| 质粒名称: | pCAG-VSVM |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 64086 |
| 质粒用途: | 水泡性口炎病毒matrix基因的表达载体 |
| 存储实验室: | Ian Wickersham |
| 相关质粒: |
pCAG-VSVG pCAG-VSVL pCAG-VSVM pCAG-VSVN pCAG-VSVP |
|
载体骨架基本信息 |
|
| 载体名称: | pCAGEN |
| 空载体大小: | 4795 bp |
| 完整质粒大小: | 5485 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | VSV matrix protein |
| 物种来源: | Vesicular Stomatitis Virus |
| 插入大小: | 690 bp |
| 启动子: | CAG |
| 克隆方法: | 未知 |
| 5’测序引物 | CTCTAGAGCCTCTGCTAACc |
| 3’测序引物 | CAGAAGTCAGATGCTCAAG |
|
文献引用方法 |
|
| 材料和方法部分: | pCAG-VSVG was a gift from Ian Wickersham (Addgene plasmid # 64084 ; http://n2t.net/addgene:64084 ; RRID:Addgene_64084) |
| 参考文献部分: | 未发表 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAG-VSVM 5485 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression vector for Vesicular Stomatitis Virus Indiana strain
matrix gene.
ACCESSION .
VERSION .
KEYWORDS pCAG-VSVM
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5485)
TITLE Genetic Neuroengineering Group Plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5485)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5485
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 386..661
/label=chicken beta-actin promoter
intron 662..1670
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1594..1616
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1678..1697
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind complement(2509..2528)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 2574..2629
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(2628..2647)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(2988..3004)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2988..3004)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3001..3023)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3012..3028
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3036..3066)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3081..3102
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3161..3356
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 3207..3342
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3269..3288
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 3362..3496
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3399..3418)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3453..3472
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3564..3581)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3735..4323)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3815..3834)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4494..5354)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5117..5136
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5355..5459)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccgcc accatgagtt
1741 ccttaaagaa gattctcggt ctgaagggga aaggtaagaa atctaagaaa ttagggatcg
1801 caccaccccc ttatgaagag gacactagca tggagtatgc tccgagcgct ccaattgaca
1861 aatcctattt tggagttgac gagatggaca cctatgatcc gaatcaatta agatatgaga
1921 aattcttctt tacagtgaaa atgacggtta gatctaatcg tccgttcaga acatactcag
1981 atgtggcagc cgctgtatcc cattgggatc acatgtacat cggaatggca gggaaacgtc
2041 ccttctacaa aatcttggct tttttgggtt cttctaatct aaaggccact ccagcggtat
2101 tggcagatca aggtcaacca gagtatcacg ctcactgcga aggcagggct tatttgccac
2161 ataggatggg gaagacccct cccatgctca atgtaccaga gcacttcaga agaccattca
2221 atataggtct ttacaaggga acgattgagc tcacaatgac catctacgat gatgagtcac
2281 tggaagcagc tcctatgatc tgggatcatt tcaattcttc caaattttct gatttcagag
2341 agaaggcctt aatgtttggc ctgattgtcg agaaaaaggc atctggagcg tgggtcctgg
2401 attctatcag ccacttcaaa tgagcggccg cactcctcag gtgcaggctg cctatcagaa
2461 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
2521 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
2581 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
2641 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
2701 atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga
2761 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
2821 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
2881 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
2941 tcttatgaag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt
3001 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
3061 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
3121 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
3181 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
3241 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
3301 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
3361 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
3421 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
3481 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
3541 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
3601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
3661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
3721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
3781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
3841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
3901 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
3961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
4021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
4081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
4141 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
4201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
4261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
4321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
4381 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
4441 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
4501 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
4561 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
4621 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
4681 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
4741 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
4801 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
4861 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
4921 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
4981 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
5041 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
5101 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
5161 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
5221 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
5281 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
5341 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
5401 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
5461 acatttcccc gaaaagtgcc acctg
//
