| 质粒名称: | SQT1665,pSQT1665-LbCpf1-NLS-3×HA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 78744 |
| 存储实验室: | Keith Joung |
|
载体骨架基本信息 |
|
| 载体名称: | pCAG-GFP |
| 载体出品公司: | Cepko lab (Addgene plasmid# 11179) |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA,Cpf1 |
| 物种来源: | Lachnospiraceae bacterium ND2006 |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CAG |
| 标签/融合蛋白: |
NLS（插接件上的C端子）
3x HA（插入件上的C端子）
|
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3') |
| 3' 测序引物 | M13-rev |
|
文献引用方法 |
|
| 材料和方法部分: | SQT1665 was a gift from Keith Joung (Addgene plasmid # 78744 ; http://n2t.net/addgene:78744 ; RRID:Addgene_78744) |
| 参考文献部分: | Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK. Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620. 10.1038/nbt.3620 PubMed 27347757 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS SQT1665 8653 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Human expression plasmid for LbCpf1-NLS-3xHA.
ACCESSION .
VERSION .
KEYWORDS SQT1665
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8653)
AUTHORS Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM,
McCaw ZR, Aryee MJ, Joung JK
TITLE Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human
cells.
JOURNAL Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.
PUBMED 27347757
REFERENCE 2 (bases 1 to 8653)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8653
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 386..661
/label=chicken beta-actin promoter
intron 662..1670
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1594..1616
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1678..1697
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 1769..5452
/codon_start=1
/product="CRISPR-associated protein from Lachnospiraceae
bacterium ND2006 (Zetsche et al., 2015)"
/label=LbCpf1
/translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
VKH"
CDS 5453..5500
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 5507..5587
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
primer_bind complement(5677..5696)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 5742..5797
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(5796..5815)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(6156..6172)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6156..6172)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6169..6191)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6180..6196
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6204..6234)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6249..6270
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6329..6524
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 6375..6510
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6437..6456
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 6530..6664
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6567..6586)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6621..6640
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6732..6749)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6903..7491)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6983..7002)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7662..8522)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8285..8304
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8523..8627)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
1741 gcgggcccgg gatccaccgg tcgccaccat gagcaagctg gagaagttta caaactgcta
1801 ctccctgtct aagaccctga ggttcaaggc catccctgtg ggcaagaccc aggagaacat
1861 cgacaataag cggctgctgg tggaggacga gaagagagcc gaggattata agggcgtgaa
1921 gaagctgctg gatcgctact atctgtcttt tatcaacgac gtgctgcaca gcatcaagct
1981 gaagaatctg aacaattaca tcagcctgtt ccggaagaaa accagaaccg agaaggagaa
2041 taaggagctg gagaacctgg agatcaatct gcggaaggag atcgccaagg ccttcaaggg
2101 caacgagggc tacaagtccc tgtttaagaa ggatatcatc gagacaatcc tgccagagtt
2161 cctggacgat aaggacgaga tcgccctggt gaacagcttc aatggcttta ccacagcctt
2221 caccggcttc tttgataaca gagagaatat gttttccgag gaggccaaga gcacatccat
2281 cgccttcagg tgtatcaacg agaatctgac ccgctacatc tctaatatgg acatcttcga
2341 gaaggtggac gccatctttg ataagcacga ggtgcaggag atcaaggaga agatcctgaa
2401 cagcgactat gatgtggagg atttctttga gggcgagttc tttaactttg tgctgacaca
2461 ggagggcatc gacgtgtata acgccatcat cggcggcttc gtgaccgaga gcggcgagaa
2521 gatcaagggc ctgaacgagt acatcaacct gtataatcag aaaaccaagc agaagctgcc
2581 taagtttaag ccactgtata agcaggtgct gagcgatcgg gagtctctga gcttctacgg
2641 cgagggctat acatccgatg aggaggtgct ggaggtgttt agaaacaccc tgaacaagaa
2701 cagcgagatc ttcagctcca tcaagaagct ggagaagctg ttcaagaatt ttgacgagta
2761 ctctagcgcc ggcatctttg tgaagaacgg ccccgccatc agcacaatct ccaaggatat
2821 cttcggcgag tggaacgtga tccgggacaa gtggaatgcc gagtatgacg atatccacct
2881 gaagaagaag gccgtggtga ccgagaagta cgaggacgat cggagaaagt ccttcaagaa
2941 gatcggctcc ttttctctgg agcagctgca ggagtacgcc gacgccgatc tgtctgtggt
3001 ggagaagctg aaggagatca tcatccagaa ggtggatgag atctacaagg tgtatggctc
3061 ctctgagaag ctgttcgacg ccgattttgt gctggagaag agcctgaaga agaacgacgc
3121 cgtggtggcc atcatgaagg acctgctgga ttctgtgaag agcttcgaga attacatcaa
3181 ggccttcttt ggcgagggca aggagacaaa cagggacgag tccttctatg gcgattttgt
3241 gctggcctac gacatcctgc tgaaggtgga ccacatctac gatgccatcc gcaattatgt
3301 gacccagaag ccctactcta aggataagtt caagctgtat tttcagaacc ctcagttcat
3361 gggcggctgg gacaaggata aggagacaga ctatcgggcc accatcctga gatacggctc
3421 caagtactat ctggccatca tggataagaa gtacgccaag tgcctgcaga agatcgacaa
3481 ggacgatgtg aacggcaatt acgagaagat caactataag ctgctgcccg gccctaataa
3541 gatgctgcca aaggtgttct tttctaagaa gtggatggcc tactataacc ccagcgagga
3601 catccagaag atctacaaga atggcacatt caagaagggc gatatgttta acctgaatga
3661 ctgtcacaag ctgatcgact tctttaagga tagcatctcc cggtatccaa agtggtccaa
3721 tgcctacgat ttcaactttt ctgagacaga gaagtataag gacatcgccg gcttttacag
3781 agaggtggag gagcagggct ataaggtgag cttcgagtct gccagcaaga aggaggtgga
3841 taagctggtg gaggagggca agctgtatat gttccagatc tataacaagg acttttccga
3901 taagtctcac ggcacaccca atctgcacac catgtacttc aagctgctgt ttgacgagaa
3961 caatcacgga cagatcaggc tgagcggagg agcagagctg ttcatgaggc gcgcctccct
4021 gaagaaggag gagctggtgg tgcacccagc caactcccct atcgccaaca agaatccaga
4081 taatcccaag aaaaccacaa ccctgtccta cgacgtgtat aaggataaga ggttttctga
4141 ggaccagtac gagctgcaca tcccaatcgc catcaataag tgccccaaga acatcttcaa
4201 gatcaataca gaggtgcgcg tgctgctgaa gcacgacgat aacccctatg tgatcggcat
4261 cgataggggc gagcgcaatc tgctgtatat cgtggtggtg gacggcaagg gcaacatcgt
4321 ggagcagtat tccctgaacg agatcatcaa caacttcaac ggcatcagga tcaagacaga
4381 ttaccactct ctgctggaca agaaggagaa ggagaggttc gaggcccgcc agaactggac
4441 ctccatcgag aatatcaagg agctgaaggc cggctatatc tctcaggtgg tgcacaagat
4501 ctgcgagctg gtggagaagt acgatgccgt gatcgccctg gaggacctga actctggctt
4561 taagaatagc cgcgtgaagg tggagaagca ggtgtatcag aagttcgaga agatgctgat
4621 cgataagctg aactacatgg tggacaagaa gtctaatcct tgtgcaacag gcggcgccct
4681 gaagggctat cagatcacca ataagttcga gagctttaag tccatgtcta cccagaacgg
4741 cttcatcttt tacatccctg cctggctgac atccaagatc gatccatcta ccggctttgt
4801 gaacctgctg aaaaccaagt ataccagcat cgccgattcc aagaagttca tcagctcctt
4861 tgacaggatc atgtacgtgc ccgaggagga tctgttcgag tttgccctgg actataagaa
4921 cttctctcgc acagacgccg attacatcaa gaagtggaag ctgtactcct acggcaaccg
4981 gatcagaatc ttccggaatc ctaagaagaa caacgtgttc gactgggagg aggtgtgcct
5041 gaccagcgcc tataaggagc tgttcaacaa gtacggcatc aattatcagc agggcgatat
5101 cagagccctg ctgtgcgagc agtccgacaa ggccttctac tctagcttta tggccctgat
5161 gagcctgatg ctgcagatgc ggaacagcat cacaggccgc accgacgtgg attttctgat
5221 cagccctgtg aagaactccg acggcatctt ctacgatagc cggaactatg aggcccagga
5281 gaatgccatc ctgccaaaga acgccgacgc caatggcgcc tataacatcg ccagaaaggt
5341 gctgtgggcc atcggccagt tcaagaaggc cgaggacgag aagctggata aggtgaagat
5401 cgccatctct aacaaggagt ggctggagta cgcccagacc agcgtgaagc acaaaaggcc
5461 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc catacgatgt
5521 tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga
5581 ctatgcctaa agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
5641 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
5701 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
5761 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
5821 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg
5881 ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg
5941 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
6001 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
6061 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat
6121 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
6181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
6241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
6301 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
6361 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
6421 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
6481 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
6541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
6601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
6661 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
6721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
6781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
6841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
6901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
6961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
7021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
7081 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
7141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
7201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
7261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
7321 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
7381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
7441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
7501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
7561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
7621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
7681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
7741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
7801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
7861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
7921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
7981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
8041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
8101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
8161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
8221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
8281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
8341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
8401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
8461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
8521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
8581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
8641 aaagtgccac ctg
//
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