagttgatg tttcaatcca taataagatt aaatcgctgc 13501 acgaaattct ggcagcatcc gaagggaatt catattactt agaggatact attgagagag 13561 ctattgataa gatggttgag acattacctg agagccaaaa aactttttat gaatatgaat 13621 taaaaaaaag aaccaacaaa ggctgagaca gactccaaac gagtctgttt ttttaaaaaa 13681 aatattagga gcattgaata tatattagag aattaagaaa gacatgggaa taaaaatatt 13741 ttaaatccag taaaaatatg ataagattat ttcagaatat gaagaactct gtttgttttt 13801 gatgaaaaaa caaacaaaaa aaatccacct aacggaatct caatttaact aacagcggcc 13861 aaactgagaa gttaaatttg agaaggggaa aaggcggatt tatacttgta tttaactatc 13921 tccattttaa cattttatta aaccccatac aagtgaaaat cctcttttac actgttcctt 13981 taggtgatcg cggagggaca tt //
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