| 质粒名称: | pEGFP-AQP9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 48808 |
| 存储实验室: | Kenneth Jacobson, Vesa Loitto |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4731 bp |
| 完整质粒大小: | 5610 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | AQP9,Aquaporin 9 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 888 bp |
| GenBank编号 | NM_020980 |
| 标签/融合蛋白: | eGFP（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | KpnI(未破坏) |
| 3' 克隆位点: | BamHI (未破坏) |
| 5' 测序引物 | atcacatggtcctgctggag |
|
文献引用方法 |
|
| 材料和方法部分: | pEGFP-AQP9 was a gift from Kenneth Jacobson & Vesa Loitto (Addgene plasmid # 48808 ; http://n2t.net/addgene:48808 ; RRID:Addgene_48808) |
| 参考文献部分: | Filopodia are induced by aquaporin-9 expression. Loitto VM, Huang C, Sigal YJ, Jacobson K. Exp Cell Res. 2007 Apr 15;313(7):1295-306. Epub 2007 Feb 8. 10.1016/j.yexcr.2007.01.023 PubMed 17346701 |
买家导航
pEGFPAQP9载体基本信息
pEGFPAQP9载体图谱质粒图谱和多克隆位点信息
pEGFPAQP9载体简介
pEGFPAQP9载体序列
LOCUS pEGFP-AQP9 5610 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Express human AQP9, which induces filopodia formation.
ACCESSION .
VERSION .
KEYWORDS pEGFP-AQP9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5610)
AUTHORS Loitto VM, Huang C, Sigal YJ, Jacobson K
TITLE Filopodia are induced by aquaporin-9 expression.
JOURNAL Exp Cell Res. 2007 Apr 15;313(7):1295-306. Epub 2007 Feb 8.
PUBMED 17346701
REFERENCE 2 (bases 1 to 5610)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5610
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 607..616
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 613..1329
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 613..1329
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(658..679)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(919..938)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1266..1287
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 2398..2519
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2435..2454)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2489..2508
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2526..2981)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2663..2684)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2875..2894
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3008..3112
/gene="bla"
/label=AmpR promoter
promoter 3114..3471
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3137..3157)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3322..3457
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3384..3403
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3506..4300
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3560..3579)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4170..4189
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4488..4507)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4532..4579
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4908..5496
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5397..5416
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
1381 atgcagcctg agggagcaga aaagggaaaa agcttcaagc agagactggt cttgaagagc
1441 agcttagcga aagaaaccct ctctgagttc ttgggcacgt tcatcttgat tgtccttgga
1501 tgtggctgtg ttgcccaagc tattctcagt cgaggacgtt ttggaggggt catcactatc
1561 aatgttggat tttcaatggc agttgcaatg gccatttatg tggctggcgg tgtctctggt
1621 ggtcacatca acccagctgt gtctttagca atgtgtctct ttggacggat gaaatggttc
1681 aaattgccat tttatgtggg agcccagttc ttgggagcct ttgtgggggc tgcaaccgtc
1741 tttggcattt actatgatgg acttatgtcc tttgctggtg gaaaactgct gatcgtggga
1801 gaaaatgcaa cagcacacat ttttgcaaca tacccagctc cgtatctatc tctggcgaac
1861 gcatttgcag atcaagtggt ggccaccatg atactcctca taatcgtctt tgccattttt
1921 gactccagaa acttgggagc ccccagaggc ctagagccca ttgccatcgg cctcctgatt
1981 attgtcattg cttcctccct gggactgaac agtggctgtg ccatgaaccc agctcgagac
2041 ctgagtccca gacttttcac tgccttggca ggctgggggt ttgaagtctt cagagctgga
2101 aacaacttct ggtggattcc tgtagtgggc cctttggttg gtgctgtcat tggaggcctc
2161 atctatgttc ttgtcattga aatccaccat ccagagcctg actcagtctt taaggcagaa
2221 caatctgagg acaaaccaga gaaatatgaa ctcagtgtca tcatgtaggg atccaccgga
2281 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
2341 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
2401 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
2461 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
2521 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
2581 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
2641 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
2701 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
2761 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
2821 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
2881 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
2941 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
3001 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
3061 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
3121 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
3481 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
3541 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
3601 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
3661 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
3721 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
3781 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
3841 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
3901 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
3961 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
4021 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
4081 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
4141 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
4201 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
4261 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
4321 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
4381 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
4441 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
4501 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
4561 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
4621 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
4681 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
4741 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
4801 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
4861 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
4921 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
4981 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
5041 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
5101 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
5161 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
5221 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
5281 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
5341 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
5401 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
5461 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
5521 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
5581 tctgtggata accgtattac cgccatgcat
//
