| 质粒名称: | pCAG-VSVP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 64088 |
| 质粒用途: | 水泡性口炎病毒phosphoprotein基因的表达载体 |
| 存储实验室: | Ian Wickersham |
| 相关质粒: |
pCAG-VSVG pCAG-VSVL pCAG-VSVM pCAG-VSVN pCAG-VSVP |
|
载体骨架基本信息 |
|
| 载体名称: | pCAGEN |
| 空载体大小: | 4795 bp |
| 完整质粒大小: | 5593 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | VSV phosphoprotein |
| 物种来源: | Vesicular Stomatitis Virus |
| 插入大小: | 798 bp |
| 启动子: | CAG |
| 克隆方法: | 未知 |
| 5’测序引物 | CTCTAGAGCCTCTGCTAACc |
| 3’测序引物 | CAGAAGTCAGATGCTCAAG |
|
文献引用方法 |
|
| 材料和方法部分: | pCAG-VSVG was a gift from Ian Wickersham (Addgene plasmid # 64084 ; http://n2t.net/addgene:64084 ; RRID:Addgene_64084) |
| 参考文献部分: | 未发表 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAG-VSVP 5593 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression vector for Vesicular Stomatitis Virus Indiana strain
phosphoprotein gene.
ACCESSION .
VERSION .
KEYWORDS pCAG-VSVP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5593)
TITLE Genetic Neuroengineering Group Plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5593)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5593
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 386..661
/label=chicken beta-actin promoter
intron 662..1670
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1594..1616
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1678..1697
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 1728..1737
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(2617..2636)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 2682..2737
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(2736..2755)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3096..3112)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3096..3112)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3109..3131)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3120..3136
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3144..3174)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3189..3210
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3269..3464
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 3315..3450
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3377..3396
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 3470..3604
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3507..3526)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3561..3580
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3672..3689)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3843..4431)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3923..3942)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4602..5462)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5225..5244
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5463..5567)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccgcc accatggata
1741 atctcacaaa agttcgtgag tatctcaagt cctactctcg tctagatcag gcggtaggag
1801 agatagatga gatcgaagca caacgagctg aaaagtccaa ttatgagttg ttccaagagg
1861 acggagtgga agagcatact aggccctctt attttcaggc agcagatgat tctgacacag
1921 aatctgaacc agaaattgaa gacaatcaag gcttgtatgt accagatccg gaagctgagc
1981 aagttgaagg ctttatacag gggcctttag atgactatgc agatgaggac gtggatgttg
2041 tattcacttc ggactggaaa cagcctgagc ttgaatccga cgagcatgga aagaccttac
2101 ggttgacatt gccagagggt ttaagtggag agcagaaatc ccagtggctt ttgacgatta
2161 aagcagtcgt tcaaagtgcc aaacactgga atctggcaga gtgcacattt gaagcatcgg
2221 gagaaggggt catcataaaa aagcgccaga taactccgga tgtatataag gtcactccag
2281 tgatgaacac acatccgtac caatcagaag ccgtatcaga tgtttggtct ctctcaaaga
2341 catccatgac tttccaaccc aagaaagcaa gtcttcagcc tctcaccata tccttggatg
2401 aattgttctc atctagagga gaattcatct ctgtcggagg taacggacga atgtctcata
2461 aagaggccat cctgctcggt ctgaggtaca aaaagttgta caatcaggcg agagtcaaat
2521 attctctgta ggcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
2581 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
2641 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
2701 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
2761 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg
2821 ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg
2881 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
2941 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
3001 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat
3061 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
3121 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
3181 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
3241 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
3301 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
3361 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
3421 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
3481 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
3541 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
3601 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
3661 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
3721 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
3781 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
3841 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
3901 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
3961 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
4021 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
4081 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
4141 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
4201 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
4261 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
4321 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
4381 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
4441 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
4501 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
4561 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
4621 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
4681 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
4741 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
4801 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
4861 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
4921 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
4981 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
5041 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
5101 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
5161 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
5221 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
5281 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
5341 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
5401 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
5461 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
5521 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
5581 aaagtgccac ctg
//
