质粒名称: | pCAG-GFP |
---|---|
出品公司: | Addgene |
目录编号: | 11150 |
存储实验室: | Connie Cepko |
载体骨架基本信息 |
|
载体名称: | pCAGEN |
载体出品公司: | Available at Addgene (#11160) |
载体抗性: | 氨苄青霉素 |
空载体大小: | 4779 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | -- |
筛选标记: | -- |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
|
插入基因名称: | EGFP from Aequorea victoria,GFP,green fluorescent protein |
物种来源: | -- |
插入大小: | 772 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | EcoRI(未破坏) |
3' 克隆位点: | NotI (未破坏) |
5' 测序引物 | pCAG-F |
3' 测序引物 | EGFP-N |
文献引用方法 |
|
材料和方法部分: | pCAG-GFP was a gift from Connie Cepko (Addgene plasmid # 11150 ; http://n2t.net/addgene:11150 ; RRID:Addgene_11150) |
参考文献部分: | Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. 10.1073/pnas.2235688100 PubMed 14603031 |
买家导航
pCAGGFP载体基本信息
pCAGGFP载体图谱质粒图谱和多克隆位点信息
pCAGGFP载体简介
pCAGGFP载体序列
LOCUS pCAG-GFP 5561 bp ds-DNA circular SYN 25-NOV-2019 DEFINITION Mammalian expression vector for expression of GFP (CAG promoter). ACCESSION . VERSION . KEYWORDS pCAG-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5561) AUTHORS Matsuda T, Cepko CL TITLE Electroporation and RNA interference in the rodent retina in vivo and in vitro. JOURNAL Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. PUBMED 14603031 REFERENCE 2 (bases 1 to 5561) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 25, 2019 from SnapGene Server 1.1.58 http://www.biofeng.com/ FEATURES Location/Qualifiers source 1..5561 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 5..384 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" intron 662..1678 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1602..1624 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1686..1705 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" regulatory 1771..1780 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1777..2496 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1822..1843) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2083..2102) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2430..2451 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(2583..2602) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_site 2648..2703 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal" primer_bind complement(2702..2721) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(3064..3080) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3064..3080) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3077..3099) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3088..3104 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3112..3142) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3157..3178 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3237..3432 /label=SV40 promoter /note="SV40 early promoter" rep_origin 3283..3418 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3345..3364 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 3438..3572 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3475..3494) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3529..3548 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3640..3657) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3811..4399) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3891..3910) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4570..5430) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5193..5212 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5431..5535) /gene="bla" /label=AmpR promoter ORIGIN 1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat 61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc 421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 481 gcgatggggg cggggggggg gggggsgcgc gccrggsggg gsggggsggg gsgrggggsg 541 gggsggggsg rggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc 721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc 781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga 841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg 901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg 961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg 1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt 1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca 1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg 1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 1261 ggggccgcct cgggccgggg rgggctcggg ggaggggcgc ggcggccccc ggagcgccgg 1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc 1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac 1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga 1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc 1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt 1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct 1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tctgcagtcg 1741 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg 1801 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 1861 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 1921 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc 1981 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 2041 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 2101 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 2161 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 2221 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 2281 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 2341 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg 2401 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 2461 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcactcc tcaggtgcag 2521 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga 2581 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 2641 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 2701 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt 2761 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga 2821 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg 2881 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta 2941 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc 3001 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc 3061 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 3121 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 3181 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat 3241 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 3301 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 3361 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 3421 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata 3481 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 3541 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc 3601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 3661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 3721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 3781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 3841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 3901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 3961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 4021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 4081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 4141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 4201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 4261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 4321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 4381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 4441 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 4501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 4561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 4621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 4681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 4741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 4801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 4861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 4921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 4981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 5041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 5101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 5161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 5221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 5281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 5341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 5401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 5461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 5521 aataggggtt ccgcgcacat ttccccgaaa agtgccacct g //
pCAGGFP载体图谱质粒图谱pdf版和相关资料下载
pCAGGFP载体质粒应用举例
- 上一篇:pY001 (pFnCpf1_full)
- 下一篇:pEF-GFP