| 质粒名称: | pCAG-VSVN |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 64087 |
| 质粒用途: | 水泡性口炎病毒nucleoprotein基因的表达载体 |
| 存储实验室: | Ian Wickersham |
| 相关质粒: |
pCAG-VSVG pCAG-VSVL pCAG-VSVM pCAG-VSVN pCAG-VSVP |
|
载体骨架基本信息 |
|
| 载体名称: | pCAGEN |
| 空载体大小: | 4795 bp |
| 完整质粒大小: | 6064 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | VSV nucleoprotein |
| 物种来源: | Vesicular Stomatitis Virus |
| 插入大小: | 1269 bp |
| 启动子: | CAG |
| 克隆方法: | 未知 |
| 5’测序引物 | CTCTAGAGCCTCTGCTAACc |
| 3’测序引物 | CAGAAGTCAGATGCTCAAG |
|
文献引用方法 |
|
| 材料和方法部分: | pCAG-VSVG was a gift from Ian Wickersham (Addgene plasmid # 64084 ; http://n2t.net/addgene:64084 ; RRID:Addgene_64084) |
| 参考文献部分: | 未发表 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAG-VSVN 6064 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression vector for Vesicular Stomatitis Virus Indiana strain
nucleoprotein gene.
ACCESSION .
VERSION .
KEYWORDS pCAG-VSVN
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6064)
TITLE Genetic Neuroengineering Group Plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6064)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6064
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 386..661
/label=chicken beta-actin promoter
intron 662..1670
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1594..1616
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1678..1697
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind complement(3088..3107)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3153..3208
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3207..3226)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3567..3583)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3567..3583)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3580..3602)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3591..3607
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3615..3645)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3660..3681
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3740..3935
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 3786..3921
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3848..3867
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 3941..4075
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3978..3997)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4032..4051
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4143..4160)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4314..4902)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4394..4413)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5073..5933)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5696..5715
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5934..6038)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccgcc accatgtctg
1741 ttacagtcaa gagaatcatt gacaacacag tcatagttcc aaaacttcct gcaaatgagg
1801 atccagtgga atacccggca gattacttca gaaaatcaaa ggagattcct ctttacatca
1861 atactacaaa aagtttgtca gatctaagag gatatgtcta ccaaggcctc aaatccggaa
1921 atgtatcaat catacatgtc aacagctact tgtatggagc attaaaggac atccggggta
1981 agttggataa agattggtca agtttcggaa taaacatcgg gaaagcaggg gatacaatcg
2041 gaatatttga ccttgtatcc ttgaaagccc tggacggcgt acttccagat ggagtatcgg
2101 atgcttccag aaccagcgca gatgacaaat ggttgccttt gtatctactt ggcttataca
2161 gagtgggcag aacacaaatg cctgaataca gaaaaaagct catggatggg ctgacaaatc
2221 aatgcaaaat gatcaatgaa cagtttgaac ctcttgtgcc agaaggtcgt gacatttttg
2281 atgtgtgggg aaatgacagt aattacacaa aaattgtcgc tgcagtggac atgttcttcc
2341 acatgttcaa aaaacatgaa tgtgcctcgt tcagatacgg aactattgtt tccagattca
2401 aagattgtgc tgcattggca acatttggac acctctgcaa aataaccgga atgtctacag
2461 aagatgtaac gacctggatc ttgaaccgag aagttgcaga tgaaatggtc caaatgatgc
2521 ttccaggcca agaaattgac aaggccgatt catacatgcc ttatttgatc gactttggat
2581 tgtcttctaa gtctccatat tcttccgtca aaaaccctgc cttccacttc tgggggcaat
2641 tgacagctct tctgctcaga tccaccagag caaggaatgc ccgacagcct gatgacattg
2701 agtatacatc tcttactaca gcaggtttgt tgtacgctta tgcagtagga tcctctgccg
2761 acttggcaca acagttttgt gttggagata acaaatacac tccagatgat agtaccggag
2821 gattgacgac taatgcaccg ccacaaggca gagatgtggt cgaatggctc ggatggtttg
2881 aagatcaaaa cagaaaaccg actcctgata tgatgcagta tgcgaaaaga gcagtcatgt
2941 cactgcaagg cctaagagag aagacaattg gcaagtatgc taagtcagaa tttgacaaat
3001 gagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
3061 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
3121 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
3181 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
3241 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc
3301 atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
3361 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
3421 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
3481 tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga tccctcgacc
3541 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
3601 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
3661 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
3721 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
3781 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
3841 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
3901 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
3961 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
4021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
4081 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
4141 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
4201 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
4261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
4321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
4381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
4441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
4501 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
4561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
4621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
4681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
4741 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
4801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
4861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
4921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
4981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
5041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
5101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
5161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
5221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
5281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
5341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
5401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
5461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
5521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
5581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
5641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
5701 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
5761 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
5821 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
5881 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
5941 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
6001 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
6061 cctg
//
