| 质粒名称: | SPDK3876(TRV-RNA2-pPEBV-MCS) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 149275 |
| 存储实验室: | Savithramma Dinesh-Kumar |
|
载体骨架基本信息 |
|
| 载体名称: | pCAMBIA0390 T-DNA vector |
| 载体出品公司: | -- |
| 空载体大小: | 6426 bp |
| 完整质粒大小: | 9587 bp |
| 载体修饰: | -- |
| 载体类型: | T-DNA载体 |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PEBV启动子修饰TRV RNA2 |
| 物种来源: | 用PEBV启动子修饰烟草Rattle病毒RNA2 |
| 插入大小: | 2030 bp |
| 基因突变: | PEBV外壳蛋白序列中的T2318C（DNA） |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XbaI、StuI、BamHI、KpnI、SacI、MluI、XhoI、XmaI/SmaI（未知破坏） |
| 3’克隆位点: | -- |
| 5’测序引物 | CATAATTATACTGATTTGTCTCTCG |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | SPDK3876(TRV-RNA2-pPEBV-MCS) was a gift from Savithramma Dinesh-Kumar (Addgene plasmid # 149275 ; http://n2t.net/addgene:149275 ; RRID:Addgene_149275) |
| 参考文献部分: | Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs. Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas DF. Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 10.1038/s41477-020-0670-y. 10.1038/s41477-020-0670-y PubMed 32483329 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS SPDK3876(TRV-RNA2-pPEBV-MCS) 10927 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Modified Tobacco rattle virus RNA2 vector that could be used for
expression of proteins or RNAs.
ACCESSION .
VERSION .
KEYWORDS SPDK3876(TRV-RNA2-pPEBV-MCS)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10927)
AUTHORS Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
DF
TITLE Multiplexed heritable gene editing using RNA viruses and mobile
single guide RNAs.
JOURNAL Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi:
10.1038/s41477-020-0670-y.
PUBMED 32483329
REFERENCE 2 (bases 1 to 10927)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10927
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 427..772
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 715..735
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
CDS 2857..2874
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 2909..3161
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 3183..3207
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 4508..5137
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 5566..6639
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 6705..6899
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(7135..7157)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 7243..7383
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 7298..7317
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(7398..7415)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7569..8157)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7649..7668)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9582..10376)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 10801..10825
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
ORIGIN
1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
2341 acgagtctag aaggcctcca tggggatccg gtaccgagct cacgcgtctc gaggcccggg
2401 catgtcccga agacattaaa ctacggttct ttaagtagat ccgtgtctga agttttaggt
2461 tcaatttaaa cctacgagat tgacattctc gactgatctt gattgatcgg taagtctttt
2521 gtaatttaat tttctttttg attttatttt aaattgttat ctgtttctgt gtatagactg
2581 tttgagatcg gcgtttggcc gactcattgt cttaccatag gggaacggac tttgtttgtg
2641 ttgttatttt atttgtattt tattaaaatt ctcaacgatc tgaaaaagcc tcgcggctaa
2701 gagattgttg gggggtgagt aagtactttt aaagtgatga tggttacaaa ggcaaaaggg
2761 gtaaaacccc tcgcctacgt aagcgttatt acgcccgtct gtacttatat cagtacactg
2821 acgagtccct aaaggacgaa acgggagaac gctagccacc accaccacca ccacgtgtga
2881 attacaggtg accagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
2941 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
3001 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
3061 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
3121 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattaa actatcagtg
3181 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
3241 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
3301 cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc
3361 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
3421 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
3481 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
3541 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
3601 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
3661 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
3721 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
3781 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
3841 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
3901 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
3961 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
4021 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
4081 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
4141 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
4201 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
4261 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc
4321 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
4381 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
4441 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
4501 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
4561 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
4621 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
4681 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
4741 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
4801 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
4861 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
4921 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
4981 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
5041 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
5101 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
5161 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
5221 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
5281 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
5341 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
5401 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
5461 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgcctg
5521 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa
5581 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
5641 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
5701 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
5761 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
5821 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
5881 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
5941 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
6001 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
6061 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
6121 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
6181 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
6241 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
6301 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
6361 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
6421 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
6481 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
6541 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
6601 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
6661 caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac
6721 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
6781 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
6841 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
6901 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
6961 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
7021 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
7081 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
7141 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
7201 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
7261 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
7321 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
7381 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
7441 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
7501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
7561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
7621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
7681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
7741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
7801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
7861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
7921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
7981 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
8041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
8101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
8161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
8221 ctgatgaatc ccctaatgat ttttatcaaa atcattaagt taaggtagat acacatcttg
8281 tcatatgatc aaatggtttc gccaaaaatc aataatcaga caacaaaatg tgcgaactcg
8341 atattttaca cgactctctt taccaattct gccccgaatt acacttaaaa cgactcaaca
8401 gcttaacgtt ggcttgccac gccttacttg actgtaaaac tctcactctt accgaacttg
8461 gccgtaacct gccaaccaaa gcgagaacaa aacataacat caaacgaatc gaccgattgt
8521 taggtaatcg tcacctccac aaagagcgac tcgctgtata ccgttggcat gctagcttta
8581 tctgttcggg caatacgatg cccattgtac ttgttgactg gtctgatatc cgtgagcaaa
8641 aacggcttat ggtattgcga gcttcagtcg cactacacgg tcgttctgtt actctttatg
8701 agaaagcgtt cccgctttca gagcaatgtt caaagaaagc tcatgaccaa tttctagccg
8761 accttgcgag cattctaccg agtaacacca caccgctcat tgtcagtgat gctggcttta
8821 aagtgccatg gtataaatcc gttgagaagc tgggttggta ctggttaagt cgagtaagag
8881 gaaaagtaca atatgcagac ctaggagcgg aaaactggaa acctatcagc aacttacatg
8941 atatgtcatc tagtcactca aagactttag gctataagag gctgactaaa agcaatccaa
9001 tctcatgcca aattctattg tataaatctc gctctaaagg ccgaaaaaat cagcgctcga
9061 cacggactca ttgtcaccac ccgtcaccta aaatctactc agcgtcggca aaggagccat
9121 ggattctagc aactaactta cctgttgaaa ttcgaacacc caaacaactt gttaatatct
9181 attcgaagcg aatgcagatt gaagaaacct tccgagactt gaaaagtcct gcctacggac
9241 taggcctacg ccatagccga acgagcagct cagagcgttt tgatatcatg ctgctaatcg
9301 ccctgatgct tcaactaaca tgttggcttg cgggcgttca tgctcagaaa caaggttggg
9361 acaagcactt ccaggctaac acagtcagaa atcgaaacgt actctcaaca gttcgcttag
9421 gcatggaagt tttgcggcat tctggctaca caataacaag ggaagactca ctcgtggctg
9481 caaccctgct tactcaaaat ctattcacac atggttacgt tttggggaaa ttatgagggg
9541 atctctcagc gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt
9601 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
9661 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
9721 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
9781 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
9841 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
9901 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
9961 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
10021 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
10081 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
10141 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
10201 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
10261 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
10321 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
10381 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
10441 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
10501 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
10561 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
10621 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
10681 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
10741 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
10801 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
10861 gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg
10921 gcgcgcc
//
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